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Analysis and design of data services for customers on the basis of available data sources in the enterprise. (Vodafone company)
Brdjar, Jaroslav ; Pour, Jan (advisor) ; Machander, Aleš Machander (referee)
This thesis is concerned with analysis of the mobile operators in the Czech Republic. Introduction part of thesis is devoted to the explanation of the key indicators which are used by mobile operators to analyze the rate of customer churn, or average revenue per user, or the customer value. Very importat is also a comparison of price consumption baskets in the Czech Republic with the other countries. The situation has changed dramatically offering unlimited tariffs in the already fully saturated market. In the practical part of this thesis I have focused on a detailed analysis of customers data in Vodafone company a I have reviewed the current offer of affordable tariffs and data services. I tried to implement new tariffs based on a real information of all customers - individuals over a period of 3 months. The aim of the thesis is to propose tariffs and services for customers, which would maintain the current customer base or which would increase the base slightly.
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Vliv silážních inokulantů na bachorovou degradovatelnost škrobu silážovaného kukuřičného zrna
Valentová, Miroslava
Ruminal degradability of starch is an important indicator of utilization of starch by rumen microflora. Indicates the ratio between starch, which is fermented in the rumen, and starch, that escapes ruminal digestion. Determination of rumen degradability of starch is important not only in terms of performance and health of dairy cows, but also in terms of economy of farming. Corn starch, compared to other starch rom cereals, has lower rumen degradability, which is a prerequisite for efficient use of animals. The aim of this thesis was to evaluate the effect of silage inoculants on the quality of the fermentation process, the chemical composition of silage and level of rumen degradability of starch in model silage from mechanically treated corn grain. Ruminal starch degradability was determined by in sacco method. Treatment of matter by silage additives had statistically significant effect neither on the chemical composition, nor on ruminal degradability of starch. In the evaluation of the fermentation process was at application of silage inoculant found statistically significant effect (P <0,05) on dry matter content , pH , content of ammonia , acetic acid , the ratio of lactic acid and acetic acid and ethanol. For silage treated with inoculant was statistically significant higher solids content ( 695,36 +- 2,342 g/kg) and a statistically significant lower pH ( 3,99 +- 0,002) compared to control. Neither in acidity of water extract nor in the content of preserving lactic acid, was found statistically significant difference. For the inoculated silage was found statistically significantly lower content of acetic acid (4,10 +- 0,157 g/kg DM). The content of propionic acid and undesirable butyric acid was for any silage found. In overall content of fermentation acids was no statistically significant difference. The ammonia content, which represents the decomposition of nitrogenous substances, was in the inoculated silage statistically significantly lower (0,53 +- 0,031 g/kg DM) than in untreated silages and so was statistically significantly lower the content of ethanol (0,94 +- 0,145 g/kg DM). The use of silage inoculant had no statistically significant effect on the acidity of the water extract, lactic acid content and the amount of acids.
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Risks of the Road Transport And Their Factors
HEJKRLÍK, Lukáš
The bachelor thesis Risks of the Road Transport And Their Factors deals mainly with the risk of avenues that are planted along the Czech roads. I have focused on basic definitions that are closely connected to road transport and I have also focused on ex-planation of practical methods used when assessing traffic accidents in the theoretical part of the thesis. In the practical part of the thesis I have focused on concrete analyses related to existence of avenues along the Czech roads. These analyses confirm the de-gree of risk of road transport itself but especially fatal consequences of accidents caused by a crash with a fixed obstacle that is often presented by a tree from avenues along the roads. The analyses discovered that the accident rate in the Czech Republic is decreasing. However, the fact that the risks connected with consequences of tree crash are decreas-ing more slowly, is still alarming. The detailed graphs and tables in the bachelor thesis show the number of casualties connected to a traffic accident caused by a crash with a fixed obstacle and they prove that the consequences of accidents caused by a crash with a tree are worse than the consequences of accidents caused by a crash with a crash bar-rier, even though they both are considered a fixed obstacle. Last but not least the costs and total economic losses caused by the highest negative factor, i.e. the accident rate, are calculated by using the tables and graphs.
