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GSM-EDGE and UMTS Systems Coexistence
Gleissner, Filip ; Hanus, Stanislav (advisor)
The dissertation thesis deals with the investigation of the coexistence of GSM–EDGE and UMTS systems with focus on the physical layer of the systems. The aim is to provide a set of recommendations for practical cooperation while the systems operate in both the separated and the common radio bands. A detailed description of signal processing of both the systems on the physical layer is presented. On the basis of this description, models of both systems were created and implemented in the MATLAB environment. The simulations are focused on the physical layer quality parameters, especially the bit error ratio evaluation for various ratios of useful signal power to noise power during the transmission over the radio channel. Simulation is also used to examine the quality of received useful signal while it is interfered by signals from the same and adjacent channels. The purpose is to determine the isolation between these adjacent channels, when the bit error ratio of the useful signal does not exceed a certain reference value. The simulation results are subsequently subjected to comparison with the results of the experimental measurements in laboratory conditions. Before the measurements are carried out, a study of possible interference types is performed. Consequently, the crucial parameters of the measuring equipment used are verified. From the results of simulations and measurements, the proposal of a minimum and recommended carrier separation between both the systems is presented in order to efficiently utilize the assigned frequency spectrum. Furthermore, for the cooperation in both the separated and the common radio bands, the necessary precautions are given for reaching the required isolation and thereby inter-system interference minimization.
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A Low Voltage Wiring System for an Office Block
Nestrojil, Martin ; Slavata, Petr (referee) ; Kadlec, Radim (advisor)
This paper describes the design of a low voltage network in an office building. In the beginning work is given for the theoretical part of the topics that we need to create a network design in a given building. In this part of the guiding principles are conductor sizing, fuse wires and a selection of protective devices. Everything was sought in scientific publications and Articles. The theoretical part will be followed by a practical part with the first adjusted underlying schema, and then will address a practical design using programs supplied by the company Eaton. The goal of this work is to get acquainted theoretically with the main principles of network design, and then practically with your programs and create a complete network design of the building.
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Molecularly imprinted polymers as recognition elements for the determination of disease markers
Vodová, Milada ; Vaculovičová,, Markéta (referee) ; Bezděková,, Jaroslava (advisor)
The diploma thesis is focused on the preparation and optimization of molecularly imprinted polymers (MIP) selective for chymotrypsinogen A as well as on the use of these MIP as recognition entities in the sensor. MIP was prepared by suspension polymerization using a mixture of functional methacrylate-based monomers. Prepared MIP was optimized (e.g. binding properties, selectivity and isolation of chymotrypsinogen from a complex matrix of human breath) by capillary electrophoresis with fluorescence detection ( = 532 nm). Finally, the combination of MIP with quartz microbalances was demonstrated as a promising sensor for the detection of proteins from human breath condensate.
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Molecularly imprinted polymers as a tool for the isolation of key biologically active molecules
Vodová, Milada ; Jaroslava, Bezděková (referee) ; Nejdl, Lukáš (advisor)
This bachelor thesis is focused on preparation and optimization of molecularly imprinted polymers that are selective for the selected nucleobase(uracil). Molecularly imprinted polymers, which wereused for selective uracil isolation, have been prepared non-covalent imprinting technique. As a function monomer was used in this case dopamine. The detection ofisolated uracil was made by capillary electrophoresis with absorption detection (–260 nm). Conditions for preparation of molecularly imprinted polymers,their binding properties, adsorption kinetics, and selectivity wereinvestigatedin detail. Mentioned polymer materials were as well used for uracil selective isolation and detection from complexsamples. Tomato products were used as complex samples. Uracil in those samples was usedasanindicator ofbacterial contamination.
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The silicon etching technology
Krátký, Stanislav ; Ježek,, Jan (referee) ; Matějka, Milan (advisor)
This thesis deals with the silicon etching technology. It Examines using of water solution of potassium hydroxide. It focuses on plasma etching of silicon using mixture of CF4 and O2 as the dry way of etching. Important parameters of etching like etching rate of silicon and masking materials, etching selectivity, surface roughness and underetching of mask are determined for both ways. Some additional processes has been examined as well, namely creating of mask of resist and silicon dioxide, lithography process and etching of resist using oxygen plasma.
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Protection of Transformer by Differential Relay
Jára, Jaroslav ; Haluzík, Evžen (referee) ; Toman, Petr (advisor)
This bachelor’s thesis deals with transformer failures and principles of their protection and a design of differential protection of transformers. The first part details with transformer failures, classification of these failures, short-circuit ground faults types, causes of the short-circuit occurrences and consequences of oil-transformer failures. The second part deals with problems of protection in general with the description of protection function, requirements of protection such as selectivity, speed of response, sensitivity and protection classification, methods of protection according to failure type - size of nominal power of transformers. It further describes principle of differential protections. Finally there are previews of historical and modern protections. The third part describes a design of differential protection of transformers. It includes short-circuit ratio calculation, example of three phase short-circuit calculation, CT ratio check, actual setting of protection and setting evaluation.
