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Subdeprivation and its consequences for a school development of the child
KOLÁŘOVÁ, Kateřina
My final work recognizes the syndrom known as psychological deprivation and subdeprivation. The theoretical part is aimed at defining the concept of psychological deprivation and subdeprivation and then focused on professor´s Matějček research, on whose methods and results is based the whole work. The empirical part of the research deals with a specific degree of psychological deprivation and subdeprivation in the constitutional institutions and families, and its detailed analysis.
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The Czech - German dialog at life and writings of nationalist, priest and educator František Pravda
BÍLKOVÁ, Martina
Submitted diploma thesis describes the life of priest František Pravda, born Vojtěch Hlinka. He was well-known writer and founder of short stories with rural theme. The thesis introduces Hlinka as a writer, but primarily as a man of many other professions and interests. The way of life brought him to Hrádek u Sušice to work for an aristocratic family of Sturmfeders. Attention is concerned with mutual relations between the Czech patriot and four generations of German baronial family. Hlinka worked as an educator of feeble-minded Ottokar Sturmfeder in Hrádek´s castle. His extraordinary psychopaedics talent is compared with usual provincial and European contemporary standards. There is also depicted Hlinka´s political, writing and religion career and his other interests. The thesis doesn´t overlook Hlinka´s contacts with important representatives of artistic and political life, which helps to complete the picture of Hlinka´s personality.
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Adolf Liebscher 1857-1919
Dlábková, Markéta ; Slavická, Milena (referee) ; Prahl, Roman (advisor)
Adolf Liebscher belongs among the artists known as the "National Theatre Generation." Like ist foremost representatiwes, F. Ženíšek, M. Aleš and V. Hynais, Liebscher also produced an oeuvre of great diversity - he created decorative paintings and historical compositions, was interested in folklore painting, his drawings were published by the major journals of that time as Zlatá Praha or Světozor and rus illustrations appeared in many albums, books of poems and novels. Liebscher was bom on March 11, 1857, into a family of a Provincial Committee engineer Josef Liebscher. His older brother Karel (1851 - 1906) later became an appreciated landscape painter, with whom Adolf often cooperated. In 1875 - 1878, Adolf Liebscher studied at the School of Applied Arts in Vienna, in the three-year course for hand-drawing teachers under F. Laufberger. After obtaining a diploma, he entered the studio of E. A. Donadini for half-a-year, where he prepared his proposals for the competition for the National Theatre. In 1879, he was awarded in the competition and was commissioned to carry out some of his proposals in the passage of the theatre. Here he created eight lunette allegories of Dramatic arts.
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Marketing and communication strategy of BERNARD Brewery
Dokulilová, Vlasta ; Postler, Milan (advisor) ; Mikeš, Jiří (referee)
The bachelor thesis deals with the analysis of marketing and communication strategy of the Bernard Family Brewery. The aim is to assess the current strategy and to propose practical recommendations for the future. The theoretical part of the thesis is focused on the basic concepts of marketing, creating marketing and communication strategy, using well-known tools and methods. In the practical part is presented the analyzed company and its product portfolio. The thesis is based on the evaluation of the current use and application of marketing and communication strategy. In conclusion, on the basis of evaluated data, are made corresponding recommendations and possible changes.
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Insight into the structure of tetrameric flavoprotein WrbA involved in oxidative-stress response
WOLFOVÁ, Julie
This Ph.D. thesis addresses the structural characterization of the unique family of tetrameric flavoproteins WrbA, the role of which in the life of cells is still largely unknown but its enzymatic activity and expression properties implicate it in the cell protection against oxidative stress. Proteins of the WrbA family were proved to carry out two-electron reductions of quinones and in this way to prevent generation of the free radicals, similarly to other flavoproteins known as quinone oxidoreductases. Crystal structures of the liganded and unliganded forms of the prototypical WrbA from Escherichia coli were determined. Comparative analyses of these structures with the related flavoproteins were intended to identify and explain the defining structural features of the WrbA family and to clarify its structural and functional relationships to the other flavoproteins.
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Cold adaptation of insects: the role of shock proteins from Hsp family.
ŠTĚTINA, Tomáš
Heat shock proteins (Hsps) form evolutionarily conserved group of proteins that are best known for their unique function in repair of miss folded proteins that would otherwise aggregate in cells during stress conditions. The most extensively studied is the inducible Hsp70. Using 3rd instar larvae of transgene mutant of fruit fly, Drosophila melanogaster that lacks all copies of hsp70 gene, we assessed: 1) whether the absence of this gene is deleterious for larvae in their capacity to survive cold stress; and 2) whether any other gene from Hsps family may compensate for the loss of hsp70. We found that 1) survival of cold stress is not altered in Hsp- larvae when compared to wild type Hsp+ larvae; 2) there is no other gene of Hsps family that would compensate for the loss of hsp70 at the level of transcription. Our ongoing effort is focusing on analysis of cold stress response at the protein level.
