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Single cell expression analysis of genes with potential mrna gradient in mouse oocytes
Dorosh, Andriy ; Margaryan, Hasmik ; Vodička, Martin ; Ergang, Peter ; Šídová, Monika ; Dvořáková-Hortová, Kateřina
In frogs, there are clearly visible differently pigmented animal and vegetal poles of the egg determined before fertilization and leading to asymmetrical divisions. Mammalian egg does not show any comparable differentiation and it has been generally accepted that even the individual blastomeres in 2-cell and 4-cell embryos are homogenous. However, recent findings suggest that those blastomeres display different gene expression patterns and might already possess some inclinations to specific cell lineages. We therefore raised a question, whether there could be any mRNA or protein gradients in pre-fertilization oocytes similar to a previously described amphibian egg one. In mammalian eggs, there is a membrane region that is poor in microvilli, cortical granules are absent beneath plasma membrane and sperm cells generally do not bind to this location. This microvilli free region also covers the egg nucleus, and cytoskeleton localization differs markedly to the rest of the cortical space, forming actin –myosin II cortical cap/ring and is considered as animal pole. The purpose of this study was to determine gene products that can be detected at single cell level using qPCR and display gradient like distribution in mature oocytes. We checked expression of 12 selected genes in a pool of 10 oocytes and single mature oocytes. Then, we analysed gene expression in fixed intact oocytes and those undergoing laser capture microdissection procedure (LCMD). Eventually, we have determined six candidate genes for the study of intracellular spatial gene expression in mature mammalian oocytes by subcellular qPCR and in situ hybridization.
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Effect of a graduate of field of study Special Education - tutorship in practice of providers of social care
SALABOVÁ, Jana
Theoretical part of the thesis is divided into 4 main chapters. First one deals with the helping professions generally, their definitions and definition of the Special education - Tutorship branch of study per se.It also briefly touches the subject of readiness of the future graduates to deal effectivelly with the challenges of a career in the field of social services. Chapter number two is called Special education and it defines the subject of special education itself, it's purpose and inner structure. Third chapter focuses on social services. It brings up it's definition and types and forms of social services.It also contains the examples of the institutions which fall into this category and it's providers. Chapter number four, which happens to be the last one in the theoretical part of the thesis, covers the role and purpose of graduates of the special education - tutorship studies. It brings up the definition of a social worker, necessary prerequisites as well as beneficial personality traits and feautures. In a subchapter of chapter four is a short description of a pedagogue tutor. Definition who he actually is, who are proper candidates for this position, what are desired personality traits we look for in tutors and the actual content and purpose of their work in institutions providing social services. The end of chapter four is dedicated to a position of a social worker. His activities, the position and definition of a personal assistant and again personality traits needed for a successful career in this field. The objective of the practical part of the thesis is to describe working of Special education - tutorship studies graduates in practice. Particularly their experiences "from the field", how do they evaluate the level of skill of fresh graduates during their first encounters with the reality, how satisfied are they with their choice of a career so far and what was their motivation to pursue such career. Practical part of the thesis includes qualitative research. To meet the above objectives, data collection technique had been chosen using a structured interview with the respondents, who were graduates of Special education tutorship studies. You can tell by the results of this thesis that graduates in the field of social services apply mostly to lower positions as a social service workers, even though they are qualified to perform as a social workers. The age of clients with whom the respondents work ranges from childhood to senior. The motivation that led those questioned respondents to the decision to choose the area of social services varies. Some reported that they were influenced by the actual practical experience during their studies at the university, when both the work itself and their enthusiasm for the target group played role. Others expressed they had previous experience working with people with disabilities or they just wanted to acquire new experiences. Also, involvement of a family member and their previous education was a motivation. Job satisfaction is evident from observed data, and it also contributes to work done accurately and responsibly. Personal benefits for the respondents according to findings are usually their usefulness at work, new experiences they are getting and last but not least advances of their clients. As the biggest advantage the respondents usually mention variability of their work, target group and the clients in itself and the fact, that their work is highly satisfying and they basically work in a very pleasant team of individuals. On the other hand, not exactly satisfying financial reward and challenging working hours are mentioned as disadvantages. Special education - tutorship studies - the branch they've studied, is by the majority of respondents considered to be beneficial for them and they especially highlight practical impact of practice they completed during their studies and which included visits to various relevant institutions.
