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Determination of levoglucosan in urban aerosol
Coufalík, Pavel ; Mikuška, Pavel ; Čmelík, Richard
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by LC-MS.

The Liberation of Pilsen 1945 in Photography
Křenová, Tereza ; SILVERIO, Robert (advisor) ; JANOŠČÍK, Václav (referee)
This bachelor thesis deals with liberation of Pilsen in May 1945 by american army in photography. The aim of this thesis is finding and collecting archival materials and placing them in a historical-political context. Communist regime changed the portrayal of historical events and directly influenced the amount of exisiting photographs, documents and lives of those who created them. Important part of this bachelor thesis is working with those archival materials – particularly with photographs capturing arrival of Americans and their stay in Czechoslovakia at the end of WW2. Both professional and amateur photographers created valuable photographs as they witnessed the liberation of Pilsen. These photographs became in spite of communist propaganda a clear evidence that Pilsen was liberated by american army.

Food supplements and iodine supplementation breastfeeding women
IVANOVÁ, Markéta
Iodine is a trace element that is essential for the production of thyroid hormones triiodothyronine and thyroxine. Iodine deficiency had in the past result in many serious diseases and disorders. At present is in the Czech Republic considered mastered. However, Iodine deficiency are vulnerable to specific groups of the population, including pregnant and nursing women, because of their need and the recommended daily dose of iodine is increased. Breastfeeding women should receive adequate amounts of dietary iodine for optimal saturation for themselves and their child. Otherwise, exposing themselves and their child risk of diseases and disorders of iodine deficiency. Increased supply of iodine can be replaced by eating foods rich in iodine. In the event that you cannot increase intake of iodine - rich food preferences in iodine, there are various supplements that contain iodine. The aim of this thesis was to evaluate the intake of iodine supplements and supplements in nursing women and breast-feeding women to assess the awareness for greater needs of iodine and food iodine sources. There were set up the three research questions: How many nursing women used to supply iodine supplements and supplements?, What is the awareness of breastfeeding women need more iodine? and What is the awareness of breastfeeding women about dietary sources of iodine? A quantitative research strategy was used for the implementation of the research. The method used for data collection was made by an anonymous questionnaire. A questionnaire was handed in a printed copy to pediatricians and gynecologists in the southern Bohemia. With the doctors help were invited nursing women to fulfill the questionnaire. Part of the women was invited to complete the questionnaire directly. The research sample consisted of 117 female respondents. The research shows that 62 % of breastfeeding women know why it is important to increased need for iodine during lactation. But only 9 % of women know all the risks of iodine deficiency on the mother mentioned in the questionnaire and only one new mother knows all risk of iodine deficiency for infants listed in the questionnaire. The recommended daily intake of iodine properly reported or estimated 40.2 % of women. It turned out that 46 % of respondents from the research group used food supplements and supplements containing iodine. Each of these respondents used only one preparation with iodine. The most commonly used products were: GS mamavit, a Smart baby and Femibion 400 and Gravital. 43 % of respondents had used a dietary supplement containing iodine from the beginning of pregnancy and in 57 % of the respondents were informed about food supplements through a doctor (gynecologist, pediatrician). The results showed that awareness of breastfeeding women about food sources of iodine is not good. 67 % of women know that settlement higher needs iodine during lactation is possible by eating foods rich in iodine, but all food sources of iodine in the questionnaires (sea fish, seafood and seaweed, iodized salt, and milk and dairy products) registered only one respondent. 39 % of women known as a rich source of iodine, seafood, seafood and seaweed, iodized salt , but none of them knew that the rich and a good source of iodine is also milk and dairy products. Based on the results of the work would be desirable to issue educational print disabled educational material that could be used to increase awareness of not only women who are breastfeeding, pregnant women as well as the higher of the recommended daily dose of iodine and what foods to include in your diet in order to pay the increased intake of iodine. Provide information on supplements containing iodine, which can add missing quantities of iodine, what products are available and what are the currently preferred by nursing women.

