|
Expression of SWC7 molecules on the pig B lymphocytes.
Fikrová, Petra ; Konečná, Klára (referee) ; Jílek, Petr (advisor)
The aim of this graduation thesis is to clarify the expression profile of the SWC7 molecule on B cells in swine and eventually to resolve its function during maturation. At present it is possible to define the maturation pathways of porcine B lymphocytes in the periphery on the base of the expression CD2 and CD21 cell surface molecules. The flow cytometry was chosen as a central experimental method for detection of B lymphocytes carrying SWC7 molecule on their surface. The ability of individual B cell subpopulation to produce antibodies was also measured by ELISA. Principal phenotype characterization showed that the SWC7 molecule is found only on the surface of IgM+ and CD21+ B cells. These may be CD2 positive or negative. Based on this finding, the individual subpopulations of IgM+ CD21+ B cells differing in expression of SWC7 and CD2 molecules were purified by flow cytometry. Subpopulations were thereafter cultivated and subsequently analyzed for alterations in their phenotype. Media from cultivated cells were also analyzed for presence of porcine IgM, IgG and IgA antibodies by ELISA. Results indicate that the expression of SWC7 molecule on the surface of B cells may be considered as a maturation marker because loss of SWC7 molecules from the cell surface is irreversible and because only SWC7- B cells are...
|
|
The ornamental park tree species as a resource of the Rhabdoviridae family viruses
PECKOVÁ, Lucie
Rhabdoviridae family viruses attacking the plant hosts were only described at the angiosperms. In this work, a gymnosperm rhabdoviridae infection was described for the first time ever ? specifically at Ginkgo biloba. Even though there were not observed any kinds of obvious infection symptoms on any of randomly chosen plant samples, through the molecular methods and detection primers the rhabdoviridae infection was proved at six of the plant samples. The acquired nucleotide and amino acid sequences, which were compared with the GenBank sequences, confirm the Rhabdoviridae family viruses occurrence. These given sequences demonstrated a certain analogy with a Strawberry crinkle virus assigned to the genus of Cytorhabdoviruses. The analyses proved a different reciprocal homology among the nucleotide sequences of the individual isolates, and in all likelihood an occurrence of two up to now unknown viruses in the Ginkgo biloba samples was proved for the first time. A definite categorization will be dependent on an acquisition and comparison of other sequences from the isolates genome and also on certain biological characteristics observation.
|
|
Analysis of the LMNA gene and the SH3TC2 gene among Czech patients with hereditary neuropathy Charcot-Marie-Tooth type 1 and 2
Laššuthová, Petra ; Seeman, Pavel (advisor) ; Martásek, Pavel (referee) ; Fajkusová, Lenka (referee)
My PhD thesis can be devided into two parts: 1. Hereditary motor-sensory neuropathies (HMSN) 2. Selected muscle disorders The main emphasis was on the first part - hereditary motor and sensory neuropathies. Research was focused on autosomal recessive forms - demyelinating type CMT4C and axonal type CMT2B1. Most of the results obtained are related to these disorders. Data, which were obtained, are unique and were published in international journals with impact factor. Results obtained from CMT4C study are accepted for publication in Clinical Genetics. Results obtained in LMNA study (CMT2B1) were published in Journal of Human Genetics. The author performed and validated these new methods and original results, which are due to be used in genetic molecular testing of patients with hereditary neuropathies and muscle disorders: 1. Sequencing of all coding exons of the SH3TC2 gene. First mutations in the SH3TC2 gene in Czech HMSN I patients were found. 2. The prevalent mutation among Czech CMT4C patients was proven to be p.Arg954Stop. 3. Real-time PCR assay targeted at detection of the prevalent mutation p.Arg954Stop in the SH3TC2 gene was validated and is now used in our lab on a daily basis as a quick and efficient screening. 4. Molecular genetic testing of the SH3TC2 gene was introduced into the routine...
|
| |
|
Phytochemical investigation of above-ground parts of Magnolia Vine
Dadáková, Petra ; Opletal, Lubomír (advisor) ; Karlíčková, Jana (referee)
(ABSTRACT) Petra Dadakova: Phytochemical Research of the Aerial Part of Schizandra Chinensis (TURZ.)BAILL. The work was aimed at isolating of at least one constituent from the polar n-butanolic extract of the leaves and cauloms of Schizandra chinensis (TURZ.)BAILL., Schizandraceae, and at physico-chemical characterization of the isolated substance. The separation was performed by means of column and preparatory thin layer chromatography. Two pure compounds were obtained - stigmasterol glucoside and shikimic acid - and their structures were determined by NMR spectroscopy. Moreover, 18 crystalline fractions containing mainly one compoud or a mixture of several compounds were separated. According to TLC combined with the usage of specific detection reagents, these fractions contain predominantly flavonoid substances, e.g. quercetin, kaempferol, rutin and/or their glycosides, other phenolic substances such as cinnamic acid, and saccharides. The constituents typical for fruits (seeds), i.e. essential oil and aproximatelly 60 lignans of dibenzo[a,c]cyclooctadiene type, are contained in individual morphological parts, i.e. cauloms, leaves and seeds in the approximate ratio 2.4 : 1 : 11.4, respectively. Hence, cauloms and leaves cannot serve as primary source of lignans with strong antioxidative,...
|
|
Krystalinita a slinovatelnost wolframu
Brožek, Vlastimil ; Maixner, J. ; Domlátil, J. ; Janča, J. ; Eliáš, M.
