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Walking dysfunction in multiple sclerosis
Doležalová, Vendula ; Řasová, Kamila (advisor) ; Zimová, Denisa (referee)
In this study, we tried to find out what information we will get through the walk tests, if the walk tests provide different information for people with varying severity of the disease. From July to August 2009, patients with multiple sclerosis passed a cerebrospinal investigation carried out by two trained students from the 3rd Medical Faculty of Charles University. The whole study conducted in collaboration with MS Center Neurological Clinic of the Faculty Hospital in Královské Vinohrady. The results were evaluated in MS Excel with a help of functions descriptive statistics and CORREL. Overall it was demonstrated that short walk tests (Up and Go, 25 feet, 10 m fast, 10 m slowly) better reflect the impact of the muscle strength, fatigue and Expanded Disability Status Scale (EDSS). Between the 6-minute test and the Paced Auditory Serial Addition Test (PASAT) was also demonstrated relatively high value of dependence.
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Optimization of the Production of Lipases by Bacillus subtilis
Slavíčková, Radka ; Omelková, Jiřina (referee) ; Hermanová, Soňa (advisor)
In this thesis, optimization of production of lipolytic enzymes by submerzed cultivation of Bacillus subtilis (BS) was studied. Production of lipolytic enzymes was tested in three nutrient media, which differed mainly in main sources of carbon, respectively of nitrogen. The first medium contained mainly extract from calf brain and beef heart (BHIB), the second medium contained peptone and yeast extract (NB) and the third one contained peptone and yeast extract with the addition of 2% (w/v) glucose (NBG). The highest lipolytic activity (0.0784 Uml-1) was measured in NBG medium. Maximum of lipolytic activity was observed before the end of the exponential phase of BS growth in all the media. Temperature optimum in NBG medium was determined from 30 to 50 °C, pH optimum in the range of 5 to 11 and subsequently the temperature stability of lipolytic enzymes produced by the BS was estimated. The activity value was determined spectrometrically using p-nitrophenyllaurate as a substrate. Produced lipolytic enzymes showed maximum activity at 37 °C in the alkaline pH of 8.0. Measurement of temperature stability showed that lipolytic enzymes are relatively thermostable enzymes retaining 100 % of the activity even after 1 hour of cultivation at 30 - 50 °C. The presence of 1% (w/v) olive oil in medium NBG caused a decrease in lipolytic activity by 65 % as well as in pH from 6.5 to 5.4 after 14 days of cultivation. After substitution of glucose by fructose in medium NBG, lipolytic activity showed comparable values during the first week of cultivation. On the other hand, the decrease of lipolytic activity by 29 % in the medium with fructose was observed after 14 days of cultivation. A procedure for the identification of lipolytic enzymes of BS by peptide massfingerprinting was developed to understand the potential of synthetic polyester - poly(e-caprolactone) as a lipase inductor. Degradation study of commercial polyester poly(e-caprolactone) was carried out by submerged cultivation of Bacillus subtilis in NBG medium at initial pH 7.0 and 30 °C for 14 days. PCL (Mn = 10,000, Mw = 14 000) was studied in the form of films (1.0 x 1.0 cm), which were prepared by melt-pressing, rapid cooling of the melt to 4 °C and evaporation of the solvent from 2 % dichlormethane solution. The evaluation of the films shown occurrence of weight loss (7.8 - 17.0 wt.%) together with the formation of numerous holes and cracks in the sample surface in relation to the method of the films preparation. Lipolytic activity values increased by 9 - 17 % in the degradation media compared to control samples. Densitometric monitoring showed also higher increase in cell mass in the degradation medium compared with control samples. Based on the results obtained, the degradation process induced by BS could be suggested.
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Pilot study of sublingual in vitro permeation of butorphanol.
Šrámková, Markéta ; Vachek, Josef (referee) ; Doležal, Pavel (advisor)
The theoretical has mentioned the basic information about pain and its treatment. Furthermore, there are described analgesics, mainly opioids. This part is specifically focused on the kinetics and pharmacodynamics of butorphanol alone. A following section deals with the basic description of sublingual administration and it provides with a description of morphology of tongue and also provides information from articles, which dealt with a similar theme as this thesis. The experimental part focuses on the permeability of a sublingual membrane using butorphanol dispersed in different vehicles. Isopropyl myristate, phosphate buffer pH 5.3, propylene glycol - water (3:2) were used. Some of the test donor samples used gelatinous membrane or chitosan donor membrane. Acceptor phase in the experiments was phosphate buffer pH 7.4 or pH 6.5. Permeation marker there was caffeine. I have found from the permeation results obtained that caffeine as a marker of choice was appropriate. Permeability tests of butorphanol brought some interesting findings on the dependence between permeability and a selected membrane type. It can be concluded from the results that the membrane permeability of the membrane affected by membrane properties more than the used vehicle.
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Electrodeposition of thin layers TiO2
Jakubis, Ivan ; Vondrák, Jiří (referee) ; Sedlaříková, Marie (advisor)
This work deals with issues of electrochromism and making active electrochromic film of titanium dioxide. By using various precursors consisting titanium element active film was electrodeposited on glass substrates covered with transparent conductive thin-film In2O3:Sn (ITO). Electrochromic characteristics of these substrates that were electrodeposited for different times and with different voltage have been studied. Than there has been studied the impact of various annealing temperatures on electrochromic characteristics.
