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Seed germination and seedling recruitment of Iris sibirica depending on the type of management
HOŘEJŠÍ, Edita
This work continue on a bachelor's work which investigated production characteristics of the plant species Siberian Iris (Iris sibirica). The aims of the presented work are reproductive properties by seeds of Siberian Iris in relation to the seed germination and with various meadow managements. Seeds of Siberian Iris (collected in 2015 and 2016) were investigated in laboratory condition and at meadow near the pond Dolní Stržený (Dlouhá Lhota, of the Tábor District). Seeds were divided into groups according to 4 treatments: without intervention, scarification, thermal stratification and cold stratification. The field experiment runs from September 2016 to August 2017.The aim was to find out the most appropriate type of management (mowing, disturbance) that would promote seed germination and seedling recruitment. Results from the both experiments: a positive effect of germination after the scarification, better recruitment of seeds in square without intervention, seeds probably needs to be stored for a longer period before sowing. These results cannot be generalized, because small number of repetitions were performed. All the obtained data was discussed and recommendations for the plant management were formulated.
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Changes in timing of germination caused by neighbouring seeds and how it is connected with species traits.
Kos, Pavel ; Weiser, Martin (advisor) ; Hadincová, Věroslava (referee)
The time when the seed germinates is very important. Ability to change the time of germination may be very advantageous. It allows the emerging seed to choose the best time according to abiotic conditions, and also to avoid of competition with neighbouring individuals. The seed reacts not only on adult plants and seedlings, but also on other seeds, with which is able to communicate. For a better understanding to this mechanism I conducted an experimental study with twenty-six species. The species were selected according to their position in long succession seres of mesic/xeric abandoned fields in Český kras. The seeds were left to germinate in pairs in all combinations among them. Here I present the results where I show how the time of emergence changes, depending on presence of neighbouring seed. Also, I show how these changes in germination are related to species specific traits. Out of this, I tried to influence communication between seeds by adding activated carbon. Activated carbon should stop the communication by highly absorbing surface. The time of germination of seeds which germinated alone was not proportional to the time of germination of seeds which germinated with neighbours. This correlation showed up like nonlinear, late-emerging seeds emerging unproportionally later when emerging...
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Seed germination of selected crops after low-temperature treatment
PETRÁŠKOVÁ, Kamila
The influence of low-temperature plasma on the different seeds is currently one of the most used techniques in agriculture. The aim of my bachelor thesis is to monitor and evaluate the effect of low-temperature plasma on the seeds of maize sow (Zea mays L.) and oilseed rape (Brassica napus L. napus), particularly on the germination of the seeds, germination rate and germination energy. These characteristics were measured by germination tests and they are expressed as a percentage. The submitted thesis informs whether the stimulation of the seeds by plasma affects these plants and if so, whether the influence is positive or negative.
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Effect of climate variation on species germination
Duongová, Thuy Lien ; Münzbergová, Zuzana (advisor) ; Kladivová, Anna (referee)
Climatic changes have been influencing plants since their colonization of land. Plants always find a way to adapt to these new changes. Also germination of seeds and seedling recruitment have been adapting to climate change. During the last 150 years the current global warming is changing the germinalibity of seeds, the timing of germination, the rate of germination and the seedling recruitment. This bachelor thesis summarizes results of studies on how climate change effects the germination of plants and the seedling recruitment. This work also presents methods used for studying these questions. Studies often focus on germinalibity, the rate of germination and the timing of germination. Some of the species are profiting from these changes and we can observe faster germination and better germinalibity. Reduction of germination occurs in other species and so their fitness is also reduced. The number of studies observing inter-specific variability in germination is greater than studies on intra-specific variability. In my future work I would thus like to focus on the effect of climate change on intra-specific variability in germination. Powered by TCPDF (www.tcpdf.org)
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Germination requirements of rare and common plant species of Giant Mountains
Paulů, Andrea ; Münzbergová, Zuzana (advisor) ; Moravcová, Lenka (referee)
For a long time studies were focused on searching optimal conditions of germination of individual species. During the last few years studies have started to search for a relationship between germination and various species characteristics. Most of them, however, use only one temperature during germination tests. Very few studies use more than one temperature during the germination tests. In the last years studies started also to compare germination between rare and common species, but these studies compare just a few species. There are no studies which would compare germination on broader range of species. Aim of this study was to indentify which factors are determing germination of species and to find relationships between germination and characteristics of rare species (n=62). Another aim was to find out differences in germination of pairs (n=24) of closely related rare and common species. To test the germination I have chosen a methodology with several consecutive temperatures. Throughout the time of testing , the seeds were placed either in light or in dark. The dataset was processed by linear regression. The results show that germination requirements of species are determinet by time of flowering, weight of seeds, dispersal mode, species requirements for soil humidity and nutrients and type of...
