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Methods for class prediction with high-dimensional gene expression data
Šilhavá, Jana ; Matula, Petr (referee) ; Železný, Filip (referee) ; Smrž, Pavel (advisor)
Dizertační práce se zabývá predikcí vysokodimenzionálních dat genových expresí. Množství dostupných genomických dat významně vzrostlo v průběhu posledního desetiletí. Kombinování dat genových expresí s dalšími daty nachází uplatnění v mnoha oblastech. Například v klinickém řízení rakoviny (clinical cancer management) může přispět k přesnějšímu určení prognózy nemocí. Hlavní část této dizertační práce je zaměřena na kombinování dat genových expresí a klinických dat. Používáme logistické regresní modely vytvořené prostřednictvím různých regularizačních technik. Generalizované lineární modely umožňují kombinování modelů s různou strukturou dat. V dizertační práci je ukázáno, že kombinování modelu dat genových expresí a klinických dat může vést ke zpřesnění výsledku predikce oproti vytvoření modelu pouze z dat genových expresí nebo klinických dat. Navrhované postupy přitom nejsou výpočetně náročné.  Testování je provedeno nejprve se simulovanými datovými sadami v různých nastaveních a následně s~reálnými srovnávacími daty. Také se zde zabýváme určením přídavné hodnoty microarray dat. Dizertační práce obsahuje porovnání příznaků vybraných pomocí klasifikátoru genových expresí na pěti různých sadách dat týkajících se rakoviny prsu. Navrhujeme také postup výběru příznaků, který kombinuje data genových expresí a znalosti z genových ontologií.

Comparison of the performance of economic entities
LUKŠ, Mirek
The diploma thesis deals with a construction of the appropriate comparative model on the methodical basis of multi-criteria evaluation of alternatives, which are based on the economic indicator considered as a standard for determination the specific order of the companies in this thesis compared. Moreover, there is further a comparison incorporated, which consists of the position of in the thesis selected comparative model against regularly existing models such as specifically the Altman index (Z-score) and the index IN99. An integral part of the thesis is also a graphical analysis of the specific the model entering inputs such as indicators, criterions, which are considered to be a crucial part for the correct utilization of the model.

Iniciace pohybu byčíku, signalizace a regulace pohyblivosti spermií ryb: fyzikální a biochemické řízení
PROKOPCHUK, Galina
The current study attempted to shed light on the regulatory processes and response arrangements of fish spermatozoa during the course of maturation and motility initiation. The first intent of this study was to improve the understanding of the mechanism underlying the acquisition of potential for sperm motility in sturgeon. Up to present work, the physiological process underlying sperm maturation in this species has not been described at all. Our results showed that sperm maturation in sturgeon occurs outside the testes because of dilution of sperm by urine and involves the participation of high molecular weight substances as well as calcium ions present in seminal fluid and/or urine. The second aim of the present study was to investigate the coping mechanisms in fish spermatozoa with osmotic and ionic activating mode, as well as in spermatozoa of euryhaline fishes, to various environmental conditions. We showed that alteration of environmental osmolality might affect the fish sperm in different ways, depending on fish species and modes of spermatozoa motility activation either osmotic or ionic mode. In response to osmotic stress caused by hypotonicity, carp spermatozoa regulated the flow of water across their cell membrane and increased their cytoplasmic volume during their short motility period. In contrast, no indications of sperm volume changes were observed neither in sterlet nor in brook trout spermatozoa, both of which having an ionic mode of motility activation. We also examined the mechanism by which sperm motility triggering in euryhaline fishes can adapt to a broad range of environmental salinity. Our results demonstrated that spermatozoa of euryhaline tilapia, Sarotherodon melanotheron heudelotii, reared in fresh-, sea- or hypersaline water can be activated in hypotonic, isotonic or hypertonic conditions of swimming milieu, provided Ca2+ ions are present at various levels. It was established that the higher the fish rearing salinity or the more hypertonic ambient media at spermatozoa activation, the higher extracellular concentration of Ca2+ ions is required. The results obtained in the present study allow suggesting that osmolality is not the main factor inhibiting sperm motility inside the testis in the S. melanotheron heudelotii. A third aim of this study was investigation of the regulation of motility initiation process and description of flagellar beating initiation in chondrostean spermatozoa. We detected that K+ inhibition of sperm motility in sturgeon can be by-passed due to the pre-exposure of sperm cells to a high osmolality shock prior to its transfer to K+-rich swimming media. Thus, we hypothesized that sturgeon spermatozoa may be activated by use of an unexpected signaling pathway, independent from regular ionic stimulation. The successive activation steps in sturgeon spermatozoa were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds. At motility initiation, the first couple of bends formed at the basal region begins to propagate towards the flagellar tip, but gradually fades when reaching the mid-flagellum. This behavior repeats several times until a stage where the amplitudes of bends gradually reach similar value, what eventually leads to sperm progressive displacement. The total period needed for the flagellum to switch from immobility with rigid shape to full activity with regular propagating bends ranges from 0.4 to 1.2 seconds. In conclusion, the results of the current study bring valuable pieces of information into the general understanding of the processes of maturation of fish spermatozoa, their adaptability to different physical and biochemical circumstances, the extra- and intra-cellular signaling as well as the regulatory mechanisms of motility activation in fish spermatozoa.