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Populačně genetická struktura pstruha obecného jako základ úspěšného obhospodařování lososových vod ve střední Evropě
KOHOUT, Jan
The genetic structure of 25 wild populations and five hatchery stocks from Czech Republic and Slovakia were analysed using mitochondrial (control region) and nuclear DNA (microsatellites, LDH-C1*) markers to elucidate the impact of stocking on central European populations of brown trout and to outline further management strategies. It seems that stocking practices have caused massive hybridisation between the Atlantic and Danube brown trout populations in the middle Danube basin and have led to a loss of among-population genetic variability in Slovakia and Moravia. Certain effect of stocking was detected also in the upper Danube, Vistula, Oder and Elbe River basins. However, the populations from the Elbe River basin keep certain level of among-population variability and seem to be less affected by stocking in comparison with the Danube River basin populations. There are some indications of late or post-Pleistocene penetration of the Atlantic basin trout to the Danube River basin. However, it is not clear to which extent the natural contact participated to the present distribution of Atlantic haplotypes and alleles in the Danube River basin. Samples from lower parts of the Danube River basin were therefore analysed using the same mitochondrial and microsatellite markers. Samples from Aegean Sea basin were included in order to reveal genetic variability of eastern Balkan populations and to estimate an impact of stocking in this area. Very low levels of introgression from Atlantic and other non-indigenous trout were found in the eastern Balkan populations. The genetic differentiation among the populations is substantially higher in this area compared to the central European populations. The populations in headwaters of the Otava River (Elbe River basin) were analysed using microsatellites in order to reveal origin of these populations and evaluate the current management strategies of brown trout in Šumava National Park and Protected Landscape Area. The analysed populations were substantially differentiated from the remaining Elbe River basin populations and there was also certain level of genetic structure within trout from the headwaters of the Otava River associated with isolation by a migration barrier and geographic distance. However, stocking with hatchery trout also contributed to the pattern of genetic variability. The population of Borová Lada hatchery, which is used for stocking in Šumava exhibited higher genetic variability compared to the wild populations and it seems to be of heterogeneous origin. Comparisons of the analysed populations with populations from other areas and results from other studies indicated that mtDNA haplotypes from the lower Danube River and southern Black Sea basins differ considerably from a subclade of the Danubian lineage consisting of haplotypes found so far in the most of the Danube River basin and in the Caspian and Aral Sea basins. The results thus evidence a complex evolutionary history of brown trout in the southern and western parts of the Black Sea basin.
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Benefits of quantitative sputum processing in diagnosis of respiratory tract infections
KOUBOVÁ, Lucie
Lower tract respiratory infections (LRTI) remains worldwide among one of the most common causes of morbidity and therefore the early diagnosis and timely onset of effective antibiotic therapy is very important. The most often non-invasive material from LRT sent for microbiological analysis in suspected infections of LRT is spontaneously expectorated sputum. Basic method for its processing is then microscopy and cultivation analysis. For laboratory and consequently for the attending doctor to obtain the most exact correlation between the laboratory interpretation of the result and the clinical status of the patient is to asure clinical as well as technical validity of the sample. The purpose of this work is the comparison of two bacteriological methods for processing the sputum samples ? classic cultivation method of undiluted sputum samples and quantitative sputum culture. Another aim is to evaluate whether the quantitative sputum culture when only purulent samples are cultivated allows microbiologist higher quality interpretation of the analysis result. Clinical samples of spontaneously expectorated sputum were processed at the Department of Clinical Microbiology and Antibiotic Station (KMAS), Central Laboratories, Strakonice Hospital from April to October 2012. During the six months period KMAS received in total 266 samples of sputum, out of which 230 came from the hospitalized patients and 36 came from primary and outpatients. Sputum samples were received from the patients suspected of LTRI and in most of the cases sputum was collected prior to antibiotic therapy.All the samples were analysed by both compared methods. In