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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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The use of biomass ash
Ochecová, Pavla ; Tlustoš, Pavel (advisor) ; Radim, Radim (referee)
One of the most frequently used sources of renewable energy is biomass, mainly wood biomass. Incineration is the most common technology utilizing the energy from biomass to produce heat. A byproduct of these technologies is ash, whose composition depends on the feedstock composition and the incineration technologies. Due to the high content of valuable nutrients in ash, one of the suitable option for ash utilization seems to be application into agricultural or forestry land. Therefore, it is necessary to test response of soils and plants and look for the most suitable combination of soil additive (biomass ash), and plants. Experimental part of PhD thesis was divided into the incubation experiment and the vegetation pot experiments. The incubation experiment: The efficiency of ash addition at two application rates was tested for nutrients enrichment in different soils within period of 56 days. The vegetation pot experiments: The two plants Triticum aestivum L: (three-year experiment) and Lolium perenne L. (one-year experiment) were planted in the 5L pots. Soils were treated with ash or ash combinated by superphoshate and flue gas desulfurization gypsum. For both plants, we evaluated the plant growth, biomass production and content of macro, micro and toxic elements in the biomass and in the soils.
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Design and implementation of e-learning in the area of interest
Marešová, Nikola ; Husa, Jiří (advisor) ; Marek, Marek (referee)
The bachelor's thesis entitled "Design and implementation of e-learning in the area of interest" deals with buying used cars. The essential part of this work is a creation of e-learning course for buyers and other people interested in this area which aims to improve their knowledge. The participants will get an overview of the most common situations and problems they can meet when buying a used car. The theoretical part presents an overview of the topics of the issue of purchase of used cars. It may serve as a theoretical guidance to the e-course, it brings its content. The topics are based on the analysis of information from printed and electronic sources, supplemented by experiences of the author and experts in the field. The practical part deals with the creation of an e-learning course. It contains an analysis of the needs of buyers, the reasons for the creation of the course and the requirements of it, its objectives, a description of the used authoring tool eXe and graphic creation process from the creation of the course after its publication in steps. All this is complemented by the feedback from six volunteers who have expressed an interest in testing the course and then doing a review. Before the creation of the course it was necessary to analyze the situation and find out why such a course is needed. The acquired knowledge was then processed in the course by the synthesis method. For the evaluation method it was used a short personal interview where the author examined satisfaction with the course, its possible shortcomings and suggestions for improvements or enhancements.
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Partial Report IV / 2016 - Rating of the deformation stress monitoring the state of the rock mass during mining coal seam 30 (634) within the trial operation of mining method corridor - a pillar in OPJ ČSM - NORTH
Waclawik, Petr ; Ptáček, Jiří ; Kukutsch, Radovan ; Kajzar, Vlastimil ; Koníček, Petr ; Souček, Kamil ; Staš, Lubomír
Monitoring of the deformation stress state of the rock mass is a prerequisite for the verification of unapproved new mining methods hall-pillar and its further application in the Czech part of the Upper Silesian coal basin. This mining method is designed on the basis of experiences and practices that are verified in different natural conditions and depths below the surface and is therefore essential for the verification of conditions for the Czech part of the Upper Silesian Basin based on geotechnical monitoring. The present report is prepared on the basis of a contract no. 942/50/10, where the Institute of Geonics, v.v agrees to make periodic evaluation of monitoring data napěťodeformačního state of the rock mass. In accordance with the aforementioned agreement, the message is processed in the six-month period and continues in the interim report III / 2015 (Waclawik et al. 2015) passed buyers in April this year. Interim results of the geotechnical monitoring, such as the experience gained during the first conquest dobývky V show specifics of natural conditions in trial operation unapproved new mining methods hall-pillar.
Fulltext: content.csg -  PDF
Plný tet: UGN_0464907 - PDF
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(100) substrate processing optimization for fabrication of smooth boron doped epitaxial diamond layer by PE CVD
Mortet, Vincent ; Fekete, Ladislav ; Ashcheulov, Petr ; Taylor, Andrew ; Hubík, Pavel ; Trémouilles, D. ; Bedel-Pereira, E.