Analysis and modeling of the structure and development of mixed forest stands in the Sudety mountains
Vacek, Zdeněk ; Remeš, Jiří (advisor) ; Hlásny, Tomáš (referee)
The thesis deals with analysis and modeling of the structure and development of selected mixed forests in protected areas of the Sudeten system, especially in the Giant Mountains national parks, Protected Landscape Area Broumovsko and Orlické Mountains, but also in other areas of the Czech Republic. This study is composed of a set of six published manuscripts that are covering three thematic ranges: structure of forest stands, regeneration of forest stands and forest modeling. The main objective of this work was to evaluate a vertical, horizontal and species structure, total diversity and development of mixed forest stands in central Sudetes. The partial aim was to analyse production parameters of forest stands, effect of microrelief and game on natural regeneration and assessment of dead wood in the area of interest. Further, the objective of the study was to develop explicit and non-explicit crown width and slenderness quotient models for Norway spruce (Picea abies L.) and European beech (Fagus sylvatica L.) and to predict the development of mixed forest ecosystem using growth simulations and to evaluate parameters and interactions among stand structure, climatic factors and natural regeneration, using especially analysis of variance, correlation matrix, spatial statistic and multivariate analysis. For this purpose a system of permanent research plots was used, which are regularly monitored since 1980 or were newly established. Using mapping technology FieldMap, selected parameters were measured for tree layer, natural regeneration individuals and dead wood. The results showed that the spatial distribution of beech stands in optimum stadium changes with the altitude from the regular pattern through random to aggregated spatial pattern of beech forests near the timberline. The spatial distribution of natural regeneration is highly aggregated, distribution of stumps is random and horizontal structure of the centroids of the crowns is always more regularly distributed than stems due to crown plasticity. Browsing damage of the leading shoot by game is an important limiting factor for height growth of natural regeneration, especially for silver fir (Abies alba Mill.), rowan (Sorbus aucuparia L.) and sycamore maple (Acer pseudoplatanus L.). The study of the influence of microrelief on the growth of beech regeneration showed that the highest average height was found on slope and pits, while the lowest on the mounds. From the effect of climatic factors on the radial growth of trees, it was found that temperature is a limiting factor for growth in mountain areas, respectively that positive effect of temperature decreases with decreasing altitude and conversely the influence of precipitation increases. Finally, spatially explicit models (as opposed to non-explicit) described a larger part of the crown width variations for spruce and beech and of the slenderness quotient for spruce. The largest contribution to the models after breast diameter was dominant height.

Construction and scientific implementation of mathematical models for tree compartments of broadleaved trees in growth stages of seedlings and young stand
Pajtík, Jozef ; Konopka, Bohdan (advisor) ; Monika, Monika (referee)
Importance of precise estimation for tree biomass in forests has been continuously increasing. Regarding to the climate change, scientists have started to quantify all tree components not only in terms of energetic utilization but also for carbon stock estimation. Increasing relevance of biomass models for young trees relates to expanding area of young forest stands during the last period due to decay of old forests often caused by disturbances (especially: windstorms, outbreaks of bark beetles, drought episodes, and forest fires). Models for biomass stock estimations constructed for stands with age to 10 years are rare and usually are focused on aboveground tree parts. Thus, this work aims at filling knowledge gaps in this field. Its main objectives are: 1) construction of regression models applicable for estimation of dry mass in the particular tree components (i.e. stem, branches, foliage, roots) for young stands of some broadleaved species, 2) implementation of regression models for calculation of biomass conversion and expansion factors (BCEF), allocation coefficient, growth efficiency and leaf area index (LAI) and their inter-specific comparison, 3) utilisation of allometric relations for estimation on forage potential for ruminating ungulate game (i.e. browsing and stripping). To make up the models, destructive tree sampling will be implemented. The sample trees will be excavated, separated into tree components, dried for constant weight and weighed. Log-transformed relationships will be used for construction of regression models.

