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Analysis of sheep utility attributes in ecological and conventional flocks in the Czech Republic
Linhartová, Iveta ; Ptáček, Martin (advisor) ; Jan, Jan (referee)
The goal of this diploma thesis was to perform an analysis of sheep breeding of Suffolk breed in basic reproductive performance and meat production traits aspects. The information for rating these aspects was used from two farms. The first of them used conventional way of breeding (ZVOZD Horácko, družstvo) and the second one used ecological way (EkofarmaKosařůvmlýn, s.r.o.).
Fertilization, fertility, intensity and rearing belong among monitored reproductive indicators. These reproductive indicators are expressed in percentages. Regarding meat production traits rating in this thesis we compared influences of year of lambing, farming method, birth month, sex, litter size and age of dam. All these influences were judge according to birth weight (kg), weight at the age of 100 days (kg), average growth (g), depth of the longest dorsal muscle (mm) and according to depth of subcutaneous fat (mm). Reproductive performance and meat production were monitored in years 2011, 2012, 2013, 2014 and 2015.
In terms of reproductive performance with a few exceptions it was true that ZVOZD Horácko, družstvo reached the best values, and in most cases better than the national average. For example in terms of intensity values in 2012 ZVOZD Horácko, družstvo had 35,9% better results compared to national average. In the same year this farm reached 48,1% higher values of percentage rearing compared to national average. Ekofarma Kosařův mlýn, s.r.o. showed worse performances, however it was in most cases better than national average. In terms of intensity in 2011 Ekofarma Kosařův mlýn, s.r.o. had the same results as the ZVOZD Horácko, družstvo. Meat production properties were supported by statistical calculations and all of them were conclusive at least at the level of significance P < 0,05 in all cases except month of birth effect on birth weight. The best results of meat production in terms of effect of lambing had the year 2012, when the birth weight was one of the highest and the other properties (weight at the age of 100 days, average growth, depth of the longest dorsal muscle, depth of subcutaneous fat) were by far the highest in the period of 2011-2015. Next we found out that the ecological way of breeding (in this case Ekofarma Kosařůvmlýn, s.r.o.) has better results than the conventional way (ZVOZD Horácko, družstvo).
The effect of birthmonth has the significant influence on the indicators of meat production and the lambs born in April had the best results. In the case of sex rams are better. Litter size has very important influence on meat production the more lambs per one mother, the lower
birth weights, the lower weights until the period of weaning, the lower weights at the age of 100 days. It is affected by milkiness of the mother. Moreover, the age of mothers also play a significant role. In our research the highest birth weights had lambs of one year old mothers and all other indicators were the highest in the case of three year old dams.
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Realization of Carcass Poultry on the Market
Polláková, Simona ; Kovářová, Kateřina (advisor)
Goal of the thesis
The main objective of this thesis is to evaluate current demands on commercialization of carcass poultry on domestic market. This goal of the thesis will be divided into several following parts:
1. Defining quality of poultry meat from the perspective of physical attributes, chemical composition and elements that influence them,
2. characterization of the standards in nutritioning carcass poultry and quality criteria, which are deciding factors for commercialization on the market,
3. introduction to trade and criteria for purchasing carcass poultry on domestic market.
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Using of the Czech Warmblood for the Czech Republic Mounted Police
Vlčková, Petra ; Navrátil, Jan (advisor) ; Karel, Karel (referee)
The horse evolution started with Hyracotherium, which was a small animal inhabiting forests of the Eocene. Since then, it evolved into more or less successful forms in which the conditions of the surroundings of its occurrence were reflected. When it comes to the initial usage of horses, they were used as a source of meat. Later, in the period before 4,600 years ago, evidence of the domestication of these animals emerged. Due to the improved system of breeding, horse as a draught animal and a mean of transport gradually became riding horse, which contributed not only in spreading human population, but also in influencing history of many civilizations.
Nowadays, horse is represented through a number of breeds, which are used in equestrian sport, work with cattle, horse races, in forestry labours, or as a good companion.
In addition, horse gained its irreplaceable role in the service of the Mounted Police of Czech Republic where it is daily deployed in diverse actions; for example in city and peripheral guarding, in searching for missing persons or in restoration of disturbed public peace. The most useful breed for these purposes is Czech Warmblood, where the most preferred geldings are in brown or chestnut color. High demands are placed on physiological characteristics; however, the most important criterion is the excellent character of this breed. Quality of these characteristics consists in the length of duty and in the reasons for their release from duty. Some of the most frequent reasons for the release from duty health problems and age. The release due to age is associated with strong and resistant horses.