Sintering activity of tungsten powders of various origin was tested by modified technique according Agte. The size of crystallites calculated according to Scherrer formula corresponds with density values detected by Mercury porosimetry. Tungsten poder obtained by reduction of tungstates in hydrogen plasma can be recovered to 93 % of theoretical density at the temperature 1350°C. Next increase of the density needs simultaneous application of high pressure. By the BELT technique 95 % density was reached, by plasma spheroidization up to 98 %.
|
|
Rapid detection microogranism in pharmaceutical materials.
Novosád, Aleš ; Jílek, Petr (advisor) ; Voxová, Barbora (referee)
1 Summary Many microbiology laboratory, no matter what already in pharmaceutical firms or to hygienic stations ground and firms conversant food production used to analysis big quantity microbiology samples conventional techniques based on cultivation appropriate culture. These techniques take hours to days to yield a result, are tedious and are not suitable for non- culturable microorganisms. Further, culture-based techniques do not provide real-time information on the physiological status of the organism in situ which is important in the industrial manufacture of many microbial products (1) and often at high of the number of micro - organisms, when isn't possibility number micro - organisms determine, all time cultivation about next dilution sample elongate and about time growth this micro - organism. Flow cytometry makes it possible to obtaining real microbial determination single micro - organisms, without dependencies on type microbial culture. Nevertheless, flow cytometry has not been extensively used as a tool for routine microbial analysis. Reason is mainly high cost and complexity of instrumentation, the need for trained flow cytometrists and the lack of assay kits with appropriate biological reagents for specific applications. Much modern instruments are now relatively simply serviceable, thanks...
|
|
Aesthetic and Body Movement Education in the Waldorf Pedagogy.
Šimková, Marie ; Mazáčová, Nataša (advisor) ; Váňová, Miroslava (referee)
Over the last few decades there has been a detectable increase in public awareness of the complexity of human being. While in the former years cognitive faculty (measured by IQ tests) was the main matter of psychological interest, nowadays experts are focusing more on the other components of mental activity: emotional, social and creative intelligence. It is beyond dispute that school should foster the development of all these qualities; however, a question rises over the educational procedures and methods which should be employed to reach this high idea. One of the possible ways is represented by waldorf pedagogy whose main aim is to nourish and develop children's minds, hearts and souls and guide them on their way towards becoming valuable members of human society.
|
|
Depth Map Estimation from Stereo Images
Berek, Daniel ; Hradiš, Michal (referee) ; Španěl, Michal (advisor)
This thesis is dedicated to the problem of stereo vision and 3D reconstruction from stereo image. Spatial perception is realized by using depth map, which is created by using disparity map. Main goal of this work is to create an application for estimating object distance from car using methods for depth map estimation. The work also includes description of main steps to achieve goal, like image capturing, stereo camera calibration, image rectification and disparity estimation. The result of this work is an experimental solution for monitoring system used in cars, which focuses on pedestrian detection in front of the car, and calculates its distance by using depth map. Part of this work is also creating test set, which is used for evaluating the accuracy of this system.
|
|
The Indication of Earth Faults in Overhead Lines
Pospíšil, Zdeněk ; Topolánek, David (referee) ; Toman, Petr (advisor)
This master´s thesis deals with the indication and localization of earth faults in overhead lines. Earth fault is the most frequently occurring type of fault in medium voltage overhead lines – it covers approx. 95% of all faults and is very difficult to indicate and localize them correctly and in time with currently available methods on the market. Therefore is very important to study earth fault and its indication, localization. The thesis consists of a theoretical and a practical part. The theoretical part deals with faults in overhead networks with different type of neutral grounding, mainly with one phase to the ground fault in the compensated, ungrounded, solidly grounded and via resistance grounded networks. Most of the theoretical part is dedicated to one phase to the ground fault in the compensated and ungrounded networks, where this type fault is called the earth fault. In the compensated and ungrounded networks is described in details behavior – voltage and current relations during both steady state and transient state earth fault. The theoretical part is further dedicated to detection methods of earth faults and their preconditions for use. There is described also in details the complete procedure of earth fault detection, which includes indication, unhealthy feeder determination and exact position or line section localization. End of the theoretical part is then focused on determination of accuracy requirements for measurement of basic quantities and computation of other parameters. The practical part deals with a work at medium distribution network model, which includes familiarization with the model, detailed verification of its functionality and behavior during the earth fault, obtaining faults records and algorithmization of methods: method of qu – diagram and method of first half - period, which are able to detect unhealthy feeder. This part of the thesis was put together based on a demand of company Mega, corp., which wanted to verify function of both above mentioned and by them not yet tested methods.
|
guest ::
