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Comparison of extraction procedures for HPLC determination of liposolubile vitamines in serum
Nápravníková, Lenka ; Solich, Petr (advisor) ; Šatínský, Dalibor (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Lenka Nápravníková Consultant: Prof. RNDr. Petr Solich, CSc. Consultant Specialist: Doc. RNDr. Dagmar Solichová, Ph.D. Diploma Thesis Title: Comparison of extraction procedures for HPLC determination of liposolubile vitamines in serum In this thesis were compared extraction methods, solid phase extraction (SPE) and liquid liquid extraction (LLE) used for separation of human serum fat soluble vitamins: A (retinol), E (α-tocopherol), D2 (ergocalcipherol), D3 (cholecalcipherol), 25- (OH)D3 (calcidiol). Vitamins were then determinated by high performace liquid chromatography (HPLC). As a baseline method used the procedure that was developed and partially validated the thesis: Application of the SPE technology and monolithic columns in HPLC analysis of biologically active substances (Horčičková 2009). This procedure was further optimized in order to increase the extraction of target analytes 25(OH)D3, D2 and D3 and shortening the preanalytical phase. Conditions were tested: sample volume, deproteination of serum (reagents, volume, temperature, time), the conditioning of SPE columns (water washing, the dosage), various SPE columns, elution process (agent, the dosage, use of vacuum), the...
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Development and validation of HPLC method for separation and determination of active substances in pharmaceutical preparation Valetol
Ullrichová, Jana ; Šatínský, Dalibor (advisor) ; Matysová, Ludmila (referee)
Development and validation of HPLC method for separation and determination of active substances in pharmaceutical preparation Valetol Jana Ullrichová Abstract A method for the determination of paracetamol, propyphenazone and caffeine by the high- performance liquid chromatographic method with ultraviolet detection has been developed and validated. The analysis was performed at the room temperature in an isocratic mode on the reversed phase ODS Hypersil 5 µm C-18 column (250x4,6mm). A mobile phase (water : 2-propanol : diethylamine : methanol (50 + 15 + 3 + 32) adjusted to pH 7.5 by means of phosphoric acid) was suitable for the separation and the determination of paracetamol, caffeine and propyphenazone. UV detection was applied at 273 nm. Injected volume was 5 µl, flow rate 0,5 ml.min-1 . The developed method is sensitive and selective and can be applied for the routine studies of pharmaceuticals in the tablet form.
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Surgical sex conversion
Dohnalová, Lenka ; Šamánková, Marie (advisor) ; Kříž, Jan (referee)
The aim of this thesis is to concentrate to inform the comunity with less known problems of nursing the patients with disorders of sexual identification - with transexuality. The persons with this disorder have genetic and somatic equipment belong to one sex, however their sexual identification is orientated to contrary gender. Surgical conversion of sex of transexuals forms oni y one of the aspects by complex approach to this complicated disorder. The decision when the surgery is indicated is up to sexologist and authorized by the committee. The commision decision is based on detailed and prolonged psychological approach to everyone. The observation is pointed at adaptation for new social status a:fter longtime hormonal treatment and follows a:fter operation and o:fficial conversion ofthe gender as well. Powered by TCPDF (www.tcpdf.org)
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Control of house heating
Hnyk, Marek ; Šandera, Josef (referee) ; Šteffan, Pavel (advisor)
The aim of the final thesis is a proposal and implementation of a system which would enable a heating control on the basis of data gathered by monitoring of particular rooms inside a building. In particular, it is focused on water measurement within the heating system, TUV (non-potable hot water) and the temperature within the space of residential rooms. The system headquarters must enable an automated saving of the measured data. Selected temperature figures will be made accessible by a web interface and continuously saved on the web server.
