|
Burnout by Nurses in Intensive Care
Jánová, Simona ; Mellanová, Alena (advisor) ; Honzák, Radkin (referee)
Burnout by nurses in intensive care Abstract: Objective of this thesis was to determine incidence of the risk of burnout by nurses in intensive care in the Motol University Hospital in Prague, compare to risk of burnout among nurses in intensive care for childern and adults and by age, sex, marital status, education, length of experience in health care and on the current department, satisfaction and performance in the workplace. The research method for data collection were two anonymous questionnaires: one prepared by author of the thesis and a standardized questionnaire by dr. Pines to determine the risk of burnout. It was found that more than hlaf of nurses in FN Motol had low risk of burnout and has not demonstrated that nurses have on children's or adult wards increased risk. Results confirmed that discontent and the increasing intensity of stress in the workplace is proportional to the risk of burnout. This research confirmed that although most nurses achieved good results has been shown a high percentage of nurses with alarmig score and even burnout. Therefore we should not ignore this issure, but rather to warn him and avoid unpleasant consequences through educational seminars, pleasant working environment and do not overload nurses. Keywords: burn-out, stress, nurses, intensive care, mental hygiene
|
|
Biodiversity of standing floodplain waters and processes of its formation and preservation
Pithart, David
Water chemistry and planktic communities in the naturally eutrophic alluvial waters in the Lužnice floodplain have been studied. The floofplain waters are naturally eutrophic, with strong vertical physico-chemical gradients and oxygen depletions at the botom. Small pools and backwaters of this type have unique phytoplankton structure. Prevailing dominance of flegellates, namely Cryptophyceae, and low proportion of green chlorococcal algae and Diatoms, and almost absence of Cyanophyceae are very unusual for such eutrophic waters. Flagellate dominate thanks their ability to keep the vertical position in a permanently light limited conditions (resulted from poorly mixed water columns and intensive shading of water surface by vegetation). Floodplain geodiversity play crucial role, determining morphology and location of pools and backwaters, their exposition to flooding and inflows of underground water. Terrestial vegetation effects light exposition, leaf litter.
|
|
Identification of tram wheel with vibro-dissipation layer
Pešek, Luděk ; Veselý, Jan ; Podruh, J.
The wheels as vibration transformation elements and noise emitters substantially contribute to the total noise level of riding vehicle. We aimed at investigation of dynamic behavior of railway tram wheel with a special construction. This wheel has a rim separated from disk by rubber cushioning. The selected dynamic parameters of the wheel are modal, frequency and damping characteristics. Proportional damping was implemented into rubber layer of FE wheel model as the first approximation of dissipation behavior.
|
|
Laboratory diagnosis of mycobacteria, with a focus on the bacteriological proof
VELKOVÁ, Martina
Laboratory methods play an important role in disease diagnostic. Although there are currently available fast molecular genetic methods and methods based on cell response, basic diagnostic method of direct evidence is still microscopy and cultivation. Microscopy (staining by Ziehl-Neelsen and fluorescence microscopy) is the primary method for most of the specimens, especially for sputum. The sensitivity of this method is not very high because for the detection of a positive finding in 1 mm3 is needed at least 105 microbes. However, the method is important because it can fastly proof extensive clinical and epidemiological serious diseases. The ?Golden standard? is today the cultivation on solid egg media. Nowadays it is filled in automatic detection system, which accelerates the time of detection. The thesis was carried out in the Laboratory of Medical Microbiology, Bacteriology department, Hospital Ceske Budejovice in the period from 1.1.2013 till 6.6.2013, and included 400 sputa, which were processed and examined. The purpose was to compare the effectiveness of decontamination methods with HCl and with NALC, and to monitor the recovery and detection for the automatic detection system and conventional cultivation. In the automatic system using the BACTECTM MGITTM 960 Non-Radiometric fluorescent technology is cultivation in liquid Middlebrook 7H9 medium supplemented with antimicrobials (polymyxin B, amphotericin B, nalidixic acid, trimethoprim, azlocilin). The classical culture used solid egg culture medium: Lowenstein-Jensen and culture medium by Ogawa. The individual results then show that the method of HCl had the overall contamination of 1 % of the samples. For each methods in 5 % BACTECTM MGITTM 960 and 4 % in the conventional cultivation. In comparison the method NALC, had the overall contamination of 21 % of the samples. For the individual methods in 26 % of BACTECTM MGITTM 960 and 50 % in the conventional cultivation. From these results it is obvious that the method with the NALC has lower efficiency, the proportion of contamination compared to the method with HCL increased by 20 %. Decontamination with the NALC method recommended by the manufacturer for BACTECTM MGITTM 960 was found to be unsatisfactory and was canceled. Comparing the recovery and the detection of the strains isolated in individual methods showed that the BACTECTM MGITTM 960 exhibits greater sensitivity than conventional cultivation, since the total of 17 strains isolated BACTECTM MGITTM 960 captured 15 against 10 strains isolated in conventional cultivation. There is also a significantly shorter time to detect positive samples. The average detection time for BACTECTM MGITTM 960 was 16.2 days, while a conventional culturing was 31 days. It was confirmed that the BACTECTM MGITTM 960 system achieves better results, but optimization is achieved by combining the two methods used. There is an apparent decrease in detection of mycobacteria from the processed statistical data of the strains isolated during the period 2010 - 2012, but a substantial reduction does not occur. Noticeable is only the decline of the isolated stains of Mycobacterium bovis BCG, which can be explained by the fact that the vaccination against tuberculosis is since 2011 no longer carried out across the board, but according to the new legislation in force only in high-risk groups. An interesting fact is that every year the highest laboratory detection is found in the age group of over 60 years.
