|
|
The view of statut human embryo from ethical point of view
BŘEZINOVÁ, Nicola
The work deals with looking at the status of the human embryo. The structure of the work is divided into several chapters that deal with a particular topic. The first chapter deals with the historical view of the adoption of a human embryo, in the second chapter is devoted to philosophical adopting the human embryo and view individual philosophers on this issue. The third chapter is devoted to the ethical acceptance of the issue. Here is discussed an ethical perspective on human embryo status and access two main streams which occur in this area. The next part is devoted to the present, both in terms of the ethical-philosophical, and in terms of health. In the medical section includes prenatal communication between mother and child, and research on embryonic stem cells, which is associated with a number of ethical issues.
|
|
|
Legal and ethical status of the human embryo through the eyes of the young generation
WÁGNEROVÁ, Lucie
There has been the question of the approach to the unborn human foetus which has been dealt with for many years. The moral, ethic or legal questions appear during the discussions. In this context we could find many different opinions related to the upbringing, religion or morality. It is hard to find any general agreement. Nowadays there are new questions connected to the intervention of reproduction, the research of embryo cells or the issues of abortion, which is perceived as the women's right. There are about 44 billions of abortions these days. According to these problems there is the need of the social discussion about the understanding of embryo and when we can consider it as a human being. The theoretical part of my bachelor thesis deals with the legal point of view to the role of human embryo and its securing in our legal system. The Charter of Fundamental Rights and Freedoms, which is a part of the Constitution of the Czech Republic, determines that everybody has the right for living as well as the human life should be protected before the delivery. There is not and there also cannot be determined clearly when the human life begins. The answer for this question is connected to the moral and ethic principles. My thesis deals with the different points of view to this question, if the fertilized ovum is or is not the human. There are also many various arguments about when we can consider the genesis of human life. The answer for these questions is not clear and we cannot find out the definite concordance. The other part of my thesis deals with ethic dilemmas connected to the abortion and the intervention to the reproduction as well as the problems of cloning. The practical part of my bachelor's thesis deals with the legal and ethic status of embryo mainly to the questions of the genesis of human life and the problems of the abortion. This thesis do not provide the clear answer to the question since when we can date the genesis of the life but it represents the opinions of the young generation (18 - 30 years old). The main aim of the research was to find out the point of view of the young people in the Czech Republic. There were questions about the reaction to the unborn child, the genesis of human life, who decides about the life of the unborn child and the abortion. I compare the questions according to the sex, ages, religion and parenthood. The practical part was made by the quantitative research questionnaires. It consists of 250 respondents who were asked by the on-line or printed questionnaire. It was chosen the young generation between 18 - 30 years old. The questionnaire contains seven fact-finding questions and four distinctive ones. It was worked with the data which were acquired from the questionnaire. The first hypothesis, 50% of respondents believe that the human life begins with the conception, was not confirmed. There were only 30% of respondents who believe that question. The second hypothesis, the approval of abortion, was confirmed because there were 90% respondents who agreed. The third hypothesis could not be confirmed. In the discussion the results were compared with the opinions of the authors from the theoretical part of my thesis. The end of my thesis deals with the general results. We cannot find the right answer to the question of the beginning of human life. Every person has a subjective point of view. We should realize every possible risks and think about who has the right to intervene to the substance of human life and how far can technology goes. This bachelor's thesis do not have the clear opinion but it provides the facts and the suggestion for thinking for creating its own attitude to these questions.
|
|
|
Regulation of development of mouse parthenogenetic embryos
Jettmarová, Dominika ; Fulka, Josef (advisor) ; Kaňka, Jiří (referee)
The development of mouse (Mus musculus) haploid parthenogenetic embryos does not reach the same level as normal embryos. The aim of this diploma thesis was to find out whether haploid parthenogenetic embryos of mice differ in the nucleocytoplasmic ratio. The volume of the nucleus increases with ploidity. The nucleocytoplasmic ratios of haploid embryos do not significantly change between the two-cell and four-cell stage (p = 0.052), there is a significant difference (p < 0.001) for diploid and tetraploid embryos. Non-standard nucleocytoplasmic ratio could be related to the problematic development. Understanding the regulation of preimplantational development of parthenogenetic embryos will increase the efficiency of haploid embryonic stem cell derivation.