Boron doped diamond layers were grown in an SEKI AX5010 microwave plasma enhanced chemical vapour deposition system. Effect of surface preparation, i.e. polishing and O2/H2 plasma etching on epitaxial growth on type Ib (100) HPHT synthetic diamonds were investigated. Using optimized substrate preparation, smooth (RRMS ~ 1 nm) boron doped diamond layers with metallic conduction and free of un-epitaxial crystallites were grown with a relatively high growth rate of 3.7 μm/h. Diamond were characterized by optical microscopy, optical profilometry, atomic force microscopy and Hall effect.
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New Methods for Increasing Efficiency and Speed of Functional Verification
Zachariášová, Marcela ; Dohnal, Jan (referee) ; Steininger, Andreas (referee) ; Kotásek, Zdeněk (advisor)
Při vývoji současných číslicových systémů, např. vestavěných systému a počítačového hardware, je nutné hledat postupy, jak zvýšit jejich spolehlivost. Jednou z možností je zvyšování efektivity a rychlosti verifikačních procesů, které se provádějí v raných fázích návrhu. V této dizertační práci se pozornost věnuje verifikačnímu přístupu s názvem funkční verifikace. Je identifikováno několik výzev a problému týkajících se efektivity a rychlosti funkční verifikace a ty jsou následně řešeny v cílech dizertační práce. První cíl se zaměřuje na redukci simulačního času v průběhu verifikace komplexních systémů. Důvodem je, že simulace inherentně paralelního hardwarového systému trvá velmi dlouho v porovnání s během v skutečném hardware. Je proto navrhnuta optimalizační technika, která umisťuje verifikovaný systém do FPGA akcelerátoru, zatím co část verifikačního prostředí stále běží v simulaci. Tímto přemístěním je možné výrazně zredukovat simulační režii. Druhý cíl se zabývá ručně připravovanými verifikačními prostředími, která představují výrazné omezení ve verifikační produktivitě. Tato režie však není nutná, protože většina verifikačních prostředí má velice podobnou strukturu, jelikož využívají komponenty standardních verifikačních metodik. Tyto komponenty se jen upravují s ohledem na verifikovaný systém. Proto druhá optimalizační technika analyzuje popis systému na vyšší úrovni abstrakce a automatizuje tvorbu verifikačních prostředí tím, že je automaticky generuje z tohoto vysoko-úrovňového popisu. Třetí cíl zkoumá, jak je možné docílit úplnost verifikace pomocí inteligentní automatizace. Úplnost verifikace se typicky měří pomocí různých metrik pokrytí a verifikace je ukončena, když je dosažena právě vysoká úroveň pokrytí. Proto je navržena třetí optimalizační technika, která řídí generování vstupů pro verifikovaný systém tak, aby tyto vstupy aktivovali současně co nejvíc bodů pokrytí a aby byla rychlost konvergence k maximálnímu pokrytí co nejvyšší. Jako hlavní optimalizační prostředek se používá genetický algoritmus, který je přizpůsoben pro funkční verifikaci a jeho parametry jsou vyladěny pro tuto doménu. Běží na pozadí verifikačního procesu, analyzuje dosažené pokrytí a na základě toho dynamicky upravuje omezující podmínky pro generátor vstupů. Tyto podmínky jsou reprezentovány pravděpodobnostmi, které určují výběr vhodných hodnot ze vstupní domény. Čtvrtý cíl diskutuje, zda je možné znovu použít vstupy z funkční verifikace pro účely regresního testování a optimalizovat je tak, aby byla rychlost testování co nejvyšší. Ve funkční verifikaci je totiž běžné, že vstupy jsou značně redundantní, jelikož jsou produkovány generátorem. Pro regresní testy ale tato redundance není potřebná a proto může být eliminována. Zároveň je ale nutné dbát na to, aby úroveň pokrytí dosáhnutá optimalizovanou sadou byla stejná, jako u té původní. Čtvrtá optimalizační technika toto reflektuje a opět používá genetický algoritmus jako optimalizační prostředek. Tentokrát ale není integrován do procesu verifikace, ale je použit až po její ukončení. Velmi rychle odstraňuje redundanci z původní sady vstupů a výsledná doba simulace je tak značně optimalizována.
