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název v anglickém jazyce není uveden
Kolesová, Hana ; Grim, Miloš (advisor) ; Slípka, Jaroslav (referee) ; Nečas, Emanuel (referee)
Branchial arches region and its blood vessels are extensively transformed in the embryonic development. Aim of this study is to investigate mechanisms of the branchial arches region development and to study how a morphogen Sonic hed gehog (Shh) participate on the formation and remodeling of branchial arches and their blood vessels. Influence of Shh was evaluated based on the changes caused by its inhibition in vivo. Shh function was inhibited with an anti-Shh antibody, which was produced into the embryo from the applied hybridoma cells. Shh signaling cascade was also inhibited by cyclopamine. Results show that Shh is important for dc novo format,ion of the blood vessels in the branchial arches region. Further Shh is necessary for stabilization of the vessel wall, mainly for anterior cardinal vein. Shh also alfects later vessel development and transformation, which includes i.e. fusion of the dorsal aorta, branching of the internal carotid artery and outflow tract development. Short time inhibition of Shh has minor effect on the apoptosis and proliferation activity of the branchial arches region mesenchymal cells. We assume that Shh signals directly to the blood vessels endothelial cells, as Shh receptor ptel is also expressed in endothelial cells and its signal is reduced with Shh inhibition. In studied...
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Glycocalyx shedding by cercariae of bird schistosomes
Chaloupecká, Jana ; Mikeš, Libor (advisor) ; Štěrba, Ján (referee)
Trichobilharzia spp. are avian schistosomes related to medically important human parasites of the genus Schistosoma. Penetrating cercariae are well known as causative agent of cercarial dermatitis in humans. Cercariae actively penetrate the skin of definitive hosts and transform into schistosomula. This process is preceded by cercarial tail detachment and includes emptying of penetration glands and extensive surface changes. One of these changes is the loss of highly immunogenic glycocalyx which represents a protective coat in the aquatic environment. The glycocalyx has specific composition of saccharide molecules which are bound to lipids or proteins on the membrane of cercarial tegument. There is only limited information about the mechanism of shedding. Hypotheses based on indirect evidences suggest that peptidases or (phospho)lipases from penetration glands could be involved. This work describes the changes in surface glycosylation during transformation of cercariae into schistosomula by fluorescently labelled lectins and monoclonal antibodies against Lewis X antigen. Lectins UEA-I, LTA and PNA have been chosen as markers of transformation of T. regenti. Further, our experiments have been focused on shedding of cercarial glycocalyx. During in vitro induction of penetration gland emptying and...
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Biomarkers in Myocardial Damage Diagnostics
KOPŘIVOVÁ, Olga
Anotace (abstract) Biomarkers diagnosing myocardial damages have recently been a very dynamically developing part of clinical biochemistry. Implementing of new biomarkers is very prompt; they are mostly implemented into practice without wider clinical and analytical experience. During a few years these are new methods to determine myoglobin, troponins (cTnI, cTnT), CK-MB mass, BNP etc. The effort of clinical biochemists and cardiologists is to cover all phases and levels of myocardial damage in acute coronary syndromes by means of cardio markers. These investigations are dramatically changing the view of diagnosis, determination of prognosis and therapy in acute coronary syndromes. In my thesis I covered retrospective data analysis in patients with the pain in the chest, presented at an inpatient clinic. The value of myocardial damage indicators and their dynamism was analysed from the definitive diagnosis point of view. I performed my own analysis at OKB Nemocnice Písek, a.s. (Hospital Písek, Inc.). The aim of my thesis was to cover the significance of particular myocardial damage markers and find out if investigated set of markers is sufficient for correct and prompt diagnosis of acute myocardial infarction. In terms of this thesis I tried to find out if the panel of cardiac markers contains excess values. In OKB Písek, a.s. the panel of myocardial damage markers includes: myoglobin, troponin I, CK-MB mass and the marker of heart failure BNP. In the samples of fifty-six patients I analysed the presence of the above mentioned markers of myocardial damage and heart failure. I examined only the markers which were determined by a physician. In the group of investigated patients with chest pain, measured values of troponins proved myocardial infarction in sixteen patients, values of CK-MB mass in sixteen patients and values of myoglobin in twelve patients. In the control group of twenty patients with chest pain the boundary values of troponin I were measured in two patients, without the increase of other myocardial damage markers. High value of troponin I was measured in two patients in non-ischemic myocardial damage (acute pericarditis), which agrees with data in scientific literature. The thesis shows that the level of troponin I reflects myocardial damage relatively accurately; troponin I is sufficiently cardio specific but is not a sufficiently early marker. For early management of acute myocardial infection it is important to determine myoglobin, which is detectable within two hours since the beginning of troubles but is not sufficiently cardio specific. The course of CK-MB mass elevation wave copies the course of troponin I elevation wave. That is why the investigation of this marker appears to be excessivet at current investigation of troponin I and myoglobin to prove myocardial damage, which is in agreement with scientific association recommendations.
