Národní úložiště šedé literatury Nalezeno 5,475 záznamů.  začátekpředchozí21 - 30dalšíkonec  přejít na záznam: Hledání trvalo 0.33 vteřin. 

Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
Čapka, Lukáš ; Mikuška, Pavel ; Večeřa, Zbyněk
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. The system was applied to the determination of\nDCAs in urban aerosols in Brno. The method was verified using a laboratory generated\nstandard aerosol of DCAs. A sampling of aerosol particles on parallel filter was used\nas a reference method.

Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
Lačná, J. ; Foret, František ; Kubáň, Petr
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new CE-LIF system. Then the derivatization process\nof MDA by TBA was optimized. The ability to use this method for the detection of\nMDA in a biological samples, such as EBC and plasma, was also demonstrated. For the\nfirst time it was possible to detect MDA in EBC samples by CE. Other biomarkers are\nnow being studied.

Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch ; Klepárník, Karel
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.

Dynamické vlastnosti rotoru kmitajícího v tekutině
Chlud, Michal ; Pochylý, František (oponent) ; Malenovský, Eduard (vedoucí práce)
Tato práce se zabývá dynamickými vlastnostmi rotoru vírové turbíny. Primárně je studován vliv snížení vlastních frekvencí rotoru v důsledku interakce s vodním prostředím, a to pro různé úrovně ponoření rotoru do kapaliny. Následuje zjištění vlastních frekvencí rotoru vírové turbíny při provozních otáčkách. Problém je řešen výpočtovým modelováním v systému ANSYS, kdy je tekutina modelována pomocí akustických prvků. Dosažené výsledky jsou porovnány s experimentem.


Detekce síťových anomálií na základě NetFlow dat
Czudek, Marek ; Bartoš, Václav (oponent) ; Kořenek, Jan (vedoucí práce)
Tato práce se zabývá využitím NetFlow dat v systémech pro detekci narušení nebo anomálií v síťovém provozu.Práce zahrnuje popis způsobů, které se využívají pro sběr dat ze síťě. Rozsáhleji je zde popsán protokol NetFlow. Následně se práce zaměřuje na analýzu a popis různých metod, které se využívají pro detekci anomálií v síťovém provozu, se zhodnocením jejich výhod a nevýhod. Na základě analýzy těchto metod je v další části práce vybrána konkrétní metoda. Následně je provedena analýza datové sady s využitím vybrané metody. Na základě výsledků je navržen algoritmus pro detekci anomálií v reálném čase. Tato konkrétní metoda byla vybrána na základě toho, že je schopna detekovat anomálie v neoznačeném síťovém provozu. V poslední části práce je algoritmus implementován a jsou provedeny experimenty s výslednou aplikací nad reálnými NetFlow daty.