Vertical ground exchangers as a source of energy
Mašek, Lukáš ; Neuberger, Pavel (advisor) ; Daniel, Daniel (referee)
The dissertation on the topic of "vertical ground heat exchangers as a source of energy" deals with boreholes as a source of energy for the heat pump. In the chapter "The current state" is gradually described problems geothermal energy, open and closed systems, the distribution of thermal boreholes by construction and at the end of the chapter focuses on the thermal test wells. In the chapter "Methodology", in the theoretical part are zoomed simplistic relationships that describe the heat conduction around the borehole, analytical models, numerical models and a combination of numerical and analytical models that are able to describe two or three-dimensional conditions. In the experimental part of this chapter is given space characteristics of the place of measurement and definition of terms attenuation depth and heat transfer coefficient. The final chapter of the dissertation "Results and discussion" presents concrete results.

Using Weather Generators for the Assessment of the Impact of Climate Change in Catchments
Martínková, Marta ; Hanel, Martin (advisor) ; Máca, Petr (referee)
The main objective of this dissertation is to provide a novel approach to downscaling of outputs from regional climate models and to simulation of future climate. The resulting method consists of rain generator that operates in 6-hour time step. The generator performs well for the observational data. It consists of following steps: disaggregation of 6-hour cumulative precipitation into convective and stratiform types, fitting of first order 3-state discrete time Markov chain to the data and simulation of long time series of precipitation. Then the mixture of log-normal and Generalized Pareto distribution is fitted to stratiform events and the Generalized extreme value distribution is fitted to convective events. The impact of climate change on precipitation is evaluated by using change factors that are identified for precipitation occurrence (by comparing the transition matrices for the future and control period) and for precipitation amount (by comparing the scale and location parameters of distributions fitted for the future and control period). The observational data are then altered with obtained change factors. From evaluation of observational data it stems that the average volume of an convective event is higher for the western region than for eastern region of the Czech Republic. Additionally, statistically significant trends in number and volume of convective events were identified for the region. The relative portion of convective precipitation is the highest in summer for observational data. From analysis of RCMs simulations, it stems that even though the overall precipitation is projected to be lower in future, the proportion of convective events (versus stratiform ones) would be higher. The number of convective events is projected to be lower in the future, while the volume of a convective event to be bigger.

Statistical correction of daily precipitation sums from climate models
Hnilica, Jan ; Chára, Zdeněk (advisor) ; Jan, Jan (referee)
Climate change prediction and evaluation of its impact currently represent one of the key challenges for the science community. Regional climate models (RCM) have been recently established as a main source of the data for climate change assessment studies. Nevertheless, RCM outputs suffer from systematic errors caused primarily by their low spatial resolution and cannot be used directly without any form of bias correction. The bias correction is an actual topic in climatology and several correction methods were developed, ranging from the simple additive method to more advanced approaches (e.g. quantile mapping). However, despite this progress, the bias correction methods suffer from several difficulties, which bring another source of uncertainty into the climate change impact assessment studies. This thesis is focused on two problematic points connected with the bias correction of daily precipitation data. The first one is a non-stationarity between calibration and application periods. New correction methods are developed, showing an increased resistance to non-stationary conditions. The second problem is related to the correction of a dependence (i.e. correlation and covariance) structure of multivariate precipitation data. A new procedure is proposed, correcting the complete dependence structure of the model data. All newly introduced methods are validated using measured and RCM-simulated data; the validation demonstrates their suitable applicability.