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Dance Therapy as Help in Integration of Peoplewith Cerebral Palsy
Šourková, Barbora ; Ortová, Marie (advisor) ; Olšiaková, Jasmína (referee)
Dance therapy is a form of psychotherapy where move is the main mean of change. A man uses its own body for this change because body is physical aspect of personality. This therapy helps to create positive image of myself. It is a new profession which is still in proces of defining itself. Dancing education and art performance in this therapy is not the aim. In this therapy the esthetic aspect of movement is not concidered. The Greek word therapy has the same meaning as Czech word accompany. Therapist is the one who accompanies client on his way to recovery and self-knowledge. The principial moment of dance therapy is connection of body movement and emotional experience. Connection body-mind is easy and harmonical functioning systém. Discruption continuity of this system can point out psychological and physical stress. Relation between emotion and movement charakterize an individual. That speaks about face expression, posture, space largeness, where the body moves etc. If psychotherapy changes psychical attitude, even the physical standard will change. In dance therapy thisprincipal also works but in revers order. Participant of this change of mans behaviour is his body. The fact that physical comunication becomes less important due to speach development is also surfaced. It is obvious that animals are in...
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The civil elites at the local level
Koutná, Gabriela ; Müller, Karel (advisor) ; Fleissner, Kamil (referee)
The main objective of this thesis is the identification and understanding of the mechanisms of recruiting local political elites and their role in the process of democratization of local politics, and in the process of strengthening local civil society on concrete example selected municipality. The case study concerns with the municipality Semily, which was chosen to work as a community with exemplary transparent and open manner of governance. In 2006 there was a significant change in political representation when recruiting new political elites was a top down process led by a particular person according to predetermined priorities.
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Community Aproach to the People with Mental Health Problems As Alternative to the Institucional Care
Strnadová, Dagmar ; Klimešová, Anna (advisor) ; Sivek, Vojtěch (referee)
In the history the care of people with a mental health problems was going through a lot of changes. Most of the people were accepted as a part of a society. The family and a local community had been taken care of them. Only a small part of people with a mental health problems had been banished or kept in prison. With the beginning of a modern period the people with a mental illness have devoted and segregated in a large extent. The psychiatri and care for disordered people occured rapid developement in 20th century. In the 50's the psychiatric institution had been closing down in a many advanced countries. Institutional care was replaced with a out-patient and comunity care services. The main target of a community care is to help to the people with a mental illness so they can live in a naturally neighbourhood. The Great Britain has ranked among countries with a along tradition of community care. The broad range of services in the domain of living, employment and a leisure time arised here. The attitude to disordered people was also changed. In the Czech republic the community work set up to apply in 90's. Since the time the big progress was reached. Nevertheless the institutional care is still being preferred. The community care concur at traditional community values and it is established on esteem to...
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Aspects of Gene Regulation of CYP3A4 in Hepatic Tissue.
