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Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch ; Klepárník, Karel
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
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The role of the nurse is to aid in the improvement of the quality of life for children who have been through meningitis
ŠPAČKOVÁ, Jana
The role of the nurse is to aid in the improvement of the quality of life for children who have been through meningitis. In recent years, the issue of quality of life has become of interest in many disciplines and the general public. In health care, the quality of life is known as HRQoL - Health Related Quality of Life - loosely translated as ?the quality of life related to health?. The term of ?diagnostic quality of life in children and adolescents? is primarily used for the area of professional health care and it is an important indicator of disease severity and the outcomes of provided care. The objective of this thesis was to assess the quality of life of children who have been through meningitis, to identify the main areas in which the quality of life of these children is reduced, and to suggest nursing interventions aimed at improvements. The data collection took place in the period November 2009 - June 2010 at the Clinic for Childhood Infectious Diseases, University Hospital Brno. A qualitative research used the technique of studying written documents and, subsequently, non-standardized in-depth interviews with respondents were carried out. Prior the beginning of the qualitative survey, five research questions were established. Question 1: What kind of care and what interventions do children expect from nurses? Question 2: What kind of care and what interventions do parents expect from nurses? Question 3: How to evaluate subjectively the quality of children?s? life after meningitis as compared to the state prior the disease? Question 4: How do parents see the quality of life of their child after meningitis as compared to the state prior the disease? Question 5: In which areas is the quality of children?s life reduced most? Question 6: What nursing interventions can help nurses to improve the children?s lives? All of the questions were answered using a quantitative research method involving a standardized questionnaire named ?HEALTH AND SATISFACTION QUESTIONNAIRE?. This questionnaire is used in the project ?QOLOP? ? Quality of Life Longitudinal Study of Paediatric Oncology Patients. Hypothesis H 1 was defined prior the start of the quantitative research: The quality of life of children who have been through meningitis is lower than in healthy children. This hypothesis was not confirmed. It is clear, from what has been mentioned above, that the objective of this thesis has been met. This project will be useful in practice. The generated ?Algorithm of Initial Care for a Child with Meningitis? and ?Subsequent Care of a Child with Meningitis? should help nurses orientate and identify the basic needs of children with this disease. Moreover, this thesis can be used as a theoretical basis for the education of nursing students.
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Miřenka Čechová
Janečková, Kateřina ; PETIŠKOVÁ, Ladislava (advisor) ; SOPROVÁ, Jana (referee)
Monographic work focuses on artistic creation Miřenka Čechová, one of the
the leading personalities of our contemporary stagecraft. Her personal and
artistic development started when she began studying dance and continued to
mimic and physical theater. Material presents her contribution to
contemporary Czech scene physical theater and refers to her international
success. Work observes her role like co-founder of physical theaters Theatro
Pantomissimo and Spitfire Company and to presents the work of these
physical theaters and reveals the working and teaching methods in the
preparation of copyright performances.
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Mask, Disguise and Role Play in Worldwide Contemporary Self-Portrait
Helfertová, Žofie ; SILVERIO, Robert (advisor) ; DVOŘÁK, Tomáš (referee)
This bachelor thesis deals with the role of mask, disguise and playing with the roles through self-portrait in worldwide contemporary photography. In the first chapters it follows the general development of the self-portrait and a brief history of photography and its forms and tendencies from beginning to the present, it is also focused on the mask in general and role of masks and disguises in the history of photography. Main part of this work is the last chapter, which is devoted to the youngest generation of contemporary photographers. In their self-portraits we can follow tendency which reveals use of mask and disguise for change of their identity. However, they do not create a new identity but takes over, or form the illusion of existing already defined identities. The aim of this thesis is to present the mask as an instrument for role play through a self-portrait.
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Cultural support in Prague's districts
Mokrá, Jolana ; KAŠPAR, David (advisor) ; PROKOP, Petr (referee)
Diploma thesis ”Cultural support in Prague's districts” focuses on a current situation in municipal strategical management and support of culture in all Prague districts. The introduction emphasises the importance of each level of government in the management and support of culture through comparing specific cultural policies. The research is divided into two parts. First one depicts how culture is being understood on the municipal level and presents an overview of existing instruments for support and planning. The second part is focused on a few municipal districts that handle their culture policy with a particular strategy and consider it to be an irreplaceable part of a local development. The second part also examines what tactics and tools these municipal districts use for a realization of their strategic goals. In conclusion, this thesis proves why the role of the local official government is so important in the developement and implementation of culture.
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Survey of continuing care for cardiacs in Czech Republic, with focus on nursing care.
