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Characterization of eukaryotic translation initiation factor 3 subunits (eIF3) in A. thaliana male gametophyte
Linhart, Filip ; Hafidh, Said (advisor) ; Retzer, Katarzyna (referee)
From RNA-to-protein, translation initiation and protein synthesis is mediated by trans-acting factors that recognize mRNA features common to almost all eukaryotes. Eukaryotic translation initiation factor 3 complex (eIF3) is a highly conserved protein complex that recognizes 5'-CAP elements of the mRNA to initiate translation. eIF3 consists of nine subunits, three of them having two isoforms: eIF3A, eIF2B1, eIF3B2, eIF3C1, eIF3C2, eIF3D, eIF3E, eIF3F, eIF3G1, eIF3G2, eIF3H and eIF3K. This work deals with functional characterization, expression and subcellular localization of eIF3B1, eIF3B2 and eIF3E in Arabidopsis thaliana male gametophyte and interaction of eIF3E with the Constitutive photomorphogenesis 9 (COP9) complex as a regulatory complex of eIF3E post-translational control. Here we show that depletion of eif3b1 or eif3b2 is not gametophytic lethal and that the two protein might function redundantly, whereas, knockout of eIF3E causes male gametophyte lethality. Interestingly, eif3b1 show post-fertilization defects during embryogenesis, suggesting that its redundancy with eIF3B2 is restricted to the gametophyte. Gene expression studies revealed high expression of eIF3 subunits in actively dividing zones of leaf primordia, root meristem and root elongation zones as well as in the vegetative...
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The issue of the revision corrected wiring residential building
Štefek, Roman ; Janda, Marcel (referee) ; Veselka, František (advisor)
Bachelor’s thesis deals with the verification of electrical installations in residential buildings. The task of the thesis is to get acquainted with the requirements of the regulations with a focus on Czech technical standard CSN 33 2000-6, CSN 33 1500 and follow-up. Specifically, the information gathered is used to the implementation of the review installations in renovated apartment building. The aim is to apply the theoretical knowledge and information for the practical verification of the electrical installation.
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Analysis and design of the replacement of the specific overhead line in distribution network
Račuch, Marek ; Bátora, Branislav (referee) ; Ptáček, Michal (advisor)
The diploma thesis deals with the issue of the reconstruction of outdoor cables of low voltage distribution network in exchange for the cable line. It analyzes the important factors which are necessary for the preparation of the project documentation for the issue of the territorial consent. The thesis is divided into 4 main chapters. After the first chapter there is introduced chapter 2, which shows the differences between cable and outdoor cables with their advantages and disadvantages. Chapter 3 deals with the guiding principles for low-voltage cable design and the types of equipment needed for the proper operation of a particular distribution network while respecting the requirements of valid PNEs and ČSNs. Chapter 4 explains the chronology of the process leading to the formulation of a basic technical proposal, which is further addressed in Chapter 5, which deals with the complete formulation of the project documentation for the issue of territorial consent.
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Proposal of electrical equipment for soaking and drying cores for making castings
Čermák, Jiří ; Hájek, Vítězslav (referee) ; Veselka, František (advisor)
This diploma thesis deals with proposal of electrical equipment for soaking and drying cores for making castings at foundry in a Great Britain. It brings look at system of technical norms ČSN and it compares it with british system of technical norms British standards. The second part of this thesis contains detailed proposal of this equipment, including specification of control system, specification of power supply cables and control cables and particular technological units. The whole project documentation is enclosed as an inseparable attachment of this diploma thesis.
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Sheet thickness optimization of the bolted steel tank
Konečný, Jiří ; Lyčka, Petr (referee) ; Skalka, Petr (advisor)
This thesis deals with bolted steel tanks, especially modification one of them. This modification is realized in cooperation with Kohimex spol. s r.o., which is assembling theese tanks for many years. The thesis continues on the bachelor one called "ASSEMBLED STEEL TANKS", which has already dealt with this issue. At the beginning of the thesis the issue of tanks is explained. Furthermore, there is made description of the tank modification details. At the next stage, the work presents stress-strain states of the tank, first for the current tank and then for the modified option. On the basis of completed analysis is made economic evaluation of design modified tank option due to the current tank. The results of this study allow better issue understanding of bolted tanks designing or they give impuls to improve the current way of designing.
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Polyfunctional Building
Holub, Ondřej ; Kopřivík, František (referee) ; Košíčková, Ivana (advisor)
Diploma Thesis „Polyfunctional building“. The work deals implementation project of polyfunctional building in Jihlava. Object is situated on Jihlava suburb in emerging part called „Horní Kosov“. Land is gently sloping from west to east. It continues for Buková street. The building has six floors and a basement. The basement is made from monolithic reinforced concrete. It is used as garages. Floors are made of ceramic bricks with isolation . First floor contains four commercial premises and main entry for housing part. Seventeen flats are situated from second to sixth floor. Sizes of apartments are 1+kitchen corner to 4+kitchen corner with terraces and balcony. Roofing is solved with simple flat roof. Heating will be ensured with central heating. Every apartment, commercial premises, shared premises are going to have it’s own gas boiler. Polyfunctional building is connected on a local road – Buková street, which is connected to new communication. Within multifunctional building twenty parking slots is designed at the basement. Next fourteen parking slots is designed outside.
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Geodetic Works During Reconstruction of Road Crossing in Uherské Hradiště
Lovecký, Marek ; Sláma, Jiří (referee) ; Bureš, Jiří (advisor)
This thesis is engaged in geodetic works related to the increase of the capacity of the road II/497 and the modification of the intersection with the road I/55 in the town of Uherské Hradiště. The work is mostly focused on the field of the engineering geodesy. It resolves the issues from the alignment during the construction to the focus of the construction itself. Individual survey procedures are analyzed in the context of the prescribed deviations specified in the project documentation or ČSN. Geodetic documentation for the selected measurements is worked out.
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Active database of standart at Institute of design created
Slezák, Jan ; Zimmerman, Martin (referee) ; Dvořáček, Jiří (advisor)
Aim of this bachelor’s thesis is to create compact database of valid standards, which are on hand the Institute of design created with the possibility continuous refilling by database system. A short charakteristics of standard and basic identification data belong to the survey. There is charakteristic of standard, kinds of standards and history of standards in the first part. Creation of standards, their numbering and obligation are introduced in the second part. Databases are introduce in the third part.
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Metal welding and weld testing according to new norms ISO, EN and consequential ČSN
HAJNÝ, Tomáš
Work considers kind of materials joining via welding. At the begining the bachelor work considers history of this branch. Then there are norms and regulations ISO, EN and CSN for welding. Next part describes welding methods and weld tests. The last part is focused on common technology of welding. Ambition of this bachelor work is to create learning text and briefly show to the students, which are the methods and kinds of welding and welding tests and possibility how to use this methods in practice. Here are methods described theoretically and also examples from practice. This bachelor work can be used at pedaggical faculty for teaching this topic.
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