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Design of the electron microscope
Švajdová, Anna ; Surman, Martin (referee) ; Škaroupka, David (advisor)
The theme of this diploma thesis is the design of a scanning electron microscope with a focused ion beam. Specifically, the thesis is focused on the design of the microscope covers and the adjacent workplace of the operator for Tescan Orsay Holding a.s.. Design is solved as the first proposal aimed at future innovation of the design of the entire product line.
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Nozzle separation valve design for rasterization electron microscope
Hoffman, Petr ; Navrátil, Václav (referee) ; Foltýnová, Dana (advisor)
The aim of the thesis is a design and subsequent construction of a vacuum separation valve for a scanning electron microscope. The introduction describes the functions of an isolating valve. The function of the scanning electron microscope is described in the overview of the current state of knowledge. The following chapters focus on conceptual designs of the valve, their descriptions, advantages and disadvantages. Based on the comparison, the most suitable solution was selected. A force analysis of the selected variant is also performed and described. The end of the thesis contains the whole structure design and processed drawing documentation.
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Optimization of UHV SEM for nanostructure study in wide temperature range
Axman, Tomáš ; Zigo,, Juraj (referee) ; Bábor, Petr (advisor)
This diploma thesis deals with the optimization of ultra-high vacuum scanning electron microscope - UHV SEM, which is developed within the Amispec project in cooperation with BUT, Institute of Scientific Instruments of the Czech Academy of Science and Tescan Brno, s.r.o. The theoretical part deals with the description of the actual state of the developed equipment and the research of competing systems. The next part describes the optimization of the sample holder and the pallet receptor for studying nanostructures over a wide range of temperatures. Part of the optimization is the sapphire thermal diode development and experimental verification of the functionality of the designed components. This is followed by the verification of the functionality of the whole system for the transport of samples to the UHV area, deposition with effusion cell and in-situ observations.
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Automation of Exoscopic Analysis Using Image Processing of Sedimentary Grains Acquired by Electron Microscope
Křupka, Aleš ; Křížek,, Marek (referee) ; Baroňák, Ivan (referee) ; Říha, Kamil (advisor)
This thesis deals with image analysis methods which can be exploited in exoscopic analysis of sedimentary grains, specifically for the purpose of distinguishing between geomorphologic geneses which influenced a form of sedimentary grains. The images of sedimentary grains were acquired by a scanning electron microscope. The main contribution is the proposal of multiple methods that can significantly automate the exoscopic analysis. These methods cover the automatic segmentation of grains in image, the automatic analysis of roundness of 2D grain projection and the classification of geomorphologic geneses according to the grain surface structure. In the section concerning the automatic segmentation, a segmentation method enabling an easy subsequent manual result correction was proposed. This method is based on the split-and-merge approach. The individual steps the procedure were designed to exploit specific properties of sedimentary grain images in order to obtain the best segmentation results. In the section concerning the automatic roundness analysis of 2D projection of sedimentary grains, an influence of pixel resolution on a result roundness value was evaluated. Further, a minimal number of grains, which is necessary to analyze in order to reliably compare a pair of geomorphological geneses, was investigated. For the determination of this number, a method was proposed and experimentally verified. In the section of automatic analysis of sedimentary grain surface structure, a method for classification of geomorphologic geneses was proposed. The method utilizes low-level texture features which describes individual images of sedimentary grains. A model of geomorphological genesis is constituted of a set of histograms representing occurrences of different configurations of low-level texture features. The methods proposed in the thesis were tested and evaluated based on a database, which consists of sedimentary grain samples from 4 different geomorphological geneses (eolic, glacial, slope and volcanic).
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Characterization of structures fabricated by selective wet etching of silicon
Metelka, Ondřej ; Mikulík, Petr (referee) ; Šamořil, Tomáš (advisor)
The task of master’s thesis was to perform optimalization process for preparing metal etching mask by electron beam litography and subsequent selective wet ething of silicon with crystalographic orientation (100). Further characterization of etched surface and fabricated structures was performed. In particular, attention was given to the morphology demonstrated by scanning electron microscopy and study changes of the optical properties of gold plasmonic antennas due to their undercut.
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Detection of signal electrons in ESEM
Šváb, Martin ; Špinka, Jiří (referee) ; Jirák, Josef (advisor)
This bachelor's thesis deals with problems of environmental scanning electron microscopy and with detection of signal electrons at higher pressure in working chamber. In this thesis I will focus on comparison of detected signal level achieved under different working conditions.