Activities and functioning of New web organization
Tyl, Jan ; PROKOP, Petr (advisor) ; SULŽENKO, Jiří (referee)
Abstract Bachelor thesis Activities and functioning of New Web organisation aims to detailed analysis of this culture network. What paths choose, how fulfills its visions, etc. Describe the formation of the organization, motivations and partial steps, which New web takes for its funcioning. How partial sections of this organism thinks and how cooperate. Also this thesis deals with organization structure of New web. Collectively write an overview of activities this network. Uncover sources of funding. All in relation with functioning of another culture networks in Europe. Concretely IETM, Trans Europe Halles and IN SITU organizations. In conclusion wants to outline future direction of New web organization.

Lyme borreliosis
Kohoutová, Lucie ; Jílek, Petr (advisor) ; Křivčíková, Lucie (referee)
Author: Lucie Kohoutova Title: Lyme borreliosis (Diploma thesis) University: Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Pharmacy Background: We investigated the presence of Lyme disease in the area of Havlickuv Brod based on laboratory diagnostics of specific or non- specific antibodies in the blood serum and in the liquor. The aim was to define typical laboratory signs of illness and recommend racional methods in diagnosis of Lyme disease. Methods: We used the data from Havlickuv Brod hospital laboratory of immunology. Patients were dividend into the groups according to the results of basic tests (if there were immunoglobulines type M and type G in serum or liquor), sex, age, the day of blood collection, code of diagnosis, patient's doctor and the type of running tests (ELISA, westernblot). These groups were compared. Patients with at least one positive result in basic tests and with any of additional test at the same time were divided in compliance with relations amongst these types of tests. We tried to determine appropriate interval between taking samples during monitoring the dynamics of the disease by contribution- based method. Results: More threatned by Lyme borreliosis are older women and people over the age of fifty- five. The highest number of new...

The influence of nutrient loading, meteorological and hydrological conditions and operating manipulations on phytoplankton in the water suply reservoir Římov.
Hejzlar, Josef ; Jarošík, Jiří ; Nedoma, Jiří ; Seďa, Jaromír ; Znachor, Petr
Analysis of data collected during long-term and complex limnologic monitoring of the Římov reservoir in the period 1983 – 2015, which depict the development of physico-chemical conditions, hydrology and hydrodynamics of the reservoir with links to biological data on phytoplankton, zooplankton etc. showed that the concentration and species composition of phytoplankton depend on the supply and availability of nutrients, but are also influenced by climatic and hydrological conditions and water management operation of the reservoir.\n

Population genetic analysis of Old Kladruber horse
Vostrá Vydrová, Hana ; Majzlík, Ivan (advisor) ; Karel, Karel (referee)
The Old Kladruber horse, along with the Lipizzaner horse, Andalusian horse and Lusitano horse, is of the original Italo-Spanish type. The Old Kladruber horse is kept in two colour varieties (grey and black). Because the population is closed, there is a concern about the loss of genetic variation. The genetic diversity and population structure were analysed in the Old Kladruber horse breed based on the pedigree information of animals that were registered in the Studbook to identify factors that may have affected the genetic variability of the breed. Pedigree records collected from 1729 to 2013 contained information on 7971 animals that were used in the analyses. The pedigree depth was up to 33 generations, with an average of 15.1 complete generations. The effective number of founders and ancestors contributing to the current genetic pool was 92.69 and 17.16, respectively. The average values of the inbreeding coefficient were as follows: 13% (with a maximum value of 29%) for the reference population (individuals that can currently take part in reproduction, n=612), 11% for the grey variety (with a maximum value of 25%) and 15% for the black variety (with a maximum value of 29%). The proportion of inbred animals was high (99%). The average rate of inbreeding in the reference population was 1%: 0.8% for the grey variety and 1.1% for the black variety, and the respective estimates of the effective population sizes were 52 for the reference population, 62 for the grey variety and 45 for the black variety. The estimated percentage of genetic diversity lost due to non-random mating within subpopulations and the reference population was 1.0, 1.0 and 1.2%, respectively. The total loss of genetic diversity in the reference population, in the grey variety and in the black variety was 11%, 13% and 17%, respectively.

