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Karyotype differentiation of Euscorpius scorpions (Scorpiones: Euscorpiidae) in Europe
Novotný, Tomáš ; Šťáhlavský, František (advisor) ; Ráb, Petr (referee)
The aim of presented work is to provide characteristics of the karyotypes of scorpions of the genus Euscorpius. Genus Euscorpius is a typical representative of scorpions in Europe. Its occurrence is wide throughout Europe. Until now, 18 species of this genus have been described. In this work six species were karyologically analyzed and one species was shown to possess only basic diploid number of chromosomes: E. carpathicus - 2n=90, E. concinnus - 2n=88, E. hadzii - 2n=68, E. sicanus - 2n=66, E. tergestinus - 2n=60, E. naupliensis - 2n=60, E. italicus - 2n=36. Description of the karyotypes revealed that all species studied exhibit achiasmatic meiosis; no presence of sex chromosomes was detected. The basic hypothesis of phylogenetic relationships and karyotype evolution of the genus Euscorpius was outlined. High interspecies variability in chromosome total count was found and by analysis of the 16S rRNA gene the taxonomic status of the species was confirmed. Hence, it seems that cytogenetic methods can contribute to the understanding of species diversity and differentiation of possible cryptic species within the genus Euscorpius.

Multidisciplinary care in hemodialysis centers.
VYHLIDALOVÁ, Mária
It is inconceivable for only one person to take care of ill people in a health care. To achieve the aim which is in particular maintenance of the highest life quality of specimens in health or in disease, is important mutual cooperation of professionals in the branch, where every single ensure care for a patient on the basis of his own knowledge and skills. It is about team co-operation, where every single member of the team has his own obligations, but also rights and authority. The term multisiciplinary care means co-operation of more scientific branches. Hemodialysis patients'nursing care is about team of professionals, who are fully involved in satisfying patients' needs. This co-operation enables primarily the unitary communication, reduction of patient's issue analysis, unified and consecutive nursing care, complete documentation and quality evaluation of provided nursing care of these patients. The aim of the dissertation was to chart multidicsiplinary care's issues at hemodialysis, on the basis of available literature. The dissertation is based on theoretically processed topic by the method of documents' content analysis and method of review and synthesis. Information is obtained from domestic but also foreign science books, scientific magazines, and internet sources. Findings from the 42nd international conference EDTNA/ERCA, which topic was Innovation of renal care through multi-professional leadership, which took place in Malmö, Sweden, from 31st of August to 3rd of September 2013, also contributed. The dissertation is divided into three basic chapters. The first part is focused on team characteristic. There are definitions, types, features, building, composition, roles, conflicts within a team and elemental description of multidisciplinary co-operation. The second chapter deals with a history and hemodialysis as a medical extracorporal method. The third, and the broadest section, is focused on multidisciplinary co-operation at the centre of hemodialysis. It is divided into several sub-chapters. In each of them the information descend from general field to concrete needs of hemodialysed patient and continue to the description of each member of a multidisciplinary team. The result is comprehensive view on provision of multidisciplinary care in the centres of hemodialysis. The output of the dissertation is a draft of the nursing care algorithm within the purview of multidisciplinary care at the centres of hemodialysis. Multidisciplinary care is necessary requirement for assumption of effective, high quality and continuous medical and nursing care at all the centres.

The current state and prospects for sustainable development of tourism in Slovakia.
Libantová, Silvia ; Indrová, Jarmila (advisor) ; Chaloupka, Radek (referee)
Tourism contributes to the prosperity of destinations by bringing financial resources, new jobs, a higher level of services and infrastructure development. However, if tourism does not develop sustainably, it is accompanied by negative factors, as well. Especially, negative impacts on the environment, such as: water and soil contamination, increased noise and overcrowded destination during the high season. As a response to the worsening state of the environment and possible irreversible changes caused by irresponsible human behavior, the idea of sustainable development is becoming more important. The theoretical part describes tourism in the Slovak Republic and highlights plans for sustainable tourism development. In the practical part, the results of the questionnaire, related to the awareness of sustainable tourism in the Slovak Republic, are evaluated. The main objective of this thesis is to evaluate the current situation in the field of sustainable tourism and to make suggestions for its future development.