Recovering of Company's Website
Lang, Michal ; Lohr, Václav (vedoucí práce) ; Martin, Martin (oponent)
Summary Small business operating in villages usually face the problem of lack of customers, especially if they offer luxury products. The budget of such companies is often limited and therefore they cannot afford an expensive advertising. The company website is one of the few solutions that can get customers from outside the rural areas and thus create new business opportunities and increase revenues of such companies. The main goal of the thesis is to attract customers to a company website and thus help small businesses seeking customers in their difficult situation. The theoretical part starts with the basics. It describes what systems are suitable for different kind of web pages and the main part is focused on factors that influence an overall website performance. The second part shows how to achieve the stated goal on an example of a small joinery company Atyp truhlářství Lang. The company is present on the Internet since April, 2009. The work with a real website allows to compare results before and after the changes implementation. The practical part starts with an analysis of the original website. It determines strengths and weaknesses of the web and it provides suggestions for improvement. After this, the academic work deals with the implementation of suitable factors. The final chapter evaluates the original and new version of the site and represents how the made changes are reflected in the site traffic. Keywords Website design, SEO, on-page factors, off-page factors, CMS, Content Management Systems, increase site traffic Objectives and Methodology The main objective of diploma thesis is to increase the website traffic, its attractiveness for customers and thus help small companies located in rural areas to get new clients. The first part helps to achieve the stated goal by dealing with aspects influencing the overall web page performance and also provide basic knowledge related to the website design. Consequently, the sub-objective of this part is an identification of factors that significantly affect the site traffic. The second part aims to apply the gained knowledge on an example web page of small company Atyp truhlářství which faces a problem of a lack of customers. It starts with the analysis of the original website that shows its strangeness and weaknesses. This will provide an information about possible improvements. The part of authors own work aims to implement suitable factors from the theoretical part into the website design. The objective of the final part is to compare the changes made with the original design and determine if the new site solution has really improved the number of visits and its attractiveness for customer. 2 The essential information for the masters thesis elaboration were obtained from an analysis of printed sources as professional books, journals, annual reports and online resources as well. Statistical data reflecting the original website traffic were extracted from MySQL database. The new data about number of visitors were already get in friendlier form of Google Analytics outputs. In addition to that, author also discussed his academic work with website experts and used his own knowledge acquired during the university studies of information technology. Results and Conclusion The website overall performance is greatly influenced by the system on which it operates. For this reason, the literature review provides an overview about Content Management Systems and Static Site Generators. There are explained differences between these two approaches to a website design and are given recommendations about what kind of pages are suitable for each approach. The main part identifies 30 on-page and off-page factors that have a positive or negative impact on the final website traffic. The practical part consists an analysis of the examined site. It discovered the following weaknesses. The positioning is very bad in Search Engine Result Page and it is almost impossible to find the web without an exact knowledge of the company name or its location. Content can be considered as extremely poor with overall 279 words only. There were also two technical issues. The first caused a traffic data loss for the last five years due to exceeding 50MB of MySQL memory limit. The second was related to plugins deployment that used up the whole PHP memory limit. It resulted in WordPress strong unstability. The website speed test was disastrous. The small page size of 762,52kB was loaded for 4,38 seconds. An acceptable result would be less than 2 seconds. Concerning security, the site did not use secured protocol and the next risk could pose deployed plugins that are frequent targets of hacker attacks. The used template was not mobile-friendly and therefore it was difficult to browse the pages on mobile device. In addition to that the graphical appearance had a low attracting power, it was not able to adequately impress the customers. The site was visited by an average of 25 people a day in 2011. Furthermore, only 63% of visitors come from the Czech Republic. Unfortunately, more actual data could not be obtained because of the data loss. After considering of all mentioned issues above was proposed a complete website recovery with an implementation of suitable factors from literature review. The examined website is extremely static without a dynamic content and the frequency of updates is relatively low. The web pages are managed by a technical contributor and it is the reason why graphical user interface of CMS is not required. After evaluation of all previous technical, speed and security issues was selected a static site generator for the website design. 3 The important chapter is represented by implementation of suitable factors that should as a complex lead to an increase of a site traffic. First important factor is a content. The original word count of 279 words was improved to 1809 words which is 7 times more than in the past. Before writing the articles, an analysis of relevant terms were made. Those terms are included across the web content. The new articles are meaningful and they provide product details and customer reviews. Moreover, the content is structured, there are 9 unordered lists included and main titles are differentiated by importance. Each page has internal links to other subpages. The text clarity and an inclusion of relevant terms are equal to a better rank of a page. The number of images has decreased from 112 original images to selected 42 images on the new website. This is because of a very bad image quality of most old pictures. The high quality images can cause customer interest for the product, higher time on site and lower bounce rate. Meta descriptions were implemented individually for each web page with regard to the page content. The new website was accepted by a certification authority (CA) and the unsecured Hypertext Transfer Protocol was replaced by Secured Hypertext Transfer Protocol (HTTPS). It means the site is trustable and secure for browsing. There are no plugins used and therefore an attack risk is much lower. The template was tested on 17 screen resolutions. All tests were successful and the site is fully responsive. Almost 33% of customers use some mobile device to browse a web. The current web eliminates the loss of visitors coming from mobile devices. Furthermore, Google officially announced that secured and responsive websites get priority. The site speed test had two rounds. The first test can be considered as very good. The page load time was 842ms and overall page size was 1.1.MB. Nevertheless, after the compression of large files the site became even faster. The page size decreased by 30% to 771,6kB and the final load time speed up by 31% to 580ms. Currently, the website is faster than 96% of all tested domains which is a perfect score. The adjusted site speed is 7,5 times faster than the original speed. No flash, advertisement or other disruptive elements are available on the web. The web page is newly available on Google+, Pinterest and Twitter. These factors are able to boost direct page traffic. The design is clean and simple just to avoid leaving customers confused. The readability is also very good. Combination of black/blue text on the white background or white text on the black/blue background doesnt cause any readability issues. Social buttons and a quick company contact is present on each page in header and footer section. Another change was made in traffic measurement. The website started to use Google Analytics for tracking visitors. The actual traffic data are more accurate and the possibility of data loss is reduced. The main goal of diploma thesis was to improve the website traffic. The web page was measured from 01.12.2015 to 29.02.2016. The average site traffic was 23,0 visits a day in December 2015. This number reflects the original site traffic which was around 25 visits a day with a decrease of 8%. The biggest change in traffic development was found between 08.01.2016 to 13.01.2016 when the average site traffic was 77,2 visits a day. Those changes are explained by an influence of social media. Author has written a post about Atyp truhlářství on Facebook on 8th January 2016. The post was shared by 8 people and it attracted many people to visit the website. 4 From January 14th to the end of the month, the average daily traffic was 32,3 visits. The last month was noted slightly higher traffic of 32,6 visits a day. The comparison of data from December 2015 and from February 2016 shows a visible increase in number of visits in February 2016 where the traffic was higher by 41,7%. There is a positive increasing trend in a number of visits since the beginning of December 2015. Moreover, majority of visitors 81,3% come from the Czech Republic and it is a better result than by the original web pages with 62% only. The recovery of a small joinery website atyptruhlarstvi.cz was successful. It improved the website traffic and many other aspects as content, speed, security, responsiveness, social media etc. The web design itself and implemented factors can be considered as crucial elements that influence the final number of visits. For this reason, the made changes on a web page can really help small business in rural areas to get new customers. There were also identified factors that affect an overall website performance with an emphasis on a positive site traffic development. An overview about different website design approaches is provided as well. References SEARCHMETRICS. Search Ranking Factors and Rank Correlations: Understand how the deck is stacked [online]. San Mateo, 2015. Accessible from: http://www.searchmetrics.com/wpcontent/uploads/Ranking-Factors-2015-Whitepaper-US.pdf COSTELLO, Vic, Susan A YOUNGBLOOD a Norman YOUNGBLOOD.Multimedia foundations: core concepts for digital design. New York: Focal press, Taylor & Francis group, 2013. ISBN 978-0-240-81394-3. FIELDING, Jonathan. Beginning responsive web design with HTML5 and CSS3 / Jonathan Fielding. New York, New York: Apress, 2014. Experts voice in Web development. ISBN 9781430266945. MITCHELL, Melanie. Standing out with Seo: Expert advice from Melanie Mitchell (collection). United States: FT Press, 2013. ISBN 978-0-13-344320-2. BROWN, Bruce C. How to build your own web site with little or no money: the complete guide for business and personal use. Ocala, Fla.: Atlantic Pub. Group, c2010. ISBN 1601383045. ELMANSY, Rafiq. Search engine optimization. Indianapolis, IN: John Wiley & Sons, Inc., 2013. Teach yourself visually. ISBN 1118470664.