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Ecophysiological relevance of extreme sensitivity of orchids to nitrates
Figura, Tomáš ; Ponert, Jan (advisor) ; Tylová, Edita (referee)
Many orchid species are seriously endangered at present. Reasons for their disappearing from natural habitats remain often unclear. Orchids depend on mycorrhizal symbiosis in nature, however only little is known about this symbiosis. Seeds of some species do not germinate in vitro, making their cultivation for scientific and rescue purposes impossible. We found that seed germination of one of such reluctant species, Pseudorchis albida, is strongly inhibited by nitrates even at extremely low concentrations. As this species prefers oligotrophic mountain meadows, nitrate-induced inhibition probably take place in natural conditions. Surprisingly, we found similar but slightly weaker inhibitory effect also in typical mesophillic species and even in slightly eutrophic ones. The sensitivity to nitrates correlates with trophy level of species canopy. This inhibitory effect of nitrates could be weakened by application of a range of growth regulators, including auxins, cytokinins and gibberellins, and also by mycorrhizal fungi. The action of nitratereductase is essential for this inhibitory effect of nitrates. Experiments with NO donors, scavengers, and NO quantification are pointing right at NO as compound which perhaps mediates nitrate inhibitory effect. Based on these results, the nitrate inhibitory...
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The effect of temperature on plant growth: teaching protocols of model experiments
Máhrlová, Zuzana ; Albrechtová, Jana (advisor) ; Mourek, Jan (referee)
This diploma thesis is focused on the creation of model tasks (by means of an experiment) for the study of biology at secondary schools and concerns the effects of temperature on plant growth. The selected tasks utilize the new didactic approach by means of the so called inquiry based education. This method of teaching contributes to the development of individual deduction reasoning and encourages the students to actively think about the subject matter. The thesis provides the necessary theoretical background for the topic together with the description of methods and material needed for the conducting the experiments. Furthermore, the thesis describes the necessary instructions and procedures to conduct the experiment and its use in the secondary school teaching practice and presents the requisite methodical guidelines and worksheets for them. Simultaneously, the thesis verifies the didactic applicability of the experiment and the worked out materials through a questionnaire survey and monitored application of the model tasks at secondary schools. The main result of this diploma thesis is a modern, functional and verified method of teaching for high school about the effects of temperature on plant growth in accordance with modern approaches to education, which include own experiments, their...
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Identification and modeling of gene expression regulatory networks during streptomycetes germination
Straková, Eva ; Vohradský, Jiří (advisor) ; Stopka, Pavel (referee) ; Vondrášek, Jiří (referee)
Streptomycetes have been studied mostly as producers of antibiotics and for fundamentals of complex bacterial cell development. Here, transcriptomic and proteomic approaches were applied to systems study of Streptomyces coelicolor germination as a developmental transition from dormancy to the vegetative stage. The time dynamics of the gene expression levels represented by mRNA and intracellular protein accumulation and synthesis were measured throughout 5.5 h of germination at 13 time points by employing both DNA microarray and two-dimensional gel electrophoresis techniques. Using a numerical model of gene expression, genetic networks were reconstructed and functional groups of genes controlled by the sigma factors were identified. Modeling of the regulatory interactions provided a set of parameters allowing simulate kinetics of gene expression control among the sigma factors and their target genes. Particularly regulons of two sigma factors, SigR and HrdD, were identified. The analysis assigned their key role during the germination process. Analysis of global trends in the gene/protein expression revealed that the full capability of regulatory mechanisms responding to the environmental cues is reached within the first hour of germination, and identified the basic gene/protein functional groups...
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Seed dormancy mechanisms of mycoheterotrophic plants
Figura, Tomáš ; Ponert, Jan (advisor) ; Weiser, Martin (referee)
Seeds of mycoheterotrophic plants known as dust seeds which are typical for the family Orchidaceae and 11 other families have inner (physiological, morphological) and outer (physical) dormancy. Dormancy breaking it is a necessary step for a seed to germinate. For breaking the exogenous dormancy it is necessary to break both inner and outer testa, which can be done by scarification. Chemical scarification, mostly done by chlorine, is commonly used and it seems to be the best way for breaking dormancy and also for sterilization of seeds. Other sterilization agents as ethanol and sulfuric acid are also commonly used. For breaking the inner (physical) dormancy, application of growth regulators can be useful, especially in the case of cytokinins, specifically kinetine. Abscisic acid induces dormancy and ethylene induces germination. Anorganic forms of nitrogen have also inhibitory effects for germination at least for some species. Seeds of most orchid species also need a period of chilling after sowing and for germination they mostly need temperature around 23řC.
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