|
|
Study on biotechnological potential of thermophilic gram-positive bacterium Brevibacillus sp. Bz
Filimonova, Anastasiia ; Obruča, Stanislav (oponent) ; Pernicová, Iva (vedoucí práce)
The subject of the Master’s theses is to study the biotechnological potential of the thermophilic Gram-positive bacterium Brevibacillus borstelensis BZ. The theoretical part contains a general characterization of thermophilic organisms and their derived thermozymes, and describes their adaptive molecular mechanisms in protein thermostability. The final part of the theoretical part focuses on biodegradation of waste substrates, biobased and fossil-based polymers. The first experimental part deals with the production of hydrolytic enzymes on various original sources and waste substrates. Brevibacillus borstelensis BZ is reputed to be a highly promising source of thermostable enzymes, namely xylanases and cellulases, due to its high enzymatic production on original sources. Secondly, the strain BZ was capable of producing a thermostable hydrolytic enzyme on waste substrates. On these substrates, a selected strain preferably produces xylanases. As a consequence, thermostable xylanase has gained a special attention, and, subsequently, it was assayed for the identification of its optimal pH and temperature. The final part of the experimental work discovers the biodegradation capacity of Brevibacillus borstelensis BZ toward selected biobased and fossil-based polymers. The strain BZ provided new insight into biodegradation of polyethylene terephthalate (PET), amorphous fraction of polylactic acid (PLA), semicrystalline PLA, and polyhydroxyalkanoates (PHA). Scanning electron microscope (SEM) observations confirmed an occurrence of roughness surface, the presence of grooves, and an utter penetration of the bacterium through the PET film. Regarding biobased polymers, PHA granules treated with the strain BZ were completely degraded. By studying the surface morphology of both types of PLA, the results indicated a clear deterioration of the structure and the presence of pits and cracks on the surfaces.
|
|
|
Study on biotechnological potential of thermophilic gram-positive bacterium Brevibacillus sp. Bz
Filimonova, Anastasiia ; Obruča, Stanislav (oponent) ; Pernicová, Iva (vedoucí práce)
The subject of the Master’s theses is to study the biotechnological potential of the thermophilic Gram-positive bacterium Brevibacillus borstelensis BZ. The theoretical part contains a general characterization of thermophilic organisms and their derived thermozymes, and describes their adaptive molecular mechanisms in protein thermostability. The final part of the theoretical part focuses on biodegradation of waste substrates, biobased and fossil-based polymers. The first experimental part deals with the production of hydrolytic enzymes on various original sources and waste substrates. Brevibacillus borstelensis BZ is reputed to be a highly promising source of thermostable enzymes, namely xylanases and cellulases, due to its high enzymatic production on original sources. Secondly, the strain BZ was capable of producing a thermostable hydrolytic enzyme on waste substrates. On these substrates, a selected strain preferably produces xylanases. As a consequence, thermostable xylanase has gained a special attention, and, subsequently, it was assayed for the identification of its optimal pH and temperature. The final part of the experimental work discovers the biodegradation capacity of Brevibacillus borstelensis BZ toward selected biobased and fossil-based polymers. The strain BZ provided new insight into biodegradation of polyethylene terephthalate (PET), amorphous fraction of polylactic acid (PLA), semicrystalline PLA, and polyhydroxyalkanoates (PHA). Scanning electron microscope (SEM) observations confirmed an occurrence of roughness surface, the presence of grooves, and an utter penetration of the bacterium through the PET film. Regarding biobased polymers, PHA granules treated with the strain BZ were completely degraded. By studying the surface morphology of both types of PLA, the results indicated a clear deterioration of the structure and the presence of pits and cracks on the surfaces.
|
host ::
RSS.