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The Pump with a Diffuser with Stochastic Blades (Runner and Diffuser)
Dobšáková, Lenka ; Melichar, Jan (referee) ; Veselý, Jindřich (referee) ; Haluza, Miloslav (advisor)
This doctoral thesis studies the non-uniform blade cascade for centrifugal pumps. Influence of non-uniform blade cascade is investigated on the impeller and diffuser. A non-uniform blading impeller of pumps in this doctoral thesis was examined in terms of influence on efficiency characteristic. The idea of using non-uniform blading is to obtain a flat efficiency characteristic. Then the centrifugal pump could be used on larger scale of power with achieving a sufficiently high efficiency. The work is based on experimental measurements and numerical simulations of non-uniform hydraulic pump components. Non-uniform impeller and the diffuser were newly designed for this research. A new measuring track of small submersible pumps was needed to design and build for measurements. Different pump combinations with conventional hydraulics on one working point and hydraulics with non-uniform were measured. All measurements were evaluated and characteristics of efficiency, head and power were obtained for each measured combination. Some of pump combinations (which were measured in experiment) were numerically simulated by program Ansys Fluent. Data needed to evaluate the characteristic of efficiency and specific energy were collected from each numerical simulation. A theoretical consideration concerns the influence of non-uniform blade cascade applied on diffuser. The suggestion how to decrease hydraulic losses in canals was done. The theoretical consideration how to make a flat efficiency characteristic was made.
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Probability based method of reinforced concrete member approach
Sigmund, Václav ; Šimůnek, Petr (referee) ; Laníková, Ivana (advisor)
This thesis deals with the assessment of reinforced concrete elements using a fully probabilistic approach and its comparison with the method of partial reliability factors. This method of ultimate limit state design will be applied to determine the ultimate limit state of reinforced concrete column loaded by a combination of "N + M", the normal force and bending moment. Specifically, I will solve a circular reinforced concrete column with and without the effect of wrapping unidirectional carbon fiber fabrics and comparing the theoretically determined values with the values determined by experiment, which is carried out at the Institute of Concrete and Masonry Structures Faculty of Civil Engineering of the Technical University in Brno.
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Nové postupy biomonitoringu cizorodých látek ve vodním prostředí
ČERVENÝ, Daniel
Within the presented work, several approaches of biomonitoring were studied. A well-established method for human health risk assessment was applied to evaluate the quality of fish from open waters in the Czech Republic. As this work is targeted on sport fishermen, who frequently consume their own catches, besides the publication in scientific journal, a brochure with results of this study was distributed via the Czech Fishing Union. Health risks for consumers related to the consumption of wild fish were found strongly species and locality dependent. As there is no risks related with the consumption of carp (Cyprinus carpio) at all investigated sites, frequent consumption of predatory fish should be avoided at some fishing grounds. The use of passive samplers has become more and more attractive in the last two decades. As these devices are able to mimic the biological uptake of chemicals, their potential for replacing fish as bioindicators in routine biomonitoring programmes is evident. In our study, the comparison between analysis of fish tissues and POCIS extracts from selected sampling sites confirms this idea about interchangeability of these indicators in the case of PFASs as target pollutants. Moreover, no metabolic transformation of contaminants present in water take place in passive samplers contrary to living organisms, thus these reflect the actual contamination at the locality more precisely. The use of passive samplers completely fulfils the internationally accepted principles of Replacement, Reduction, Refinement (the three R´s). Another promising approach using YOY fish as bioindicators was evaluated within the present study. Multispecies samples of YOY fish homogenate was found as a better indicator for wide range of pollutants than muscle tissue of adult fish within one species from the same locality. As all inner organs and tissues are presented in analysed samples, more target pollutants in higher concentrations are detected compared to the muscle of adults. Besides the higher sensitivity in pollutants detection and quantification, practical and economic benefits are of concern in the case of the YOY fish approach. The YOY fish approach also particularly fulfil the three R´s strategy, as sampling of this age group of fish has a smaller impact on the environment than the sampling of adults. In case of mercury, that was found as a limiting contaminant for the consumption of fish from open waters in the Czech Republic, a nonlethal method of finclips sampling can be used for the monitoring of this compound in aquatic environments. Besides the monitoring, our optimised method for the estimation of muscle tissue concentrations could be used for human health risk assessment as well. The difference between real muscle concentrations and concentrations estimated using our method differs less than 10% in most of the investigated localities. Benthic organisms are an important part of the food web in aquatic environments, but insufficient information about its contamination is available. In another study included in this thesis, first evidence about bioaccumulation of certain pharmaceuticals in benthic organisms was described. Although, pharmaceuticals are generally considered to be not accumulative in organisms, our study proved that certain pharmaceuticals have bioaccumulation potential. From this finding, it is evident that not only the concentrations of pharmaceutical dissolved in water but also the diet represents an important exposure pathway of contamination by these compounds for organisms (fish) inhabiting aquatic environments.
