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Posouzení procesu delaminace lepených materiálů
Matušinec, Luděk
The thesis aims to explore the process of delamination on glued prism. It will be evaluated different types of wood (SM, DB) in combination with heat-treated wood (Thermowood TW). These materials will be exposed in an environment with high humidity. It will also be assessed shear strength test specimens, which will also be exposed to conditions of environment high humidity. Based on the measurement results will be evaluated different types of glue joints. They will vary in type and quantity of glue. The work is consisting of two parts. The first part deals with literature review on glueing of wood, thermal modification of wood and modern glued materials. The second part of the thesis is practical, which will be determined by the methodology of experiment and the subsequent testing. In this thesis will be displayed charts and graphs that will be evaluated at the end of work and commented for practical use in the company.
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Diagnostics of Hypercoagulation Status by D-dimer Concentration Measurement
STIEBLEROVÁ, Romana
In common thrombofilia is the state of the vessel system in arterial, venous or microcirculation part when the probability of thrombs-creation is increased, but thrombs are not present yet. It is the pre-state of thrombotization. Thrombofilia is denoted as hypercoagulation status. Thrombofilia also is a common term for hereditary and acquired prothrombotic states in arterial, venous and microcirculation system. Progress in thrombotic and anticoagulation treatment and high-powered clinical research in pathogenesis together enable the radical progress in modern conception of pathophysiology in clinical diagnostic and introduce usage of very efficient treatments. Results of big multi-central studies show clearly how the precise fibrinolytic and coagulation tests and treatments can help in long-term surviving of patients with thrombosis. The search part of this study shows a summary of last information in pathophysiology and last potentialities in laboratory tests and interpretations focused on clinical exposure and diagnostic of PE and DVT. Inspiration for this study are results published in JOURNAL OF THROMBOSIS AND HAEMOSTASIS 10 (7): Penaloza, A., Roy, P. -M., Kline, J., Verschuren, F., Le Gal, G., Quentin-Georget, S. , Delvau, N. , Thys, F. Performance of age-adjusted D-dimer cut-off to rule out pulmonary embolism. 1291-1296, 2012. doi:10.1111/j.1538-7836.2012.04769.x and HAEMATOLOGICA-THE HEMATOLOGY JOURNAL 97(10) Douma, Renee A.; Tan, Melanie; Schutgens, Roger E. G.; et al. Using an age-dependent D-dimer cut-off value increases the number of older patients in whom deep vein thrombosis can be safely excluded. 1507-1513, 201, doi:10.3324/haematol.2011.060657. In this part also the need of Good Laboratory Praxis for pre-analytics extraction and transportation of whole blood samples for coagulation and fibrinolytic tests is highlighted. The practical part of this study covers either the laboratory results aimed to confirm the diagnosis or the results of D-dimer concentration done for preventive reasons and selected in specific files. D dimer is the specific fissile product of fibrin and the presence of D-dimer in plasma testifies the activation of coagulation and fibrinolysis. D-dimer tests were done on the automatic coagulation analyzer ACT Elite Pro. D-dimer test has been done by immunological method based on reaction between antigen and antibody. Analyzer ACL Elite PRO uses nephelometry for detection of blood clogs and reads the intensity of scattered 90° angle light in the sample. Results of sonography are from data stored in hospital information system FONS Akord STAPRO Hospital Jindřichův Hradec a.s. All data were collected in several data-files. This study verificates the hypothesis of predicted difference of D-dimers in patients from preventive file and patients in file with thrombofilia diagnosis. The hypothesis of D-dimer test positivity in venous thrombosis was confirmed as well, D-dimer as a marker of thrombofilia shows an actual activation in-vivo system and increased D-dimer concentration confirmed venous thrombosis DVT together with a positive sonography. The most interesting and the most important result of this study is the confirmation of the hypothesis of using an age-dependent D-dimer cut-off in patients over 50 of age with suspect DVT or/and PE diagnosis. The importance of using an age-dependent D-dimer cut-off was published in articles in JOURNAL OF THROMBOSIS AND HAEMOSTASIS 10 (7): Penaloza, A., Roy, P. -M., Kline, J., Verschuren, F., Le Gal, G., Quentin-Georget, S. , Delvau, N. , Thys, F. Performance of age-adjusted D-dimer cut-off to rule out pulmonary embolism. 1291-1296, 2012. doi:10.1111/j.1538-7836.2012.04769.x and HAEMATOLOGICA-THE HEMATOLOGY JOURNAL 97(10) Douma, Renee A.; Tan, Melanie; Schutgens, Roger E. G.; et al. Using an age-dependent D-dimer cut-off value increases the number of older patients in whom deep vein thrombosis can be safely excluded.
