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Effect of fatty acids on lipid metabolism and weight reduction
Hlavatý, Petr ; Kunešová, Marie (advisor) ; Müllerová, Dana (referee) ; Flachs, Pavel (referee)
Fat tissue plays a main role in the development of the metabolic syndrome. Many components of the metabolic syndrome may be improved by dietary arrangements, including an increased intake of n-3 PUFA. In addition to the positive effect of n-3 PUFA, a possible effect of a higher calcium intake on influencing weight loss and energy metabolism has also been discussed for a long time. In addition to nutritional factors, genetic factors significantly contribute to influencing weight and lipid and glucose metabolism. In study A, 40 obese women were observed during three weeks of weight reduction management. Women were randomly divided into two groups. The first group received yogurt enriched with n-3 PUFA, and the second group consumed yoghurt without the supplementation. The results show that low-dose supplementation with n-3 PUFA in yogurt in combination with a reduced energy intake increases n-3 PUFA content in serum lipids and prevents adverse changes in the composition of FA in serum after a short-term low-calorie diet. In study B, we monitored the influence of n-3 PUFA supplementation on short-term weight management with VLCD in 20 women with severe obesity, who were randomly divided into two groups. The first group received VLCD enriched with n-3 PUFA, the second group VLCD with a placebo. The addition of...
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The effect of iodine on functional parameters of ewes.
DUŠOVÁ, Hana
The aim of this work was to assess the long-term effect of excessive iodine intake on thyroid activity and selected immunological, haematological and biochemical parameters in blood of ewes and their lambs. The experiment was conducted from August 2009 to February 2010 in an experimental barn of Faculty of Agriculture, University of South Bohemia in České Budějovice. Into the experiment were used Šumava sheep breed ewes and their born lambs. Group A (control) consisted of 6 ewes after lambing was extended to 7 born lambs, group B (experimental) 6 of 6 ewes and lambs. Lambs were put into the experiment from the 1st to the 60th day after birth and received iodine in the milk of their mothers. Iodine supplementation to the diet of ewes was launched during the first to second months of pregnancy and finished 60 days after birth. During the experiment, a group of ewes A received 3,1 and group B 5,1 mg iodine per kg of dietary dry matter in the form of calcium iodate. During the experiment was regularly collected blood of ewes (before lambing and 1st, 10th, 30th and 60th day after birth) and lambs (1st, 3rd, 10th, 30th and 60th day after birth) to determine the concentration of thyroid stimulating hormone and immunoglobulin G (ELISA) in blood serum, thyroid hormones (RIA), total protein, urea and alkaline phosphatase activity in the blood plasma (biochemical analyser), the content of haemoglobin, white blood cell´s and red blood cell´s counts and haematocrit values in blood (haematological analyser), percentage of albumin, -1-globulins, -2-globulins, -globulins, and -globulins in blood serum (electrophoresis) and the content of iodine in the blood plasma, urine and milk of ewes (colorimetric method Sandell-Kolthoff). During the experiment were not observed in ewes and lambs clinical symptoms associated with an excessive intake of iodine. In experimental group of ewes with iodine intake 5,1 mg/kg of dietary dry matter were found higher values of thyroid stimulating hormone, lower values of free triiodothyronine, free thyroxine, IgG, white blood cells, -1-globulins, -2-globulin and -globulin, significantly lower values of -globulins after parturition, significantly higher values of urea and alkaline phosphatase activity. The iodine content in blood plasma, milk and urine of ewes is increased in direct proportion to its concentration in diet. Lambs of mothers with iodine intake of 5,1 mg/kg of dietary dry matter were significantly higher values of thyroid stimulating hormone from the 10th day after birth, significantly lower values of free triiodothyronine and free thyroxine first day after birth, immunoglobulin G and -globulins in 30th day after birth, lower (statistically significant in middle samples) values of total protein and white blood cells, higher values of urea and alkaline phosphatase activity. The weight gains of lambs were not significantly affected by iodine supplementation. These results in ewes with iodine intake of 5,1 mg/kg dietary dry matter and especially their lambs drawn to risk of reduce the activity of the thyroid gland and the negative impact of parameters of humoral immunity, mainly IgG and -globulins.