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Energy sensitive X-ray radiography for the non-destructive inspection of historical paintings
Žemlička, J. ; Jakůbek, J. ; Hradil, David ; Hradilová, J. ; Kroupa, M. ; Mislerová, H.
The technical composition of painted artworks is usually very complex and they belong to the most sophisticated cultural heritage artefacts. In the field of their inspection there is a rising demand for the non-destructive imaging and analytical methods which are able to reveal the inner composition of investigated objects. Several non-invasive methods based on the interaction of ionizing radiation with the matter have been successfully utilized during the last decades. These methods can be divided into two main groups. The better known are transmission methods (e.g. classical X-ray radiography) the less-used are emission methods (e.g. X-ray fluorescence imaging). The quality of the obtained image is highly dependent on the imaging characteristics of the used detector. The presently used ones (CCD cameras and CMOS sensors) create the image from analogue signal by the charge integration. This image is usually degraded by the presence of noise. This complication is exceeded by novel pixel detectors of Medipix family based on single particle digital counting. Furthermore these devices offer very high contrast (in principle unlimited) in the obtained image. The image can be acquired with spatial resolution better than one micrometer. Another advantage of these detectors is their ability to directly measure the energy of incident particles. This feature can be used for energy sensitive X-ray radiography (i.e. multi-channel images) and X-ray fluorescence mapping of the surface elemental composition. For the purposes of this work the laboratory ALMA in Prague prepared several multilayer samples of paints using different pigments. The results of mentioned methods applied on the test samples are summarized in this article. The first goal of these measurements is to build a comprehensive methodology for the application of these procedures in the laboratory.
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Molecular genetic and biochemical studies of selected inherited metabolic disorders, development and applications of new methods
Mušálková, Dita ; Hřebíček, Martin (advisor) ; Adam, Tomáš (referee) ; Macek, Milan (referee)
Inherited metabolic disorders (IMD) form a diverse group of several hundred different diseases with a relatively high cumulative incidence (stated up to 1:600). They are associated with accumulation of the substrates and lack of the products in specific metabolic pathways, which is caused by deficiency of the enzyme or its activator, or dysfunction of the transport protein. However, the underlying cause is at the DNA level. The grounds for different phenotype manifestation in patients with the same genotype are often not known. During my work at the Institute of Inherited Metabolic Disorders, I designed several new methods for the research of IMD and applied them in the patients and their families. I created procedures for the isolation of lysosomal membranes that are used for the research of lysosomal storage disorders and general properties of lysosomes. Next, I introduced several novel assays for determination of the X-inactivation ratio, which led to a significant increase of informative women. Nowadays, we use these methods in heterozygous women with X-linked diseases in order to study the influence of X-inactivation on the manifestation of the diseases. The cases of a girl with mucopolysaccharidosis type II, a girl with OTC deficiency and a family with the mutation in HPRT1 gene are described...
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Business environment in the Czech Republic
Sekuličová, Dominika ; Petříček, Václav (advisor) ; Bolotov, Ilya (referee)
The thesis deals with the quality of business environment of the Czech Republic, nevertheless, the main goal of the thesis is to evaluate the current system of the export support. Firstly, the opening chapter attempts to define the term business environment theoretically, after that it puts forward the possibilities of quantifying the quality of this indicator and then summarizes its evolution specifically in the Czech Republic. The second chapter is particularly devoted to the system of economic diplomacy in the Czech Republic and concentrates primarily on the support of export as a part of national business support programs. In this context, the thesis analyses in full detail the Export Strategy of the Czech Republic for the period 2012-2020, its updated version of 2016 and also how defined goals are met on an ongoing basis. The final part provides the export survey carried out within small and medium-sized enterprises. This survey evaluates whether the system of the state export support is generally known and used within this segment.
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The importance of Actinobacteria in Arctic soil
BOŠKOVÁ, Hana
This work is aimed for Actinobacteria and describes their importance in Arctic soil. The members of Actinobacteria are known for their ability to decompose complex natural biopolymers and because they are able to live in harsh arctic environment they could play there an important role in organic matter decomposition. The work compares their abundance in different soil horizons with the focus on cryoturbations and determines the influence of temperature on their amount. This work also represents the results of testing pure Actinobacterial isolates for the production of cellulolytic enzymes.
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