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Study of exotic hypernuclei
Posolda, Petr ; Mareš, Jiří (advisor) ; Kvasil, Jan (referee)
The thesis focuses on the study of properties of exotic hypernuclei, particularly of beryllium, carbon, oxygen and neon isotopes with and hyperons. Calculations were performed in the framework of the relativistic mean field theory (RMF) where a (hyper)nucleus is treated as a system of Dirac spinors (nucleons, hyperons) interacting via (mean) meson fields. The exotic hypernuclei were considered as axial symmetric. Up to now, hypernuclear calculations have been performed under assumption of spherical symmetry. This work thus extends hypernuclear calculations to the region of exotic, generally deformed systems. For the above nuclei, the numerical calculations of the binding energies and root mean square radii were performed. Moreover, we studied influence of the tensor interaction between meson and hyperon on the spin-orbit splitting. The results confirmed that the presence of the hyperon increases values of the binding energy of a system and on the contrary, it decreases its root mean square radius. We studied the possibility of the existence of the hyperon bound states in a nucleus. For the above isotopes, no bound states were found for the + hyperons. On the other hand, weekly bound states of the - hyperon are predicted for several isotopes.
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The role of sumoylation in cellular senescence
Kopová, Ivana ; Švadlenka, Jan (referee) ; Hodný, Zdeněk (advisor)
Organisms with renewable tissues require mechanisms to prevent the development of cancer. One such mechanism is cellular senescence, which irreversibly arrests the growth of cells at risk for neoplastic transformation. In this study, we show that 100 μM 5-bromo-2-deoxyuridine, 0.5 μM camptothecin, 0.5 μM aphidicolin and 2.5 mM thymidine cause chemically-induced premature senescence in different human cancer cell lines and they induce an increasing conjugation of SUMO-2/3 isoforms. Chemically- induced premature senescence also induces formation of SUMO-1 and SUMO-2/3 foci, which are colocalized with PML nuclear bodies. In addition, we describe that aphidicolin induces premature senescence in stable HeLa cell line expressing His6-tagged SUMO-1. HeLa-His6-SUMO-1 cell line has decreased number of PML bodies, which do not colocalize with SUMO-2/3 foci. Moreover, the number of PML bodies in HeLa cell line with ectopic expression of His6-SUMO-1 is not increasing during aphidicolin-induced senescence. This demonstrates that increasing number of PML nuclear bodies is not essential for aphidicolin-induced senescence. Absence of SUMO-2/3 foci and increased number of PML nuclear bodies support the theory that SUMO-1 acts as a SUMO-2/3 polymeric chain terminator. On the other hand, in stable HeLa cell lines expressing...
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Specificities of nursing newborn children with partum cranial injuries
KOBLIHOVÁ, Jitka
Birth injuries of the new-born infants might be caused either by mechanical or anoxic traumas that occur during the birth. The injuries might be a result of insufficient attention or inadequate efforts of the obstetrician but might as well occur completely independently of the obstetrician´s activities. The character and extent of birth injuries are very wide and vary from the insignificant ones that are cured completely without any consequences to those that leave long-term consequences and that may even end in the death of the new-born infant. Cranial injuries of the new-born infants include: caput succedaneum (swollen scalp), cephalohaematoma, petechiae, suffusion, eye injuries, scalpel-incised wounds, parietal bones fractures, spinal cord injuries and intracranial haemorrhage. This Baccalaureate thesis surveys the knowledge of the nursing staff as far as birth cranial injuries are concerned and concentrates also at specific aspects of nursing new-born infants who suffer from caput succedaneum (swollen scalp) and cephalohaematoma. The theoretical part comprises the most important pieces of medical knowledge in nursing new-born infants with birth cranial injuries. The first goal of this thesis was to map the level of knowledge of the birth cranial injuries among the nursing staff. In compliance with this goal a hypothesis was stipulated: ?Members of the nursing staff are well-informed about birth cranial injuries.? This hypothesis was verified and confirmed. It was found out that members of the nursing staff have good knowledge about birth cranial injuries, their predispositions and prevention. A quantitative research was carried out using the questioning method (the questionnaire technique was used). The research was carried out at the Neonatal Departments of the hospitals Nemocnice České Budějovice, a.s, Nemocnice Písek, a. s. and Nemocnice Strakonice, a.s. The second goal of this thesis was to map the specific aspects of nursing new-born infants who suffer from caput succedaneum (swollen scalp) and cephalohaematoma. In compliance with this goal a research question was stipulated: ?What are the specific aspects of nursing the new-born infants who suffer from caput succedaneum and cephalohaematoma?? It was found out that nursing new-born infants with caput succedaneum and cephalohaematoma does not comprise many specific aspects. The most important thing is to nurse such infants carefully and with caution, to position their heads on the side without caput succedaneum or cephalohaematoma, to check regularly the bilirubin level, to observe carefully the cephalohaematoma or caput succedaneum (its colour, size, localization) and to watch the behaviour of the new-born, mainly whether it shows any signs of pain. The research was carried out in a qualitative way using the question method ? the depth interview technique. The research file consisted of two nurses and two midwives from the Neonatal Department of the České Budějovice Hospital, a.s. The research has revealed that the nursing staff at neonatal departments is well informed about birth cranial injuries and that the nursing methods applied at new-born infants who suffer from caput succedaneum and cephalohaematoma do not differ much from the methods used in case of physiological infants. The output of the thesis is an educative material concerning birth cranial injuries that is intended for the nursing staff at neonatal departments.