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Acceleration of Object Detection Using Classifiers
Juránek, Roman ; Kälviäinen, Heikki (referee) ; Sojka, Eduard (referee) ; Zemčík, Pavel (advisor)
Detekce objektů v počítačovém vidění je složítá úloha. Velmi populární a rozšířená metoda pro detekci je využití statistických klasifikátorů a skenovacích oken. Pro učení kalsifikátorů se často používá algoritmus AdaBoost (nebo jeho modifikace), protože dosahuje vysoké úspěšnosti detekce, nízkého počtu chybných detekcí a je vhodný pro detekci v reálném čase. Implementaci detekce objektů je možné provést různými způsoby a lze využít vlastnosti konkrétní architektury, pro urychlení detekce. Pro akceleraci je možné využít grafické procesory, vícejádrové architektury, SIMD instrukce, nebo programovatelný hardware. Tato práce představuje metodu optimalizace, která vylepšuje výkon detekce objektů s ohledem na cenovou funkci zadanou uživatelem. Metoda rozděluje předem natrénovaný klasifikátor do několika různých implementací, tak aby celková cena klasifikace byla minimalizována. Metoda je verifikována na základním experimentu, kdy je klasifikátor rozdělen do předzpracovací jednotku v FPGA a do jednotky ve standardním PC.
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Security of Biometric Systems
Lodrová, Dana ; Busch, Christoph (referee) ; Provazník, Ivo (referee) ; Drahanský, Martin (advisor)
Hlavním přínosem této práce jsou dva nové přístupy pro zvýšení bezpečnosti biometrických systémů založených na rozpoznávání podle otisků prstů. První přístup je z oblasti testování živosti a znemožňuje použití různých typů falešných otisků prstů a jiných metod oklamání senzoru v průběhu procesu snímání otisků. Tento patentovaný přístup je založen na změně barvy a šířky papilárních linií vlivem přitlačení prstu na skleněný podklad. Výsledná jednotka pro testování živosti může být integrována do optických senzorů. Druhý přístup je z oblasti standardizace a zvyšuje bezpečnost a interoperabilitu procesů extrakce markantů a porovnání. Pro tyto účely jsem vytvořila metodologii, která stanovuje míry sémantické shody pro extraktory markantů otisků prstů. Markanty nalezené testovanými extraktory jsou porovnávány oproti Ground-Truth markantům získaným pomocí shlukování dat poskytnutých daktyloskopickými experty. Tato navrhovaná metodologie je zahrnuta v navrhovaném dodatku k normě ISO/IEC 29109-2 (Amd. 2 WD4).
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Ratings business accounts
ŠUSTROVÁ, Lucie
The diploma thesis is focused on using methods of multiple criteria decision making, in this case about business accounts. In theoretical part is described the banking systém of Czech republic, then methods of multiple criteria evaluation.The basic aim of this work was to evaluate the order of business accounts in terms of advantageousness, which are offered by banks on the banking market in the Czech republic. The sectional aim of this work is to get known with the problems of optimal choice of business accounts for a certain group of consumers, which in this case are small businesses. Before choosing a new business account it is always necessary to carefully explore banking market and its offers of business accounts. The important part of this thesis is that the certain client has to consider his preferences before we use quantitative methods. In this diploma thesis has also done a survey about the evaluation of business accounts. Then there are compared results of using methods of multiple criteria evaluation of the survey and model client. In conclusion, the results are described in the order of banking products from the best to the worst business account.
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The prevention of pathological occurence of burnout syndrome (an example of employer)
BLAHOVÁ, Nela
This bachelor thesis deals with the issue of burnout syndrome which is currently a very discuss frequent problem. The rate of working stress will be investigated in selected groups of staff in company COOP družstvo HB. The purpose of my thesis is to compare which group of staff is threatened by burnout syndrome the most. The work is focused on the economical aspects which are reflected particularly in human capital. There will be applied the quantitative research, concretely questionaire research. Based on the results of questionaire research will be applied analytical method of the job evaluation, which is one of the main issues of human resources management. This application will be also recommendation for prevention for the company.
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