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Supervision in psychiatric nursing
RŮŽIČKOVÁ NEUŽILOVÁ, Michaela
Basic theoretical background The theoretical part of the thesis explores the issue of supervision and focuses on psychiatric nursing. It is divided into several parts. The first part is focused on supervision, briefly characterizes supervision as such, then it describes its divisions, functions and methods. It also explores the participants of supervision, the agreement on supervision, transcultural supervision and Balint groups. The following part describes historical development of psychiatric nursing, briefly presents the personality of Joyce E. Travelbee. In the conclusion of the theoretical part of the thesis the problems of education and competence of nurses in psychiatric care are introduced, marginally mentioning the Turku declaration. The aim of the work The main aim of the thesis is to investigate the awareness of nurses working in psychiatric care of the clinical supervision options. The task of the second objective is to find out the opinion on supervision of nurses working in psychiatric care. The third objective is focused on means to assist nurses working in psychiatric care in their personal development and growth within their practices. The purpose of the fourth objective is to find experience with supervision of nurses working in psychiatric care. The methods used Qualitative investigation was chosen for the empirical part of the thesis. The method used for data collection was in the form of semi-structured interview. The interviews with the respondents were taken down using a voice recorder and transcribed, qualitative data was then analyzed by pencil and paper encoding technique and semantically organized into 27. groups of categorization. The selection of a research sample was intentional, consisting of twelve nurses working in psychiatric care in the Czech Republic. Results The research focused on nurses who do not have personal experience with supervision revealed that they have certain awareness of the supervisory process, however not quite sufficient to be able to describe the nature and function of supervision in its entirety. Their knowledge is rather theoretical. They understood supervision as help to solve disharmonious relationships in the workplace, as well as it could improve communication skills of the nurses, it would also assist in dealing with crisis intervention and prevent burnout syndrome. The results regarding nurses who do not participate in the supervisory process clearly indicated that they have insufficient support and the opportunity for both their professional and personal growth in their workplace, while this important could be solved by supervision. The research, which covered only nurses who have had personal experience with supervision and regularly participate in it, showed that their knowledge and skills related to the supervision process are quite sufficient to be able to understand and use supervision practically in the whole range. These nurses sees supervision as a benefit that would allow them not only to understood themselves, but also to learn how to manage challenging situations that are a part of their profession. Supervision gives them confidence, provides support and teaches them to deal with people who are part of their work process. Supervision has become a tool for preventing burnout and showed them how to use their own potential effectively. Conclusion This thesis is focused on the use of supervision in psychiatric nursing. Working with mentally challenged patients is very demanding, therefore supervision plays its justified role there. If you do not know yourself and you are not able to solve your own problems, you can never effectively and comprehensively address the problems of others, who are often desperately waiting for your help. The results will be presented at professional seminars designed not only to nurses working in psychiatric care.
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Marketing communication in the selected company
Kotrč, David ; Štůsek, Jaromír (advisor) ; Dagmar, Dagmar (referee)
This Master thesis covers the marketing communications. The main aim of the work is to draft recommendations that will improve marketing communications of CENTRA a. s. The first part of master thesis is focused on the theoretical basics which explains the concept of marketing, marketing mix, marketing communication, communication mix, communication on the Internet, B2B communications and CRM systems. In the second practical part is processed analysis of the marketing mix of the company and analysis of marketing tools used by CENTRA a. s. After processing analysis of the marketing mix and analysis of marketing tools was a proposed recommendation for improvements of marketing communications of CENTRA a. s.
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Tool to assess the landscape connectivity in different area units and dispersal distances
Chrumko, Adam ; Šímová, Petra (advisor) ; Krčílková, Šárka (referee)
The purpose of this diploma thesis is to introduce a basic summary of landscape connectivity calculations mainly focusing on the Conefor program and the effect of input parameters on output landscape connectivity indices. One of the main goals is to create an ArcMap Toolbox to simplify the calculation of landscape connectivity indices for many areas of interest. Basic squares that cover the whole Czech Republic were chosen as main areas of interest. These units are used for example for mapping the abundance of flora and fauna. The same indices were calculated for NUTS3 and LAU1 areas in order to compare effect of size of input areas. This thesis provides a simple solution in the form of a Python Toolbox for Esri ArcMap to calculate overall indices of landscape connectivity through a Conefor command line application for a large number of input areas. Forest connectivity for different dispersal distances and input areas of interest was calculated with this Toolbox. As an input vector layer of forests, CORINE land cover data from mapping period 1990, 2000, 2006 and 2012 were used. Input dispersal distances were chosen in the range from 50 to 50.000 meters in order to comprehensively describe their effects on indices. Resultant forest and connectivity changes are represented by cartograms; the effect of input data on output indices is shown in graphs.
