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Macromycetes of Řevnice neighborhood
Marhoulová, Eliška ; Novotný, Petr (advisor) ; Skýbová, Jana (referee)
This thesis is a summary of basic information about macromycetes, their particular way of life and identification cognitive characteristics. It also includes processing research areas Hřebeny - Brdy between Dobřichovice and Řevnice. The research results are applied in primary school instruction. As a part of this work is the proposal excursion to this area and trail design. Key words: macromycetes, mushrooms, Brdy
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Analysis of glucans in plant and microbial samples
Vít, Radek ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The aim of the diploma thesis is study of glucans production in selected species of yeasts, algae and plants. Cultivation conditions for yeast strains were performed to gain increased production of glucans under different temperature conditions and in media of different composition. Into the set of tested yeasts species strains Saccharomyces cerevisiae (CCY 6646), Sporidiobolus pararoseus (CCY 19-9-6), Phaffia rhodozyma (CCY 77-1), Rhodotorula glutinis (CCY 20-78-26) and Cystofilobasidium infirmominiatum (CCY 17-18-4) were enrolled. Saccharomyces cerevisiae was cultivated as a control strain because of its verified production of -glucans. -glucans were determinated by the enzyme kit K-YBGL Megazyme. For comparison, algal strain Euglena gracilis (CCALA 349), some species of mushrooms (shiitake, oyster mushroom, garden champignon and Jew’s ear) and cereals (wheat, rye, oats, rice and barley) were analysed too. Further, fatty acid content in the yeast cells was determined by the GC/FID. The best producer of yeast -glucans was R. glutinis CCY 20-7-26, which showed the highest biomass production (12-14 g/l) and also a relatively high amount of -glucans (25-30 %), in cultivation at 15 °C in a medium containing yeast extract in combination with ammonium sulphate. The presence of -glucans has been demonstrated in the microalgae, Euglena gracilis CCALA 349, as well as in samples of higher fungi and cereals.
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Macromycetes of Řevnice neighborhood
Marhoulová, Eliška ; Novotný, Petr (advisor) ; Skýbová, Jana (referee)
This thesis is a summary of basic information about macromycetes, their particular way of life and identification cognitive characteristics. It also includes processing research areas Hřebeny - Brdy between Dobřichovice and Řevnice. The research results are applied in primary school instruction. As a part of this work is the proposal excursion to this area and trail design. Key words: macromycetes, mushrooms, Brdy
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Mercury contents in mushrooms growing nearby Horní Slavkov
Říhová, Štěpánka ; Svoboda, Lubomír (advisor) ; Tondl, František (referee)
In the seventies it was discovered, that many species of wild higher fungi accumulates in their fruiting bodies some potentially hazardous elements in amounts significantly higher, than any other ecosystem cells, respectively eatables. For the most risky elements especially cadmium and mercury are considered. The aim of my rigorous paper was to determine mercury content in the fruiting bodies of mushrooms growing in the area with an extremely rich history of mining and processing of metal ores (mostly tin). Mushroom samples were collected (from July 2005 to September 2006) from two sites near the village of Krásno close Horní Slavkov. Mercury was analyzed in 130 samples of 13 edible mushroom species. Only fully developed fruiting bodies were selected for the analysis, each represented a separate sample. It was also carried out a comparative test, detecting effect of fungal cleansing on the kontent of mercury. Mercury was determined in homogenized samples on a single-atomic absorption spectrometer AMA -254, which uses the technique of atomizing cold vapor generation of mercury with subsequent capture on a gold amalgamator. The content of mercury in the fruiting bodies of the monitored area was very well comparable to the normal state in areas without mining. In point of harmful character of mercury,...
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Mashrooms and their importance in nutrition.
SOUČKOVÁ, Jana
The thesis is focused on the theme of mushrooms and their importance in nutrition. The theoretical part deals with the chemical composition of higher mushrooms in general and describes their significance for human health. It focuses mainly on the positive effects of mushrooms on the human body. The practical part consists of questionnaires on the subject and their evaluation. The aim of my work was to find out what knowledge about mushrooms people have and how often they consume them.
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Image Object Rcognition Using Android Platform
Šimon, Martin ; Zahrádka, Jiří (referee) ; Zemčík, Pavel (advisor)
This thesis describes an object recognition issue in connection with the Android mobile platform. It analyzes existing methods and procedures from the field of the object recognition as well as previous solutions of a similar topic. It builds on these basics and proposes a handy application which aspires to become a powerful assistant during mushroom picking. It considers the target device, which is memory and power limited, during the design and implementation phase. The results are evaluated by several experiments including an user experience. The implementation of the application itself is a part of this thesis.