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Economy of car sharing
Prokop, Vladimír ; Škubna, Ondřej (advisor) ; Jana, Jana (referee)
This thesis examines the issue of sharing of transport, namely the form car-sharing. Work defines the basic concepts of car-sharing, it advocates the concept of economic relations in line with the sustainable development of transport and describes the situation of car-sharing in the Czech Republic. The work also in the application part use the survey and looks to the usability of the cooperative model of car-sharing among students and younger workers in the Czech environment, and then compares this model with other modes of transport, from an economic perspective.
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Formation of yield and dry matter with genetic resources of opium poppy (Papaver somniferum L.)
Slowiaczek, Lukáš ; Hnilička, František (advisor) ; Jan, Jan (referee)
Diploma thesis with the topic: Formation of yield and dry matter with genetic resources of opium poppy (Papaver somniferum L.) was worked out at the Department of botanics and plant physiology, Faculty of agrobiology, food and natural resources ČZU in Prague. The purpose of this thesis was to determine genotype and ontogenetic differences in amount of yield, production of dry mass, and value of growth-analytic characteristics in certain species of opium poppy. Diploma thesis was based on the fact that most of the studies about opium poppy (Papaver somniferum L.) are focused on the content of alkaloids and other substances present in poppy seeds. Due to this facts, it is necessary to describe the genotypes of poppy plants and their genetic resources from the physiological point of view.
Following species of opium poppy were chosen for the experiment: Akvarel, Albín, Buddha, Florian, Korneuburger Weisser, Lazur, Major, Marianne, Opál, Postomi, Sokol, Tatranský. These species were chosen according to their content of alkaloids, especially morfine very low content, low content, medium content and high content of morfine. All of the tested genotypes are tested as object of study of genetic poppy resources. Seeds were supplied by GB Oseva Pro s.r.o., o.z. VÚO Opava and company Český mák.
Field experiments were established at the research laboratory FAPPZ ČZU in Prague, in Červený Újezd. This location is in Prague-West division, in area of Middle Bohemia. The altitude of this location is 401 metres above the sea level. The size of the field for testing was 2 m2. The experiment was based in 4 repetitions by the method of Latin square. Winter wheat was used as the preceding crop. Opium poppy was planted according to agrotechnical principles of planting.
Content of dry mass in individual plant organs was measured in certain ontogenetic stages of opium poppy (35, 45, 49, 52, 54, 62, 81 BBCH). Size of leaf area was assessed by the image analysis WinDias. Yield of seeds was assessed as well. Growth-analytic characteristics were calculated from the values of wieght of dry mass and size of leaf area.
The results confirmed that the size of plants is influenced by its ontogeny and also by its species. The results also confirm, that the height of plants is not influenced by the content of morfine. Connection between the colour of seeds and height of plant was not confirmed either. Increase in weight of above-ground biomass depending on ontogenetic stage and influence of plant species was confirmed. There are differences in production of yield in species of poppy with different contents of morfine because the average weight of yield in species with very low content of morfine was 112,36 g, in species with low content of morfine 110,41 g, in species with middle content of morfine 12167 g and in species with high content of morfine 123,16 g. It was approved that ontogeny of plants influences the leaf area index (LAI) as it increases until blooming and then it decreases as a result of senescence and falling off the old leaves. Differences in leaf area index were detected among the species of opium poppy, depending on the content of morfine. It was detected that crop growth rate (CGR), relative crop growth rate (Rw) and relative growth rate of assimilation area (RA) are influenced by the ontogeny of plants. It was detected that species with high content of morfine in poppy seeds (Buddha, Postomi, Lazur) and species with white seeds Sokol and Albín have lower yield than species with middle content of morfine.