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Portrayal of war prisoners in american media after the election of Barack Obama
Jelínek, Jiří ; Kruml, Milan (advisor) ; Jirák, Jan (referee)
The US attitude towards the prisoners of war, more accurately its media portrayal, is the main theme of this bachelor thesis. The main goal of this thesis is to find out whether this attitude in real situation corresponds with the similar attitude in the US movies and series. The real one has been changing significantly since 2001 depending on the Afghanistan and Iraq situation, and also on the decisions of the Supreme Court of the US and the people opinion. This thesis is based on official documents of the US administration as well as on several movies (24, Homeland, Zero Dark Thirty and Ghosts of Abu Ghraib). The essential thesis whether the real attitude was reflected in the movies was generally confirmed, however the differences between pictures were considerable, therefore the overall generalization is not possible.
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Valerie and Her Week of Wonders
NEKOLOVÁ, Lucie
The Bachelor's thesis deals with the interpretation of Vítězslav Nezval's book "Valérie a týden divů" and its film adaptation, which was directed by Jaromil Jireš. The aim of the thesis is to compare the individual themes and topics and consider their use in the interpretation of the book and its film adaptation. In this respect, the themes of characters and space seem particularly substantial. Therefore, the methodological chapters focus mainly on these individual aspects. Equally important seems to be the common feature of both narratives, that is, the resonance of surrealistic poetry which refers to the Czechoslovak New Wave, in which Jireš's 1970 film adaptation is also included.
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Alternative and new ways of costume design
Rybáková, Simona ; MATÁSEK, Petr (advisor) ; PTÁČKOVÁ, Věra (referee)
This work is aiming to show costume designing as an essential artistic field of today which surpasses limits of applied arts or mere decoration in an effort of emancipation.It talks about the specific characteristics of this field and how it surpasses into other domains and it opens chapters which I deemed significant to my topic. The text outlines my professional career as costume designer to my pedagogic and finally to my organisational curator?s experience. I describe how I enrich the basic practical and empirical education with an emphasis on the artistic way of expression with more theoretical and analytical abilities. The latter is documented mainly with the exhibition ?Extreme costume? in PQ 2011.An important part is the vocabulary of some rather technical terms; it is very liquid and constantly changing. Predominant tendency is to find terms commonly revealing to help the lay audience understand a discipline which can be attractive when a suitable form and presentation is used. Besides the language it is also new topics reflecting costume and its development such as; costume as performance, nudity as/and costume, event and an act of wearing a dress, installation and costume, light and costume, technology and costume which complete the perspective. The number of themes which emerged in the last 6 years is high and sometimes they are connected only seemingly. Some are entering disciplines which are interconnected and influence each other mutually. Therefore some examples of work process are used repeatedly in a comparative or illustrative role.Throughout the whole work the search is for new paths, techniques and materials for costume design and its emancipation. Next to this stands no less important role of costume as a part of action scenography as well as the postmodernism of 1980?s which is a significant starting point of my own work. In contrast to certain excessiveness stands the concept of unpretentious costume completed with specifics of film costume designing.Fashion show as a show with all the aspects of a dramatic piece/performance considers consumer and sociological topics and can be linked to reshaping and costume recycling which closely relates to economic and ecologic aspects too.Emancipation effort is joined with its presentation in public in the form of exhibitions, events, workshops and its documentation for the future. The technological ?boom? occurred especially with the emerge of light costume and ?wearables? as well as the research for materials and the work of realizers on creative ideas of the artists. An inseparable part is a dictionary of alternative materials used in costume designing and a extensive bibliography.
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Animated Video Clip in Art Education at Basic School
KALIVODOVÁ, Lucie
The thesis focuses on ways of creating an animated video clip as part of art education at basic school and on various views of art as a school subject, in which multimedia creation is used. The theoretical part describes the animated film as a specific way of intermedia expression in the context of past and contemporary art. It deals with the origin and history of animated film and describes the production and various techniques used for its creation. It analyzes the scope for the creation of an animated video clip in intermedia and experimental activities in art education at basic school. The practical part contains the implementation and reflection of my own art lessons focusing on the creation of animated video clip in class 5.B at Lerchova school in Sušice in 2013.
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Determining the plasticity of lactose
Patočková, Simona ; Řehula, Milan (advisor) ; Špaček, Vladimír (referee)
The aim of this work was to take charakteristic of lactose and their influence on compressibility. Plastic properties ensuring better compressibility of material, whereas elastic properties make difficult for compressibility. Plasticity and elasticity are important characteristics of compacting materials. Several methods are commonly used for calculation of these parameters. One of the methods utilizes elastic recovery. On the base of relation between elastic recoveries and compression pressure, the Young's modul of elasticity is counted. Elastic and plastic energy is acquired from measurement force - displacement representing continuance of pressing process. Force - displacement curves are obtained from measurements of punch force and displacement. Energy is needed for the compression of material and formation of strong compacts. The properties of the compact are correlated with the energy input. The force - displacement profiles as such may be useful as a material charakteristic. Furthermore, the stress relaxation test is exploited for calculation of plasticity. By this test the parameters F min A, F min B, FPL and also characteristics of single parametric relation dependence of compacting pressure on time are counted. In this work, the lactose was evaluated by all mentioned methods. The basic...
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