DOLEŽEL, Jakub
Cardiovascular diseases are the leading cause of death in the Czech Republic. An increasing number of its inhabitants become cardiac patients who require a nursing intervention. Nursing in cardiology has an irreplaceable position in the cardiovascular care provision. The health care costs are rising, nursing has been undergoing many changes. Training of cardiology nurses gets changed, as well as the image and role of cardiology nurses in society. Cardiology nurses are much more independent, educated and organized. In cardiovascular facilities nursing care is being standardized and the quality of care is improving. In this thesis, focus is given to nursing in cardiology. In the introduction the issue of cardiovascular diseases is outlined, the major part is devoted to the concept of Czech nursing in cardiology. Individual stages of the system of care for cardiac patients in the Czech Republic and the status of cardiology nurses involved in the system are surveyed. Attention is also paid to the structure of cardiovascular facilities and expert companies in our country and abroad. The thesis has the title: Survey of continuity of care for cardiac patients in the Czech Republic with focus on nursing care. In total five goals, seven research questions and four hypotheses were stated. We surveyed the cardiology nurses´ attitude to lifelong learning, if they are building their own specific image, and if they ensure the continuity of care. Our objective was also to identify the role of nurses in education of cardiac patients and the level of cardiac patients´ knowledge. The research section is elaborated on the basis of a qualitative and quantitative investigation. The qualitative part was carried out by semi-structured interviews with cardiology nurses working in comprehensive cardiovascular centers. The quantitative part of the survey was conducted with patients of comprehensive cardiovascular centers. Seventy domestic and foreign sources of literature devoted not only to the issueof cardiovascular diseases were quoted. The research results are supposed to serve as a basis for coordinated efforts of all participating nurses and institutions to build a well-functioning and technically and economically efficient system of nursing care for cardiac patients in CR.
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Evaluation of the effectiveness of teaching vocational subjects depending on physical activity before schooling.
Janošíková, Martina ; Hanušová, Marie (advisor)
The period of transition to secondary school brings substantial changes in the requirements for study obligations, autonomy, communication, expression, or separated from their families in the form of staying at the dormitory. Students enrolled in sport centers, in addition to these changes, still struggling with the responsibilities associated with sport. Because they are students first through fourth grades of secondary schools and secondary vocational schools, an age when image self-I am, what I believe in, arises in interactions with the outside world and their own experience, plays a very important role to influence the environment, educators, teachers and trainers, with which students spend most of their time in practice. This study looks at students comprehensively in terms of personal development as a multifactorial biopsychosocial unit, in terms of cognitive and motivational processes, states and properties from the perspective of social psychology dealing with the interactions, relationships and communication, psychology of individuals within the social group and its importance for the development of the individual . It monitors conditions and educational outcomes in terms of educational psychology, dealing with forming properties, opinions, attitudes and value orientation from the perspective of educational psychology.
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The occurrence of domestic and wild forms of the camels in the old world and their economic use
Burgerová, Michaela ; Gardiánová, Ivana (advisor) ; Jan, Jan (referee)
Genus camel (Camelus) includes two species, the Dromedary camel (Camelus dromedarius) and Bactrian camel (Camelus bactrianus). Camels have a wide range of utilization. People in Arabia countries but also in India and Egypt are using camels as a milk and meat animal, or even as a transport and companion animals and riding animals too. The aim of this thesis was to find sources about camel keeping and using and his role of farm animal in different cultures and countries. And about conditions and requirements in captivity. The first part of thesis contains information about phylogeny and origin of the camel family, taxonomy of camels, short information about the history of domestication and using of camels. There are also described the two species in terms of species biology etc. plus a brief mention of camel hybrids and camel breeds. The second part focuses on the use of camels. The preffered and most common use is in the production of milk, other dairy products from camel milk, fermentation of milk. Another use is for meat performance, meat composition, describes products. His utilization i also useful for other parts such as hair wool and mane, they come mainly from bactrian camel (Camelus bactrianus). The last is working utilization of camels, carrying baggage. Then hobby use. The thesis describes the conditions of keeping camels in captivity, keeping camels in Somalia, requirements for the supply of water and food, housing conditions in the Czech Republic, it is particularly widespread keeping camels in zoos. In Czech Republic they are small private camel breeders. For comparison they are conditions for keeping camels in captivity in Australia.
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Fish passes and their role in landscape and ecosystem management
Míka, Martin ; Horký, Pavel (advisor) ; Kuříková, Pavlína (referee)
Fishways are devices intended to restore fish migration through the transverse obstacles in fragmented flows. Basic types of fish passages can be divided into technical and close to nature. Technical as cellular or crevice between the close to nature can include, for example, a bypass channel or boulder ramps. Close to nature fishways are suitable not only for Migration wider range of species and the size of fish, but they are preferable as landscape elements in urban landscapes. Can be recommended to be close to nature when crossing streams fragmented preferred, although not necessarily their use, for example, due to space constraints at a particular location possible.
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