|
|
|
Effect of incubation temperature on growth of the working and conducting myocardium in the embryonic chick
Skuhrová, Kristýna ; Sedmera, David (advisor) ; Gregorovičová, Martina (referee)
It was shown almost 50 years ago that hypothermic incubation of chicken embryos results in a reduction in the size of embryos and an increase in the heart weight, presumably by hypertrophy (increase in cell volume). The chicken embryos were incubated in normothermia (37.5 ř C) and hypothermia (33.5 ř C) from the eleventh embryonic day. On the 17th day, the embryos were weighed and then their hearts were weighed. In agreement with the previous results, hypothermic embryos were 29% smaller and their hearts 18% heavier. The heart-to-body weight ratio was 67% higher in the hypothermic group. The measured cell size was very similar in the target areas and it was also between the two groups. The left ventricle width was twofold that the right one and the difference was not significantly higher in the hypothermia model. Purkinje fibers, the terminal part of the conduction system, were smaller than the working cardiomyocytes. Purkinje fibers were slightly enlarged after hypothermic incubation. The proliferation rate was measured by immunohistochemical labeling of anti-phospho histone H3. The experimental group showed much higher proliferation rate; it reached statistical significance in the right ventricle. Thus, hypothermic incubation resulted in increased growth of embryonic heart based on hyperplasia...
|
|
|
Legal aspects of stem cells research
Česáková, Barbora ; Šustek, Petr (advisor) ; Salač, Josef (referee)
This thesis deals with the issue of stem cell research and its legal aspects. The issue raises many ethical questions, especially due to its closed connection to the topics of embryonic research and right to life. In the first introductory chapter the thesis explains the topic of stem cells from biological point of view and defines and classifies stem cells by their origin and differential potential. In the second chapter the thesis briefly summarizes the history of stem cell research and deals with the ethical questions raised by closed connection of the topics of embryonic stem cell research and in vitro fertilization to the right to life. The second chapter also notes some recent possibilities and medical methods connected with stem cells, such as gene therapy and diagnostics and new eugenics. In the following chapters the thesis concentrates on the legal aspects of the topic on both national and international level. Specifically, the third chapter puts the issue of stem cells research to the contexts of the two most important conventions - the Convention for the Protection of Human Rights and Fundamental Freedoms and the Convention on Human Rights and Biomedicine. The fourth chapter concentrates on legislation of Czech Republic regulating the legal status of embryo and its protection in the...
|
|
|
Mode of reproduction and reproductive assurance of diploid and polyploid hawkweeds (Hieracium s. str.)
Zdvořák, Pavel ; Mráz, Patrik (advisor) ; Krahulcová, Anna (referee)
The mode of reproduction can greatly influence the demography and the evolutionary success of the taxon. In the case of autonomous asexual formation seeds are apomictic taxa fully independent of pollinators and compatible partners. For sexual taxa with strict autoincompatibility it is the opposite, i.e. sexual taxa need pollinators and compatible partners for birth of offspring. Therefore, in marginal population and for more extreme situation with lower pollinating activity will have apomictic taxa a higher level of reproductive assurance than sexual taxa vascular plants. This hypothesis was tested in natural populations of apomictic and sexual taxa. In the diploma thesis we therefore investigate the method mode of reproduction and reproductive assurance of 52 taxa of the genus Hieracium s. str. (family Asteraceae) in Europe. Of these, 12 were diploid sexually diploid taxa and 42 polyploid apomictic reproductive taxa. From these taxa we harvested seeds from fully developed capitulum and we determined the potential (total number of seeds in the capitulum) and the realized (the percentage of well-developed seeds at the capitulum). The ploidy of the offspring (the embryos and the seedling) and method origins of seeds we examined using flow cytometry. The results show that the plants of diploid species...
