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How to prevent the proliferation of Legionella pneumophylis in hot water in the hospital Czech Krumlov
TUREK, Jan
This diploma thesis deals with microbiological quality of hot water in a hospital in Cesky Krumlov as well as hot water piping condition with respect to Legionella. It also deals with arrangements for improving the quality of hot water in this hospital. The subject and target of this thesis are the findings of the mentioned parameters based on own observations, technical documentation and analysis of warm water performed by an accredited laboratory. The hospital in Cesky Krumlov, the construction of which began in 1909, was built in 1911 as the 'Nemocnice Císaře Františka Josefa I.' As the time went by, new buildings were built up. The hospital was renamed to 'Všeobecná veřejná nemocnice' in 1942. In the following years the hospital expanded until the current condition. Water quality and presence of Legionella is a world-wide issue, which we must fight against. The thesis is divided into two parts. First, theoretical part, which includes both general and specific knowledge about Legionella, its health risks, the environment in which it occurs, possibilities of eliminating, suitability of the material for hot water pipes, gained in professional literature and a part of the Czech legislation dealing with public health protection, the law 'No. 258/2004 Coll.' in actual version and quality of drinking water and hot water. In the second, empirical part, I focus on the situation of the hospital in Cesky Krumlov from 2009 till 2015, concerning newly built boiler room and the whole distribution system of hot water regarding Legionella. Particularly I focused on materials used for ecologisation of the gas boiler room and materials used for hot water pipes and actual state of water distribution in individual buildings of this health care facility. I mentioned the analysis of the water samples performed by an accredited laboratory with corresponding technical documentation, which concerns the water distribution system, provided by the medical facility. I used secondary data analysis to evaluate these documents. Regarding the results from 2009 to 2015 the microbiological quality of warm water doesn't meet the limits defined in the Attachment No. 3 of the above mentioned law Order 252/2004 Coll. Limit value for colony forming units of Legionella is set to maximum of 100 CFU. In most of the samples this limit was exceeded by series of units up to hundred thousands of units. The last results in 2015 prove improvement of the unfavorable situation. In that year the analysis was tested three times. First in February, where the CFU units were 'only' in hundreds, with the top value of 550 CFU in the Postacute Care ward. Second analysis was performed in June that same year. Results were acceptable. The highest value was 64 CFU. Last analysis in the hospital was performed in December. Increased concentration of Legionella was again in the Surgery building. There were established of 1400 colony forming units. There were 300 CFU in the Postacute Care ward and the limit value of 100 CFU was in the ward of Internal medicine. The answers to research questions 'How to prevent reproduction of Legionella peumophylis in the water distribution system of the hospital in Cesky Krumlov?' and 'How to ensure a sufficient amount of quality hot water in the medical facility?' are included at the end of the stated recommendations. A risk factor causing the fast reproduction of Legionella is the old water distribution system in most of the buildings and low water temperature of water leaving the boiler, which is 55°C. All mentioned results and evaluations are included in this thesis as well as attached pictures, which show the environment and situation within the Health Care Institutions.
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Nové postupy biomonitoringu cizorodých látek ve vodním prostředí
ČERVENÝ, Daniel
Within the presented work, several approaches of biomonitoring were studied. A well-established method for human health risk assessment was applied to evaluate the quality of fish from open waters in the Czech Republic. As this work is targeted on sport fishermen, who frequently consume their own catches, besides the publication in scientific journal, a brochure with results of this study was distributed via the Czech Fishing Union. Health risks for consumers related to the consumption of wild fish were found strongly species and locality dependent. As there is no risks related with the consumption of carp (Cyprinus carpio) at all investigated sites, frequent consumption of predatory fish should be avoided at some fishing grounds. The use of passive samplers has become more and more attractive in the last two decades. As these devices are able to mimic the biological uptake of chemicals, their potential for replacing fish as bioindicators in routine biomonitoring programmes is evident. In our study, the comparison between analysis of fish tissues and POCIS extracts from selected sampling sites confirms this idea about interchangeability of these indicators in the case of PFASs as target pollutants. Moreover, no metabolic transformation of contaminants present in water take place in passive samplers contrary to living organisms, thus these reflect the actual contamination at the locality more precisely. The use of passive samplers completely fulfils the