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Parasites in nutria (Myocastor coypus)
Urbanová, Beáta ; Langrová, Iva (advisor) ; Nechybová, Stanislava (referee)
The aim of this work was published data concerning parasites coypu (Myocastor coypus).
The first part describes the anatomy and the ethology of a coypu. Nutria is a relatively large rodent, native from South America. It lives near the water areas in small family groups which are lead by the old female. Its body is adapted to the life in water. Nutria is herbivores, which eats littoral and aquatic plants. It is a polyestric animal which gives birth to about six nidifugous pups. The nutria is the non-native species in the Czech Republic. The first import of nutrias was held in 1924 from Argentina. After that they escaped from the farms and due to they got into the wild.
The second part of this work characterizes parasites that have been diagnosed in nutrias. Protozoa diseases cause the coccidia and Toxoplasma. The coccidia are parasites of the genus Eimeria and belong to the most dangerous diseases for nutrias. The trematodes (Fasciola hepatica) also occur in nutrias and causing obturation and destruction of the liver. Anoplocephala sp., Hymenolepis octocornata and Rodentolepis avetjanae from tapeworms parasitize in nutria. Additionally nutrias serve as intermediate hosts of Echinococcus granulosus, Multiceps serialis and Taenia tenuicollis. The diseases, caused by parasitic nematodes from class Nematodes, are caused by parasites Trichinella spiralis, Trichuris myocastoris, the worms of genus Strongyloides (Strongyloides myopotami), and the worms from superfamily Trichostrongyloidae (Trichostrongylus retortaeformis, T. myocastoris). Among the most ectoparasitic diseases frequently belong the scabies (Myocoptes myocastori, Chirodiscoides caviae) and Demodex spp.
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Spatial development in city Čáslav before and after year 1989
Lejnarová, Tereza ; Šlemr, Jan (advisor) ; Macoun, Milan (referee)
In my bachelor thesis I investigate influence of the urban development planning before and after year 1989 through analysis of it in Čáslav. In my literature review I introduce signs of urban development planning in the Czech lands before year 1989 in the context of the communist centrally planned economy and consecutive transformation to the capitalistic market economy after year 1989. I describe changes apparent in Čáslav which are based on the differences in the urban development planning before and after year 1989. To precisely collect these changes, I used methods of qualitative research which I describe in detail in the thesis. I chose interviews as my main research method. I interviewed people who participated in the spatial development planning in Čáslav. In my thesis I compared literature on the planning before and after year 1989 and information about changes in the town with people who participated on them and whose perception of them may differ from official statement. The goal of my thesis is to describe the urban development planning in Čáslav before and after year 1989 and perception of differences in it by people who participated in it.
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Influence of term of winter rapeseed (Brassica napus) desiccation on yield and seed quality
Rajtmajer, Stanislav ; Bečka, David (advisor) ; Petr, Petr (referee)
Rapeseed is the world's third most important oil plant (the second seminal). It is the most cultivation and most important oil plant in Czech Republic. Winter rapeseed reaches about 85% of the harvest area of oilseeds in the country. The desiccation of oilseed crop is sphere of agricultural engineering, which is still worth discussing. The main problems are how to use the product, what dose and which dates to choose to desiccate. The results of this thesis could help to partially clarify this complex issue.
The aim of the thesis is to observe the effect of different terms oilseed rape desiccation by glyphosate on yield and seed quality.
Small-plot experiments to investigate the influence of the term desiccation of winter rapeseed were established in the years 2013/2014 and 2014/2015 on the lands of the experimental station of the Czech Agricultural University, Faculty of Agronomy Food and Natural Resources at the Červený Újezd.
In the first experimental year 2013/2014 was founded five variants in three replications (including undesiccated control). In the year 2014/2015 was founded seven variants in four replicates (including undesiccated controls). In the first experimental year was the first term desiccation 16. 6. 2014, the second year 8. 6. 2015. Subsequent periods of desiccation were a week apart.
The variety of winter rapeseed used for the experiments was a hybrid variety Rohan. The desiccating agent was used Dominator active substance glyphosate. The dose of
4 l / ha + 200 l H2O.
For all samples, both experimental, years yield was determined, the weight of a thousand seeds and oiliness. For desiccated variants were carried out pre-harvest analysis for the determination of solids in pods. Further, all harvested samples taken for laboratory germination test (Determination of germination) ISTA according to the methodology. The results of the experiment sprouting were statistically analyzed using ANOVA analysis of variance. Differences between mean values were evaluated by Tukey test, the computer program SAS at a significance level of p = 0.05.