Antiplagiátorské nástroje pro naše repozitáře
Mach, Jan
Prezentace je zaměřena na test a komparační analýzu systémů na odhalování duplicit (tzv. antiplagiátorských systémů) vhodných pro repozitáře vysokoškolských kvalifikačních prací v ČR. V příspěvku bude popsán proces testování a budou prezentovány výsledky, které mohou pomoci při rozhodování o vhodném antiplagiátorském nástroji pro kontrolu kvalifikačních prací i dalších typů šedé literatury.
Plný text: idr-692_3 - Stáhnout plný textPDF
Prezentace: idr-692_1 - Stáhnout plný textPDF; idr-692_2 - Stáhnout plný textPDF
Videozáznam: idr-692_4 - Stáhnout plný textMP4

Návrh technologie výroby zátky z plastu
Kočí, Ivan ; Žák, Ladislav (oponent) ; Kandus, Bohumil (vedoucí práce)
Diplomová práce se zabývá návrhem výroby zátky z plastu technologií vstřikování do forem. V práci je proveden návrh technologického postupu včetně potřebných výpočtů a mimo to je práce zaměřená na konstrukci vstřikovací formy. Pro řešení byl použit 3D CAD systém SolidWorks a normálie Hasco, Meusburger, Synventive. Na závěr je práce podložena technicko-ekonomickým zhodnocením.