Krausová, Lucie ; Štaud, František (advisor) ; Mičuda, Stanislav (referee) ; Skálová, Lenka (referee)
1 Summary CYP3A4 is an important enzyme involved in elimination of majority of metabolized xenobiotics. It plays a major role in the detoxification system of the human body, therefore it is responsible for many drug-drug interactions (DDIs). DDI present a complication of current pharmacotherapy, in the extreme they can lead in failure of therapy or in life-threatening toxic effects. DDIs are caused by changes in enzymatic activity of CYP3A4, which is highly variable among individuals. An important mechanism of modulating CAP3A4 activity is the regulation of inducible transcription by nuclear receptors, especially PXR, CAR and GR. The structure of CYP3A4 promoter and mechanisms of transcriptional regulation has been studding intensively for many years, but the research of relationship of nuclear receptors and transcriptional cofactors in CYP3A4 transactivation is still incomplete. Present work contributes to elucidation of some questions concerning the effects of azole antimycotics on CYP3A4 transcription via PXR, potency of valproic acid to activate PXR and CAR or determinants of CYP3A4 expression via GR in placental cells. The experiments were performed with up-to-date molecular biology methods and using in vitromodels of the primary human hepatocytes and hepatoma cell lines. To the aims of the doctoral...
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INFLUENCE OF LASER CUTTING AND PUNCHING ON MAGNETIC PROPERTIES\nOF ELECTRICAL STEEL M470-50A
Bulín, Tomáš ; Švábenská, Eva ; Hapla, Miroslav ; Ondrůšek, Č. ; Schneeweiss, Oldřich
Electrical steel M470-50A belongs to the most often used materials in electrical machines. Due to this fact, it is desirable to know the magnetic parameters after processing raw sheets into the required shape. Basic parameters of mechanical, electrical, and magnetic properties of the sheets are usually obtained from the producer but the magnetic properties are changing in dependence on additional machining processes. The aim of this study is to describe changes in parameters of magnetic behavior after punching, laser and spark cutting of the original sheets. The basic information of structure was obtained by optical and scanning electron microscopy. The magnetic parameters were acquired from the measuring of magnetic hysteresis loops in dependence on saturation fields and frequencies. The results are discussed from the point of view of applied\ncutting technology with the aim to obtain the best magnetic parameters and consequently a higher efficiency of the final product. Results can be used as input parameters in simulation of the electrical machine.
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STRAIN ENGINEERING OF THE ELECTRONIC STRUCTURE OF 2D MATERIALS
del Corro, Elena ; Peňa-Alvarez, M. ; Morales-García, A. ; Bouša, Milan ; Řáhová, Jaroslava ; Kavan, Ladislav ; Kalbáč, Martin ; Frank, Otakar
The research on graphene has attracted much attention since its first successful preparation in 2004. It possesses many unique properties, such as an extreme stiffness and strength, high electron mobility, ballistic transport even at room temperature, superior thermal conductivity and many others. The affection for graphene was followed swiftly by a keen interest in other two dimensional materials like transition metal dichalcogenides. As has been predicted and in part proven experimentally, the electronic properties of these materials can be modified by various means. The most common ones include covalent or non-covalent chemistry, electrochemical, gate or atomic doping, or quantum confinement. None of these methods has proven universal enough in terms of the devices' characteristics or scalability. However, another approach is known mechanical strain/stress, but experiments in that direction are scarce, in spite of their high promises.\nThe primary challenge consists in the understanding of the mechanical properties of 2D materials and in the ability to quantify the lattice deformation. Several techniques can be then used to apply strain to the specimens and thus to induce changes in their electronic structure. We will review their basic concepts and some of the examples so far documented experimentally and/or theoretically.
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Cultural change in High Himalaya. The transformation of Sherpa and Thakali culture from the traditional to the modern context
Thoma, Michal ; Rychlík, Jan (referee) ; Soukup, Václav (advisor)
This diploma work is concerned with the cultural change in the societies of Sherpas and Thakalis of High Himalaya. Focus is on the crucial period - turn of fifties and sixties of the last century - when the Tibetan frontier was closed for the traditional long-distance trade. This trade was crucial for the living of the studied societies. This work at first brings brief geographical and historical overview of the situation in Himalaya and in general terms describes the transhimalayan trade system. The processes of building ethnic identities in Nepal Himalaya are explained thoroughly to facilitate understanding of various relations of the studied communities. In two chapters concerned with Sherpas and Thakalis the work deals with the traditional way of living of the studied societies, especially focusing on their adaptation to the harsh highland climate. Further it explains swift changes to which both ethnics was exposed and concentrates on the adaptive strategies and mechanisms employed to succeed in the new context. In the conclusion the work brings evaluation and comparison of the adaptive strategies of the studied groups.
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