Effect of desiccation term influences of winter oilseed rape seed quality in terms of weight and thousands of seeds in terms of oil content. Too early desiccation (46 to 39 days before harvest) HTS reduced by 7-17% and the oil content of 2-4% of the overall average. Oiliness of the observed characters minimum interference term desiccation. Desiccation in the optimum date (17 days before harvest) increased oiliness of 1-6%.
Influence term desiccation oilseed rape also greatly influences seed yield.
Too early desiccation (46 to 39 days before harvest) reduced the yield by 11-14%. Undesiccated control in both years achieved the highest yields, increase yield by 5-12%. Desiccation in the optimum date (17 days before harvest) increased the yield of attempts by 5-6%.
The term desiccation of winter rapeseed, also significantly affects the vitality of seeds, where very reduces energy germinating seedlings in the first days. The term desiccation, however, does not affect overall seed germination. Too early desiccation (46 to 39 days before harvest) EK2 decreased by 12-40%, EK3 decreased by 4-24%, 3-4% EK4 and extended MGT of 7-15%. In the first experimental year was the most vital option undesiccated control (EK2 = 50.4% = 91.3% EK3, EK4 = 97.9%). In the second experimental year was the most vital seeds of the optimal term desiccation, (Sixth term, 17 days before harvest) = 68.9% EK2, EK3 = 98.2%, EK4 = 100%. Seed samples of 2014/15 had a higher overall vitality of seeds, than samples from 2013/14, the overall vitality is probably worse given year old and transsilaged seed.
The results of the two-year experiment that term desiccation affects the quality of seeds, the yield of seeds and vitality of seeds. Pre-analysis was determined optimum solids content in siliques desiccated samples to values of 40-50%. Like most technology seems to desiccation in the optimum date (17 days before harvest) and agro technology without desiccated vegetation. As the least appropriate technology seems very early desiccation (46 to 30 days before harvest).
The first scientific hypothesis: Premature desiccation reduces seed yield, oil content and HTS. Yes, the hypothesis was confirmed.
The second scientific hypothesis: Desiccation made in the correct term do not affect the quality of the seeds (oiliness and HTS). Yes, the hypothesis was confirmed.
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Trend of beer trade
Kolář, Petr ; Šánová, Petra (advisor) ; Markéta, Markéta (referee)
Thesis The beer trade trends identifies variables which have an impact on the consumption of beer and predicts the trend of trade in beer in the Czech Republic in the globalization context. To achieve the results was used linear econometric model. Using the Method of least squares determined the size and direction of individual variables for beer consumption. Based on the verified model is a model applicated and based on software Gretl is predicted the beer consumption for a period of 3 years. The biggest influence on beer consumption has production, nominal wages (adjusted for inflation), and the impact of the economic crisis. The prediction is dependent on the lingering economic crisis, beceause the predicted values for 2015 are higher than beer consumption in the same year by 3 litres. The lingering economic crisis has an impact in 2015 on the consumption of beer and during 2016 this dependence should disappear. Trend beer consumption is rising to the level of 146 l per person per year.
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Optimizing of weed control in carrot anf lettuce
Šuk, Jaromír ; Jursík, Miroslav (advisor) ; Kolářová, Michaela (referee)
The main aim of this work was to made literary review about possibilities of weed control in carrot and lettuce. The experimental part of this work was focused on optimization of herbicides weed control in lettuce and carrot. Small plot field trials were carried in 2015.
Literary review was focused on the summarizing of information about the particular vegetables. Futhermore, the available literature about weed control in these vegetables were complied, specialy with a focus on mechanical and chemical (herbicide) control. The last section of review is dedicated to optimizing use of herbicides that are not registered in tested vegetables.
In lettuce, herbicidal efficacy was assessed on Chenopodium album, Amaranthus retroflexus, Mercurialis annua and Echinochloa crus-gali and selectivity of herbicides to crop. In carrot, herbicidal efficacy was assessed on Amaranthus retroflexus, Solanum physalifolium, Chenopodium album, Echinochloa crus-gali and Mercurialis annua and selectivity of herbicides to crop.
Lettuce was treated by herbicide combinations with active ingredients: befor planting or postemergence pendimethalin, postemergence propyzamide, postemergence phenmediphan, postemergence dimethenamid and postemergence pethoxamide. Carrot was treated by herbicide combinations with active ingredients: preemergence aclonifen, preemergence clomazone, preemergence dimethenamid, preemergence pendimethalin, postemergence linuron and postemergence metribuzin.
In lettuce, were on the plots, where were used pendimethalin and dimethenamid. In carrot, best weed control were on the plots, where were used aclonifen, clomazone and dimethenamid. However, after application these herbicides, highest phytotoxicity was found. The lowest selectivity to both tested vegetables showed dimethenamid.
