National Repository of Grey Literature 44 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Copper metabolism in Trypanosoma brucei
Sandner, Bruno ; Mach, Jan (advisor) ; Štáfková, Jitka (referee)
Copper serves as a cofactor for many important enzymes due to its redox properties. However, the presence of free copper ions in the cell can lead to undesired effects such as protein aggregation and loss of iron-sulfur clusters, ultimately resulting in cell death. Therefore, the intracellular concentration of copper in parasitic organisms is strictly controlled. Copper metabolism in parasitic organisms is not well understood, hence this work focuses on Trypanosoma brucei, the causative agent of sleeping sickness and the disease in cattle called nagana. The aim of this thesis was to investigate how copper is transported in the cell and to test its toxicity in T. brucei. Understanding copper transport in the cell could lead to the development of drugs disrupting copper homeostasis and exploit of its toxic properties. In this diploma thesis, the function of the P1B-type ATPase of T. brucei in copper transport was described based on functional complementation. Successfully, the expression of the P1B-type ATPase was reduced using RNAinterference in T. brucei. The decreased expression of this transporter led to increased sensitivity to copper, indicating its role in copper detoxification. Furthermore, we demonstrated the higher resistance to copper and its increased requirement in the metabolism of the...
Project - Organization of Dance Marathon
Fikejzová, Kristýna ; Mach, Jan (referee) ; Kysel, Jiří (advisor)
This bachelor thesis deals with organization of dance marathon. In a theoretical part some terms are characterized, such as a manager, project management and sport marketing. The readers are also given information about dance features and characteristics. I used the survey and SWOT analysis to analyze the present state. Furthermore I deal with preparation and organization of dance marathon including the proposals for its innovation.
Possibility of hazardous environment monitoring
Mach, Jan ; Musil, Martin (referee) ; Blecha, Petr (advisor)
This bachelor’s thesis is about mobile robotics and usage it in electricity industry. First part solve ability of taking the fences and provide basic facts about mobile robotics, kind of chassis and purpose of usage to reader. Second part includes concepts of truck and position device with monitoring system. This concepts solve submission with reference to utility of monitoring device, mobile robot and price. Output of this work is constructive solving of mobile monitoring robot.
Physical Phenomenon as a Creative Method in Architecture
Buryová, Tereza ; Mach, Jan (referee) ; Kristek,, Jan (advisor)
Cellular_ polar research station Antarctica is the driest, the busiest and most extensive place on Earth, half-year-shrouded. People still live here. They set up the research stations in which they live and work. In these extreme climatic conditions, the station originates and disappears due to natural behaving. Stations provide researchers with safety and space for research, including comfort. They are built in different ways and provide different comforts, according to technological and transport options.
Design optimisation and strenght checking of Water tiltable devices
Mach, Jan ; Holub, Michal (referee) ; Knoflíček, Radek (advisor)
This master’s thesis is about possibility of fruit and vegetable process from agricultural raw material to food. The first part of thesis include information about machinery, devices and process line and describe how this devices work. The second part solve water tiltable device, this part explain what water tiltable device do, how work and describe structure of each subassembly. Next step solve the device by kinematics aspect and aspect of load analysis. Calculation is included in annex one. Results of annex one are used for FEM (finite element method) simulation of frame. Output of FEM simulation can be used for finding safety factor of displacement and strain, output data are used for optimization of device.
Treatment and drug development against free-living pathogenic amoebae
Jankovcová, Klára-Marie ; Mach, Jan (advisor) ; Vinopalová, Martina (referee)
Free-living pathogenic amoebae are cosmopolitan unicellular eukaryotic organisms, which can cause several serious infections in humans and animals. They are causative agents of amoebic encephalitis, which are very rare and acute diseases of the central nervous system, that almost always lead to the death of the patient. Even though the importance of these pathogens has been increasing in recent years, the development of treatment procedures and drugs against them is very ineffective and unsatisfactory. Treatment and drug development is complicated mainly because of the complex biological structure of pathogens and limited knowledge of metabolic pathways and the biochemical reactions that are occurring in them, which are crucial for the development of effective drugs. In this bachelor thesis I focus on the pathogenic species Naegleria fowleri, Balamuthia mandrillaris and the pathogenic genus Acanthamoeba, which is the causative agent of encephalitis and acanthamoeba keratitis. In the first part of this bachelor thesis, I generally characterize the different representatives of free- living pathogenic amoebae and describe the clinical picture of individual diseases. In the second part I address the issue of treatment. Firstly, I describe current therapeutic options and issues of the treatment of...