internationally accepted principles of Replacement, Reduction, Refinement (the three R´s). Another promising approach using YOY fish as bioindicators was evaluated within the present study. Multispecies samples of YOY fish homogenate was found as a better indicator for wide range of pollutants than muscle tissue of adult fish within one species from the same locality. As all inner organs and tissues are presented in analysed samples, more target pollutants in higher concentrations are detected compared to the muscle of adults. Besides the higher sensitivity in pollutants detection and quantification, practical and economic benefits are of concern in the case of the YOY fish approach. The YOY fish approach also particularly fulfil the three R´s strategy, as sampling of this age group of fish has a smaller impact on the environment than the sampling of adults. In case of mercury, that was found as a limiting contaminant for the consumption of fish from open waters in the Czech Republic, a nonlethal method of finclips sampling can be used for the monitoring of this compound in aquatic environments. Besides the monitoring, our optimised method for the estimation of muscle tissue concentrations could be used for human health risk assessment as well. The difference between real muscle concentrations and concentrations estimated using our method differs less than 10% in most of the investigated localities. Benthic organisms are an important part of the food web in aquatic environments, but insufficient information about its contamination is available. In another study included in this thesis, first evidence about bioaccumulation of certain pharmaceuticals in benthic organisms was described. Although, pharmaceuticals are generally considered to be not accumulative in organisms, our study proved that certain pharmaceuticals have bioaccumulation potential. From this finding, it is evident that not only the concentrations of pharmaceutical dissolved in water but also the diet represents an important exposure pathway of contamination by these compounds for organisms (fish) inhabiting aquatic environments.
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Reproductive disorders in dogs
Szabová, Martina ; Rozinek, Jiří (advisor) ; Karlasová, Barbora (referee)
This labour is about reproductive disfunctions of dogs males and females. Prevention and early diagnostics can help to start with medical cure and finaly reinstate animal. Vaginal cytology is cheap, simple, considerate method, which helps define stage of females cycle or diagnose some patological conditions of organism. Bacterial cultivation of vagina or uterus shows natural microflora or pathogenical microorganism, who cause inflammation or sepsis. Sonography helps to find tumors,infections, mineralization or cysts.
The most common tumors are males prostate tumor or females tumor of mammary glands. New studies speculated about global genetic expresion of classification tumors depends on specific examples. Pyometra is one of the most common reproduction disease of females before they reaches ten years. It is an inflammation of uterus with cumulated septic fluid inside and mostly graduaded to ovariohisteroctomy. High concetration of chemokine helps to better diagnose inflammation of organism. Brucella cannis is serious disease, which seriously harms juveniles. In that case we talk about zoonosis which harms humans as well. Common tests who help us to diagnose problems are ELISA, PCR or serology. Canis herpes is expanded around whole world and its prevalency antibodies are very different. For detection of virus is mostly used VNT test, ELISA, PCR or hemaglutination. Abnormal sexual diferentiation is mostly disorder of females reprodaction system, abnormalties of chromosomes and defects on fenotype. Abnormal chromosomes we can categorize as syndrome XXY, syndrom XO, syndrom XXX or real hermaphrodites and chimers. Individuals with defects at gonades have chromosomes XX or XY, but their sexual glands are in contradiction with chromoses. Fenotype problems at individuals mostly have same chromoses as their sexual signs, but their fenotype does not fit to their sexual glands. For example pseudohermaphrodism.
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The Economic Consequences of Organized Crime
Kunt, Lukáš ; Polišenský, Miroslav (advisor) ; Pavel, Pavel (referee)
Summary
This thesis deals with the significance of the threat that organized crime poses to society. The aim is to provide an overview of its present forms and economic impact. In the theoretical part, there is an outline of basic terms associated with organized crime, its traditional and new forms, the most influential organized crime groups and their history regarding the Czech Republic and the EU and institutions that fight them. The empirical part focuses on the issue of illegal drugs. Drugs smuggling and illegal drug trade are defined from the perspective of criminology. Using addictology and police statistics, the features of illegal drug market are described. Comparing various facts, the crackdown on illegal drugs in the Czech Republic and the EU is analysed, and from statistical data - applying mathematical methods - the value of illegal drug market is estimated. Economic impact of chosen forms of organized crime is described by the assessment of their share in GDP and economy in general. In the conclusion of this thesis, some measures that could reduce the social and economic
impact are drawn.
