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QoS simulating of wireless network in OPNET
Rágula, Michal ; Molnár, Karol (referee) ; Vlach, Jan (advisor)
The aim of this bachelor work is project and simulation of wireless network with the Quality of Service (QoS) support. It is composed of two parts, the theoretical and the practical one. In the first part there are analyzed findings needed for understanding the existing problems. The second part describes project in detail and setup of network. The theoretical part presents division and format of wireless networks, especially Wi-Fi settings, which is defined by IEEE 802.11 standard. The biggest attention is paid to IEEE 802. 11e standard, which improves the so-called Media Access control MAC (sublayer of line layer) with extension of the QoS (Quality of Service) support in wireless local networks. This standard is important for delay sensitive applications like Voice over Wireless IP and current multimedia. The practical part describes design of wireless network in Opnet Modeler settings and configurations of particular components for the right utility. The required quality of service (QoS) support is achieved by authorization of HCF (Hybrid Coordination Function) protocol at all operating stations and AP access points. It was necessary to make two models. First of them supported the QoS service. On the other hand the same service was inaccessible for the second one. Statistics are made by comparison of final graphs of these two models.
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Application of Game Theory to Oligopolistic Market
Nekola, Jan ; Dlouhý, Martin (advisor) ; Rada, Miroslav (referee)
This work demonstrates the use of models of game theory to oligopolistic market. It is based on the models of Bertrand, Cournot, Stackelberger, which solves, how optimally the companies decide to maximize their benefit. There is illustrative example here, which is an extension of these models. Decision-making takes place in the several stages, where strategy is suggested according to the certain rules of game theory. Second example deals with the signaling game. My goal is to introduce the theory to the illustrative example and show, how games are sensitive to the changing of information or how specific factors affect the game.
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Market making jako obchodní strategie
Čamaj, Matej ; Stádník, Bohumil (advisor) ; Fičura, Milan (referee)
The purpose of this thesis is to analyze market making trading strategy and explore possibilities of using such strategy for intraday trading on the markets with the limit order book. In theoretical part we prove profitability of specified market making strategy under certain assumptions and moreover analyze effect of change of parameters on the performance of the strategy using one dimensional stochastic processes. Next the assumption of constant fair price is relaxed which leads to deterioration of profitability of these strategies. Because one dimensional stochastic processes do not capture price creation in the real world, we propose stochastic model of intraday trading in the next chapter. Advantage of this approach is that we can observe state of the limit order book during whole trading session and therefore better simulate conditions for test of the strategies. Although proposed model exhibit many phenomenons observed in empirical data like volatility clustering, in some situations it produces unrealistically high spread caused by the construction of the model, because arrivals of market and limit orders are modeled as independent processes. Another disadvantages are need of relatively extensive data for model calibration and high sensitivity of model to change of parameters. Lastly we test three different market making strategies under different choice of model parameters and show that expected profitability is positive in all cases.
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Communication Strategy of the Temelín Nuclear Power Plant
Nekovářová, Lenka ; Král, Petr (advisor) ; Vokáč, Matěj (referee)
The thesis deals with the communication of the Temelín Nuclear Power Plant. Nuclear sources of energy are currently a sensitive issue. The planned extension of the Temelín NPP is also a frequently discussed topic in the Czech Republic; it affects many stakeholders and faces increased public and media interest. The goal of the thesis is to create a communication strategy of the Temelín NPP targeted to the public. The draft of the strategy is based on the conclusions of the SWOT analysis focused on the Temelín NPP communication, furthermore, on the analysis of selected online communication tools used by the Temelín NPP, and mainly on the quantitative survey conducted with the purpose to find out the actual public perception of the Temelín NPP and the public attitude to nuclear energy in general. The strategy also uses the knowledge of the company ČEZ media analysis and the findings of the sociological survey among inhabitants of the area close to Temelín compiled by STEM/MARK research agency.