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Change trajectories of wetlands in the landscape of lowlands and uplands in the Czech Republic
Richter, Pavel ; Skaloš, Jan (advisor) ; Dagmar, Dagmar (referee)
Wetland habitats are of great multifunctional importance in landscape. In particular, they have the capacity to retain water during its surplus and then in the dry season to release the water. In this work the dynamics of change in wetlands coverage was analyzed, including the representation structure of different categories of wetlands over the past 180 years in the landscape of lowlands and uplands in the Czech Republic. The historical maps of Stable Cadastre, current orthophoto and current GIS layers of localization and classification of different types of land use/cover and landscape types were used as data sources. The area of wetlands has dramatically decreased from 5 762 hectares in the period 1825-1843 (more than 9.5 % of the area) to 54 hectares in 2014 (0.9 %). Based on the study of available documents three types of wetland habitats were classified: wet meadows, wet meadows with trees and swamps . Although, the most of the historic wetlands were constituted of wet meadows (89 %) these days the swamps (48 %) form the largest part of the wetlands. Half of the historic wetlands were transformed to arable land because of increasing production of agriculture. According to the evaluation of monitored changes by predictors it was found that more pressure on the degradation of wetlands was in the areas with more suitable climatic conditions for agriculture (e.g. The Danube basin, Moravia, soil types in the lowlands, Corn agricultural production area etc.). In these areas the swamps were formed as new wetlands in places of historical watercourses localization more significantly than elsewhere in monitored area. The aim of this work was also to describe the main driving forces and pressures that have influenced the changes in wetlands. The outcome of the paper can be used in landscape planning with regard to the protection and management of wetlands.
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Genetic Diversity of Semi-captive Population of Western Derby Eland (Taurotragus derbianus derbianus) in Senegal and Phylogenetical Relationships between Western Derby Eland (T. d. derbianus) and Eastern Giant Eland (T. d. gigas)
Zemanová, Hana ; Lukešová, Daniela (advisor) ; Jarmila, Jarmila (referee)
Representatives of family Bovidae are subjects of many studies concerning with their phylogeny, phylogeography, time of divergence or genetic diversity. Taxonomy is solved by comparison of morphological characteristics or by genetic approaches, genetic diversity could be solved by pedigree or by genetic analyses too. Tragelaphinae number nine species of two genera, Tragelaphus sp. and Taurotragus sp. The antelopes of the genus Taurotragus (T. derbianus and T. oryx) belong to the largest antelopes of the world. Derby eland (Taurotragus derbianus) has two subspecies, Western Derby eland (T. d. derbianus) and Eastern Giant eland (T. d. gigas), which are distinguished on the basis of morphological characteristics. Western subspecies (T. d. derbianus) is classified as critically endangered. There lives the only population in Niokolo Koba National Park in Senegal, which numbers fewer than 200 individuals. For the conservation, the semi-captive breeding programme has been established in 2000. It was created by six founders (one male and five females), which are presumed to be non-related. The population within this programme had 95 living individuals in 2013, living in seven herds in Bandia and Fathala reserves in Senegal. The population is under breeding management, which efforts to minimize kinship of the individuals. Studbook was established for the Western Derby eland (T. d. derbianus) in 2008 and is published annually. It acts about small population with low number of founders and no gene flow, which is threatened by inbreeding and genetic drift. Genetic diversity of the population was evaluated by means of microsatellite markers and the results were compared with the results of pedigree analysis. Pedigree analysis showed the highest genetic diversity in the generation of founders (FOUNDERS). It decreased in the generation of founders' offspring (OFFSPRING 1; born in season 2007/2008), due to the fact, that the only male took part in the reproduction. And it increased again in the generation of offspring of founders' offspring (OFFSPRING 2; born in the season 2009/2010), because more individuals were included into the reproduction. Fifteen individuals and five polymorphic microsatellite loci (from the total number of 13 tested loci) were chosen for the genetic study. The parameters of genetic diversity (HE and HO, Ar and deviations from Hardy-Weinberg equilibrium, and FIS and FST) were evaluated. Not any deviations from Hardy-Weinberg equilibrium were found out. The results of genetic analysis confirmed the highest genetic diversity in the population of founders (Ar = 2.79; HE = 0.664; HO = 0.750; FIS = --0.154). In both generations of offspring values of allelic richness and observed and expected heterozygosity decreased (Ar = 2.15; HO = 0.580; HE = 0.586 in OFFSPRING 1 and Ar = 2.14; HO = 0.370; HE = 0.480 in OFFSPRING 2). Contrary to the results of pedigree analysis, there was not been observed any improvement in OFFSPRING 2. The resultant values of genetic diversity parameters were quite satisfactory, despite of the low number of founders and mating of related individuals.