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Provision of Equine Assisted Activities and Therapies and the biggest organizations in Czech Republic and Slovak Republic
Jindříšková, Jana ; Doležalová, Jana (advisor)
The main goal of this bachelor thesis is to define conditions for providing the ill with Equine Assisted Activities and Therapies. In this way the readers can learn a definition of Equine Assisted Activities and Therapies, what education and experience therapists who want to implement this practice should have and what requirements should a horse meet to be used in the therapy. In the next part I do research on coverage of Equine Assisted Activities and Therapies expenses by insurance companies not only in Czech, but also in Slovak Republic. The research also includes answers to the questions, what criteria should a patient meet to get hippotherapy care covered and what attitude to such care the insurance companies have. Besides problems in relations insurance company, patient and centre of Equine Assisted Activities and Therapies, in my thesis I describe the biggest centers of Equine Assisted Activities and Therapies in Czech Republic and Slovak Republic.
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Comparison of Antivirus Software
Papica, Jiří ; Havránek, Martin (advisor) ; Benda, Petr (referee)
This bachelor thesis focuses on comparison of selected anti-virus programs and subsequent choosing of the most suitable one for given computer.
The thesis consists of two major parts. The first part focuses on anti-viruses and malware, which are divided into various types. In addition to the anti-virus section there are topics which are covering things like anti-virus tools and mechanism, e.g. firewalls and heuristics. At the end of the theoretic part there are topics which are briefly touched upon, topics like hardware basics of every computer which are later on important for the actual testing parts.
The second major part of this thesis is about choosing the right attributes for ani-viruses, about choosing the right criteria for their future evaluation and finally the measurement of said criteria. The criteria that were chosen are thus: price, time it takes for video to be rendered in Sony Vegas Pro, time it takes for GPU benchmark to finish, time it takes for CPU benchmark to finish and finally the last criteria that was picked is the effectiveness against real-life threats. Anti-virus programs that were being evaluated are Eset Smart Security 9, Norton Security Deluxe, Avast! Internet Security and lastly Kaspersky Anti-Virus. The recorded values were converted into a point-based system, which allowed for clear and evident illustrations via graphs and charts. From the final graphs and charts I was able to recommend the most suitable anti-virus for given computer.
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Activities and competencies of the Customs Administration of the Czech Republic within protection of endangered plants and animals
Bašta, Lukáš ; Vojar, Jiří (advisor) ; Solský, Milič (referee)
The Bachelor thesis deals with the issue of activities and competencies of the Customs
Administration of the Czech Republic as armed security force within protection of
endangered plants and animals. Bachelor thesis introduces international trade in
endangered plants and animals, legislative protection options (CITES Treaty, EU/ES law
and law of the Czech Republic) and its application in the Czech Republic, the specific
ways and means of the Customs Administration of the Czech Republic in order to detect
illegal activities, presently - an overview of discovered illegal activities by Customs
Administration of the Czech Republic for the years 2011 - 2015, assessment of trends
and developments in the context of smuggling endangered species, most smuggled
species, offenders, methods, covers, penalties, draft of prevention tools and evaluation of
the current situation.
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Change trajectories of wood-pastures focusing on changes in management
Čermáková, Kamila ; Skaloš, Jan (advisor) ; Pavel, Pavel (referee)
This thesis is focused on monitoring changes in the trajectory of wood-pasture mainly in changes in management. The main goal of research was land-cover according to the Imperial prints and current orthophoto maps. Area of interest is located in the Stredocesky and the Ustecky region, specifically my intereset was six historic regions in the Midwestern Bohemia, which had the largest landscape heterogeneity.
The first part describes the historical development of wood-pastures, their definition and the current status. Information was obtained from the Czech and foreign literature. Another part deals with specific trajectories in the area of interest. Changes in wood-pasture from mid-19th century to the present day were compared by program ArcGIS. In conclusion you can find the evaluation of the results by graphs, tables and verbal evaluation of the likely causes of changes in trajectory.
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Changes in the development of forest and non-forest woody vegetation at the landscape level - case study (the former estate of Decin)
Čermáková, Eva ; Skaloš, Jan (advisor) ; Pavel, Pavel (referee)
This masters thesis deals with the longterm changes of forest and nonforest woody vegetation in the area of the former Děčín estate. For my analysis I have used the avalaible historical maps and modern ortophotographs, which I have processed using the ArcGIS software.
While many places of the Earth are going through deforesation, on the other hand in Europe there is a significant rise in the area covered by woodlands. I have come to the same results in my thesis, in which at the studied site, in which there was an expansion of forest woody vegetation between the years 1953 and 2015. While when comparing the map of The Stabile cadaster and an aerial image from 1953 there was a decrease of forest woody vegetation. These results pary correlate with the demographic development of the Děčín district as well as general political and economic development of the area in the analyzed time period. The results are compared to similar avalaible studies, on the base of which it seems that the resukts of this study are not a unique trend.
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