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Supplementations of the Mallard and their impact on wild populations
Pechmanová, Hana ; Musil, Petr (advisor) ; Kreisinger, Jakub (referee)
The Mallard (Anas platyrhynchos) is quite a popular game bird species. In many states, particularly in Europe and North America, captive-rearing and releasing of mallards has become common practice in wildlife management. The aim of these supplementations is to boost the size of hunted populations. More than three million Mallards are released each year. This paper aims to summarize and assess the potential effects of captivity-reared Mallards on the wild populations. Supplementations of mallards pose a threat to the genetic identity and variability of wild populations and may potentially lead to morphological and behavioral changes. Breeding facilities can be source of many pathogens, so supplementations may lead to transmission of these pathogens into wild populations. All the factors mentioned above may thus affect the fitness and population dynamics of wild Mallards. This paper also briefly considers how the releases can affect other species and the environment. This issue is not well studied so more studies and appropriate monitoring are needed for an assessment of the rate of these processes.
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Success of reintroduction and supplementation programmes and behavioural changes in captive birds and primates.
Janovcová, Markéta ; Landová, Eva (advisor) ; Kůs, Evžen (referee)
The aims of this study are to: 1) review and evaluate the data available in scientific literature about reintroduction and supplementation programmes in birds and primates. The success of founder population reintroduction into the nature was assessed by its population size, survival rate and other life history traits. The breeding in captivity vs. using wild-caught animals for reintroduction and breeding conditions (longevity of captivity) would be checked. Breeding in enclosure versus semi-captive conditions was also checked. 2) mainly, assess behavioural and morphological changes of populations in relation with captive breeding in relation to success of reintroduction. 3) Map the geographic distribution of reintroduction programmes of particular higher taxa and evaluate the IUCN species status of animals used for conservation programmes.
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Cartilage regeneration using peroral supplementation and artificial scaffolds
Filová, Eva ; Amler, Evžen (advisor) ; Jebavý, Lukáš (referee) ; Otáhal, Stanislav (referee) ; Tománková, Kateřina (referee)
Cartilage can regenerate only in a limited extend. The aim of the study was to enhance hyaline cartilage regeneration using peroral supplementation of glycosaminoglycans (GAGs) and antioxidants, or using artificial scaffolds seeded with autologous cells. Scaffolds seeded with chondrocytes or mesenchymal stem cells (MSCs) have a potential for treatment of cartilage defects. For the complex characterization of biomechanical properties of scaffold, we developed the novel shock dynamic method. Subsequently, the composite hyaluronate-type I collagen-fibrin scaffold with the viscoelastic properties and dynamic loading response similar to the native cartilage was developed. The composite scaffold was then used for osteochondral regeneration and physeal cartilage regeneration. The effect of peroral supplementation of GAGs and antioxidants (vitamin E/selenium) on the regeneration of osteochondral defects was investigated in rabbits. After introduction of defined osteochondral defects in the knee joint, groups of ten animals were given a GAG/vitamin E/selenium mixture or a placebo (milk sugar) for 6 weeks. The amount of sulfated GAGs in the osteochondral regenerates was significantly higher in the GAG group. In both groups, the GAG amount in the cartilage of the operated knee was significantly higher than in the...
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Food supplements and iodine supplementation in pregnancy
VAVROUŠKOVÁ, Edita
Iodine is a trace element tremendously important for thyroid gland hormone production. It affects normal evolution of brain, especially in the time of intrauterine development and in the first year of child's life. Deficiency of iodine can lead to creation of pregnancy goiter, it also decrease the probability of carrying fetus to term and increase the probability of disorder in psycho-motoric evolution of child. Czech Republic is geographically lined up among the regions with lack of iodine in natural sources and food. However, nowadays the iodine deficiency is considered to be managed. But risk groups of population still exists, especially pregnant women can be endangered by lack of iodine, because the need of iodine intake in pregnancy increases to 200 250 ug per day and this level must be ensured. Consumption of food rich in iodine can cover the increased need of iodine. Significant source of iodine is milk and milk products. Another source is table salt enriched with iodine, but its daily intake is limited. Rich sources of iodine like sea fish, sea products and seaweed rarely occur in our diet. The goal of thesis was to evaluate intake of dietary supplements with iodine by pregnant women and evaluate women's awareness of higher iodine need and iodine food sources. Quantitative research in form of questionnaires was chosen for getting information from higher number of individuals. The questionnaire consists of 15 questions. Answers were gained from 118 respondents living in Central Bohemia, Moravia-Silesia region, Liberec region, region Highlands and South Bohemia. Questionnaire was distributed to gynecological ambulances by employees of State health institution. I visited gynecological ambulances in South Bohemia personally. By evaluation of questionnaire was found out that dietary supplement with iodine use 63 % pregnant women in researched population. All respondents always use only one type of dietary supplement. Among the most used belong "Chytré miminko", "GS mamavit" and "Femibion 800". 68 % of respondents know the importance of higher iodine intake in time of pregnancy. Question about daily recommended dosage was correctly answered only by 35 % of respondents. As a food sources of iodine the pregnant women know sea fishes, sea products, seaweed and table salt enriched with iodine. But 90 % of respondents are not familiar with milk and milk products as important source of iodine. Pregnant women should be better informed about importance of sufficient supply of iodine and about sources of iodine by gynecological ambulances. Information about mentioned issues should occur in magazines, education materials and internet articles more often. Respondents were notified of issues and important role of iodine in time of pregnancy by questionnaire. It is very probable that women found correct answers on the internet or in publication for expectant mothers after filling in the questionnaire.