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The presence of pollutants in matrices of bioindicators
Vlček, Ladislav ; Vávrová, Milada (referee) ; Doležalová Weissmannová, Helena (advisor)
This master‘s thesis concerns with occurrence of heavy metals in the environment, mainly in the matrices of bioindicators. Special attention is given to occurrence of mercury in the environment and its quantification in bioindicators. Mercury and its compounds are today considered as one of the most significant contaminants in the environment. Some sorts of mushrooms cumulate sizable amounts of mercury in their fruiting body and therefore we can use them to consider loading of different areas. Eight areas in Vizovice and its surroundings were chosen for these purposes and during the years 2006 – 2008 harvested 50 samples from 19 sorts of mushrooms from there. The concentrations of Hg were determined in all samples by single-purpose absorption spectrometer AMA 254. Comparison of the mercury content between individual sorts of mushrooms in the same area as well as comparison of loading of areas was performed and also bioaccumulation ability of individual sorts of mushrooms was determined.
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Toxic compounds of mushrooms
Ascherová, Adriana ; Vitoulová, Eva (referee) ; Vítová, Eva (advisor)
This work is devoted to the toxic compounds of mushrooms. The theme is divided into three sections. The first part deals with the chemical composition of mushrooms, with main emphasis on the content of heavy metals and medicinal substances. The following section is focused on the poisonous mushrooms, the most important representatives and the mechanism of action of their poisons. Part three explains the issue of edible mushrooms and the poisonings, which may cause. In particular they are biogenic amines, the work monitors their chemical composition, origin, structure and distribution, occurrence, biological effects and methods of measurement (gas chromatography, liquid chromatography, thin layer chromatography and capillary electrophoresis).
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Contents od caesium-137 in mushrooms
ČADOVÁ, Michaela
The aim of this work is to compare the presence of Cs-137 in different kinds of mushrooms in selected locations using semiconductor gamma-ray spectrometry. Research question was determined: Does Cs-137 content amount vary depending on the fungal species? The first part deals with basic knowledge of ionizing radiation, which is important for the complex understanding of thematic problems. This concerns basic radiobiological terms such as radioactivity, ionizing radiation, its biological effects and basic units and quantities used in radiobiology and radiation protection. Further I deal with the above mentioned measured radionuclide Cs 137, which resources in our country include the Chernobyl nuclear plant accident and tests of nuclear weapons in the 20th century. I also pay attention to this element in the cycle of nature, its distribution in the body and the effects on our environment. Shortly I even deal with basic knowledge of the Cs-137 accumulation in mushrooms. Another point mentioned is the research methodology, a brief introduction to semiconductor gamma spectrometry. As sampling sites I selected Zadov, Churáňov and Kvilda in Šumava, where in 1986 there was detected the biggest radiation fallout. For comparison, I also measured samples of mushrooms from localities of less significant fallout. These are sites around a village of Příbraz and in the woods of Jemčina within Třeboň region. A total of 26 mushroom samples were taken. Mass activity was measured by semiconductor gamma spectrometry in the Regional Center of the State Office for Nuclear Safety in České Budejovice. In addition to Cs 137 there was also measured a content of a natural isotope Potassium-40. It is a Cs 137 chemical homologue, which has similar properties and is present homogeneously throughout the whole ecosystem. For comparison I also measured mass activity of other forest ecosystem components such as moss, bark, cones and ground. Measuring time of the mushroom samples was for about 24 hours. The other forest ecosystem components measuring time ranged from 24 to 48 hours. The measured spectra were analysed by using the GAMAT software tool. The highest measured mass activity of Cs-137 was detected in a pine bolete sample from the site of Churanov in Sumava (4 265 Bq.kg-1). Also Russula emetica from Zadov shows relatively high activity (3 050 Bq.kg-1). The smallest amount of Cs 137 was measured in Cystolepiota high, originating in the locality of Jemčina in Třeboň region (5.6 Bq.kg-1) and in a sample of Lactarius volemus from the nearby village Příbraz (34.7 Bq.kg-1). The highest mass activity of natural radionuclide K-40 was measured in a sample of blusher from the village Příbraz site (4 329 Bq.kg-1). High value was probably caused by the use of fertilizers or pesticides near this sample. Large number of K-40 was also seen in Laccaria amethystina, also coming from the village Příbraz (2 901 Bq.kg-1). On the contrary, the least of this element was found in Cystolepiota high sample from Jemčina forests (971.8 Bq.kg-1) and in Lactarius volemus from Příbraz (999,6 Bq.kg-1). According to the measured values, we can conclude that the measured mass activity differs a lot. Individual sites may have different values because of uneven rainfalls in our country at the time of the radioactive cloud passage after the Chernobyl explosion. However, specific species of fungi from the same sites differ, which is due, inter alia, to soil characteristics and mycelium depth. The results correspond to the fact that the mushroom-shaped fungi show a greater amount of Cs-137 unlike gilled fungi presented as well by other authors.
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