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Psychological and social aspects of unemployment
Kosíková, Monika ; Kebza, Vladimír (advisor) ; Höfrová, Alena (referee)
The final work of my bachelor's degree Psychological and social aspects of unemployment is primarily focused on the Benešov region. There are defined the basic terms in the theoretical part, which are used futher in the other parts of the work. The terms are not defined only in the economic point of view but the emphasis is on psychological aspects of loosing the job. Another way of definition is the social point of view and it describes the impact of unemployment on family and friends of the unemployed. The theoretical part is further given to a calculation of unemployment and to the forms of unemployment. Last but not least there are defined the risk groups of people in the theoretical part, who are put in danger the most by loosing and finding new job the most. The main aim of my work is to compare the evolution of total unemployment in the Benešov region with the other regions of the Central Bohemian Region and to characterize the structure of the unemployed in 2015. The last part is focused on processing the questionnaire in the employment office in Benešov.
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Realization of farm with a focus on poultry
Hromjak, Milan ; Zita, Lukáš (advisor) ; Kovářová, Kateřina (referee)
This thesis deals with the origin of a family farm. By creating a business plan and issues connected with it, its priority is an organic poultry farm, which will produce poultry meat and eggs. Another service beyond the farm production will be creating conditions for a developing branch, which is agro-tourism and other services related to the operation of the farm, so that it leads to the economic development and profit.
The farm will arise by rebuilding of an old house with a large barn and adjacent 10ha of lands inherited from grandparents. There will be built a multi-purpose building called the poultry farm from which the poultry will have access to pasture. The property includes another 1 ha of forest from which the wood will be used as a building material and firewood.
The work is divided into two parts: theoretical and practical. The theoretical part especially focuses on explaining the concepts that are important to understand the business plan.
Then there is the legislation concerning the organic farming, a tangle of laws, public notices and regulations that must be followed in order to meet the conditions for the organic farming. An integral motivational service of the country and the European Union for farmers and not only for those who are environmentally-managing farmers, is support and grants for the years 2014 - 2020. A very important step not only for the starting farmers is a market analysis in the given branch, as well as a SWOT analysis that will show us the positive and negative aspects of the business. The conclusion of this section deals not only with the concept of agro-tourism, but also with its market position as well and possibilities of its use and further development.
In the practical part there is a description of a business plan and individual periods of the project including financing. Finances will flow from own and foreign capital, or the possibility to apply for support through non-refundable grants. The establishment of the farm will have an impact on the labor market, as there will be 5 newly created jobs and the community will fit into the wider public awareness thanks to the activities that the farm will develop for the purpose of product promotion and further development. Furthermore, there are itemized individual items that lead to the final evaluation of the effectiveness of the business plan. By processing the theoretical and practical part of the thesis the requirement of the thesis objective is met - to design a prosperous family farm focused on poultry keeping with a hypothesis if it is currently possible to be competitive while importing poultry meat and eggs from abroad. It can be concluded that the business plan is viable.
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Small sacral buildings in the area Macha region
Sobotková, Lenka ; Zilvar, Josef (advisor) ; Tomáš, Tomáš (referee)
Abstract
The thesis maps and identifies a small religious monuments in the countryside and smaller settlements in the cadastral area municipalities Association Macha, which is located in the southwestern part of the Liberec region and its southern edge, which is Kokorin, extends into the Central Region. These small structures of sacred significance were set up at intersections of roads, trade routes, near Wells, but as expressions of gratitude and remembrance to joyful, significant and tragic event. Each of these small sacred building affects perception of a person's environment, enriching area villages and landscapes, along with accompanying greenery which acted as a refuge on the road, landmark or feature lightning form a common artistic and traditional value. His mission lost partially or completely inappropriate transfer.
The main benefit and purpose of this work is to determine the current state of these monuments in the area, and create a detailed catalog, serving as a source of information for tourism, regional development, such as integration into existing and also into emerging hiking, biking and nature trails, but also as the basis for a partial analysis in the evaluation of the landscape, the creation of the zoning plan, the draft plan during land consolidation or the needs of a particular community.
The catalog is compiled on the basis of professional and regional literature, detailed field survey, including cadastral territory of the municipalities that are part of the voluntary association of municipalities Macha maps and testimonies of some residents is compiled catalog of small sacred buildings. Buildings are photographically documented and described both in terms of the status quo, and in terms of landscape significance here is the location of the building indicating the GPS coordinates and plotting the current actual map of the selected area.