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Study on biotechnological potential of thermophilic gram-positive bacterium Brevibacillus sp. Bz
Filimonova, Anastasiia ; Obruča, Stanislav (referee) ; Pernicová, Iva (advisor)
Předmětem předložené diplomové práce je studium biotechnologického potenciálu termofilní grampozitivní bakterie Brevibacillus borstelensis BZ. Teoretická část obsahuje obecnou charakterizaci termofilních organismů a jejich termozymů. Popisuje také adaptivní molekulární mechanismy, které zajišťují termostabilitu těchto proteinů. Závěr teoretické části je věnován biodegradaci odpadních substrátů a polymerů na bázi přírodních a fosilních zdrojů. První část experimentální práce se zabývá produkcí hydrolytických enzymů na různých původních zdrojích a odpadních substrátech. Díky intenzivní enzymatické produkci na původních zdrojích je Brevibacillus borstelensis BZ považován za velmi slibného producenta termostabilních enzymů, konkrétně xylanáz a celuláz. Testovaný bakteriální kmen BZ byl schopen produkce termostabilních enzymů i na odpadních substrátech. Na vybraných substrátech kmen BZ přednostně produkoval xylanázy. Díky tomu byla xylanázám věnována zvláštní pozornost, kdy bylo testováno teplotní a pH optimum. Závěrem experimentální práce byla testována schopnost bakterie Brevibacillus borstelensis BZ degradovat vybrané polymery na bázi přírodních a fosilních zdrojů. Kmen BZ poskytl nový pohled na biologický rozklad polyethylentereftalátu (PET), amorfní frakce kyseliny polymléčné (PLA), semikrystalické PLA a polyhydroxyalkanoátů (PHA). Pozorováním povrchu PET fólie skenovacím elektronovým mikroskopem (SEM) se potvrdilo zdrsnění materiálu, přítomnost rýh a naprosté pronikání bakterie skrz fólii. Pokud jde o polymery na bázi přírodních zdrojů, PHA granule byly zcela degradovány. Studiem morfologie povrchu obou zmíněných PLA bylo prokázáno jasné zhoršení jejich struktury přítomností jam a trhlin na povrchu polymerů.
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Temperature drift compensation for nanostructure analysis
Hakira, Stanli ; Páleníček, Michal (referee) ; Bábor, Petr (advisor)
An ultra high vacuum apparatus for nanostructure experiments is being developed by the Tescan company in cooperation with the Institute of Physical Engineering of the Faculty of Mechanical Engineering. The apparatus is designed for preparation, modification, and analysis of nano-scale structures. A sample holder which allows heating and cooling has been developed for the apparatus. A scanning electron microscope is attached to the chamber to provide analytical and manufacturing capabilities. During experiments with heating enabled, the sample moves relative to the SEM column, causing drift of the image. This bachelor thesis proposes a solution to the problem of temperature drift by the means of motion tracking based on image registration using Fourier transform. An application complementary to the SEM control software which implements the algorithm was designed and tested at the instrument.
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Influence of working conditions on the signal level detected by BSE detector
Bednář, Eduard ; Zimáková, Jana (referee) ; Čudek, Pavel (advisor)
This thesis deals with the evaluation of the quality of the signal level of backscattered electrons detected by the scintillation backscattered electron detector depending on the working conditions in low vacuum scanning electron microscope. The theoretical part describes the issue of environmental scanning electron microscopy, the principle of generation the signal and detection of backscattered electrons. The experimental part of the thesis is to measure the properties of the scintillation detector of backscattered electrons. A series of experiments is evaluated the influence of working conditions on the stability and function of the detector of backscattered electron.
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Experimental evaluation of aluminothermic welds
Zeman, L. ; Valach, J. ; Zlámal, P. ; Krčmářová, N. ; Koudelková, Veronika ; Zeman, J.
The article presents a study of the mechanical processes occurring during the aluminother-mic reaction using experimental methods (strain gauges, digital image correlation, thermography,scanning electron microscopy, profilometry). The aluminothermic reaction is a highly efficient weldingmethod due to its exothermic behaviour, however, it places considerable demands not only on thewelding technique, but also on the capabilities of the experimental methods used - these limitationsare also discussed in the article. The aluminothermic reaction is associated with the formation of alocalised heat source with a time evolution dictated by the technological procedure, which manifestsitself in heat propagation to the surrounding weld material. The unequal evolution of the temperaturefield is the fundamental cause of the appearance of the heat affected zone or local deformations orsurface curvature, which was the focus of the experimental methods deployed above and the results ofwhich are shown in the article.
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