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Biotransfer of selected risk metals into plants and their accumulation and distribution in plant organs
Le Minh, Phuong ; Lachman, Jaromír (advisor)
Contamination of soils with heavy metals is one of the serious environmental problems threatening human being. Heavy metals are considered as the special hazard of soil pollutants because of the adverse effects on the plant growth, the amount, activity of useful microorganisms in soils and the quality of food. Regard to the persistent and toxicity, the heavy metals are toxic when we consider different kinds of pollutants in soils. In the soil, zinc (Zn), cadmium (Cd), lead (Pb) and mercury (Hg) toxicities frequently occur than the other metals because of their precipitation and sorption by the soil. It is a very dangerous situation because when these metals are taken up by plants, they can be transported to the food web and food chains.
In the present study, the accumulation of four heavy metals (mercury, zinc, lead and cadmium) in the whole grain of spring accessions of emmer, einkorn and common spring wheat cultivars and potato (Solanum tuberosum) is reported. Heavy and essential elements were monitored in potato cultivars in the exact field experiments and in hydroponically grown plants. The elements were determined by methods FAAS, ET AAS, and AMA (Advance Mercury Analysis). Statistical analyses were performed using SPSS 9.0 with the Tukey HSD (Honestly Significant Difference) test (alpha equal to 0.05).
In our study, the concentration of heavy metals decreased in the order zinc (Zn) > lead (Pb) > cadmium (Cd) > mercury (Hg) in the wheat grain. The comparison between three varieties of investigated wheat revealed that the emmer wheat was rich in zinc content (62.12 mg kg-1 dry matter), while the spring wheat had the lowest average concentration of zinc in the grain (40.99 mg kg-1 dry matter). Generally, the values of lead concentration in grain wheat varieties were low (ranging from 0.1268 mg kg-1 dry matter to 0.2950 mg kg-1 dry matter).
The concentrations of mercury in four typical growth stages of wheat (boot stage 10, heading stage 10.2 1/4 of head emerged, leaf-stage 10.2 and stage ripening 11 according to Feekes) were also determined. It has been shown that the concentrations of mercury in different wheat varieties were absorbed differently at different growth stages of plant. Stage 10.2 and leaf stage 10.2 showed the high mercury content (0.0152 mg kg-1 dry matter and 0.0214 mg kg-1 dry matter, respectively). Among individual varieties significant differences were determined.
Amounts of toxic and potentially toxic elements detected in investigated potato tubers are characterized by a large variability within investigated groups. Performing statistical analysis (one way ANOVA) showed that there were no significant differences between two investigated groups of samples (samples from Uhříněves and Valečov in the year 2013 and 2014) considering either one of investigated metals. Measurable levels of mercury were found in smallest amounts in all investigated potato samples comparing to other metals (Cd, Pb).
Plant cells compared to animal cells are characterized by the formation of cell walls. Plasma membrane or cell membrane is a biological active membrane separating the interior of cell from the outside environment. An adjusted method for isolation of protoplasts was developed and adapted for isolation of protoplasts from plant material (potatoes). In our experiment, the plants were grown hydroponically in the Research Institute of Plant Crops Prague-Ruzyně. If we examine the plant membrane, one option is to remove the cell wall by means of special mixture enzymes. Protoplasts were released in the dark at 25 degrees of Celsius for 18 hours. The 70 and 90 microns sieve was used to filter and then centrifugation for 5 minutes at 100 x g. All the steps were carefully carried out to prevent the damage or breakage of protoplasts.
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Effect of climatic and environmental variables on changes in numbers and migratory behaviour of wintering and migrating waterbirds.