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Gesture and physical gesture
Pecová, Kateřina ; Hůla, Zdenek (advisor) ; Velíšek, Martin (referee)
Title: Gesture and physical gesture Summary: "Everything is connected with everything" on the basis of this sentence in the thesis of human gestures seeking connection with the outside world. Master's thesis in the first part highlights the importance of sensory perception, as one of the major sensitive sensor that uses our surroundings, by extension, our world is to communicate with us. In addition, point out the intellectual transformation of sensory perceptions, as the second phase of human perception. I thought the transformation of the image formation of a subjective reality seen and then selecting the appropriate medium to share with other people. The work and the choice of media is used for our gesture, whether it is a gesture in the position, time, space or matter. Teaching with artwork based on this concept. Keywords: World, soul, think, culture, gesture, picture
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Monitoring of immune parameters during anti-tumor immunotherapy
Bílková, Pavla ; Palich Fučíková, Jitka (advisor) ; Fialová, Anna (referee)
Dendritic cells are the most effective antigen presenting cells in humans, they stimulate naive T lymphocytes and thus initiate specific immune response. The discovery of dendritic cells and understanding of their functions contributed to the idea of usingdendritic cells for the treatment of cancer. Anti tumor immunotherapy is a therapeutic strategy that aims to induce and maintain immune responses against tumor cells. Currently, immunotherapy based on dendritic cells has strong position among other anti cancer therapies and seems to be a promising therapeutic option for patients with tumors. In this work, I evaluated the effectiveness of treatment in patients with prostate cancer treated with immunotherapy based on dendritic cells. I focused on the detection of antigen specific T lymphocytes in peripheral blood against tumor antigens, PSA, NY ESO 1, MAGE A1 and MAGE A3. Using a 3 day standard protocol for the detection of antigen specific T cells using intracellular cytokine staining we were able to detect only a small percentage of this minor population. Only after extension of the protocol, we increased the sensitivity setting and we detected a significantly increased frequency of antigen specific T lymphocytes in the peripheral blood after one year DC vaccines application.
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The role of Prp45p in mRNA expression and maturation
Abrhámová, Kateřina ; Půta, František (advisor) ; Pichová, Alena (referee) ; Janderová, Blanka (referee)
Prp45p of Saccharomyces cerevisiae and Snw1p of Schizosaccharomyces pombe are essential proteins, which share extensive homology with the mammalian transcription regulator and splicing factor SNW/SKIP. We have analyzed the essential function of these proteins in both yeasts and found a mutation (prp45(1-169)) that exhibited temperature sensitivity. The mutant strain harboring the corresponding chromosomal deletion shows temperature sensitive phenotype and hypersensitivity to cycloheximide, hydroxyurea, calcofluor white, and to microtubule inhibitors. At 30řC, the cells are often elongated, deformed, and larger than wt. After synchronization prp45(1-169) cells stop their growth with 2N DNA content at 37řC. We found that the temperature sensitivity is not overcome and the hypersensitivity to microtubule destabilizing drugs is only partially suppressed by the excision of intron from TUB1 gene. This distinguishes prp45(1-169) from those splicing factor's mutants that cause tubulin-dependent G2/M arrest, which can be relieved by the expression of intronless tub1. We performed analysis of splicing in vitro and found that splicing of optimal substrates is not impaired. We also compared the content and stability of RNA in wt-cells and in prp45(1-169) cells at variol temperatures using microarrays. The...
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The Role of Gut Microbiota and Lipopolysachaide Content of the Diet in the Development, Maturation and Function of the Immune System
Hrnčíř, Tomáš ; Tlaskalová - Hogenová, Helena (advisor) ; Prokešová, Ludmila (referee) ; Macela, Aleš (referee)
Mammals are essentially born germ-free but the epithelial surfaces are promptly colonized by astounding numbers of bacteria soon after birth. The most extensive microbial community is harboured by the distal intestine. The gut microbiota outnumbers ~10 times the total number of our somatic and germ cells. The hostmicrobiota relationship has evolved to become mutually beneficial. Studies in germfree mice have shown that gut microbiota is essential for the proper development of the immune system. The pivotal role of the innate immune system in the complex and dynamic host-microbiota interactions has become increasingly evident. The principal aims of the present study were: firstly, to determine whether LPS-rich sterile diet can promote maturation of the immune system in germ-free mice, secondly, to elucidate whether gut microbiota and LPS-rich sterile diet influence the LPS susceptibility, and finally, to investigate a role of the adaptive immunity in endotoxin shock. Our data clearly show that both live gut microbiota and LPS-rich sterile diet increase susceptibility to endotoxin shock. Further, we demonstrate that immunodeficient SCID mice, which lack mature B and T cells, are more sensitive to endotoxin shock than immunocompetent Balb/c mice. In addition, we show that not only live gut microbiota but also...
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Microscale flow and diffusion characteristics inside the urban area
Kukačka, Libor ; Brechler, Josef (referee) ; Jaňour, Zbyněk (advisor)
Dispersion of pollutants in the atmosphere of urban areas is the important task in environmental sciences. The main goal was the quantitative studying of a flow in a symmetrical urban built-up area depending on a running on flow direction. There was given a theoretical base of the physical modeling of the atmospheric boundary layer in this work. The measurement was done with a model of the urban build-up area at a scale of 1:200 in the aerodynamic wind tunnel of the IT AS CR, v.v.i. in Nový Knín. The requirements to the similarity to a real atmospheric boundary layer and a model boundary layer in the wind tunnel were accomplished. Velocity fields, concentration fields and fields of scalar uxes were measured in the area of a rectangular intersection. Results of the experiment showed significant sensitivity of the velocity field about the running on flow direction that extensively inuenced ventilation of street canyons.
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