Application of mathematical models for simulation of hydrological conditions in selected streams
Kurková, Marie ; Vašků, Zdeněk (advisor) ; Michal, Michal (referee)
Flood is a natural phenomenon that occurs at different intensities and irregular time intervals. As to natural disasters, floods represent the greatest direct threat for the Czech Republic. They may cause serious critical situations during which not only extensive material damages are done, but may bring also losses of the lives of inhabitants in affected areas as well as vast devastation of cultural landscape including environmental damages. Important from the viewpoint of the elimination of potential threats and consequences of such events is the information issued by flood forecasting service about the character and size of flood areas for individual N-year flood discharges and specific flood scenarios. An adequate image of depths and flow rates in the longitudinal or cross profile of the watercourse during a flood event is provided by the hydrodynamic model. This is why the information obtained from the hydrodynamic models occupies a privileged position from the viewpoint of the protection of citizens' lives and mitigation of damage to their property. The first study is situated on the river Úhlava in meadows by Příchovice near the town Přeštice. The proposal of flood-protection measures is contained in Territorial control documentation. The documentation was elaborated on the basis of hydraulic calculations and experiences from the flood in August 2002. The mathematical model is practically used in the study of analysis of proposed flood-protection measures. The analysis is based on mathematical simulation of water outflow and water level on the river Úhlava. It is possible to use the non-commercial software Hec-Ras, version 3.1.1., for the simulation itself. One of the points of view of the possibility of using proposed flood-protection measures is total efficiency. The mathematical model is posssible to use as a basis of support for realization of proposed flood-protection measures on the river Úhlava in meadows by Příchovice within the grant programme "Program prevence před povodněmi II" under the control of the Ministry of Agriculture. In the second case the mathematical model is practically used in the study of hydrotechnical analysis of streams in cadastral unit. The analysis is based on matjematical simulation of water outflow and water level on chosen streams. It is possible to use the noncomercial software HEC-RAS for the own simulation. The analysis should be shown on dangerous places in the interest place. The mathematical model is possible of using to use as basis for revaluation of action in spatial plan or for view of the flood-protection measures in the village Mochtín. Basic input into the hydrodynamic models is represented by altimetry data. One of ways to obtain such data is through the method of aerial laser scanning (ALS) from the digital relief model (DRM). This method is considered one of the most accurate methods for obtaining altimetry data. Its bottleneck is however incapacity of recording terrain geometry under water surface due to the fact that laser beam is absorbed by water mass. The absence of geometric data on watercourse discharge area may perceptibly affect results of modelling, especially if a missing part of the channel represents a significant discharge area with its capacity. One of methods for eliminating the deficiency is a sufficient channel recess by means of software tools such as CroSolver. The third submitted paper deals with the construction of a hydrodynamic model using 5th generation DRM data, and compares outputs from this model at various discharges with a model based on the altimetry data modified by using the CroSolver tool. Outputs from the two hydrodynamic models are compared in HEC-RAS programme with the use of recessed data and with the use of unmodified DRM. The comparison is done on the sections of two watercourses with different terrain morphology and watercourse size. A complementary output is the comparison of inundation areas issuing from both model variants. Our results indicate that differences in the outputs are significant namely in the lower discharges (Q1, Q5) whereas for Q50 and Q100 the difference is negligible with a great role being played by morphology of the modelled area and by the watercourse size.

The analysis of the weather impact on the shape and shift of the production frontier
Hřebíková, Barbora ; Čechura, Lukáš (advisor) ; Peterová, Jarmila (referee)
Although weather is a significant determinant of agriculture production, it is not a common practice in production analysis to investigate on its direct impact on the level of final production. We assume that the problem is methodological, since it is difficult to find a proper proxy variable for weather in these models. Thus, in the common production models, the weather is often included into a set of unmeasured determinants that affects the level of final production and farmers productivity (statistical noise, random error). The aim of this dissertation is to solve this methodological issues and find the way to define weather and its impacts in a form of proxy variable, to include this variable into proper econometric model and to apply the model. The purpose of this dissertation is to get beyond the empirical knowledge and define econometric model that would quantify weather impacts as a part of mutually (un)conditioned factors of final production, to specify the model and apply it. The dissertation is based on the assumption that the method of stochastic frontier analysis (SFA) represents a potential opportunity to treat the weather as a specific (though not firm-controllable) factor of production and technical efficiency. SFA is parametric method based on econometric approach. Its starting point is the stochastic frontier production function. The method was presented in the work of Aigner, Lovell and Schmidt (1977) and Meusen and van den Broeck (1977). Unlike commonly used econometric models, SFA is based on analysis of production frontier that is formed by deterministic production frontier function and the compound error term. The compound error term consists of two parts -- random error (statistical noise, error term) and technical inefficiency. Technical inefficiency represents the difference in the actual level of production of the producer, and the maximum attainable (possible) level that would be achieved if the producer used a particular combination of production factors in a