|
|
|
Development and function of beta-cells
Hamplová, Adéla ; Pavlínková, Gabriela (advisor) ; Tlapáková, Tereza (referee)
Insulin producing β-cells are located in the endocrine pancreas. They are a part of pancreatic islets of Langerhans along with α-, β-, δ-, ε- a PP-cells producing glucagon, somatostatin, ghrelin and pancreatic polypeptide. Insulin regulates glucose uptake into cells and thus contributes to the regulation of energy metabolism. The development of β-cells as well as the development of the pancreas is a complex process. Developmental processes of proliferation, differentiation and total pancreatic organogenesis are best described in the mouse model. The developmental processes and pancreatic functions are regulated by a network of transcription factors. Pancreatic duodenal homeobox gene 1 is a transcription factor that is expressed in the precursors of endocrine, exocrine and ductal cells. Neurogenin 3 is expressed in precursors of the islets of Langerhans cells. Islet 1 regulates the formation of the islets of Langerhans as well as the pair domains of transcription factors 4 and 6, whose expression is later limited only to β-cells. Transcription factors Islet 1 and Neurod 1 regulate insulin production in β-cells. Mutations in transcription factors lead to the abnormal development and altered function of pancreatic cells, including β-cells. Diabetes mellitus is a disease resulting from defects in...
|
|
|
Role of Nkx2.5 in development and electrophysiology of the mouse heart
Hámor, Peter ; Sedmera, David (advisor) ; Neckář, Jan (referee)
Role of Nkx2.5 in development and electrophysiology of the mouse heart Prague 2016 Peter Hámor, B.S. ABSTRACT The objective of this thesis is to investigate the role of Nkx2.5 gene dosage on electrophysiology of the mouse heart in prenatal stage of its development. The main goal of this work is to search for differences in conduction of electric impulses through the embryonic mouse hearts of different genotype. Special method of capturing the conduction of electric impulse through myocardium, called optical mapping, was used to visualize the electrical activity. Thanks to this method I was able to construct images and videos capturing the spread of the impulse with identification of the beginning of the activation and its direction in the heart. These outputs, or optical maps, help to define anomalies and defects in mutants compared with a normal functioning heart. The thesis focuses on the expression of the transcription factor Nkx2.5 and regulatory components related with the correct formation and physiology of the heart until 9.5 days post coitum. Embryos at this developmental stage were optically mapped and analysed according to their genotype. While the wild type and heterozygote mouse embryos exhibited high degree of similarity, the homozygous mutants were dramatically different. Considering this work...
|
|
|
Legal aspects of the research into stem cells
Tichá, Jolana ; Šustek, Petr (advisor) ; Salač, Josef (referee)
- anglicky This thesis deals with legal aspects of stem cell research, which in the case of the Czech Republic remains quite disregarded by the general public. The text examines and analyzes the legislation regulating the aforementioned field of research at the international, European and Czech national level in a comprehensive manner. Particular emphasis is placed on legal aspects of embryonic stem cell research, which is also closely related to the legal status of embryo and its protection. The thesis also notes what types of regulatory approaches are adopted in various countries with respect to stem cell research and it subsequently demonstrates these conclusions by examples of Slovak Republic and the United Kingdom. Explanation is set in a broader framework through the introductory chapters that deal with biological and ethical aspects of stem cell research.
|
|
|
Legal aspects of the research of stem cells
Šolc, Martin ; Šustek, Petr (advisor) ; Salač, Josef (referee)
The thesis deals with an issue of legal aspects of stem cell research with a special emphasis on the use of embryonic stem cells which is connected with the most of ethical and legislative controversies. At first, there are presented biological and ethical aspects of the topic necessary for its understanding. The thesis further analyses related legislation relevant for the research conducted in the Czech Republic. With respect to international law, the thesis focuses mainly on the Council of Europe system of protection of human rights. Then, the thesis presents regulation in primary and secondary European Union law. In the two final chapters, there is presented Czech regulation in the constitutional order (in the Charter of Fundamental Rights and Freedoms) and laws, which is then viewed in European context as moderately permissive and relatively well prepared. Its greatest weakness is the lack of discussion about the topic in the Czech public.
|
guest ::