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Reactive modifications of RNA for bioconjugations with proteins and new enzymatic methods for the synthesis of base-modified RNA
Brunderová, Mária ; Hocek, Michal (advisor) ; Míšek, Jiří (referee) ; Vaňáčová, Štěpánka (referee)
This doctoral thesis focuses on the enzymatic synthesis of base-modified RNA probes with diverse functional groups, including reactive cross-linking, hydrophobic and fluorescent moieties, or affinity tags. The construction of nucleobase-modified oligonucleotides is accomplished either through conventional in vitro transcription with T7 RNA polymerase or by an innovative approach leveraging engineered mutant DNA polymerases and primer extension reaction (PEX). In the first section of the thesis, a novel ribonucleoside triphosphate building block with reactive chloroacetamide functionality was synthesised using an aqueous Pd-catalysed Sonogashira cross- coupling reaction, directly applied on iodinated nucleotide. The chloroacetamide modified triphosphate was then tested as a putative substrate for T7 RNA polymerase in in vitro transcription reaction, aiming to construct RNA probes with one or multiple reactive groups. The selectivity of chloroacetamide- modified RNA for thiol-, or cysteine-, and histidine-containing (bio)molecules was demonstrated by model bioconjugation reactions and cross-linking experiments with three RNA-binding proteins of diverse structures and functions. The efficient formation of RNA-protein covalent adducts was confirmed by western blot or gel, and mass spectrometry analyses...
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Evaluation of the properties of new types of zwitterionic stationary phases in HILIC
Nováková, Daniela ; Nováková, Lucie (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Daniela Nováková Supervisor: prof. PharmDr. Lucie Nováková, Ph.D. Title of the diploma thesis: Evaluation of properties of new types of zwitterionic stationary phases in HILIC The aim of this thesis was to compare six new zwitterionic Astra ZWIX columns (3 μm, 2,1 x 100 mm) from Chromservis s.r.o. Five groups of compounds were used for the analysis, namely nucleic bases and nucleosides, nicotinic acid derivates, hydrophilic vitamins, beta- blockers, and phenolic acids. These groups of compounds were also analysed on commercially available columns - BEH Z HILIC, SYNCRONIS, OMEGA SUGAR, BEH HILIC and BEH AMIDE (OMEGA SUGAR 3 μm, 2,1 x 100 mm; other columns 1,7 μm, 2,1 x 100 mm). The investigated parameters were retention time, peak resolution, peak symmetry, and peak width at half height. Optimisation to improve peak separation of nucleic bases and nucleosides, hydrophilic vitamins, and beta-blockers was also carried out. The mobile phase gradient was optimized, as well as the column temperature, and mobile phase flow rate. The selectivity of the columns was found not to differ within the batch. The retention time repeatability condition was met for all analytes on all columns, except for...
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Pastva oviec v Národnej prírodnej rezervácii Mohelenská hadcová step
Sečkárová, Tereza
The diploma thesis deals with the impact of sheep grazing on the protected steppe biotopes of the National Nature Reserve Mohelno Serpentine Steppe. It focuses on the ethological manifestations of the sheep flock and on the evaluation of the state of the pasture before and after the end of animal grazing in enclosures 7 and 8. Based on the results of the ethological observations, it is likely that the grazing activity of the sheep was influenced by the outside temperature, while in most cases it was increased at lower temperature. It can also be assumed that the sheep continued their grazing activity even after the end of the ethological observation. There was no significant difference in the height of the grasses before and after the end of grazing in the given fences, but the selectivity of the sheep regarding herbs and lady´s bedstraw (Galium verum), which was abundantly represented on the pasture, was confirmed. The results are processed into tables and graphs, and photographic documentation can be seen in the appendices of the work.
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Advanced extraction materials for sample preparation in chromatographic analysis
Zatrochová, Slavomíra ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee) ; Ševčík, Juraj (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Pharmaceutical Analysis Candidate Mgr. Slavomíra Zatrochová Supervisor Prof. RNDr. Dalibor Šatínský, Ph.D. Advisor PharmDr. Ivona Lhotská, Ph.D. Title of Doctoral Thesis Advanced extraction materials for sample preparation in chromatographic analysis The submitted dissertation presents an annotated set of publications focusing on advanced extraction materials in analytical chemistry and their prospective use as sorbents in sample preparation prior to HPLC analysis. The work was created based on the cooperation with the Technical University of Liberec and the University of Valencia in Spain. In the first part, a new sorbent based on a hybrid monolith with a metal-organic framework (MOF) was developed for conventional Teflon-coated magnetic stir bars, and the resulting hybrid monolith was evaluated as a stir bar sorptive extraction (SBSE) sorbent for the extraction of estrogens in water and urine samples. In the second part, the work was focused on nanofibrous and microfibrous polymers and the production of fibers by alternating current (AC) electrospinning. The extraction efficiency, selectivity and stability of different polymer nanofibers were tested. The...
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