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Cyber Bullying in the world of new media
LEPIČOVÁ, Zuzana
The diploma thesis titled Cyber Bullying in the world of new media deals with one of the potential risks associated with the use of new information and communication media cyber bullying. The goal of the thesis is to map the awareness of parents about possible forms of cyber bullying and about the risks associated with the use of modern information technology by their children. The introduction of the theoretical part of the thesis deals with the explanation of basic terms which are associated with the issue of new media, such as: media, new media and cyberspace. Then there is a section which is dedicated to the issue of cyber bullying and its comparison with the traditional bullying. The section deals with forms of cyber bullying and with perpetrators of cyber bullying, and with the forms of prevention, intervention and possible impacts of cyber bullying. The practical part of the thesis contains the description of the used methodology and the description of the research sample. It also includes the results of research and the discussion, which is focused on comparing the results of the research with opinions of experts working in the field of cyber bullying. The strategy of quantitative research, through the questionnaire survey of parents of children and adolescents, was used to verify the hypotheses. The field collection of data was carried out from May 2014 to December 2014. The results of the research show that the parents of children and adolescents are aware of the risks associated with using the modern information technologies. The research proved that the parents know about the forms of cyber bullying as well as about the risks associated with cyber bullying and that they have awareness of what materials are published on social networks by their children. Results of the thesis can be offered to the managements of elementary schools in Písek District. The thesis can be used as an informative material by parents of children and adolescents, and by teachers and educational counsellors at primary and secondary schools.
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Development and optimization of sample preparation step for fast chromatographic analysis
Pilařová, Veronika ; Nováková, Lucie (advisor) ; Bednář, Petr (referee) ; Šatínský, Dalibor (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate Mgr. Veronika Pilařová Supervisor Assoc. Prof. Lucie Nováková, Ph.D. Title of Doctoral Thesis Development and optimization of sample preparation step for fast chromatographic analysis. The aim of the dissertation thesis is the development of modern extraction methods for the sample purification and for the analytes isolation and preconcentration from various biological materials, including biological fluids (plasma, serum, vaginal lavages) and plant material. Modern approaches have a lots of advantages such as a lower consumption of sample and organic solvents, extraction speed-up, better sample purification, matrix removal and lower influence of matrix effects. Extracts from modern microextraction methods can be analyzed by separation methods including ultra-high performance liquid chromatography and supercritical flluid chromatography coupled with mass spectrometry. The separation methods enable fast high-throughput analysis with sensitive and selective detection. Individual projects show the possibilty of use of the modern approaches in the sample preparation field, where the microextraction by packed sorbent (MEPS), microextraction by solid phase extraction in pipette tips...
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High-Performance Analytics (HPA)
Soukup, Petr ; Pour, Jan (advisor) ; Novotný, Ota (referee)
The aim of the thesis on the topic of High-Performance Analytics is to gain a structured overview of solutions of high performance methods for data analysis. The thesis introduction concerns with definitions of primary and secondary data analysis, and with the primary systems which are not appropriate for analytical data analysis. The usage of mobile devices, modern information technologies and other factors caused a rapid change of the character of data. The major part of this thesis is devoted particularly to the historical turn in the new approaches towards analytical data analysis, which was caused by Big Data, a very frequent term these days. Towards the end of the thesis there are discussed the system sources which greatly participate in the new approaches to the analytical data analysis as well as in the technological solutions of High Performance Analytics themselves. The second, practical part of the thesis is aimed at a comparison of the performance in conventional methods for data analysis and in one of the high performance methods of High Performance Analytics (more precisely, with In-Memory Analytics). Comparison of individual solutions is performed in identical environment of High Performance Analytics server. The methods are applied to a certain sample whose volume is increased after every round of executed measurement. The conclusion evaluates the tests results and discusses the possibility of usage of the individual High Performance Analytics methods.
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Quantitative analysis by X-Ray powder diffraction
Stachová, Sandra ; Havlíček, David (advisor) ; Vojtíšek, Pavel (referee)
Quantitative analysis methods using X-ray powder diffraction are still highly actual conception in the area of research of modern materials. The Reference Intensity Ratio (RIR) method is used here for quantitative analysis by X-ray powder diffraction of model samples in this bachelor thesis. The model samples are three mixtures of corundum, mullite and calcite. To get diffraction patterns of the mixtures, two different diffractometers are used and their outputs are then compared.