Regarding the lineage of the police horses on duty, the best evaluated were the descendants of stallions 1154 Quick Lauro Z, Mykonos and 1028 Manillon Rouge. However, there is a correlation between these descendants and their number on active duty. After categorization of all traced stallions into relevant lineages, the lineage 1100 Przedswit became the most numerous lineages (7 stallions). Nevertheless, this lineage is not positively evaluated because of its descendants, which did not perform well on duty. Another numerous lineages were 5500 Pythagoras, 4700 Amour du Bois, Orange Peel xx-Almé Z, 67 Dark Ronald or 4800 Ladykiller XX.
The central purchase system of duty horses should help to unify these horses as well as to prolong the length of duty, via universally established criteria. Results demonstrate the unification of typological characteristics of these horses. However, the average withers height of measured horses fails to meet the requirements. Regarding the length of duty, the results were not conclusive; therefore, the hypothesis was confirmed just partially.
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An impact of organic farming on soil quality
Matěchová, Martina ; Jakšík, Ondřej (advisor) ; Janků, Jaroslava (referee)
This work focuses on the system of organic agriculture and its impact on soil quality. It compares organic farming system with conventional. Both these systems are briefly described. There is also a list of soil quality indicators used for the soil quality is evaluation. The results of scientific studies, which dealt with different farming systems and their effects on soil properties, were discussed and evaluated.
Quality and fertile soil is crucial for production systems, however to define what means quality soil is very difficult. Therefore the number of quality indicators were established. The indicators can be grouped by their nature of soil properties they assess, such as physical, chemical and biological. Different techniques are adopted for soil quality evaluation.
Soil quality is affected by a lot of different factors. Anthropogenic activities are often considered as one of the most influential factor. The loss of soil fertility as well as other physical, chemical or biological deterioration of soil properties is called soil degradation.
The results of this study shown that careful tillage in organic farming could have positive effect or no effect on the biological and physical properties of soils. For some experiments there was an increased amount of soil organisms.
In the most experiments, application of organic fertilizers caused increase of organic matter and other essential nutrients. We conclude that under certain conditions with respect for the principles of organic farming the soil quality can be preserved or even improved, but in a longer timeframe.
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Traditional Methods of Fish Preservation in Southeast Asia
Holmanová, Kateřina ; Banout, Jan (advisor) ; Leuner, Olga (referee)
Traditional methods of fish preservation are still often used in developing countries, which produce significant amount of fresh fish. The most frequently used methods of processing fish in Cambodia are drying, salting, fermentation, smoking and marinating. Sun drying as one of the most common methods of fish processing in Cambodia has some disadvantages, which increase spoilage of final product. Salting contributes to increase efficiency of drying process. Salt creates highly salty environment, which the most bacteria, fungi and other potentially pathogenic organism cannot survive in. The aim of this Bachelors thesis was to carry out research focused on drying method in combination with salting. For five selected spices of freshwater fish: Claris batrachus, Channa micropeltes, Oreochromis niloticus, Pangasius hypophthalmus and Monopterus albus were determined dry matter content, fat content and salting effect on the amount of salt. Samples, before drying in direct solar dryer with natural convection and electric oven, were salted for 2, 4 and 12 hours. Results of measuring of dry matter content are within the interval 16.67 to 21.74 g on 100 g of fresh muscle, the lowest values were measured at C. batrachus and the highest at O. niloticus. Measuring of fat content showed difference in the interval 2.29 - 30.08 %, the lowest value at M. albus and highest at P. hypophthalmus. From the measurements of amount of salt were found influence of the salting time on the total amount of salt. Values increased with the higher time of salting. An important prerequisite for achieving high-quality dried products is characteristic properties for individual fish species to achieve maximum yield.
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Factors affecting the properties of glued joints for glues used in the wood industry
Boška, Pavel ; Böhm, Martin (advisor)
Dissertation focuses on the influence of selected factors on the properties of the bond glued by acetate polyvinyl adhesives. Factors such temperature and moisture content of the environment, the pressing pressure and cyclic stress. Despite the same type of adhesive, the properties may vary for different producers of adhesives and according to which the product is not intended. Influence will be compared to the test samples of dried beech timber with regard to shear bond strength. They will be compared properties of adhesives from different manufacturers and different destination. Test samples and test progress carried out in accordance with current standards, or largely based on. This research should help users streamline the process of adhesive bonding and reduce the production costs of the bond.
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Suitable Methods of Determination and Varification Ammonia and Selected Greenhouse Gasses Detection
Zabloudilová, Petra ; Pecen, Josef (advisor) ; Jan, Jan (referee)
The dissertation deals with methods of detection gases from agriculture, especially ammonia. To understand and control of ammonia and others gases (i.g. methane) depends on sampling and measurement techniques that includes devices, instruments and procedures. In the theoretical part are listed the basic characteristics of ammonia and selected greenhouse gases and its environmental effects, sources of emissions and characteristics of chosen gas sensors (especially semiconductor sensors). In the experimental part of the work were tested conditions of chosen types of sensors (especially semiconductor sensors) mentioned above in the laboratory, according to the change of relative humidity and concentration detection gas especially. The chosen sensors were verified for measuring in the operational conditions (in the barns). Finally, the advantages and disadvantages of tested sensors are discussed (with regard to use these sensors in operational conditions).
