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Preparation issues of teen-agers between the ages of 16-18 years
Smejkal, Jaroslav ; Buzek, Mario (advisor) ; Bošanský, Jiří (referee)
TITLE Preparation issues ofteen-agers between the ages of 16 - 18 years GOAL OF THE WORK The goals of my work were to outline the training model in the Czech Republic, monitoring of players complex effort of FC Victoria Pilsen, next the detection of negatives in game activities in matches and suggestion the training steps to removing realized negatives. METHODS Research was realized by direct and indirect observation methods, mainly using DVD records of teenagers 1st match. There were used qualitative and quantitative analyses of game activities. RESULTS Analysis should reveal some new facts. Results should define requirements for improvement oftechnical and tactical maturity ofindividua! in activities. KEYWORDS Game effort, qualitative and quantitative analysis, errors solution 3
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The nursing process in a patient with cystic fibrosis
Václavková, Aleksandra ; Tomová, Šárka (advisor) ; Kaplická, Jitka (referee)
This bachelor ́s thesis deals with patients suffering from cystic fibrosis (CF). The thesis includes a theoretical and a practical part. In the theoretical one, underlying anatomical and physiological disturbances in key organ systems are discussed. A historical review, genetics aspects, manifestations of the disease and current treating possibilities are also concerned. Despite a considerable improvement on the field of the diagnostic process, the diagnosis of CF is still often considered to late with negative consequences on the prognosis. Although the causal therapy is still not existing, an intensive supportive care can significantly prolong and improve the quality of life of the patients. Considerable progress in the treatment research promises a total cure in the future. The practical part of the thesis contains an analysis of separates steps of the nursing process and its practical application in one concrete patient. I elaborated a nursing anamnesis according to the Gordon's functional health model. I elicited detailed information about patient's health state for establishing his complex evaluation. When the nursing problems determined and the diagnoses established, I worked on the realisation of defined nursing goals. According to the efficacy feedback, I continuously adapted the approach to the...
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Testing of the Applicability of Anewly Developed Anti-c-Myb Antibody
Pleschnerová, Markéta ; Šnajdr, Pavel (advisor) ; Špunda, Miloslav (referee)
Specific molecules present in cell enables to determine its properties such as function, origin, stage of differentiation. Antigenic features of these molecules are used for their detection, especially in biomedical research and diagnostics. Specific bond between antigen and antibody can be proved by imunological methods e.g. ELISA or Western Blot. Applicability of the antibody for cell and tissue antigens needs to be tested directly by immunocytochemistry and immunohistochemistry. Each antigen requires specific ways of fixation, processing or retrieval. These are the crucial steps for successful antigen detection. Other important steps are appropriate antibody dilution and duration and temperature of incubation. The aim of this work was to determine the applicability of newly developed antic- Myb antibody, obtained by immunization of chick with a fragment of murine c-Myb protein. Blood serum obtained from the same animal before immunization was used as a negative control. Western Blot was applied to verify the specificity of our antibody. Its application for immunocytochemistry was succesfully proved on c-Myb transfected murine embryonic fibroblasts. Results of in situ hybridization (ISH) of c-myb mRNA in whole mount murine embryos compared to published data were used to define prospective c- Myb protein...
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Current Situation with the ADHD/ADP Syndrom Children
MARKOVÁ, Marie
The thesis deals with the problematic of specific behavior disorders. Despite the age some children keep suffering from complications which negatively influence their studies in the form of insufficient reading techniques, in the lower ability of text understanding and consequently disability to study the read material. The goal of the thesis is to suggest a number of recommendations how to help those children in the phrame of their integration to a common basic schoul course. Separate steps (form and frequency of correction, contents of corrections) are described in the individual educational plan.
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The Role of Lipid Rafts in Translocation of the Adenylate Cyclase Toxin of B. pertussis across Cytoplasmatic Membrane
Chvojková, Věra ; Gahura, Ondřej (referee) ; Šebo, Peter (advisor)
The bacterium Bordetella pertussis is the causative agent of pertussis or whooping cough. The main virulent factor of this gram-negative bacterium is adenylate cyclase toxin, a member of the RTX protein family. After secretion, the toxin binds to CD11b/CD18 integrin receptor of myeloid phagocytic cells and consequently translocates its adenylate cyclase enzyme (AC) into the cytosol of the host cells. In the cytosol, the AC is activated by calmodulin, followed by rapid conversion of ATP into the signaling molecule cAMP, resulting in paralysis of bactericidal functions of phagocytic cells. Recently it was shown that translocation of the catalytic AC domain into the cytosol proceeds in two steps. After binding of CyaA to the receptor, the influx of calcium ions into the cell occurs. High local concentration of calcium induces the translocation of the CyaA- CD11b/CD18 complex into the lipid raft, where the translocation of adenylate cyclase enzyme into cytosol occurs. This work is aiming on the description of the new established paradigm dealing with membrane translocation of RTX toxins.