maximum technically efficient way. Over time, it has been developed on a number of aspects - see time variant and invariant inefficiency, heteroscedasticity, measurement and unmeasured heterogeneity. Along with the DEA, SFA has become the preferred methodology in the area of production frontier and productivity and efficiency analysis in agriculture. Lately, it has been applied for example by Bakusc, Fertő and Fogarasi (2008) Mathijs and Swinnen (2001), Hockmann and Pieniadz (2007), Bokusheva and Kumbhakar (2008) Hockmann et al. (2007), Čechura a Hockmann (2011, 2012), and Čechura et al. (2014 a, b). We assume that the weather impacts should be analysed with regard to technical efficiency, rather than as a part of statistical noise. Implementation of weather in part of deterministic production function rather than in the statistical noise is a significant change in the methodical approach within the stochastic frontier analysis. Analysis of the weather impacts on the changes in the level of TE has not been greatly recorded in the associated literature and is, therefore, considered as the main contribution of this work for the current theory of production frontier estimation, or the technological effectiveness, in the field of agriculture. Taking into account other variables that are important for the relationship and whose inclusion would enhance the explanatory power of the model was part of the objective of this work.Thus, the possible effect of heterogeneity was taken into account when models were formulated and final results discussed. The paper first defined and discussed possible ways how to incorporate the effects of the weather into production frontier model. Assessing the possibility of inclusion of weather in these models was based on the theoretical framework for the development of stochastic frontier analysis, which defines the concept of technical efficiency, distance functions theory, stochastic production function theory and the methodology and techniques that are applied within the framework of SFA, which were relevant for the purpose of this work. Then, the weather impacts on the shape and shift of production frontier and technical efficiency of czech cereal production in the years 2004-2011 was analyzed. The analysis was based on the assumption that there are two ways how to define variables representing weather in these models. One way is to use specific climatic data, which directly describe the state of the weather. For the purpose of this thesis, the variables mean air temperature (AVTit) and sum of precipitation (SUMPit) in the period between planting and harvest of cereals in the individual regions of Czech republic (NUTS 3) were selected. Variables were calculated from the data on monthly mean air temperatures and monthly sums of precipitation on the regional levels provided by Czech hydro-meteorological institute CHMI. Another way to define weather variable is to use a proxy variable. In this dissertation, the calculation of climatic index (KITit) was applied. Climatic index was calculated as a sum of ratios between the actual yield levels and approximated yield levels of wheat, barley and rye, weighted by the importance of each plant in a cereal production protfolio in each region of the Czech republic. Yield levels were approximated by the linear trend functions, yield and weights were calculated with the use of data on regional production and sown area under individual grains by year at the level of regional production (NUTS 3) provided by Czech Statistical Office. Both ways of weather definition are associated with some advantages and disadvantages. Particular climatic data are very precise specificatopn of the actual weather conditions, however, to capture their impacts on the level of final production, they must be implemented into model correctly along with the number of other factors, which have an impact on the level of final production. Climatic index, on the other hand, relates the weather impacts directly to the yield levels (it has been based on the assumption that the violation from yield trends are caused by the weather impacts), though, it does not accomodate the concrete weather characteristics. The analysis was applied on unbalanced panel data consisting of the information on the individual production of 803 producers specialized on cereal production, which have each the observations from at least two years out of total 8-years time serie. Specialization on crop production was defined as minimum 50% share of cereal production on the total plant production. Final panel consists of 2332 observations in total. The values of AVTit, SUMPit a KITit has been associated with each individual producer according to his local jurisdiction for a particular region. Weather impacts in the three specified forms were implemented into models that were defined as stochastic production frontier models that capture the possible heterogeneity effects. The aim is to identify the impact of weather on shift and shape of production frontier. Through the defined models, the production technology and technical efficiency were estimated. We assume that the proposed inclusion in weather impacts will lead to a better explanatory power of defined models, as a result of weather extraction from a random components of the model, or from a set of unmeasured factors causing heterogeneity of the sample, respectivelly. Two types of models were applied to estimate TE - Fixed management model (FMM) and Random parameter model (RPM). Models were defined as translogarithmic multiple-output distance function. The analyzed endogene variable is cereal production (expressed in thousands of EUR). Other two outputs, other plant production and animal production (both expressed in thousands of EUR) are expressed as the share on cereal production and they appear on the right side of the equation together with the exogene variables representing production factors labour (in AWU), total utilized land (in acres), capital (sum of contract work, especially machinery work, and depreciation, expressed in thousands of EUR), specific material (represented by the costs of seeds, plants, fertilisers and crop protection, expressed in thousands of EUR), and other material (in thousands of EUR). The values of all three outputs, capital, and material inputs were deflated by the the country price indexes taken from the EUROSTAT database (2005=100). In Random parameter model, heterogeneity is captured in random parameters and in the determinants of distribution of the technical inefficiency, uit. All production