Effect of lipophilic cation on the toxicity of phosphonium salts in parasitic protists
Čadková, Aneta ; Mach, Jan (advisor) ; Trnka, Jan (referee)
The mitochondrion is an essential organelle responsible for energy metabolism and maintaining cellular homeostasis. Disruption of their function can have serious consequences for the entire cell and organism. Given their key role in the organism, they become an attractive target for drugs. In this thesis, we examine the impact and significance of phosphonium salts as potential mitochondrial-targeted therapeutics. The investigated compounds have modified anchors based on the commonly used triphenylphosphonium cation (TPP+ ). These substances have the ability to permeate the phospholipid bilayer and accumulate on the inner mitochondrial membrane. Our research focuses on selected model organisms of the class Kinetoplastida and opportunistic pathogenic fungi. These pathogenic organisms are responsible for severe infectious diseases in humans. Our aim is to compare the effectiveness of phosphonium salts with different anchor modifications against these organisms and attempt to elucidate their mechanism of action. We have found that the modified salts are capable of inhibiting the growth of parasitic protists at nM concentrations, affecting mitochondrial respiration, membrane potential, and mitochondrial integrity. However, there is no increase in the level of oxygen radicals. We are assuming that the...
Treatment options for untreatable amoeba Naegleria fowleri
Veselý, Martin ; Mach, Jan (advisor) ; Arbon, Dominik (referee)
Naegleria fowleri is a free-living protist which can penetrate the body of a previously healthy person and cause meningoencephalitis. The rapid course, disproportionately strong immune response and the lack of effective drugs are the reasons why this disease almost always ends fatally. Due to the relative rarity of this disease, it is not lucrative for pharmaceutical companies to develop a completely new drug. The only way to search for effective drugs is to test those that have already been approved for another purpose. However, clinical tests are not possible due to the low morbidity and rapid course of the disease, and research in this area is thus limited to animal models of the disease and evaluation of the effect of drugs against the pathogen in vitro. Currently used medication includes drugs that are highly toxic or poorly penetrate the site of inflammation. In the future, more effective chemotherapy against N. fowleri and more gentle towards patient could be used. This work summarizes knowledge about pathogenic amoeba, currently used drugs and presents new experimentally tested therapeutics.
Selective screening of antiparasitic substances
Wernerová, Klára ; Mach, Jan (advisor) ; Kovářová, Julie (referee)
The mitochondrion is the key organelle of the cell. It is involved in energy metabolism, the Krebs cycle, the respiratory chain, and the formation of Fe-S clusters, which form the structure of many proteins. Therefore, disruption of the mitochondria is usually fatal to the cell, especially if it contains only one mitochondrion, as is the case in Trypanosoma brucei brucei. The T. b. brucei parasite has become the primary model organism for our study because its basic biochemical processes are already well described, including mitochondrial metabolism, and it is also easy to cultivate in the laboratory. Other model organisms we used in this study were Leishmania mexicana, Cryptococcus neoformans and Candida albicans. All these organisms are causative agents of serious diseases, and although there are drugs against them, new drugs need to be developed due to the frequent emergence of resistances. Mitochondria-targeting drugs are promising for the pharmaceutical industry. In this work, we investigate the efficacy of compounds whose structure is composed of a mitochondrial phosphonium anchor linked to a carbon chain. These substances are capable of incorporation into the mitochondrial membranes. The work not only focuses on comparing the potency of the tested substances in selected model organisms, but...
Iron metabolism of parasitic amoeba Acanthamoeba castellanii
Krejbichová, Lucie ; Mach, Jan (advisor) ; Horáková, Eva (referee)
Acanthamoeba castellanii, an opportunistic parasitic amoeba, rarely causes infection in humans. Yet, the treatment and diagnosis is often complicated. It causes two diseases: granulomatous encephalitis and acanthamoeba keratitis (inflammation of the cornea of the eye). There is a modern way to deal with parasitic protists and other infectious diseases - interference with iron metabolism. Iron is a crucial element for living organisms; it is involved in many metabolic processes. However, it is also toxic for the organisms and therefore, its uptake, storage and homeostasis in the cell is a strictly controlled process. Ferric reductase takes part in iron homeostasis inside the acanthamoeba cell. Fluorescent microscopy has shown that ferric reductase is located in the vacuole membranes and its activity increases when iron availability decreases. This protein probably mediates the reduction of iron ions that are used in other cellular processes, especially for the transport of iron across membranes. Interestingly, the reduced availability of iron doesn't affect the mitochondrial Fe-S cluster pathway as we proved by fluorescent microscopy with an antibody against one of the proteins of this pathway (IscU). Iron deficiency doesn't affect respiration but alternative oxidase activity is reduced. Moreover, deprived...

National Repository of Grey Literature : 44 records found   1 - 10nextend  jump to record:
See also: similar author names
22 MACH, Jan
18 MACH, Jiří
10 Mach, Jakub
1 Mach, Jaroslav
2 Mach, Jindřich
18 Mach, Jiří
2 Mach, Jonáš
1 Mach, Josef
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