|
Employment Services for Former Substance Users in the Czech Republic in Comparison with Similar Services in Scotland and Austria
Veis, Štěpán ; Kocianová, Renata (advisor) ; Kopecký, Martin (referee)
The diploma thesis "Employment Services for Former Substance Users in the Czech Republic in Comparison with Similar Services in Scotland and Austria" discusses the problem of employment of people that used illegal drugs for some time in their lives and now abstain. To get a job and keep it is fundamental for them to be able to prosper in newly started life. Former substance users are characterized with respect to their position in the labour market and the need of social reintegration. Their employability in the Czech labour market is given from the point of view of the clients and workers of the treatment facilities, and from the point of view of potential employers. The possibilities of employment services offered to former substance users are then shown by three projects, i.e. Czech Work and Social Agency of SANANIM, o. s., Scottish Addiction Workers' Training Programme, carried out by Scottish Drugs Forum, and Austrian social firm Fix und Fertig, a branch of Verein Wiener Sozialprojekte. The projects are compared as to their approaches to the problem of employability, basic parameters that employment services offered to the former substance users should take into account are defined, and finally, the possibilities of development of these services in the Czech Republic are outlined.
|
|
Neural circuits of feeding as a potential target of the anti-obesity drugs.
Zápotocká, Hana ; Herink, Josef (advisor) ; Kudláčková, Zděnka (referee)
The problem of rising prevalence of underweight and obesity is considered to be one of the main health problem including not only western society but as well as poor states. During last twenty years a lot of work have been done for better understanding of the mechanisms involved in regulation of appetite and neuronal circuits encompassed in it. This one led to an idea of pharmacological management of this regulation and development of new potential antiobesity drugs. The approach from development leading to clinical application is a hard long-term and economically demanding task. In spite of it there are some new types of drugs waiting for approval and market introduction. This work summarizes characteristics of new potential antiobesity drugs, which may represent new target for the treatment of obesity.
|
|
Use of experimental animal models in chemotherapy induced peripheral neuropathy, possibilities for prevention and treatment
Hrzán, Lukáš ; Kubecová, Martina (advisor)
The topic of this diploma thesis is chemotherapy induced peripheral neuropathy. The first part is concerned with etiology, symptoms and diagnostics of peripheral neuropathy. The second part consists of a description of the most common antineoplastic drugs causing peripheral neuropathy. Different in vivo and in vitro models that are used in laboratory research to demonstrate the occurence of peripheral neuropathy are discussed. In the practical part, an experiment is described that was designed to evaluate whether the strain of CD1 mice can be a useful animal model to study chemotherapy induced peripheral neuropathy. Results of this experiment indicate that after 6 weeks of administration of cisplatin, paclitaxel and bortezomib, evidence of damage to the peripheral nervous system can be detected using neurobehavioral and neurophysiological tests. The final section includes information about current research in prevention and management of chemotherapy induced peripheral neuropathy. Latest recommendations for prevention and therapy of peripheral neuropathy in oncological patients are given.
|
|
ICP-OES with electrothermal vaporisation (ETV) as a possible tool for direct analysis of biologic samples
Matějková, Stanislava
Solid sampling electrothermal vaporization (ETV) inductively coupled plasma optical emission spectroscopy (ICP-OES) enable simple and rapid multielemental analysis of major, minor and trace elements without the need for pre-treatment of solid or liquid samples. By comparison with more common ICP-OES combined with a solution nebulization, the risk of contamination of analysed samples is reduced. The detection limits and the sample quantity needed for analysis via ETV-ICP-OES decrease as a result of the high-efectivity transport of the sample aerosol into the ICP torch. The connection of ETV unit to simultaneous ICP-OES allows us the fast direct and fully automatised analysis of numerous samples. The technique enable usually the combined calibration with home-made or certified solid and aqueous standards. We developed the methodology for tracking the carboran-based candidate-drugs time dependent concentration and for their pharmacokinetic evaluation. The method is based on direct boron or together boron and cobalt contents direct estimation in little amounts of fresh frozen mice tissues (5 – 10 mg of serum, brain or liver).\n
|
|
Mycotic disease - social and psychological problem
KARAFIÁTOVÁ, Iva
In my opinion, the topic of my thesis - {\clqq}Mycotic diseases {--} a social and psychological problem`` is a very current issue. In my thesis I describe individual types of mycotic diseases with regard to their epidemiological characteristics, techniques and options of treatment. I also give information on preventive measures to help prevent transmission of the infection. The first objective of the thesis was to identify the most often diagnosed mycoses in the dermatologist´s consulting room during one year. This objective was achieved by using medical history records and it was found out that the most frequent diseases are especially fungal nail infection, fungal foot infection and superficial mycosis - Pityriasis versicolor. The second objective was to evaluate psychological and social impact in patients with mycotic diseases in comparison with respondents from general public. This objective was achieved as well. Using questionnaires, 60 respondents were asked 34 questions. The questions were prepared in such a way that information both from the dermatologist´s consulting room patients and from respondents from general public could have been obtained by the inquiry. The outcome of the whole inquiry was supposed to be evaluation of two hypotheses. The first one should have confirmed or rejected the idea that patients with mycotic disease in medical history have more information on this issue, better awareness about options of transmission prevention, prevention of re-infection and they behave more responsibly regarding prevention of mycoses transmission in population than the others. The second hypothesis stated that mycotic diseases have negative social and psychological impact. In the conclusion both hypotheses were basically confirmed. Awareness of respondents from general public is not on a high level, on the contrary, large majority admitted lack of awareness about this issue. Negative impact in people suffering from this disease is very perceptible. Most respondents perceive the disease as a social handicap and they try to hide the possible visible symptoms on their body at any cost. Adverse reactions to them from their family and relatives or from the public are no exception. In my opinion, the further procedures to improve the existing situation should be adhering to preventive measures such as maintaining personal hygiene or disinfection of risky areas. However, I find especially important to talk about these diseases, in public and without constraints. To support the diseased and to inform general public by means of media, leaflets or by public lectures and talks.