factors were defined as a random parameters and weather in form of KITit enters the mean of uit and so it represents the possible source of unmeasured heterogeneity of a sample. In fixed management model, heterogeneity is defined as a special factor representing firm specific effects, mi. This factor represents unmeasured sources of heterogeneity of sample and enters the model in interaction with other production factors and the with the trend variable, tit.Trend variable represents the impact of technological change at a time t for each producer i. The weather impacts in form of variables AVTit a SUMPit is, together with production factors, excluded from the set of firm specific effects and it is also numerically expressed. That way weather becomes a measured source of heterogeneity of a sample. Both types of models were estimated also without the weather impacts specification in order to obtain the benchmark against which the effects of weather impacts specification on production frontier and technical efficiency is evaluated. Easier interpretation of results was achieved by naming all five estimated models as follows: FMM is a name of fixed management model that does not include specified weather variables, AVT is a name for fixed management model including weather impacts in form of average temperatures AVTit, SUMP is name of model which includes weather impacts in form of sum of precipitations SUMPit, RPM is random parameter model that does not account for weather impacts, KIT is random parameter model that includes climatic index KITit into the mean of inefficiency. All estimated models fullfilled the conditions of monotonicity and kvasikonvexity for each production factor with the exception of capital in FMM, AVT, SUMP and RPM model. Violating the kvasikonvexity condition is against the theoretical assumptions the models are based on, however, since capital is also insignificant, it is not necesary to regard model as incorrect specification. Violation of kvasikonvexity condition can be caused by the presence of other factor, which might have contraproductive influence on final production in relation to capital. For example, Cechura and Hockann (2014) mention imperfections of capital market as possible cause of inadequate use of this production factor with respect to technological change. Insufficient significancy of capital can be the result of incorrect specification of variable itself, as capital is defined as investment depreciation and sum of contract work in the whole production process and not only capital related to crop production. The importance of capital in relation to crop production is, thus, not strong enough to be significant. Except of capital are all other production factors significant on the significancy level of 0,01. All estimated models exhibit a common pattern as far as production elasticity is concerned. The highest elasticity is attributed to production factors specific and othe material. Production elasticity of specific material reaches values of 0,29-0,38, the highest in model KIT and lowest of the values in model AVT. Production elasticity of other material reahed even higher values in the range 0,40-0,47. Highest elasticity of othe material was estimated by model AVT and lowest by model KIT. Lowest production elasticity are attributed to production factors labour and land. Labour reached elasticity between 0,006 and 0,129 and land reached production elasticity in the range of 0,114 a 0,129. All estimated models displayed simmilar results regarding production elasticities of production factors, which also correspond with theoretical presumptions about production elasticities -- highest values of elasticity of material inputs correspond with naturally high flexibility of these production factors, while lowest values of elasticity of land corresponds with theoretical aspect of land as relativelly inelastic production factor. Low production elasticity of labour was explained as a result of lower labor intensity of cereals sector compared to other sectors. Production elasticity of weather is significant both in form of average temperatures between planting and harvest in a given region, AVTit, and form of total precipitation between planting and harvest in a given region, SUMPit. Production elasticity of AVTit, reach rather high value of 0,3691, which is in the same level as production elasticities of material inputs. Production elasticity of SUMPit is also significant and reach rather high lower value of 0,1489. Both parameters shows significant impact of weather on the level of final crop production. Sum of production elasticities in all models reach the values around 1, indicating constant returns of scale, RS (RSRPM=1,0064, RSKIT=0,9738, RSSUMP =1,00002, RSFMM= 0,9992, RSAVT=1,0018.). The results correspond with the conclusion of Cechura (2009) and Cechura and Hockmann (2014) about the constant returns of scale in cereals sector in Czech republic. Since the value of RS is calculated only with the use of production elasticities of production factors, almost identical result provided by all three specifications of fixed management model is a proof of correct model specification. Further, the significance of technological change and its impact on final production and production elasticities were reviewed. Technological change, TCH, represents changes in production technology over time through reported period. It is commonly assumed that there is improvement on production technology over time. All estimated models prooved significant impact of TCH on the level of final production. All specified fixed management models indicate positive impaact of TCH, which accelerates over time. Estimated random parameter models gave contradicting results -- model KIT implies that TCH is negative and decelerating in time, while model RPM indicates positive impact of TCH on the level of final production, which is also decelerating in time. It was concluded, that in case that weather is not included into model, it can have a direct impact on the positive direction of TCH effect, which can be captured by implementing weather into model and so the TCH becomes negative. However, as to be discussed later, random parameter model appeared not as a suitable specification for analyzed relationship and so the estimate of the TCH impact might have been distorted. The impact of technological progress on the production elasticities (so-called biased technological change) is in fixed management models displayed by parameters representing the interaction of production factors with trend