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CUG Codon in Pathogenic Yeasts of the Genus Candida
Marečková, Lucie ; Půta, František (referee) ; Heidingsfeld, Olga (advisor)
2. Abstract In many Candida species the standard leucine CUG codon is translated as a serine, although not in 100% cases. This dual specifity of the CUG codon has evolved through a mechanism that required codon ambiguity mediated by a unique tRNACAG, which is in vitro aminoacylated more often by serine than by leucine. This codon ambiguity has been tolerated for more than 170 million years. The explanation at least for now is that the CUG codon reassignment could have generated genetic diversity that facilitated occurrence of new phenotypes resistant to stress. Beside this, an important step was to reduce negative impact of the codon ambiguity by crucial mutations in the structure of the ser-tRNACAG. Candida species became a valuable experimental model for elucidation of the genetic code changes. While consequences of the CUG codon reassignment have been extensively studied in Candida albicans, this topic has not yet been addressed in Candida parapsilosis. Solving the structure of C. parapsilosis secreted proteinase Sapp1p provided a tool to carry out a "case study" of possible effects of the CUG codon ambiguity. The SAPP1 gene contains one CUG codon, and the respective serine is located on the loop in the close proximity of the active site of the proteinase.
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Software Estimation
Surňák, Peter ; Buchalcevová, Alena (advisor) ; Stanovská, Iva (referee)
This thesis deals with software estimation, describes individual methods which may be used for constructing estimates in terms of effort, time and costs. It also deals with quality of estimate, the way it is used, the process of selection of the right method and other consequences. The main goal of thesis is creating a compact overview of currently known and used methods and approaches to constructing estimates. As a secondary target comes an example of practical use of estimate on a simulated project, where the way of choosing estimating method is presented, based on project attributes such as size, current stage of project or project type. For achieving these intentions, it is important to first set up basic framework of basic terms such as estimate, estimating, project and software project. Apart from just defining these terms, there are some necessary interrelations which must be explained before anything else. Another step is creating categorization of particular methods, their description, restrictions and suitable uses. The quality of an estimate, including factors which may bias, degrade or improve it, is another step towards final goal. Consequences, which may arise when wrong estimate has been provided, are one more piece of mosaic to this work. Lastly, case study demonstrate how to choose from estimating methods and use them on particular project. The expected benefits of the thesis are mainly in summarizing of existing approaches to the art of estimating broken down to categories, as well as list of principles and factors which affects an estimate and the ways estimates influence the project (in either positive or negative direction).
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Transfer-pricing-based tax planning
Lhotský, Vladimír ; Buus, Tomáš (advisor) ; Kuncl, Karel (referee)
This work brings a systematic approach to the design of a transaction-based transfer-pricing policy of a multinational group and related tax optimization. The foundation of the work is a description and analysis of all aspect of the transfer-pricing regulation including a detailed classification of particular business risks. Using a certain simplification of the complicated relations in multinational groups, a system compliant with transfer-pricing rules is designed, hence minimizing the tax risk. Further, I devote my attention to rather complicated areas of transfer-pricing like pricing of payments for intangible property, pricing in non-standard structures and allocation of exchange rate risk. After the build-up of the architecture of the transfer-pricing system, analysis of the tax optimization possibilities adhering to all regulatory requirements is carried out. The detailed description of the tax effective supply chain management planning concept and other planning instruments is presented. The final part is devoted to the negative tax implications of business restructuring steps analyzed on the examples of particular structures.
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Flat tax
Spišský, Jakub ; Marková, Hana (advisor) ; Boháč, Radim (referee)
RESUMÉ Application of flat tax in the Slovak Republic causes better allocation of income to state budget and reduces rate of repartitionings of income in the society. Moreover, from a long-time aspect, it stimulates economic growth and creation of investments. From an individual's and companies' point of view, the flat tax eliminates progressive taxation, which up to now affected mainly those who enjoyed success - those with higher income in the society. On the other hand, the flat tax implementation requires that the more successful companies or individuals pay less and the less successful ones pay more than they would under the progressive tax system, for the state to raise the same amount of money. Implementation of the flat tax instead of the progressive taxationis an important step in the right direction, but deffinitely not the final station. There is still a lot of discussion going on about the fairness of this form of taxation. It is not entirely true that everyone who works and earns income deduct from their income 19%. The reason for this is the system of exemptions - nondeductable minimum and the tax bonus for a child, which makes it possible to reach a negative tax. The higher the nondeductable minimum, the higher the taxation differences. Flat rate significantly decreased progressivity in the...
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Steps of the European Comission to Reduce the CO2 Emissions from Passenger Cars
Nováková, Hana ; Geuss, Erik (advisor) ; Branžovský, Antonín (referee)
The Bachelor Degree Thesis deals with the air pollution and emissions of CO2 from passenger cars. The EU signed the Kyoto protocol, which binds the member states to reduce emission of CO2 by 8% against the year 1990. While is reducing in other sectors succesfull, emissions in transport keep rising. That is why the European Comission decided to create a legislation to reach the average fleet emission target of 130 g/km with the motor vehicle technology and next 10 grams with other improvements like biofuels, ecodriving, better infrastructure or traffic lights. It means that in 2012 the fleet of a manufacturer should emit 120 g/km in average. The European Comission concerns with how to make the proposal equitable to all car manufacturers. The emission limit will probably be set by the weight of the car with the motivation of reducing the weight of cars. The thesis describes also negative social impacts, which the propsal might have. An opinion of many interested parties was taken into account. The exact legislation should be proposed soon.
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