|
|
The Separation of Gastric Aspartic Proteases Using Affinity Chromatography
Frýdlová, Jana
Human gastric juice contains mainly aspartic proteases - pepsin A and pepsin C. Both pepsins are produced by gastric mucosa as inactive pepsinogens (pepsinogen A and pepsinogen C) that differ in their physico-chemical and immunological properties. Both pepsinogens consist of molecular variants, isozymogens. Pepsinogens are activated to the corresponding pepsins in the acidic environment of the gastric lumen. The levels of pepsinogens in serum reflect the morphological and functional status of gastric mucosa. The levels of pepsinogens are associated with gastric diseases (e.g. gastric cancer, gastritis). For a diagnostic purpose, the ratio of pepsinogen A to pepsinogen C not only in the gastric mucosa but also in serum is relevant. The gastric cancer patients or the atrophic gastritis patients have significantly lower ratio of serum pepsinogen A to pepsinogen C in comparison with the healthy subjects. In addition, proportion of individual isozymogens reflects the type of the gastric disease. Determination of changes in pepsinogen levels could be useful for early diagnostics of gastric diseases mentioned above. A subject of this Ph.D. thesis is a part of a long-term investigation that focuses on the elaboration of methods for the separation of gastric aspartic proteases that would be suitable for...
|
|
The aspects of labour class housing on the example of Ostrava and Zlin
Svozilová, Barbora ; Matějů, Martin (referee) ; Czumalo, Vladimír (advisor)
The aim of this paper is to describe the housing situation of working c1ass during the transition from the society based on traditional values to the modem society of the twentieth century. Through the comparison of the housing facilities built to accomodate working c1ass people from Ostrava and Zlín, we can discover some important similarities and differences connected with approaches of the municipalities of these two settlements. Both of them chose the option to house working c1ass people on a mas s scale, but the results of their decisions ended up in two different urban formations. The examples of Ostrava and Zlín are analyzed from the interdisciplinary point of view (history, architecture, sociology and cultural studies) which provides an useful analytical tool to describe some interesting parallels and differences which emerged during their rapid development. The circumstances of their industrial boom substantially intluenced the structure and proportions of these places. The prevailing industrial character of the production was the most itluential factor which formed two unique uban areas. The text points out the key characteristic and urban features of Ostrava and Zlín and their working c1ass settlements. Powered by TCPDF (www.tcpdf.org)
|
|
ELECTORAL SUPPORT OF POLITICAL PARTIES : CHANGING PATTERNS IN THE SPATIAL STRUCTURE OF PRAGUE'S METROPOLITAN REGION IN 1996 - 2006
Škorpil, Jan ; Novák, Jakub (advisor) ; Kostelecký, Tomáš (referee)
This thesis deals with geographic analysis of political behaviour. It is spatially limited to a metropolitan area of Prague, the capital and biggest city in the Czech Republic. Political behaviour of voters is analysed on election results of parlamentary elections between 1996 and 2006. The analysis uses agragate data and is undertaken in three main different sets of spatial units. Rusults are then compared inbetween them to confirm or refute obtained findings. The main goal of this thesis is to discover and analyse existing spatial patterns of political support for individual parties. As the czech political system is based on proportional representation, five main political parties and parties groupings are analysed. The second goal is to capture the change in political behaviour over the sudied time period. To discover how high the spatial stability of different party support is and which parts of the metropolitan region has undergone the biggest change in political behaviour. Last goal is to try to explain what changes has the process of resididential suburbanization done to the existing spatial structure of party support. The main conclusion is that the existing pattern of political party support has been on the level of main morphological zones reversed. The main right-wing party of the czech...
|
|
The Role of WT1 gene in normal hematopoiesis and leukemogenesis
Kramarzová, Karolina ; Trka, Jan (advisor) ; Zadražil, Stanislav (referee)
6 Abstract The Wilms' tumor gene is highly expressed in a large proportion of human acute leukemias and other hematological malignancies. It is thought to play an important role in human hematpoiesis and leukemogenesis. Despite the large number of studies, WT1 expression patterns and its clinical significance in acute myeloid leukemia remain still controversial. To investigate the prognostic relevance of initial WT1 expression and its usefulness as a marker for minimal residual disease, we have analysed 66 bone marrow samples from newly diagnosed AML patients. RQ-RT-PCR for absolute quantification of total WT1 was designed according to Europe Against Cancer Program. In 82 % of samples we detected a higher expression of WT1 compared to normal healthy donors or patients with acute lymphoblastic leukemia (p<0.0001). We did not find any correlation between initial WT1 expression level and age or sex. Patients with FAB M3 subtype showed significantly higher WT1 levels than other subtypes, especially M5 (p<0.001). We found a strikingly high WT1 expression in standard risk group patients compared to high risk group (p<0.0007). Children with FAB/cytogenetical favorable prognostic factors have high WT1 expression, while there was no relation between WT1 levels at diagnosis and day 15 BM response. We did not find any...
|
|
Optimization of capital structure and shareholder's investment return
Kudela, Petr ; Marinič, Pavel (advisor) ; Genža, Jiří (referee)
Management of most of the companies pays substantially less attention to structure of raising capital, than to investing it. Sometimes managers forget that the value of the company can be increased not only through proper investments but also through choosing the right proportion of capital structure. There are many theories dealing with this issue. The best known, Miller-Modigliani theory considers as an optimal capital structure the one with the lowest weighted average cost of capital. This thesis is based on these theories and tries to apply them on Czech firm and its Brazilian daughter company.
|
| |
guest ::