Changes in structure and phase composition in the surface of tram rail
Švábenská, Eva ; Roupcová, Pavla ; Schneeweiss, Oldřich
We have investigated structure and phase composition of surface layer of tram rails after long time running and the results were compared with those obtained on the original part of material. Changes due to effects of severe plastic deformation together with thermal shocks by friction process were expected. The information about structure and phase composition was obtained by optical and scanning electron microscopy, X-Ray Powder Diffraction, Mössbauer Spectroscopy and Glow Discharge Emission Spectroscopy (GDOES) and this was completed by microhardness measurements. The results show that the surface layer in comparison with the original material exhibits important changes in grain structure, an increase in microhardness and high content of iron oxide and hydrooxides. According to the depth profile of the chemical composition measured by GDOES there is an increase in carbon content in the surface layer which can be effect of up-hill diffusion.

A Comparison of Preconditioning Methods for Saddle Point Problems with an Application to Porous Media Flow Problems
Axelsson, Owe ; Blaheta, Radim ; Hasal, Martin
The paper overviews and compares some block preconditioners for the solution of saddle point systems, especially systems arising from the Brinkman model of porous media flow. The considered preconditioners involve different Schur complements as inverse free Schur complement in HSS (Hermitian - Skew Hermitian Splitting preconditioner), Schur complement to the velocity matrix and finally Schur complement to a regularization block in the augmented matrix preconditioner. The inverses appearing in most of the considered Schur complements are approximated by simple sparse approximation techniques as element-by-element and Frobenius norm minimization approaches. A special interest is devoted to problems involving various Darcy, Stokes and Brinkman flow regions, the efficiency of preconditioners in this case is demonstrated by some numerical experiments.

STRAIN ENGINEERING OF THE ELECTRONIC STRUCTURE OF 2D MATERIALS
del Corro, Elena ; Peňa-Alvarez, M. ; Morales-García, A. ; Bouša, Milan ; Řáhová, Jaroslava ; Kavan, Ladislav ; Kalbáč, Martin ; Frank, Otakar
The research on graphene has attracted much attention since its first successful preparation in 2004. It possesses many unique properties, such as an extreme stiffness and strength, high electron mobility, ballistic transport even at room temperature, superior thermal conductivity and many others. The affection for graphene was followed swiftly by a keen interest in other two dimensional materials like transition metal dichalcogenides. As has been predicted and in part proven experimentally, the electronic properties of these materials can be modified by various means. The most common ones include covalent or non-covalent chemistry, electrochemical, gate or atomic doping, or quantum confinement. None of these methods has proven universal enough in terms of the devices' characteristics or scalability. However, another approach is known mechanical strain/stress, but experiments in that direction are scarce, in spite of their high promises.\nThe primary challenge consists in the understanding of the mechanical properties of 2D materials and in the ability to quantify the lattice deformation. Several techniques can be then used to apply strain to the specimens and thus to induce changes in their electronic structure. We will review their basic concepts and some of the examples so far documented experimentally and/or theoretically.

Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Kubáň, Pavel ; Boček, Petr ; Seip, K. F. ; Gjelstad, A. ; Pedersen-Bjergaard, S.
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel approach requires μL volumes of samples and organic\nsolvents, which form consecutive immiscible plugs in a transparent polymeric capillary\nand the extraction process is accelerated by application of d.c. voltage. Number of the\nplugs, their composition, volume and sequence in the extraction capillary can be chosen\nspecifically for each particular application and high variability and selectivity of the\nextraction process can be achieved. In this contribution, we demonstrate suitability of\na five-phase μ-EME system for simultaneous extractions of cations and anions from\nurine and for selective extractions of basic analytes based on their acid-base strength\nfrom plasma.