classic cultivation method simultaneously with the microscopic Gram analysis the sample was inoculated on selected culture media. During the quantitative sputum culture the microscopic Gram analysis was performed first and contaminated sputa coming from URT were excluded from further analysis. Quantitative sputum culture was then performed only on purulent sputum samples with low rate of squamous epitelial cells. Those were homogenised prior the cultivation itself. The results of both methods were compared with the clinical status of the patient and then clinical characteristics of the methods were statistically analysed. They allowed the assessement of the precision of a particular method. All 36 samples from outpatients and primary care patients were processedby classic cultivation method. Sensitivity of classic cultivaiton method versus quantitative culture method reached 100%/ 100%, respectively, specificity reached 69%/74%, efectivity 69%/75%, positive predictive value 3%/3%, negative predictive value 100%/100% and positive credibility of laboratory analysis equaled 3,18/ 3,89. Out of the total 266 recieved samples from hospitalized patients 130 samples (57%) were processed by quantitative sputum culture on the basis of microscopic Gram analysis. Sensitivity of classic cultivation method versus quantitative culture method for these samples reached 62%/ 72%,, respectively, specificity reached 54%/67%, efectivity 56%/68%, positive predictive value 12%/14%, negative predictive value 54%/67% and positive credibility of laboratory analysis equaled 1,13/ 2,16. On the basis of the obtained results it is possible to recommend the quantitative sputum culture as the method which improves the interpretaion in the diagnostic of LRTI in all hospitalized, outpatient and primary patients. By excluding the material which is not suitable for quantitative sputum culture is not only shortened the time for the result interpretation but also consumer material is safed in the laboratory. The sputum samples cannot be excluded exclusively on the basis of microscopy analysis. Diagnosis of the patient must to be taken into account as well. Quantitaive sputum culture in microscopically valid samples allows microbiologist predict with greater accuracy colonising suspect pathogens and isolates truly pathogenic.
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Current state of skialpinism
Formánková, Dita ; Baláš, Jiří (referee) ; Vomáčko, Ladislav (advisor)
Title: The current state of racing ski mountaineering Aim: Goal of this thesis is to collect current information on ski mountaineering race, physiological characteristics of selected skialpinists, document pulse load skialpinists at selected sites and to prepare a thesis. method: The analysis of texts, field and laboratory testing, monitoring longitudiální skialpinists. results: The system of competition is currently at the World Championships, European Championships, World Cup and others. In the Czech Republic held a series of Czech and Central European Cup. Races are held in the form of "start-finish", vertical, couples, and relay Ralley. All tested individuals reach high to very high preparedness the measured values (VO2max, etc.) The value of percent body fat is responsible for all values reported by individuals for sporting population. Heart load measured at sites correspond to the type of race and all the circumstances. Keywords: Ski touring, ski mountaineering competitions, heart rate (load), aerobic load anaerobic load, maximum oxygen consumption (VO2max)
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Cytokine estimation in liver homogenate supernatant in rats with liver steatosis
Burešová Nedvídková, Jaroslava ; Živný, Pavel (referee) ; Dršata, Jaroslav (advisor)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Jaroslava Burešová Nedvídková Supervisor: Doc. MUDr. Pavel Živný, CSc. Title of diploma thesis: Cytokine estimation in liver homogenate supernatant in rats with liver steatosis The aim of this work was the determination of cytokines - interleukin 6, tumor necrosis factor-alpha and leptin levels in liver homogenate supernatant in rats with hepatic steatosis. These tests were performed within extensive experimental work intented on the ability of liver regeneration after induction of steatosis. The determination was performed using enzyme-linked immunosorbent assay. The results were compared with cytokine concentrations of Wistar and PHHP control groups, who were fed a standard laboratory diet. The concentrations of IL-6, leptin and TNF-alpha in liver tissue supernatant were higher in the control group of Wistar rats than in PHHP rats control group. Receiving steatosis diets led to a decrease in IL-6 for all rat files. Leptin concentrations were significant lower in Wistar rats than Wistar rats controls were. Orotic acid administration statistically significant increased leptin level in rats PHHP. The consumption of diets causing steatosis reduced TNF-alpha concentrations in liver...