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Mophological and molecular diversity of a tropical tree species Guazuma crinita in the Peruvian Amazon
Tuisima Coral, Lady Laura ; Lojka, Bohdan (advisor) ; Helena, Helena (referee)
Fast growing tree species Guazuma crinita (Malvaceae) was selected as a priority species for domestication in the Peruvian Amazon due its important contribution to the livelihood of local farmers. Its domestication process is still in an early age as for many tropical tree species little is known about its genetic variability and we dont know anything about the impact of domestication on its genetic resources. The main objective of this research was to assess the genetic variability of G. crinita within and among populations in the Peruvian Amazon by the use of morphological (wood physical traits) and molecular (ISSR and AFLP) markers.
Wood physical properties among six G. crinita provenances were evaluated. Wood samples were drilled from the base middle and top of the stem of 12 randomly selected eight-years-old trees for determination of wood measurement. Pearson correlations between physical properties were also determined. All wood physical properties except green density differed significantly among provenances. We also found statistically significant variation due to stem level position. The moderately dense wood and the coefficient of anisotropy (1.6) suggested that G. crinita has stable wood; they represent important advantages in terms of costs for transport and transformation process. The results suggested potential to select provenances with desirable wood properties for further breeding and domestication. Due to the variation found even in limited tree samples it is recommended further analysis with more extensive number of samples from different provenances and planting zones.
This research presents the first assessment of genetic variability based on inter-simple sequence repeat (ISSR) markers for 44 G. crinita genotypes from a clonal garden multiplication established in the Peruvian Amazon Research Institute (IIAP) in Ucayali region. Ten ISSR primers amplified a total of 65 bands of which 61 were polymorphic (93.8%). The range of DNA amplification varied from 260 to 2200 bp. Among the provenances overall genetic differentiation (Gst) was 0.03 indicating 97% of genetic diversity within provenances. Gene flow (Nm) was 12.9 alleles per generation. Cluster analysis was not related with geographic origin suggesting a common gene pool which was supported by calculation of weak positive correlation was found between genetic and geographic distance.
With the use of AFLP markers an insight on how domestication process does impact G. crinita genetic resources is also reported on this research work. I was able to generate fingerprint for 58 leaf samples representing eight provenances and three population types, 19 from a natural regenerated population 15 cultivated in home garden nursery and 24 from a collection of genotypes considered as semi-domesticated population. Seven selective AFLP primer combinations were used. A total of 171 fragments were amplified with 99.42% of polymorphism at species level. Each type of population generated fragments with 72.51% 49.12% and 54.39% of polymorphic fragments respectively. Neis genetic diversity and Shannon index information were found to be higher in the population of natural regeneration compared to overall semi-domesticated population (He = 0.10 and 0.9; I = 0.19 and 0.16 respectively). The analysis of molecular variation (AMOVA) showed higher variation within provenances rather than among (84% and 4% respectively). UPGMA clusters analysis and PCoA did not showed correspondence between genetic and geographic distance in addition their correlation was not significant. There was a significant genetic differentiation among types of population suggesting slight genetic bottleneck in semi-domesticated populations yet with relatively high levels of genetic variation.
In situ conservation for populations with high levels of genetic diversity was recommended. In addition proper management of natural regeneration and ex situ genotype collections might be a good conservation strategy to maintain G. crinita genetic resources.
The use of morphological (wood physical traits) and molecular markers were successful to reveal genetic variability of G. crinita and they could be used for other tropical tree species. For further researches it is emphasis to extent the number of samples and geographic scale.