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Evolutionary Ecology of Termites
Stiblík, Petr ; Šobotník, Jan (advisor)
Termites are one of the most important ecological factors on Earth. By right, they are called ecosystem engineers. They influence their habitat by very complex ways - recycling of plant tissues, aerating soil, transporting huge amounts of organic matter. Thanks to their ability decompose up to 100% of local production of celulose and lignin, they are responsible for termination of coal origination. Their ecological and evolutionary succsess is based on organization of their eusocial community into several different castes. Precize distribution of tasks between castes is one of the crucial factors of evolutionary success of termites. It is the undertanding of their ontogeny and colony organization what is key to effective fight against pest species and, on the opposite, the key to effective help to those species important for decomposing and transport of organic matter in soil.
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Mophological and molecular diversity of a tropical tree species Guazuma crinita in the Peruvian Amazon
Tuisima Coral, Lady Laura ; Lojka, Bohdan (advisor) ; Helena, Helena (referee)
Fast growing tree species Guazuma crinita (Malvaceae) was selected as a priority species for domestication in the Peruvian Amazon due its important contribution to the livelihood of local farmers. Its domestication process is still in an early age as for many tropical tree species little is known about its genetic variability and we dont know anything about the impact of domestication on its genetic resources. The main objective of this research was to assess the genetic variability of G. crinita within and among populations in the Peruvian Amazon by the use of morphological (wood physical traits) and molecular (ISSR and AFLP) markers.
Wood physical properties among six G. crinita provenances were evaluated. Wood samples were drilled from the base middle and top of the stem of 12 randomly selected eight-years-old trees for determination of wood measurement. Pearson correlations between physical properties were also determined. All wood physical properties except green density differed significantly among provenances. We also found statistically significant variation due to stem level position. The moderately dense wood and the coefficient of anisotropy (1.6) suggested that G. crinita has stable wood; they represent important advantages in terms of costs for transport and transformation process. The results suggested potential to select provenances with desirable wood properties for further breeding and domestication. Due to the variation found even in limited tree samples it is recommended further analysis with more extensive number of samples from different provenances and planting zones.
This research presents the first assessment of genetic variability based on inter-simple sequence repeat (ISSR) markers for 44 G. crinita genotypes from a clonal garden multiplication established in the Peruvian Amazon Research Institute (IIAP) in Ucayali region. Ten ISSR primers amplified a total of 65 bands of which 61 were polymorphic (93.8%). The range of DNA amplification varied from 260 to 2200 bp. Among the provenances overall genetic differentiation (Gst) was 0.03 indicating 97% of genetic diversity within provenances. Gene flow (Nm) was 12.9 alleles per generation. Cluster analysis was not related with geographic origin suggesting a common gene pool which was supported by calculation of weak positive correlation was found between genetic and geographic distance.
With the use of AFLP markers an insight on how domestication process does impact G. crinita genetic resources is also reported on this research work. I was able to generate fingerprint for 58 leaf samples representing eight provenances and three population types, 19 from a natural regenerated population 15 cultivated in home garden nursery and 24 from a collection of genotypes considered as semi-domesticated population. Seven selective AFLP primer combinations were used. A total of 171 fragments were amplified with 99.42% of polymorphism at species level. Each type of population generated fragments with 72.51% 49.12% and 54.39% of polymorphic fragments respectively. Neis genetic diversity and Shannon index information were found to be higher in the population of natural regeneration compared to overall semi-domesticated population (He = 0.10 and 0.9; I = 0.19 and 0.16 respectively). The analysis of molecular variation (AMOVA) showed higher variation within provenances rather than among (84% and 4% respectively). UPGMA clusters analysis and PCoA did not showed correspondence between genetic and geographic distance in addition their correlation was not significant. There was a significant genetic differentiation among types of population suggesting slight genetic bottleneck in semi-domesticated populations yet with relatively high levels of genetic variation.
In situ conservation for populations with high levels of genetic diversity was recommended. In addition proper management of natural regeneration and ex situ genotype collections might be a good conservation strategy to maintain G. crinita genetic resources.
The use of morphological (wood physical traits) and molecular markers were successful to reveal genetic variability of G. crinita and they could be used for other tropical tree species. For further researches it is emphasis to extent the number of samples and geographic scale.