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Food supplements and iodine supplementation breastfeeding women
IVANOVÁ, Markéta
Iodine is a trace element that is essential for the production of thyroid hormones triiodothyronine and thyroxine. Iodine deficiency had in the past result in many serious diseases and disorders. At present is in the Czech Republic considered mastered. However, Iodine deficiency are vulnerable to specific groups of the population, including pregnant and nursing women, because of their need and the recommended daily dose of iodine is increased. Breastfeeding women should receive adequate amounts of dietary iodine for optimal saturation for themselves and their child. Otherwise, exposing themselves and their child risk of diseases and disorders of iodine deficiency. Increased supply of iodine can be replaced by eating foods rich in iodine. In the event that you cannot increase intake of iodine - rich food preferences in iodine, there are various supplements that contain iodine. The aim of this thesis was to evaluate the intake of iodine supplements and supplements in nursing women and breast-feeding women to assess the awareness for greater needs of iodine and food iodine sources. There were set up the three research questions: How many nursing women used to supply iodine supplements and supplements?, What is the awareness of breastfeeding women need more iodine? and What is the awareness of breastfeeding women about dietary sources of iodine? A quantitative research strategy was used for the implementation of the research. The method used for data collection was made by an anonymous questionnaire. A questionnaire was handed in a printed copy to pediatricians and gynecologists in the southern Bohemia. With the doctors help were invited nursing women to fulfill the questionnaire. Part of the women was invited to complete the questionnaire directly. The research sample consisted of 117 female respondents. The research shows that 62 % of breastfeeding women know why it is important to increased need for iodine during lactation. But only 9 % of women know all the risks of iodine deficiency on the mother mentioned in the questionnaire and only one new mother knows all risk of iodine deficiency for infants listed in the questionnaire. The recommended daily intake of iodine properly reported or estimated 40.2 % of women. It turned out that 46 % of respondents from the research group used food supplements and supplements containing iodine. Each of these respondents used only one preparation with iodine. The most commonly used products were: GS mamavit, a Smart baby and Femibion 400 and Gravital. 43 % of respondents had used a dietary supplement containing iodine from the beginning of pregnancy and in 57 % of the respondents were informed about food supplements through a doctor (gynecologist, pediatrician). The results showed that awareness of breastfeeding women about food sources of iodine is not good. 67 % of women know that settlement higher needs iodine during lactation is possible by eating foods rich in iodine, but all food sources of iodine in the questionnaires (sea fish, seafood and seaweed, iodized salt, and milk and dairy products) registered only one respondent. 39 % of women known as a rich source of iodine, seafood, seafood and seaweed, iodized salt , but none of them knew that the rich and a good source of iodine is also milk and dairy products. Based on the results of the work would be desirable to issue educational print disabled educational material that could be used to increase awareness of not only women who are breastfeeding, pregnant women as well as the higher of the recommended daily dose of iodine and what foods to include in your diet in order to pay the increased intake of iodine. Provide information on supplements containing iodine, which can add missing quantities of iodine, what products are available and what are the currently preferred by nursing women.
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Optimization of sport horses nutrition
MARYŠKA, Jan
This thesis deals with sport horse feeding. Suitable horse nutrition is essential for healthy and correct development of the whole horse organism. Every horse is different regarding its characteristics, work load, age, and health condition and therefore it is necessary to approach individually to its feeding. To set an optimal feeding batch so that it meets all requirements on energy, nutrients, and the right ratio of minerals and vitamins is not easy. The aim of this thesis is to analyse feeding practice in a stud farm focusing on feeding batches of sport horses. The work is divided into a theoretical part including basic descriptions of the horse digestive system, nutrients, and horse forage, and a practical part including the methodology used for the analysis, the description of the analysis when the forage used in the stud farm was compared with recommended optimal nutritious values for horses using appropriate software. The special attention is paid to the content of selenium in the feeding batches and its supplementation to horses with a deficit of this element. The thesis conclusion summarises findings of the conducted analysis together with a recommendation of optimal feeding of the horses in question.
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