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Creation and testing of educational program: Health education and prevention of non-infectious disorders (asthma, chronic obstructive pulmonary disease)
CHUDÁRKOVÁ, Kateřina
The bachelor thesis deals with the design and applicability of an educational program intended for students of the master course Education with a focus on health education. The educational program is aimed at health education and the prevention of non-infectious diseases asthma and chronic obstructive pulmonary disease. In the introductory theoretical section I analyse concepts like health, prevention, lifestyle and disease. The central theme focuses on the chronic obstructive pulmonary disease, asthma and smoking, which has a decisive role in the development of both diseases. In the practical section I verify the applicability of the educational program by comparing the input and output tests, which give evidence about the level of knowledge on the topic in question of randomly chosen students of the course Education with a focus on health education. The results of the practical investigation confirm the feasibility of the program in practical use and its considerable impact on deepening students' knowledge in the studied topic.
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The electrostimulation the spermiation in the crayfish
KUBEC, Jan
The crayfish are important and indispensable animals in aquaculture. In the aquatic environment, they have a role as predators, food for other fish, detrivors and they are an important element in the circulation of the energy and the nutrient in the aquatic ecosystem. Today, number of the european crayfish species populations is decreasing. It is caused by unfavourable regulation of streams, water pollution and the occurence of crayfish plague. The main purpose of present study was examining the impact of electrostimulation on the males of the crayfish and obtaining sex products (the sperm). The principle of this research was determining whether the male of crayfish are able to extrude spermatophore with sperm by an electric stimulus. The transformer was used as resource alternating current in experiments. All experiments conducted in a laboratory of experimental device for fish farming in Vodňany. Experiments were conducted in 2011 on a male of the spiny-cheek crayfish (Orconectes limosus), the signal crayfish (Pacifastacus leniusculus) and the red swamp crayfish (Procambarus clarkii). The results of this work and success of the experiments suggest, that electrostimulation could be used for obtaining sex products (the sperm) from crayfish males. Results from a laboratory of electron microscopy confirm the harmlessness of electrostimulation on the shape and structure of sperm. The sperm can be used to artifical insemination of the crayfish eggs in future. However, sperm capacitation is problematic.
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Soft drinks and their effects on health
Ramstad, Gunnar Alexander Wilson ; Anděl, Michal (advisor)
The consumption of soft drinks has increased manifold over the last decades. In parallel to this increase it has been noticed an increase in health problems, especially obesity. Dentists continue to urge their patients not to drink soft drinks, and there has also been suggested a link between soft drink consumption and osteoporosis, hypokalemia and diabetes mellitus 2. Other additives, like caffeine, have been associated with headaches, restlessness and trouble with sleeping. Although more research is needed to confirm these relationships, many scientists suggest that we should start taking action to prevent children from developing the habits of large soft drink consumption we witness today. This could be accomplished by cooperation between parents, health practitioners, school system and government.
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Oxidative stress induced by iron and the influence of flavonoids and bisphosphonates
Kolek, Metoděj ; Eybl, Vladislav (advisor) ; Habermann, Vlastimil (referee) ; Hrdina, Radomír (referee)
Iron is an essential element for living organisms. However, as it is a transition metal, it can participate in Fenton reaction resulting in generation of free radicals and oxidative damage to tissues. Antioxidants may prevent possible iron toxicity by chelating free iron or scavenging free radicals. Falvonoids are naturally occurring substances that are capable of formation of complexes with metals, including iron. T h e y have been show to possess antioxidant activity, which depends on molecular complexity of numerous types of flavonoids, e.g. quercetin and silibinin. Bisphosphonates are synthetic drugs used to treat various metabolic diseases of bones. Their principál effect is an inhibition of osteclast activity leading to a decreased bone resorption. Bisphosphonates have been however shown to exert some antioxidant activity in in vitro experiments, too. The aim of this PhD thesis was to investigate the role of iron in toxicity of other metals (cadmium) and the effect of flavonoids (quercetin and silibinin) and bisphosphonates (clodronate, etidronate and risedronate) on iron-induced oxidative damage in vivo. Experiments were performed in male mice (CD-1, Charles River, 25-35 body weight). Iron was administered intraperitoneally or in the diet. Cadmium was administered subcutaneously. Flavonoids and...