Adam, Matyáš ; Musil, Petr (advisor) ; Bejček, Vladimír (referee)
Waterbirds with their specific habitat and food requirements varying during their annual cycle (Riffell et al. 2003; Taft and Haig 2006) are able to indicate the wetland diversity and quality due to their rapid responses on changes in environment (Delany 1999; Fernández et al. 2005; Amat and Green 2010). Remarkable land cover changes and climate warming led to significant shifts in distribution and abundance of many waterbird species across Europe in recent decades (Delany et al. 2006; Fox et al. 2010; Lehikoinen et al. 2013; Pavón-Jordán et al. 2015). To understand the dynamic of migratory birds in space and time and to assess effects of global conditions as well as local conditions of individual sites during their annual cycle there is need of international monitoring and research. Since the start of International Waterbird Census in 1967 both increasing and decreasing trends have been recorded in nearly fifty percent of waterbird species in Western Palearctic (Delany et al. 2006, Wetlands International 2016) and they consequently have affected trends in particular countries, including the Czech Republic. Wetland sites in the Czech Republic are generally situated on the edge of wintering range of most waterbird species (Gilissen et al. 2002), however the prevailing increase in abundance of waterbird species has been recorded here in recent decades (Musil et al. 2011). Though, the considerable growth of winter temperatures has not been noticed in the Czech Republic (Klein Tank et al. 2002; Musilová et al. 2009; Dušek et al. 2013), and the accessibility of the wetland sites, due to their freezing, varies year to year. Hence, we can assume that waterbirds have likely began using the alternative habitats with available food resources, i.e. cold-weather refuges, probably regardless of their conservation status (Musilová et al. 2015). Special protection areas were implemented to Czech legislation in 2004 to protect migratory birds (Birds Directive 2009/147/EC). So far there has not been tested the effectivity and impact of legislative protection on wintering waterbird species. Moreover, some previous studies indicated that SPA network do not match the species distribution pattern (López-López et al. 2007; Briggs et al. 2012; Albuquerque et al. 2013), so this issue urgently calls for scientific research.
The second part of the thesis focused on Greylag Geese, whose abundance has rapidly grown across the Europe in recent decades (Madsen et al. 1999; Fox et al. 2010), and that have become ideal model species to observe their responses to habitats and climate changes as well as their reactions to human disturbance (Fox and Madsen 1997; Ramo et al. 2015). This requires appropriate knowledge of geese distribution, abundance and their behaviour. Since 1930s, when the geese started to be ringed in the Czech Republic, the ringing intensity have markedly varied and have been reflected in numbers of recoveries. In last ten years the intensity have increased (Podhrazský 2010). However, complex of the historical data until 2002 (Cepák et al. 2008) and recent data have not been analysed so far. In the light of recent shifts in wintering ranges and migration phenology of many goose populations these analyses require increased attention. Furthermore, the satellite monitoring of geese is coming to detect more detailed information about behaviour of individuals.
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Testing of bioeffectors on phosphorus mobility in soils
Holečková, Zlata ; Balík, Jiří (advisor)
Crave for increase in agricultural production over the past sixty years reflects in widespread use of mineral fertilizers. Due to the growth of the world population an increase of overall demand for feed and food is expected. Limited availability of cultivatable soil and increasing dependance on mineral fertilizers can be also anticipated. It is therefore important to find alternative strategies for plant nutrition. In 2012, the project of cooperation between several universities in Europe was revealed. This project examines the use of bioeffectors in crop production. Use of these substances should help to reduce the input of mineral fertilizers used in agriculture and improve land usage. Thesis will perform testing in real conditions of different geographic locations. The main objective is to develop new approaches to the use of so-called bioeffectors based on the mechanism of action of living microorganisms and active natural substances. Bioeffectors may be, depending on soil and climatic conditions, a key factor for overcoming limitations in the availability of nutrients. They can contain microorganisms (bacteria, fungi) and active natural compounds (digest from soil or compost, microbial residues, plant extracts, metabolites from biological processes, dried herb material or seaweed). These substances are developed for a wide variety of crops (e.g. corn, wheat and tomatoes). Their effectiveness consists in nutrients mobilization from less accessible forms in the soil. The thesis is mainly focused on the impact of bioeffectors on various forms of phosphorus in the soil. Phosphorus in the soil belongs to essential elements necessary for the growth and development of plants. Despite its necessity in plant metabolism is its content in the soil relatively low. A higher phosphorus content is in most samples of soil mainly in the surface area because an increased biological activity occurs there causing the accumulation of organic material. Some influence can be also observed in connection with application of organic and mineral fertilizers. Amount of phosphorus in soil may vary depending on parental rock type, texture and land management (the ratio of P supplied and type and method of land cultivation). Above mentioned influences even have an effect on relative amount of organic and inorganic forms of phosphorus. Three basic groups of phosphorus contained in the soil can be described: inorganic phosphorus compounds, organically bound phosphorus and exchangeable absorbed phosphorus. The amount of phosphorus bound in the fractions depends mainly on the time of fertilizer application, including the impact of earlier interventions. For these reasons, it is necessary to execute detailed analysis of the various forms of phosphorus in the soil after application of mineral fertilizers together with bioeffectors. In this dissertation variety of pot and field experiments will be set up, where samples from each variation of soil and plant will be separated and researched. Samples will be analyzed and amounts of phosphorus will be measured.
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