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Assessment of in vitro antioxidant properties of medicinal and edible plant extracts
Tauchen, Jan ; Kokoška, Ladislav (advisor) ; Jaromír , Jaromír (referee)
Identification and characterization of plant-based products with antioxidant and anti-proliferative effects has received much interest over the past few years as possible therapeutic mean for treatment of diseases likely to be associated to oxidative stress (such as atherosclerosis, diabetes, and cancer). This study provides characterization of in vitro antioxidant and/or anti-proliferative potential and phytochemical profile of (i) 39 wine samples of underutilized Georgian grapevine cultivars, (ii) extracts of 22 samples of medicinal plants from Ethiopia and (iii) 23 samples of edible and medicinal plants from Peruvian Amazon. For this purpose, 2,2-diphenyl-1-picrylhydrazyl radical assay (DPPH), oxygen radical absorbance capacity (ORAC) assay, total phenolic content (TPC), and cell viability assay based on metabolization of tetrazolium bromide (MTT) to formazan, together with methods based on high performance liquid chromatography (HPLC-ultra violet/visible spectrometry and HPLC-tandem mass spectrometry) were used. Georgian red wines (particularly Saperavi cultivars) exhibited higher antioxidant capacity (average DPPH and ORAC values at 5.1 and = 10.6 g TE/L wine, respectively) in comparison to Central and West European cultivars [Pinot Noir (DPPH = 3.1 and ORAC = 9.4 g TE/L wine), Cabernet Sauvignon (DPPH = 3.0 and ORAC = 7.3 g TE/L wine) and Cabernet Moravia (DPPH = 2.0 and ORAC = 8.5 g TE/L wine)]. Georgian wines contained significantly greater concentrations of quercetin (between 14.44 and 1.07 ug/mL), kaempferol (between 1.68 and 0.03 ug/mL) and syringic acid (between 12.59 and 4.72 ug/mL), whereas possessed lower quantities of resveratrol (between 5.11 and 0.32 ug/mL) in comparison to Central and West European wines. Amongst edible and medicinal plants from Ethiopia and Peruvian Amazon, only Dodonaea angustifolia (IC50 for DPPH = 22.2 ug/mL, ORAC = 767.6 ug TE/mg extract; IC50 for Hep-G2 = 120.0 ug/mL), Rumex nepalensis (IC50 for DPPH = 5.7 ug/mL, ORAC = 1061.4 ug TE/mg extract; IC50 for Hep-G2 = 50.5 ug/mL), Inga edulis (DPPH and ORAC = 337.0 and 795.7 ug TE/mg extract; IC50 for Hep-G2 and HT-29 = 36.3 and 57.9 ug/mL) and Oenocarpus bataua (DPPH and ORAC = 903.8 and 1024.4 ug TE/mg extract; IC50 for Hep-G2 and HT-29 = 102.6 and 38.8 ug/mL) have demonstrated combinatory antioxidant/anti-proliferative efficacy. Selective anti-proliferative activity was observed for Verbascum sinaiticum (IC50 for Hep-G2 = 80.6 ug/mL) and Annona montana (IC50 for Hep-G2 and HT-29 = 2.7 and 9.0 ug/mL, respectively). Above-mentioned plant material showed only weak or non-toxic effects towards normal cell line. Despite the fact that extracts of Jasminum abyssinicum (IC50 for DPPH = 26.3 ug/mL, ORAC = 1023.7 ug TE/mg extract), Rumex nepalensis (IC50 for DPPH = 5.7 ug/mL, ORAC = 1061.4 ug TE/mg extract), Mauritia flexuosa (DPPH and ORAC = 1062.9 and 645.9 ug TE/mg extract), Myrciaria dubia (DPPH and ORAC = 641.9 and 642.6 ug TE/mg extract) and Theobroma grandiflorum (DPPH and ORAC = 714.8 and 821.9 ug TE/mg extract) have exhibited considerable antioxidant effect, these species were found to possess moderate to low anti-proliferative potential or have shown to be toxic to normal cells line. In all cases it was detected that phenolic compounds content correlated strongly with antioxidant activity, however weakly with anti-proliferative effect. Results suggest above-mentioned species as prospective materials for further development of novel plant-based agents effective against oxidative stress related diseases. However, it is necessary to perform further research which would be focused on detailed characterization of their chemical composition, pharmacological effects and toxicological safety, in order to verify their possible practical use.
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Effect of Drying Pretreatments on Air and Solar Drying of Jerky Prepared from Eland (Taurotragus oryx) Meat
Kučerová, Iva ; Banout, Jan (advisor) ; Oliver, Oliver (referee)
Mathematical modeling of thin-layer solar drying and organoleptic properties of eland jerky was investigated in this study. Eland jerky was compared to the traditional beef jerky, inasmuch as both were treated with traditional jerky marinade (TM), TM with fresh pine apple juice (TMP), TM with honey (TMH), TM with Coca Cola (TMCCL) and compared to an untreated control (C). The influence of the marinades on the drying process was statistically significant. Based on the coefficient of determination, the root mean square errors and the chi-squares, the Two-term model was found to be the most suitable model for describing the solar drying kinetics of eland jerky. The mean effective moisture diffusivities of solar dried eland meat for the C and selected pre-treatments TM and TMH samples were 2.07 x 10-10, 1.45 x 10-10 and 1.43 x 10-10 m2.s-1, respectively. The activation energy values for solar dried eland jerky were 23.75, 26.22 and 26.97 kJ.mol-1 for C, TM and TMH, respectively. Organoleptic properties of dried eland meat was assessed by the 22 member degustation panel. The best scored pre-treatment was TMP, which has significant effect on texture, color and taste. Effect of the different pre-treatments on the overall combined color was calculated. Generally for both meat dried in both driers TMH marinade was evaluated as the one with the highest total difference contrariwise meat dipped in TMP pre-treatment has the lowest total difference.
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