Sorption and Stabilization of Metals/Mettalloids by Innovative Synthesized Sorbent Amochar.
Ouředníček, P. ; Trakal, L. ; Komárek, M. ; Pohořelý, Michael
Remediation of contaminated soil which is based on stabilization and immobilization of potential\nhazardous substance by sorption materials has been studied intensively nowadays. Biochar – activated organic carbon belongs to this group of stabilizing agents which can adsorb wide range of contaminants, including metals/metalloids. Surface area of the biochars is quite large in general and functional groups (e.g. COO–) can form chelates or alkaline elements on the surface, which is represented by cation exchange capacity. Altogether with the high pH values (7.00 – 10.00), biochars are quite effective sorbents and can adsorb metals/metalloids from the solution (ground water), especially in acidic soils (in the environments affected by intensive mining activities). Sorption\neffectiveness can be increased (especially for As (V) or Cr (VI) sorption) by modification of biochar by various types of secondary oxides. Innovative sorbent AMOchar (AMO + biochar) has been synthesized currently. The product was prepared by adding of biochar to the reaction solution during amorphous manganese oxide (AMO) synthesis. The AMOchar was formed mainly by Mn-oxalates which had coated surface of the pristine biochar. AMOchar composite was able to remove significantly higher amounts of various metal(loid)s from the solution despite the rather high pH of the material. Sorption effectiveness was high not only in case of Pb (II) sorption (almost 99%), and Cd(II) (51.2%), but also a very high amount of As(V), 91.4%. Additionally, both AMOchar composite was able to reduce Mn leaching. This can avoid potential post-contamination caused by the dissolution of less stable Mn-oxalates as observed in the pure AMO.
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Plasma spraying from liquids: plasma liquid interaction and coating build up
Tesař, Tomáš ; Mušálek, Radek ; Medřický, Jan ; Lukáč, František
Plasma spraying from liquid feedstocks is a rapidly developing field of thermal spraying since the coatings prepared from liquids exhibit some unique features, such as high hardness, thermal shock resistance or low thermal and electric conductivity. The key factor influencing the final coating character and properties is the input material which may be in the form of a suspension or a solution. Parameters of the selected suspension (solids concentration, viscosity, surface tension, chemical composition, etc.) or solution (concentration, etc.) determine its interaction with the plasma jet which strongly influences the coating buildup. This proceeding introduces the problematics of the interaction between the liquid feedstock material with the plasma jet and presents the way of evaluation of the coating buildup.

Determination of content substances for selected species and varieties of roses.
Slavíková, Eva ; Sus, Josef (advisor) ; Súkeníková, Tereza (referee)
This bachelor's work is focused on comparing the most significant content substance, vitamin C, in fruits of chosen rose kinds species and varieties. Herbal products are often seen by people as products of "second choice", when failure of allopathic treatment occurs, which is focused especially on suppressing symptoms of illness, but it ignores health of the human body as whole. That's why are herbal products often alternative for therapy of chronic diseases. The base of this work is extensive literary research and results of laboratory measurings. Literary part of this work includes description of botanical classification of rose plants. There is stated basic characteristic of individual rose species and their morphological features. In following chapters is composed overview of effective substances in rose fruits and their effect on human health. In practical part of this work are included results of laboratory tests for determining ascorbic acid content for chosen samples of rose species and it also includes results of weighing and measuring of fruit samples of chosen rose species. There are also stated chosen rose species and shortly their origin and description, in the beginning of practical part. For practical part were chosen samples of species. Results of practical part are compiled into statistic tables and graphs and it confirms high content of ascorbic acid in rose fruits. This content is in range of cca 300 - 900 mg.100 g-1. Due to growing interest in alternative treatment options and "clean" natural product, it can be assumed, that many plants of Rosaceae family will keep its place in treatment practice. Those plants will be used not only for treatment of acute and chronic diseases, but also for cosmetic products production, thanks to its large effects and process options.