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Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
Doubková, Martina ; Bačáková, Lucie ; Pařízek, Martin ; Vandrovcová, Marta ; Musílková, Jana ; Lisá, Věra ; Gabor, R. ; Marvan, J.
Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants.
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Biochemical aspect of investigation of the natural-origin binders and varnishes in the paint layer of old paintings
Pecháčková, Soňa ; Kubíčková, Božena (referee) ; Hudeček, Jiří (advisor)
The subject of this work are choosen bioanalytical methods, used for the identification of organic binders and varnishes in coloured layer of ancient paintings. In the past, the plant and animal substances were used as a binders and varnishes. To those materials belong proteins, oils, polysacharide gums and resins. Succesful analysis in the history of using binders and varnishes demands a good knowledge of their chemical qualities and reactivity during the process of aging. Nowadays are prefered the methods and technics, which don?t destruct the sample and which are non-invasive towards the whole artwork. Recently, the imunofluorescence technics have been started to use as the alternative methods of classical and easier microchemical tests. The spectroscopic methods are widely used in material investigation of ancient artworks. The main is infrared spectometry (FTIR) and Raman spectroscopy. The methods of nuclear magnetic resonance are significant mainly for studying changes of connective and isolating material during the process of aging. To indentify mainly protein binders, the technic of mass spectrometry (MALDI-TOF) is widely used. Due to the progress of technic the paper and thin-layer chromatography were replaced by very effective high performance liquid chromatography (HPLC), gas chromatography (GC)...
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The nation-wide Czech language leaving examination for deaf students
Andrejsek, Jan ; Pacovská, Jasňa (advisor) ; Hudáková, Andrea (referee)
This bachelor work focuses on the new conception of Czech leaving examination of prelingually deaf students guaranteed by the state. The author describes the old form of Czech language and literature leaving examination, which were the deaf students obliged to pass till nowadays, and also the creation and development of adaptation of Czech language leaving examination for the deaf. By describing the current situation, the author shows us the creation of new conception of leaving examination in its historical framework. Description of this new conception is related to the needs of students whom were admitted special conditions of taking leaving examination, because the prelingually deaf students also belongs to this category. The profound analysis of actual adaptation of Czech language leaving examination for the deaf is followed by the statements of its probably positive impact for prelingually deaf students education in the future. The possible problems related to this new examination, which cannot be leaved out, are also mentioned.
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Freshwater bryozoa in Úslava River at localities Starý Plzenec and Koterov
Steinerová, Michaela ; Vymazal, Jan (advisor) ; Magdalena, Magdalena (referee)
Freshwater bryozoans belong to a group of colonial invertebrates. Bryozoa in Czech translation means moss and animal. Representatives of bryozoans, are at first glance very similar to moss and plants from other species differ considerably.
The main objective of this thesis is to determine the different species found in the territories Koterov and Old Plzenec, to check the ecological demands of species found, to evaluate the seasonal dynamics of occurrence bryozoans and to compare the data with the available literature sources.
The monitored stretch of the river Úslava has been divided into 10 sites. Five locations were explored on the Koterov territory and on the territory of Old Plzenec another 5 locations aswell. At both locations, a total of 150 samples of these bryozoans have been collected and determined 233 zoarias.
Total number of 5 species of freshwater bryozoans were founded in the observed area Koterov - Starý Plzenec: Plumatella emarginata (Allman, 1844), Plumatella fungosa (Pallas, 1768), Plumatella repens (Linné, 1758), Fredericella sultana (Blumenbach, 1779) a Paludicella articulata (Ehrenberg, 1831).
Individual species were determined by a synoptical key from athors Wood and Okamura (2005)
From the obtained results, there has been evaluated a seasonal dynamics of the representation of individual species during July, August and September. In addition to the seasonal dynamics, light requirements and preferences of the species and the occurences of particular species were evaluated.
In the Koterov area, Plumatella emarginata has occured most widely, second common type was Plumatella fungosa. There were less abundant species of Fredericella sultana and Paludicella articulata. A type Plumatella repens was found only rarely. on the teritorry of Old Plzenec, most frequently Plumatella fungosa did appear, secondly, Plumatella emarginata. The least recorded species were Plumatella repens and Fredericella sultana.
Most frequently, all the species were occiring on the top and sides of the stones. Plumatela fungosa, Plumatella emarginata and Paludicella articulata were mostly recorded on the upper and lateral sides of the stones. Types Fredericella sultana and Plumatella repens were plentiful on the upper sides of the stones. The reason for this occurrence on the upper side could be a rich food supply. On the flank, the species are probably well protected from predators. The undersides of the stones were inhabitated rarely, probably due to insufficient supply of the oxygen.
My observation of Paludicella articulata being one of the shade-liking species are in line with the findings from the availiable literature . The other species were found both at shaded and sunlit sites and it cannot be percisely determined, if teh lighting conditions have major role in their development.