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The study of influence inositolhexaphosphate on proliferation and apoptosis in cells isolated out of colorectal cancer
Hašková, Pavlína ; Šimůnek, Tomáš (advisor) ; Rudolf, Emil (referee)
Introduction: Apoptosis - programmed cell death significantly participates in maintaining of tissue homeostasis. Its alteration is leading to cancer disease. Inositolhexaphosphate (IP6) is naturally occurring substance that is present in most legumes, cereals and seems. IP6 and its lower phosphorylated forms are also found in most mammalian cells, where they assist in regulatory a variety of important cellular function (including regulation of cell cycle). Methods: This work has been engaged in the study of influence IP6 on apoptosis in cells isolated out of colorectal cancer by in vitro quantification enzymatic activity of caspase 3 in cell extract. Results: It has proved by the Student's t-test that IP6 statistically significant influence apoptosis in cells isolated out of colorectal cancer.
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Francisella Tularensis infection of macorphage-like cell line J774.2 - changes of surface and intracellular molecules
Novosad, Jakub ; Krčmová, Irena (advisor) ; Raška, Milan (referee) ; Kročová, Zuzana (referee)
Summary: Background: Francisella tularensis (F.t.) is a facultative intracellular bacteria, enrolled at the list of Centre for Disease Control (CDC) as a high risk bioterrorism agent, category A. There is a long-term effort to understand to the immunopathogenesis of F.t. infection. The aim of our study was focused on phenotype analysis (CD54, CD16/32 and CD86) and nitric oxide (NO) production analysis of murine macrophage-like cell line J774.2 durig F.t. live vaccine strain (LVS) in vitro infection. J774.2 cells were either untreated or stimulated either before or after F.t. infection by interferon gamma (IFNγ), lipopolysaccharide (LPS) separately or in its combination. Method: We followed up the expression of cell surface markers and NO production 3, 6, 9, 12 and 24 hours after initiation of infection with or without stimulation using flow cytometry and Griess method respectively. The expression was followed as either absolute value of mean fluorescence index (MFI) or as relative change of MFI (∆MFI). Murine macrophage-like cells (J774.2) were incubated in cultivation flasks (2x106 cells/10ml of medium Dulbecco's MEM with Glutamax-1 with 10% BSA). The cells were activated with 10 or 50 ng of LPS / 1ml of medium or with 100 or 1 000 I.U. of IFNγ / 1ml of medium separately or in combination (10 ng/ml and 100...
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Possibilities of the application of supplemental and nonconventional methods in prevention and health care in calves
KONRÁDOVÁ, Petra
Diarrheal diseases of calves are one of the most serious risks of rearing calves and cause economic losses. For this reason, the objective of this work was to assess the influence of means of support in operating conditions for the incidence of diarrheal disease in calves in the early post-weaning stages. A company was chosen for this work in which both the company employees and an external veterinarian were willing to collaborate and provide not only information about the breed in question, but also biological material. This company was JINOS - AGRO Veselí nad Lužnicí. The calves were observed in the period of April - June 2009. The calves were housed in separate boxes the second day after birth until the average age of 56 days. After storage, they were divided into 5 groups: 4 based on the chosen means of support, and a single control group. Faeces samples were collected from the calves and subsequently evaluated. The calves to whom were administered means of support were then evaluated to assess the effect thereof on the incidence of diarrhea, the duration of diarrhea, the method of treatment, and the duration of treatment. The results were summarized and analyzed using a statistical program (STATISTICA 7.0.). There was no statistically significant difference found between the individual groups and the experimental group. An evident and statistically significant difference was found only in the case of a difference between the drugs of the 2 experimental groups, specifically between Lactovita and Homeopatika. Based on this observation, it can be stated that there was no evidence supporting the effectiveness of administered means of support for the incidence of diarrheal disease in calves in the early post-weaning stages.
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