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Consequences of blackouts on agricultural farms of South Bohemia
PARLÁSKOVÁ, Lucie
Electrical energy plays an indispensable role in our contemporary society. However, we mostly realize its position in agriculture in a situation which often already entails irreversible consequences. Therefore, the thesis aims to assess the consequences of power blackout on agricultural farming. In connection with the selected objective, a question arises whether long-term power blackouts also constitute a serious threat to agricultural farms of South Bohemia? The theoretical part briefly describes electrical energy, its production and method of transmission. It also includes basic elements of the power system including its stability and safety. The chapter also acquaints the reader with the critical infrastructure of the Czech Republic and its legislative base. Then, it globally analyzes the term blackout.Given the topic of the thesis, it is necessary to mention the valuable work of the integrated rescue system which in addition to the basic obligations to ensure the protection of human life and property is required to provide missions to rescue animals in emergency situations. Finally, this chapter deals with the application of electrical energy in agriculture, including the characteristics of surveyed farms and a high degree of automation used in farming.The research part is based on the search of the current state of selected agricultural farms. The research was focused on the South Bohemian Region which makes no exception in relation to this issue. Used for the research was a qualitative method of data collection with the information obtained through the techniques of controlled interviews and the secondary analysis of data provided by competent employees of farms in South Bohemia. The results are then outlined in two planes. During recent years, breeding stations have been completely renovated, especially in the section of technological processes. Modern electronic devices which include e.g. automated milking, feeding, suction and washing equipment, heating of service water and heating, should ensure trouble-free operation in such farms. However, what the vast majority of farms is not adequately prepared for is to ensure their smooth operation even during a failure of the electrical system. A number of farms do not have an alternate source of electrical energy. The percentage of self-sufficient farms having on its premises a pumping station that can supply the local diesel-aggregate, is also negligible. Moreover, in case of a diesel-aggregate failure, no alternative source is available. A crucial problem arises in the category of poultry. The animals are situated in large-volume halls where all processes are controlled by fully automated control systems. An alternative source of electrical energy is a crucial element needed to ensure normal operation of the farms. Unless the established criteria are in compliance (ventilation, lighting, etc.), reaction of the animals becomes evident within tens of minutes. Any deviation results in rapid loss of animals. It is assumed that the resulting data will be used both for research purposes in the area of secondary impacts in case of electrical system failures and for objective assessment of the effectiveness in farming security within the current methods. The purpose is to educate the above entities.
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Spatial survey of asset utilization of european companies in 2006
Večerek, Petr ; Marek, Petr (advisor) ; Makovec, Martin (referee)
The content of this thesis is the processing and evaluation of asset utilization ratios of individual components of assets by indicators of total assets turnover, fixed assets turnover, current assets turnover, inventory turnover, accounts receivable turover, creditors turnover and trade deficit. Thesis concerns industry companies from the European Union. Data are from 2006. The values were analyzed separately for all indicators, but with an accent on the greatest complexity. High values of turnover were mostly found in Germany, Ireland and the Nordic countries. Conversely, low values are in all the southern countries and Lithuania. Among the sectors with smallest turnovers are B "Fishing and fish farming" and E "Electricity, gas and water supply". The highest values of turnover were often calculated in sectors DA "Manufacture of food products, beverages and tobacco" and DF "Manufacture of coke, refined petroleum products and nuclear fuel". The values of Czech republic varied just slightly above average, but in the vast majority of indicators.
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ASEAN - establishment, development and perspectives of the Association of Southeast Asian Nations
Chaloupková, Jana ; Veselý, Zdeněk (advisor) ; Krines, Vlastimil (referee) ; Had, Miloslav (referee) ; Gombár, Eduard (referee)
The presented study is an analysis of the regional integration process of the Association of Southeast Asian Nations ASEAN utilizing theories of international relations and integration. The dissertation studies the factors which contributed to its formation, evolution and transformation and the perspectives of its further development. ASEAN was established by virtue of Bangkok Declaration signed on August 8, 1967 among Indonesia, Malaysia, Philippines, Singapore and Thailand. Subsequently Brunei joined the Association in 1984, Vietnam 1995, Laos and Myanmar 1997 and Cambodia 1999. ASEAN's formation falls within the process of creating of regional organizations after World War II, a period of the biggest development of the institutionalization of the international cooperation. Association was based on relatively free and voluntary cooperation and political declarations. With the end of Cold War and bipolarity, with the defeat of communism and the advent of political and economic reforms in the former socialist bloc ASEAN institutionally strengthened and transformed itself, expanded its membership and the decision to form ASEAN Free Trade Area AFTA in 1992 and the Treaty on the Common Effective Preferential Tariffs shifted Association from the stage of cooperation to integration. ASEAN becomes an international organization (based on international legal agreement with the objectives, principles, internal structure etc.) through the adoption of the Charter in 2008. The Charter is the symbol of the transition process from voluntary