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Change trajectories of wood pastures at the landscape level
Marková, Magdaléna ; Skaloš, Jan (advisor) ; Pavel, Pavel (referee)
The theme of my thesis is analysis and evaluation of development of wood pastures at several locations in the Elbe lowlands from the first half of 19th century up to the present. The area is characterized by its fertility and the large share of arable land.
The data analysis was developed using a geographic information system (GIS). The basic documents were maps and written operate of Stationary cadaster.
The results of the work are map compositions depicting the change trajectories of wood pastures (extinct, continuous and new), graphs show the area of individual trajectories and evaluate the utility of written operate of the Stacionary cadastre.
We have put the obtained information about the natural and social-economic development of the area into context. We got a possible explanation of development, therefore, why the old wooded pastures vanish and new ones arisen elsewhere. Changes of wood pastures were influenced by socio-economic development of the area and probably by distance from a watercourse. The informations obtained from written operate of Stacionary cadaster are useless for this thesis.
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Purpose of the educational trail in a park in Ostrov, "Horažďovice": evaluation, proposals
Lávička, Miroslav ; Skalický, Milan (advisor) ; Jan, Jan (referee)
The thesis deals with educational trails (ET) of various thematic focuses, their purpose and ways of information presentation to visitors through various information panels.
The aim of the thesis was to document an area for a new educational trail in a park in Ostrov in the town Horažďovice. To achieve the goal a sociological research in the town public, employees of educational institutions and municipality was made. Making analyse and evaluation of the place for the educational trail, its madialisation and making an exemplary educational panel was very important.
The thesis suggests several hypothesis: whether town citizens visit educational trails, whether the trail needs an advertisement and if the increased interest has a negative influence in environment of the Ostrov park, whether people accept the educational trail in Ostrov park and if it is convenient for all population groups.
Through the questionaire it was found out that 71% of public visit the trails, 76% of teachers use it as a part of educational process. The public (69%) and the teachers (75%) agree with creating an educational trail in the Ostrov park. Only 11% of public and 19% of teachers know all the ET in the Horažďovice region. The public (78%) and the teachers (89) asked for more information and medialisation of ET. While making a new proposal of the trail and information panel the results of the survey were taken in the account.
Considering the regular upkeeping, facilities, and wheelchair roads, the park was found suitable for people of all ages (families with children, groups of teenagers, seniors, etc.) and disabled people.
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The documentation of the educational trail "Dobrošov" fort (Czech Republic)
Kopecký, Tomáš ; Skalický, Milan (advisor) ; Jan, Jan (referee)
The educational trail Dobrošov fort is situated in the village of the same name. It is an advance specified marked route which leads in the footsteps of the border fortification from 1935 - 1938.
The length of the route is approximately 4 km and visitors are mainly informed about historically interesting places in the surrounding and about the events before World War II. Historical affairs are presented in the form of information panels at individual stops. The panels tell visitors not only about historical military facts of 20th century but also about the building of the fortress fortification Dobrošov.
The educational trail has eight official stops. On the route, visitors can see additional panels with the description of the light fortification and they learn interesting things about the surrounding.
The content of this thesis is a brief characteristic of events in Czechoslovakia before World War II. There is a description of the fortification system of a fort at that time in Europe and Czechoslovakia. Furthermore, this thesis deals with educational trails in general from the viewpoint of their functionality and equipment. This study points out legislation and illustrates new trends of this form of tourism.
The aim of this thesis was to document the educational trail Dobrošov fort, with the focus on historical events of fortification building in Czechoslovakia before World War II. The analysis of the trail and information panels was conducted. A questionnaire survey found out the needs of visitors and highlights the usefulness of this trail. The questionnaire survey had another important role for findings of stated hypotheses. Four scientific hypotheses were formulated. The first one is if the access to more objects increases, its attractiveness and attendance will also increase. The second hypothesis is whether the visitors are satisfied with the infrastructure of the educational trail. Furthermore, the awareness of the educational trail helps to its high attendance, and both citizens and tourists will be happy about a fortification extension which also includes the preservation of decaying objects in the surrounding. The used methods for achieving these aims were the observation and questionnaire survey in the location of the educational trail.
The conclusion shows that the access to more objects will increase the attractiveness and attendance of the trail. However, the visitors are not satisfied with the infrastructure of the trail. The awareness of the educational trail contributes to the high attendance. The hypothesis that local citizens and tourists will be happy about the trail extension which includes the preservation of decaying objects in the surrounding was disproved.
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