Individual human odor as a forensic trail in criminal proceeding made by contact or contactless transmission and its resistance to physical agents
Santariová, Milena ; Bartoš, Luděk (advisor) ; Chmelíková, Eva (referee)
The dissertation thesis consists of four papers that have been published in scientific journals. Study n. 1 The need to recover evidence from water is quite common in criminal investigation. The article deals with the possibility to collect human scent from such objects and with the ability of specially trained dogs to match such scent samples with scent samples collected from detained suspects. During an experiment, designed as a blind one, it has been proved, that human scent can survive on submerged objects and can be later used for scent identification. Study n. 2 To collect odors the Czech Republic Police use special fabric sorbents manufactured under the registered mark ARATEX. Before use the fabric sorbent is treated by water vapor sterilization. After the scent identification the sorbent is destroyed. The goal of the study was to verify if the vapor sterilization is effective enough to remove human scent that has already penetrated into the sorbent structure or in other words if the sorbent can be exposed to vapor sterilization and then used again. Specially trained dogs were used to match starting odors with target odors in the line-ups. The results showed that dogs are able to correctly match human odors even after they have being exposed to vapor sterilization. Study n. 3 The purpose of the study was to determine the temperature at which the human scent is degraded so that a dog would not be able to identify it. In contrast to expectations, eight dogs used in the experiment almost flawlessly identified human scents from five scent donors exposed to temperatures of 100°C, 200°C, 300°C, 400°C, 500°C, 600°C, 700°C, and 800°C. Only two of the dogs were able to identify 5 of 15 scent samples exposed to 900°C. No dog identified a scent exposed to 1000°C. Our study verified heat survivability of human scent far beyond existing expectations. Study n. 4 We tested the hypothesis that if odor fallout (the release of a human odor onto an untouched object) in human subjects exists, then holding a hand above an absorbent will produce a detectable scent which will be subsequently matched in a detection test by trained dogs. Scents were collected from seven males to sterile cotton absorbent squares. The left hand was used to get the control scent and the right hand served as the target scent. Each experimental subject was sitting and his left hand was laid down on a cotton square for 3 min. The right hand was held 5 cm above another cotton square for 3 min. The scent identification was done by two specially trained police German shepherds. Both dogs performed 14 line-ups and correctly matched the collected scents of all test subjects. The results suggest the existence of human odor fallout, whereby a human scent trace is left by humans even if they do not touch an object.

Total contents and speciation of arsenic and selenium in plants growing in soils with different physico-chemical properties
Tremlová, Jana ; Száková, Jiřina (advisor) ; Vymazal, Jan (referee)
This dissertation thesis deals with uptake, accumulation and transformation of arsenic and selenium in plants growing on soils with different chemico-physical soil properties. The contents of arsenic were investigated in 99 wild plant species, coming from 27 different families and 6 different vegetables growing in arsenic contaminated soils. The results suggest that there are species-specific mechanisms which protects certain plants from the excessive uptake of arsenic from the soil. On the other hand, there are plants using a strategy of accumulation, which theoretically may increase the risk of arsenic entry into the food chain, whether through grazing cattle and wildlife or through consumption of vegetables growing on arsenic contaminated soils. The most prevalent arsenic compounds are AsV and AsIII. Other arsenic compounds occure as minor species. An important finding was detection of arsenobetaine in Plantago lanceolata L. and Carex praecox Schreb .. The contents of selenium were examined in 73 species of wild plants, coming from 29 different families. The results suggest that naturally low levels of selenium in the soil of selected locations along with a low capability of selenium uptake via collected plant species lead to the low content of selenium in plant biomass which may contribute to selenium deficient throughout the food chain. Foliar application of selenate on wild plant communities and on some types of vegetables in our case Brassica oleracea var. italica can have a positive impact on increasing the selenium content in the aboveground biomass of these plants and by extension, increase the selenium content in the human diet. Dominant selenium compounds in the aboveground biomass of the analyzed plants were SeVI and selenomethionine, with variable proportion of other commonly occurring organic selenium compounds, which is mainly affected by plant species.

Collective invesment
Černý, Štěpán ; Veselá, Kamila (advisor) ; Jitka, Jitka (referee)
The main object of this Diploma Thesis is according to collective investment through mutual funds formulate appropriate portfolio model for different types investors of retail banking. Theoretical part of the thesis is focuses on information and rules of investements, advantages and disadvanteges of collective investmentm fund types and their strategies. The practical part parses the selected mutual funds and then compares according to historical performance and riskiness. In the last part of it is handled by the main target. In this section are created model portfolia for individual investors, which are diversified and selected investment companies. Each of the suggestions and recommendations of the model portfolios correspond to the principles of conservative, balanced and dynamic investor.