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Diseases caused by ionizing radiation and their prevention
Hřib, Zdeněk ; Hrnčíř, Evžen (advisor)
3 SUMMARY The text summarizes some of the newest knowledge about effects of the ionizing radiation on various levels of the biological systems and recent recommendations for radiological protection. First chapter of this diploma work gives a brief definition of ionizing radiation, possible sources, summary of its interactions with matter and description of the dosimetric quantities. Second chapter focuses on biological effects of the ionizing radiation. With development of micro-beam technology, the newest research has finally confirmed the assumption of DNA as the main target cell structure for ionizing radiation damage. Apart from well known stochastic effects (cancerogenic and those, causing hereditary and chronic diseases) caused by non-lethal cell damage and organ and tissue reaction (formerly known as non-stochastic or deterministic effect, eg. acute radiation syndrome, acute local damage, chronic radiodermatitis and cataract) caused by lethal cell damage also issues like low dose threshold, in utero exposure risk and newest recommendations for weighting factors are discussed here. Last chapter is dedicated to targets and principles of radiological protection for patients, workers and other population.
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The issue of prevention and early detection for patients with vertebrogenic syndrome in the cervical spine
HORKÁ, Michaela
This bachelor thesis deals with preventive measures and early detection of the vertebrogenic algic syndrome in the cervical spine. Vertebrogenic algic syndrome is a pain in different parts of the spine, which is one of the most common reasons for doctor visits. An alarming fact is that up to 80% of adults face these difficulties up and age of patients is constantly decreasing. Most common cause is poor posture, cervical spine injuries, mental health, degenerative changes, tumors and more. Part of this work is devoted to the prevention of vertebral problems. In these modern times, when the use of computers, laptops, tablets, became the ordinary matter, it is necessary to raise awareness of the basic ergonomic principles when using these devices, modifying work and home environment. The theoretical part is supposed to inform about the causes, mechanisms and physiotherapy practice in the treatment of vertebrogenic algic syndrome. It includes basic knowledge of anatomy, biomechanics of the cervical spine and preventive measures concerning the ergonomics of the working environment, sleeping position and appropriate sports activities. For the practical part of the thesis has been used qualitative research method. Qualitative research was done in the form of case studies of three persons. Each was subjected to an input and output kinesiology examination and history-taking in the form of a structured interview. The probands were interviewed regarding home and work ergonomics. The therapy lasted five weeks at the Health centre Medipont in České Budějovice. The task of the practical part was to use physiotherapy techniques to alleviate pain in a particular segments of the cervical spine and report about basic preventive measures. The result is the production of an educational booklet that contains exercises to stretch and release the cervical spine, basic ergonomic positions accompanied by photographs. This brochure will be available at the front desk at the Health centre Medipont in České Budějovice, in waiting rooms of general practitioners in Jemnice as a practical and synoptical helper in the fight against the problems in the cervical spine. This bachelor thesis could increase an awareness among general public about preventive measures and how to prevent pain in the cervical spine. Furthermore, it can be used by clinical practice physiotherapists in the treatment of vertebrogenic algic syndrome.
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The usage of algae in biogas transformation
Paroulkova, P. ; Sukačová, Kateřina ; Murgasová, K. ; Vítěz, T. ; Chovanec, J.
Using of algae in a biogas transformation is still in the beginning. However, the microalgae have large potential from the perspective of growing demands on biogas quality and trend of using,natural resources. First of all, it is their ability to fix carbon dioxide (CO2) using photosynthesis and presumed ability of some algae to metabolize hydrogen sulphide (H2S). Biogas contains not only required methane but also components causing its worse quality such as mentioned CO2 and H2S. Therefore, the algae are potential biological systems for biogas-conditioning. The microalga Chlorella pyrenoidosa Chick (IPPAS C2) was used for fixation of CO2 and H2S in our experiment. The microalgae were cultivated in a medium BG 11. The algal suspension was aerated with the biogas during two weeks. Different values of CO2 concentration measured in the input and output confirmed decrease of CO2 caused by intensive growth of algal culture. Decline of H2S was not confirmed.
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