|
|
Mass Spectrometry Imaging of Metformin in Tissue Sections
Strnad, Štěpán ; Sýkora, D. ; Vrkoslav, Vladimír ; Cvačka, Josef ; Maletínská, Lenka ; Pirník, Zdenko
Mass spectrometry imaging is a powerful technique suitable for visualization of the distribution of a wide variety of compounds within tissue sections. The main aim of the study was the development and optimization of a sample preparation procedure allowing determination of the distribution of orally dosed metformin in mice kidney sections. Metformin is the first-line medication for the treatment of type 2 diabetes. The optimization of the sample preparation step before imaging experiments included the selection of a suitable matrix and the optimization of various parameters of MALDI analysis. 2,5-dihydroxybenzoic acid was identified as the best matrix providing highest sensitivity. A sublimation method was successfully used for the matrix deposition. The highest relative concentration of metformin was found in the inner zone of kidney 30 minutes after the drug administration.
|
|
Gene Therapy of CML: Experimental Vaccines against Bcr-abl-transformed Cells
Lučanský, Vincent ; Vonka, Vladimír (advisor) ; Roubalová, Kateřina (referee) ; Reiniš, Milan (referee)
Chronic myeloid leukemia is malignant disease characterized by myeloproliferative clonal expansion of hematopoietic stem cell. It is causally associated with the formation of the so called Philadelphia chromosome and production of its specific product, the chimeric BCR-ABL protein. The amino acid sequence of the fusion region is unique, implying that the BCR-ABL protein carries tumor specific antigen. Currently imatinib mesylate dominates the treatment of CML. It is well tolerated and when compared to the other drugs used, it prolongs the life expectancy significantly. Unfortunately, it is not capable to cure the disease. The only potentially curative approach nowadays is the bone marrow transplantation; however, it is connected with a relatively high morbidity and mortality. Moreover, it is available only to a minority of the patients. Under these circumstances the need for the development of a relatively safe and generally available treatment is understandable. Immunotherapy could be such a treatment. Several experimental vaccines based on BCR-ABL sequence were developed and tested in mice in our institute. The DNA vaccines used were carrying sequences coding for the whole BCR-ABL protein, or for 25 amino acids long junction region (these DNA sequences were fused with adjuvant genes such as...
|
| |
|
Configuration of factors influencing the formation of addiction in adolescents
Brotánková, Tereza
This thesis concerns the problems of the juvenile drug-addicts, who were ordered preliminary ruling or undertaking in-patient treatment by the Court. The object of the thesis involves realization of the configuration of the factors, which has led to drug abuse by six juvenile persons. Furthermore, it points out the possibilities and risks of intervention from the state authorities and institutions in case of occurance of behaviour disorder and negative phenomenons of the social development of a child. From the methodological point of view the matter concerns the qualitative approach whereby the methods of gaining of the data have involved interviews, work with the documents and observation. The data have been analyzed by means of descriptive approach, the method of creating of the tufts and the method of retrieval and distinguishing of the relationships. The interpretation has run in three phases. The first phase involved processing of the collected data in order to distinguish the basic categories and subcategories of the analysis, during the second phase the whole life-experience of the juvenile drugaddicts in the form of the case study has been performed, during the third phase the case studies are being discussed with the existing "Profiles" of the drugaddicts. Powered by TCPDF (www.tcpdf.org)
|
|
název v anglickém jazyce není uveden
Michalská, Dana ; Štěpán, Jan (advisor) ; Stárka, Luboslav (referee) ; Pavelka, Karel (referee)
Microdamage of bone tissue is one of important aspects of quality of bone. Marked suppression of bone turnover by bisphosphonates is associated with increased accumulation of bone microdamage in animal models. The purpose of our study was to test the hypothesis that long-term treatment with alendronate (ALN) results in accumulation of microdamage in bone in women after menopause and in that case to impairment of bone quality. In this cross-sectional study, 66 women with postmenopausal osteoporosis (mean age of 68.0 years, mean BMD T-score of - 1.7 at total hip and -2.8 at lumbar spine; 62% with prevalent fractures) were evaluated. Thirtyeight had been treated previously with ALN (10 mg/day for a mean duration of 63.6 months) while 28 were treatment naive (TN). Before initiation of ALN treatment, mean serum PINP, plasma CTX, and OC were significantly increased as compared with reference values in premenopausal women (P< 0.05). After 6 months of ALN treatment, the mean of biochemical bone markers indicated significant suppression of bone remodeling (-70.5%, -84.1%, and - 67.3% for PINP, CTX and OC, respectively; P< 0.05). After 43 months of ALN treatment, markers of degradation and synthesis of type 1 collagen were below the lower limit of normal reference range in 50% of women treated with ALN. Without...
|
guest ::