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Epidemiology and pathogenesis of Escherichia coli
HUBKOVÁ, Petra
Escherichia coli is one of the most important representatives of intestinal microflora. It contributes to the proper course of digestive processes in the gut, creating a beneficial cooperation between microorganism and macroorganism. But some strains may be pathogenic. The scope of my thesis is to get deeply acquainted with the E. coli bacteria, beginning with characteristics, pathogenicity and pathogenesis, laboratory diagnostics and ending with the clinical picture of the disease and therapy. From an epidemiological perspective, pathogenic E. coli appears rather sporadically, but in developing countries can grow up into endemics, especially because of the lack of hygiene. Described are reservoirs, transmission routes and the most famous cases of E. coli occurrence. In the practical part, I focus on the incidence of E. coli in preterm infants, newborns and children up to 3 years old, which are examined with a diarrheal disease diagnosis. During the period from January 2015 to December 2015, 1518 stools from children aged 0 3 years were examined, whereas 25 stools were positive for dyspeptic strains of E. coli, which is the originator of diarrhea. Different non-pathological and pathological flora was proven in 725 stools and non-dyspeptic strain of E. coli was ascertained in 768 stools, which is not the originator of diarrhea. All 1518 stools were examined using classical methods of identification, such as cultivation and biochemical tests (ENTEROtest, a wide range of sugars). To determine the specific serotype (O antigen) of E. coli, serotyping (antigenic analysis of strain) was performed on examined bacterial strain using slide agglutination method. On the basis of proven E. coli serotype, results were statistically analyzed and evaluated in order to determine the strain of E. coli.
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Šlechtitelský potenciál historických odrůd jádrovin
Reichlová, Petra
Modern varieties of pome-fruit lost during breeding some important properties, such as resistance to pathogens or tolerance to worse climatic conditions. Therefore, attention of breeders turns to some old varieties and landraces, which lost its market significance, but which can still offer a wide range of properties useful in breeding of new varieties. The apple breeding aims are focused on locating and obtaining donors of genes for resistance to Venturia inaequalis (causingapple scab), Podosphaera leucotricha (powdery mildew), Erwinia amylovora (fire blight) and Candidatus Phytoplasma mali (apple proliferation). Pear breedingis focused on finding a suitable donors of resistance to Venturia pirina (pear scab), Gymnosporangium sabinae (pear rust), Erwinia amylovora (fire blight). Landraces (local varieties) were often grown under the severe climatic conditions. Over the years they get their adaptability for the environment conditions. Adaptation to these conditions, especially resistance to frost, are one of the characteristics used in breeding.
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New Labour Code {--} its positives and negatives
TENKLOVÁ, Ivana
The task of this thesis named {\clqq}New Labour Code {--} its positives and negatives`` is to compare the old Labour Code with the new one, to show the changes and updates and their impact to practice, to find the positives and negatives in its application. After long negotiations, the Labour Code began to be valid in January 2007 in a new form, but only by the {\clqq}technical novel`` and by the finding of the Constitutional court the main inadequacies were eliminated. The question remains if the Labour Code as an independent Code is necessary. It is usual in some countries that the labour-law tasks are treated in the Civil Code, but our legal regulations result from the Soviet concept of the legal system. Currently the framework of the new Civil Code is finished; one of the authors is Professor Karel Eliáš, who is just the protagonist of incorporation the labour law into the Civil Code. On the other hand, the labour law nowadays is so wide and specific, that its incorporation back into the civil law would have been extremely difficult.
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Evolution vs. Creation: Where the Truth Lies
Miklošová, Zoana ; Lhotský, Josef (advisor) ; Stella, Marco (referee)
The debate between creationism and evolution remains vital after 80 years. Creation Science, represented by Young- Earth Creationism (YEC) and Old-Earth Creationism (OEC), denies fact of macroevolution and postulates based on Genesis model of separately created species. Thesis submits brief description of creationistic model and compares it with Darwinian idea of descent with modification from common ancestor. Primarily, the scientific and methodological merits of Creation Science are examined. In last two decades the position of Creation Science was rather decreasing. On the other hand, new phenomenon Intelligent Design (ID) became widely known. Although philosophical starting-points similar as creationistic ones ID introduces new arguments for supernatural design. This work analyses main tenets of ID - specified complexity and irreducible complexity of organisms. Again, ID's arguments are judged on its explanatory and methodological merits in confrontation with evolutional concepts reflecting recent post-neodarwinism development in biology. Key words: Creationism, biological evolution, Darwinism, Neodarwinism, Intelligent design, falsifiability, methodological naturalism
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Nature opium abusing in Olomouc and Prostejov Regions ? questionnaire study.
Kučeravcová, Jitka ; Klusoňová, Hana (advisor) ; Kračmarová, Lenka (referee)
My diploma thesis concerns the problems with the natural opium abuse in Olomouc and Prostějov region. It is based on the questionnaire analysis, which was held in The Contact Centre Walhalla and The Contact Centre Želva in the period from May to July 2006. 32 clients from the both contact centres took part in this resarch (20 from Olomouc and 12 from Prostějov). Only those clients with the experience of opium abuse were added to the group of respondents. After having analysed the results, we found that the average age of Olomouc (OL) users is 25,6 years and of Prostějov (PV) users it is 24,4 years. More than a half of them are men. 36% of clients from the both centres said that they live alone. A half of OL clients have only primary education, a half of PV clients are trained. 35% of OL clients were out of work, whereas 25% of PV clients were employed. 73% of all clients were checked on hepatitis with negative result. The majority of all clients were tested on HIV with negative result in all cases. 31% have been treated from drug dependence before, now it is only 7%. The spectrum of abused drugs is relatively wide. The first illegal drug was for 77% of all clients THC and it was when they were approximately 14,9 years old. The most favourite drug is for 40% of Olomouc clients pervitin, on the other...
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