variable. The hypothesis of time invariant parameters (Hicks neutral technological change) associated with the production factors is rejected for all models except the model AVT. Significant baised technological change is confirmed for models FMM and SUMP. Biased technological change is other material-saving and specific material-intensive. In the AVT model, where weather is represented by average temperatures, AVTit, technological change is not significant in relation to any production factors. In both random parameter models, rejection of hypothesis of time invariant parameters only confirms significance of technological change in relation to final crop production. Nonsignificant effect of technological change on production elasticity of labor, land and capital indicates a generally low ability of farmers to respond to technological developments, which can be explained by two reasons. The first reason can the possible complications in adaptation to the conditions of the EU common agricultural market (eg. there are not created adequate conditions in the domestic market, which would make it easier for farmers to integrate into the EU). This assumption is based on conclusion made by Cechura and Hockmann (2014), where they explain the fact that in number of European countries there is capital-saving technological change instead of expected capital-using technical change as the effect of serious adjustment problems, including problems in the capital market.. Second possible reason for nonsignificant effect of technological change on production elasticity of labor, land and capital is that the financial support of agricultural sector, which was supposed to create sufficient conditions for accomodation of technological progress, has not shown yet. Then, the biased TCH is not pronounced in relation to most production factors. Weather impacts (SUMPit, AVTit) are not in significant relation to technological change. Both types of models, FMM and RPM were discussed in relation to the presence of the heterogeneity effects All estimated random parameters in both RPM models are statistically significant with the exception of the production factor capital in a model that does not involve the influence of weather (model RPM). Estimated parameter for variable KITit (0,0221) shows significant positive impact of the weather on the distribution of TE. That way, heterogeneity in relation to TE is confirmed, too, as well as significant impact of weather on the level of TE. Management (production environment) is significant in all three estimated fixed management models. In models that include weather impacts (AVT, SUMP), the parameter estimates indicates positive, slightly decreasing effect of management (or heterogeneity, respectivelly) on the level of final crop production. In model FMM, on the contrary, first and second order parameters of mangement indicate also significant, but negative and decelerating effect of management (heterogeneity) on final crop production. If weather impact is included into models in form of AVTit, or. SUMPit, the direction of the influence of management on the level of final crop production changes. Based on the significance of first order parameter of management, significant presence of heterogeneity of analyzed sample is confirmed in all three estimated fixed management models. As far as the effect of heterogeneity on single production factors (so called management bias) is concerned, the results indicate that in case of model that does not include weather impacts (model FMM) the heterogeneity has positive impact on production elasticities of land and capital and negative effect on the production elasticities of material inputs. In models that account for weather impacts, heterogeneity has negative effect on production elasticities of land and capital and positive effect on the elasticity of material inputs. Heterogeneity effect on the production elasticity of labor is insignificant in all models FMM. In all three estimated models, the effect of heterogeneity is strongest in case of production factors specific and othe material, and, also, on production factor land. In case of FMM model, heterogeneity leads to increase of production elasticity of land, while in AVT and SUMP heterogeneity leads to decrease of production elasticity of land. At the same time, the production elasticity of land, as discussed earlier, is rather low in all three models. This fact leads to a conclusion that in models that accomodate weather impacts (AVT and SUMP), as the effect of extraction of weather from the sources of unmeasured heterogeneity, the heterogeneity has a negative impact on production elasticity of land. It can be stated that the inclusion of weather effects into the sources of unmeasured heterogeneity overestimated the positive effect of unmeasured heterogeneity on the production factor land in the model FMM. Management does not have a significant effect on the weather in form of SUMPit, while it has significant and negative effect on the weather in form of average temperature, AVTit, with the value of -0.0622**. In other words, heterogeneity is in negative interaction with weather represented by average temperatures, while weather in form of the sum of precipitation (SUMPit) does not exhibit significant relation to unmeasured heteregeneity. In comparison with the model that does not include weather impacts, the effect of heterogeneity on the production elasticities has the opposite direction the models that include weather. Compare to the model where weather is represented by average temperature (model AVT), the effect of management (heterogeneity) on the production elasticity of capital is bigger in model with weather represented by sum of precipitations (model SUMP) while the effect of management (heterogeneity) on the production elasticity of land and material imputs is smaller in model with weather represented by sum of precipitations (model SUMP). Technical efficiency is significant in all estimated models. The variability of inefficiency effects is bigger than the variabilty of random error in both models that include weather and models where weather impacts are not specified. The average of TE in random parametr models reaches rather low value (setting the average TE = 54%), which indicates, that specified RPM models underestimate TE as a possible result of incorrect variable specification, or, incorrect assumptions on the distribution of the error term representing inefficiency. All estimated FMM models results in simmilar value of average TE (86-87%) with the simmilar