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Modern Trends in Sample Preparation of Biological Material for Analysis of Selected Biologically Active Compounds
Sadílek, Petr ; Solich, Petr (advisor) ; Nobilis, Milan (referee) ; Barták, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Petr Sadílek, M.Sc. Supervisor: Prof. RNDr. Petr Solich, CSc. Co-supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Doctoral Thesis: Modern Trends in Sample Preparation of Biological Material for Analysis of Selected Biologically Active Compounds This doctoral thesis deals with the determination of selected drugs, their metabolites and other biologically active substances in biological materials. This area of instrumental analysis turns out to be very attractive recently. The reason for this is the need to monitor and optimise treatment and the goal is to achieve the most effective therapy with minimum side effects of drugs for every individual; there are also toxicological reasons. The thesis concentrates on the entire analytical phase of laboratory screening, i.e. not just on development and validation of chromatographical methods of analysis, but also (and especially) on preparation of biological samples prior to the actual analysis. This phase is crucial for analytes assessment, since it determines not only correct results of the laboratory examination, but also influences the lifespan of laboratory equipment, especially chromatographic columns. These days mostly traditional...
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Using of leisure time in families with pre-school children in the region of the city Pelhřimov
PETRŮ, Jana
The thesis deals with leisure time in families with pre-school children in the region of the city Pelhřimov. It desribes conception of the leisure time in families with one or more children. The theoretic part talks about family, follows up its meanings and functions in human life. There is a short description of development of the family in modern time and some specifics of the families with younger preschool children too. Then there are desribed relationships in family (depending on the number of children in the family) and an intergenerational learning process. The next section is devoted to leisure time and its role in human life. We will find there a definition of a leisure time, a brief look at the historical evolution of this phenomenon in modern time and some of its specifics in contemporary family life. The work next deals with some socio-psychological characteristics of younger pre-school children with regard for leisure time. There are written some characteristics of the Pelhřimov?s region related to leisure time. The researching part of the work describes how the conception of the leisure time looks in the family of younger pre-school children in the Pelhřimov?s region. It highlights some of the differences in the conception among families with one or more children too. In a selected population sample survey was carried out using semi-structured interviews. They were then processed using a triple-coding grounded theory.
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Development and applications of affinity carrier for isolation of human carbonyl-reducing enzymes
Andrýs, Rudolf ; Wsól, Vladimír (advisor) ; Šebela, Marek (referee) ; Šatínský, Dalibor (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Rudolf Andrýs Supervisor: Prof. Ing. Vladimír Wsól, Ph.D Title of dissertation thesis: DEVELOPMENT AND APPLICATION OF AFFINITY CARRIER FOR ISOLATION OF HUMAN CARBONYL-REDUCING ENZYMES For several millennia the human medicine is based on application of small bioactive molecules that are administered in the form of plant extracts or synthetic compounds. However, their use in modern medicine is not possible without a detailed understanding of their biochemical effects and identification of their molecular targets. Chemical proteomics based on the specific recognition between the bioactive molecule and the target molecule is currently the most widely used techniques for identification of molecular targets of small molecules. Compared to conventional biochemical methods (e.g. 2D electrophoresis), chemical proteomics represents particularly sensitive and very selective technique that enable successful identification of biomolecules from complex biological samples that are naturally presented in very small concentrations. Carbonyl- reducing enzymes, which play an important role in physiology due to their involvement in metabolism of various endogenous (e.g. prostaglandins, steroid...
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Architectural transformation of urban centers. Czech towns 1945 - 1989.
Kurz, Michal ; Klusáková, Luďa (advisor) ; Randák, Jan (referee)
The topic of this thesis is the architectural transformation (i.e. redevelopment and new buildings) of historical centers in Czech towns in the years 1945-1989. The author tries to put this process into context of the period architecture and heritage conservation and to answer some basic questions. How were the conceptions of an optimal form of new buildings intended for historical surroundings being generated and developed? What factors did these conceptions influence? Why were new buildings so often being located just to these valuable town parts and who did in the process of their formation participate? The thesis is divided into five parts which are connected in time and the author emphasizes the topic continuity with the pre-war period, because we can observe the conflict between traditionalism and modernism as well as the advantages and the limits both of these methods as far back as the 1930s. The examples are chosen within the set of 71 Czech district towns except the capital city Prague. Keywords: Modernisation of historical city-centers, urbanism, architecture, heritage, 1945-1989
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