cooperation based on political declarations to creation of an intergovernmental organization with international legal personality and legal contractual basis for community building, to strengthen the norms and principles. ASEAN has functioned over 40 years based on personal, very frequent and regular contacts between the key elites (who have significantly contributed to its creation and development), as a grouping of common practice and an emerging common identity. It is a form of intergovernmental cooperation, where member states have exclusive position in the decision process; there are no supranational institutions that would have exclusive powers. ASEAN is the initiator of the development of regionalism in Asia, a considerable number of international structures, inter-and trans-regional contacts, forums and programs. Its activities after the Cold War fall into the framework of the new regionalism The thesis tries to point out possible perspectives for its future path, especially in connection to recently born special cooperation ASEAN+3 (China, Republic of Korea, Japan) and plans for building of East Asian Community (ASEAN+3, Australia, New Zealand, India) and its Free Trade Area. Basic characteristics of ASEAN integration process: it takes place in Southeast Asia, it is a process and a state, it consists of economic, political, security, social and cultural part, the emphasis is on economic affairs, its origin is in the postwar period, the main actors are the states and their elites (from the 90's NGO's, think-tanks and civil society become active creating a broad network of socio-cultural relations and interactions), integration expands by the process of ASEAN +3 and EAS, there is a spillover effect within and outside ASEAN, integration process is gradually evolving from a lower to a higher level. Carried out research has its limitations, since it is impossible to generalize the results and formulate a clear assessment of the large international complex, the information is always limited and the social process is miscellaneous. Well-known is the fact that political elites do not act according to the theories, which they often do not know, but based on their own understanding of reality and interests of individual states, eventually groups of countries. No theory is able to explain fully the evolution of ASEAN, failing to capture reality in its full extent, and thus the prospects for the future are some speculations. From the theoretical examination of ASEAN I elect neofuncionalism, since ASEAN represents a process of empowerment, where elites play a crucial role; they share many values and objectives and contribute to the integration process. The rise of transactions in the regional grouping (trade, communication, exchange of ideas), gradually creates a sense of common identity, elites have closer contacts and their values are complementary. Southeast Asia should occupy an important place in the Czech Republic's foreign policy, taking advantage of traditional contacts with the region. Priority should be given to economic ties and active political involvement in the multilateral framework of ASEM, an important part of political dialogue should be question of human rights.
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Analysis of short Argonaute isoforms from mouse oocytes
Jankele, Radek ; Svoboda, Petr (advisor) ; Petr, Jaroslav (referee)
AnalysisofshortArgonauteisoformsfrommouseoocytes Abstract: Argonaute proteins carrying small RNAs form the conserved core of RNA silencing mechanisms, which repress viruses, mobile genetic elements, and genes in a sequence specific manner. The microRNA (miRNA) pathway is a dominant mammalian RNA silencing mechanism in somatic cells, which post-transcriptionally regulates large fraction of genes and thereby adjusts protein levels. miRNA-guided Argonautes inhibit translation and induce deadenylation of complementary mRNAs, ultimately resulting in their decay. In contrast to RNA interference (RNAi), which employs Argonaute slicer activity to directly cleave perfectly complementary RNAs, an effective miRNA-mediated mRNA repression requires multiple Argonaute-associated protein factors and enzymes. The miRNA pathway has been implicated in many complex biological processes ranging from organogenesis, stress-response to haematopoiesis or cancer. Surprisingly, canonical miRNAs are not essential for oocytes and early embryonic development in mice. Even the most abundant miRNAs present in mouse oocytes are unable to effectively repress target genes. However, RNAi, which shares key enzymes with the miRNA pathway, is highly active in oocytes and early embryos. The cause of miRNA inactivity in mouse oocytes remains...
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Optimization and Statistics
Fink, Jiří ; Kratochvíl, Jan (referee) ; Loebl, Martin (advisor)
One of the basic streams of modern statistics physics is an effort to understand the frustration and chaos. The basic model to study these phenomena is the finite dimensional Edwards-Anderson Ising model. In discrete optimisation this corresponds to the minimal T-joins in a finite lattice with random weights of edges. This thesis studies a random join which is a random path between two given vertices. The original definition of the random join is very complex, and we have managed to find an equivalent one which is more natural. We use our definition to exactly compute the random join on circles. We also propose an algorithm which finds the shortest path in a large lattice with given weights of edges. This algorithm can be used for an experimental study of the random join.
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The Assessment of Pain by a Nurse and the Control of Post-operative Pain
Hanousková, Jana ; Chloubová, Helena (advisor) ; Di Cara, Veronika (referee)
This diploma work is focused on post-surgical pain. It lays out the role of a nurse in pain assessment, factors influencing post-surgical pain and ways of its controlling. It also publishes the results of the research organized in the surgical fields of the Central Military Hospital Prague in January and February 2006. The work is structured into two large parts, theoretical and empirical one. The diploma work underlines the importance of the team cooperation of all those who participate in pain management and in individualized complex nursing care for a patient in the past-surgical time period.
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