variability of TE (cca 0,5%). Technological change has significant and positive effect on the level of TE in the model that does not specify the weather impacts (model FMM), with a value of 0,0140***, while in the models that include weather in form of average temperatures, or sum of precipitations, respectivelly, technological change has a negative effect on the level of TE (in model AVT = -0.0135***; in SUMP = -0.0114***). It can be stated, that in the model where the weather impacts were not specified, the effect of TCH on the level of TE may be distorted, because the parameter estimate implies also a systematic influence weather in the analyzed period. The effect of unmeasured heterogeneity on the level of TE is significant in all three estimated fixed management models. In models AVT and SUMP, heterogeneity has a positive effect on the level of TE (in AVT = 0.1413 and in SUMP =0,1389), while in the model that does not include weather variable the effect of heterogeneity on the level of TE is negative (in FMM =-0,1378). In models AVT and SUMP, the weather impacts were extracted from the sources of unmeasured heterogeneity, and so from its influence on the level of TE (together with other production factors weather becomes a source of measured heterogeneity). The extraction of the weather from the sources of unmeasured heterogeneity leads to change in the direction of heterogeneity effects on the level of TE from negative (in model where weather was part of unmeasured heterogeneity) to positive. The direct impact of weather on TE is only significant in case of variable AVTit, indicating that average temperatures reduce the level of TE (-0.0622**). Weather in form of sum of precipitations does not have a significant impact on the level of TE. It is evident that incorporating the effects of weather significantly changes the direction of the influence of management on the production of cereals and the direction of influence on the management of production elasticity of each factor in the final model. Analogically with the case of the influence of heterogeneity on the production elasticity of land, it is stated that the weather (included in sources of unmeasured heterogeneity) played a role in the underestimation of the impact of heterogeneity on the overall cereal production. Also, in case that weather was not extracted form the sources of unmeasured heterogeneity would play significant role in underestimation of the effect of heterogeneity on the level of TE. Based on the results of parameters estimates, and on the estimate of average values of TE and its variability, it is concluded, that the effect of inclusion of weather into defined models does not have significant direct impact on the average value of TE, however, its impact on the level of TE and the level of final crop production is pronounced via effects of unmeasured heterogeneity, from which the weather was extracted by its specification in form of AVTit a SUMPit. The analysis results confirms that it is possible to specify the impacts of weather on the shape and shift of production frontier, and, this to define this impact in a model. Results Aaso indicate that the weather reduces the level of TE and is an important source of inefficiency Czech producers of cereals (crop). The model of stochastic frontier produkction function that capture the weather impact was designed, thereby the goal of the dissertation was met. Results also show that unmeasured heterogeneity is an important feature of czech agriculture and that the identification of its sources is critical for achieving higher productivity and higher level of final output. The assumption about significant presence of heterogeneity in production technology among producers was confirmed, and heterogeneity among producers is a significant feature of cereal sector. By extracting weather from sources of unmeasured heterogeneity, the impact of real unmeasured heterogeneity (all that was not extracted from its sources) and the real impact of weather on the level of TE is revealed. If weather was not specified in a model, the TE would be overestimated. Model in form of translogarithmic multiple-output distance function well approximates the relationship between weather, technical efficiency, and final cereal production. Analysis also revealed, that the Random parameter model, which was applied in case that weather impacts were expressed as an index number, is not the suitable model specification due to underestimating of the average level of TE. The problem of underestimation of TE might be caused by wrong variable definition or incorrect assumptions about the distribution of inefficiency term. Fixed management model, on the other hand, appears as a very good tool for identification of weather impacts (in form of average temperatures and sum of precipitations in the period between planting and harvesting) on the level of TE and on the shape and shift of production frontier of czech cereals producers. The results confirm the assumption that it is important to specify weather impacts in models analyzing the level of TE of the plant production. By specification of weather impactzs in form of proper variables (AVTit, SUMPit), the weather was extracted from the sources of unmeasured heterogeneity. This methodical step will help to refine the estimate of production technology and sources of inefficiencies (or, the real inefficiency, respectivelly). That way, the explanatory power of model increase, which leads to generally more accurate estimate of TE. Dissertation has fulfilled its purpose and has brought important insights into the impact of weather on the TE, about the relationship between weather and intercompany unmeasured heterogeneity, about the effect of weather on the impact of technological change, and so the overall impact of weather specification on the shape and shift of production frontier. A model that is suitable application to define these relationships was designed. Placing the weather into deterministic part of production frontier function instead of statistical noise (or, random error, respectivelly) means a remarkable change in the methodical approach within the stochastic frontier analysis, and, due to the fact that the analysis of weather impacts on the level of TE to this extent has not yet been observed in relevant literature, the dissertation can be considered a substantial contribution to current theory of the estimate of technical efficiency of agriculture. The dissertation arose within the framework of solution of the 7th FP EU project COMPETE no 312029.