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Economic Impacts of Social Policy on Selected Sectors of the Czech Economy
Kašparová, Kateřina ; Severová, Lucie (advisor)
We live in the 21st century, when more than ever solves finance, economy in terms of both the individual and the state, but also the European Union, which affects every Member State and the Czech Republic is no exception. State economic situation continues to affect both small and large companies, entrepreneurs and individuals who under the influence of the surrounding developments make decisions affecting not only the present but also the future. Economic decisions impinge on the functioning of the state authorities, which is taking steps to support the decision given on the company, such as electronic records of sales, taxation and other laws affecting their business. This impacts directly or indirectly to a particular individual moving in the country's economy. The aim of the dissertation is to express the economic impact in terms of social policy on selected sectors of the Czech economy, both theoretically and practically. The economic impact of social policy affects the financial situation of the economically active population who are hit by unexpected life situations. It is degraded in the social position due to declining economic level, which is then inadequate and social policies often do not sufficiently take into account the economic situation of the individual before and after the establishment of that situation. And the individual is often unable to adequately use the possibilities that social policy allows. The main reason is a lack of awareness, that should act on the individual before the emergence of the situation and create awareness in the people about the possibilities of resolving the situation. Unexpected life situations addressed by social policy, which is also solvable through its tools, job loss or inability to continue to engage in employment. Both options associated with a decrease in working capacity, which addresses social policy through the provision of disability pensions. These covers Czech Social Security Administration through district administrations throughout the country. The State influences the amount of disability pensions minimum the setting of wages, the basis of assessment, determination of the percentage amount of disability pension, including its reduction limits or minimum time required insurance. The aforementioned indicators in the calculation of invalidity pension, which becomes a very individual matter, but is influenced by the rules of 441,000 persons receiving this pension, which in the third. stage reaches an average amount of 10 262 CZK. With this funding slightly higher than the minimum wage, individuals must manage and deal with the new situation. Economic impacts caused by unexpected life situation affects not only the individual himself, but also its surroundings. To eliminate these negative economic impacts serve other instruments of social policy that could improve the financial situation. However, this is a significant administrative burden for which the required knowledge about the possibilities, which include care allowances, mobility allowances, certificate of persons with disabilities, but also the opportunity to appeal against the decision of the Czech Social Security Administration to withdraw and so improve its economic situation.
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Camera trap as a non-invasive tool in research on rare and elusive mammals
Jůnek, Tomáš ; Hejcmanová, Pavla (advisor)
Knowledge of the richness of species that inhabit a particular area is an essential metric for both conservationists and practitioners. Relatively cost-efficient and easily standardized solution has occurred in last the decades with the massive digitalization of photography, the camera trap. Such an independent detector is bias-free in the probability of recording objects passing by and in most cases does not disturb animals. Recent discoveries of new mammal species are proof of this. Moreover, camera traps allow for insight into the otherwise hidden behaviour of even the most timid animals. My interest in photography, endangered animal species and nature conservation has led me to the decision to apply a novel method in practice. Me and my team have focused on three different use-confirmation of the accuracy of capture-recapture population estimates, temporal overlaps of invasive species with its prey and competitors, and first ever camera-trapping species inventory in the Niokolo-Koba National Park. Thanks to personal experiences and contacts from World biodiversity hotspots I was able to successfully employ an approach and carry out field studies. I hope that our results will contribute to the development of effective conservation strategies and the methodology itself.
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Production yield of sorghum grown for biogas production
Adamčík, Jiří ; Pulkrábek, Josef (advisor) ; Vladimír, Vladimír (referee)
Low temperature during germination is a major limiting factor affecting growth of sugar sorghum in the Czech Republic. The aim of the study was to determine the ability of stimulated seeds germinate in slightly adverse conditions (less optimal temperatures) and evaluate the impact of biologically active compounds to faster germination of sorghum seeds and next plant growth. Two sorghum seed lots were treated by auxinous preparations Lexin and M Sunagreen. Germination was tested in germination chamber at temperatures 12, 15, 18 and 21 °C in the dark for 14 days. The results confirm stimulation of sugar sorghum seeds and increase of the germination speed at lower temperatures. Top acting biologically active agent (for faster germination) was Lexin. This product has a significant impact on increasing of the seed vigour. In less favorable conditions (12 ° C) application of Lexin increase germination speed of sorghum seeds by a full five days. Shortening of mean germination time was detected after M-Sunagreen application too. 25 cm row spacing on sorghum sugar and 50 cm on the grain sorghum were the best in our experiments. We have found that fertilizer Urea Stabil before sowing at a dose of 80 kg N.ha1 and then the same dose fertilization in stage BBCH 35 is preferred.
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