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Prevention of nosocomial infections origin and spreading in Český Krumlov a.s. hospital
KOCOURKOVÁ, Lenka
The present Bachelor thesis focuses primarily on the prevention of occurrence of hospital-acquired infections and the causes of spread of these infections across healthcare facilities. The thesis relies on active communication with the coordinator for management of quality and safety of nursing care, who helpfully provided me with valid information and documents relating to the system of hospital-acquired infection prevention, and arranged for sampling for microbiological examinations in postoperative care departments (ONP) - ONP II and ONP - C III, the hospital Nemocnice Český Krumlov a.s. The Bachelor thesis consists of a theoretical part and a practical part. The theoretical part summarizes knowledge related to nosocomial infections as such, the division and manner of transmission, with an emphasis of prevention of occurrence of these infections and spread of the same among "healthy hospitalized persons." The practical part explores the hospital's approved internal documents aimed at prevention of nosocomial infections and training of staff in the healthcare facility. Moreover, the practical part also verifies the prevention of nosocomial infections by means of microbiological tests. The entire research was carried out in cooperation with two hospitals: Nemocnice Český Krumlov a.s., i.e. the location of sampling, and Nemocnice a.s. Prachatice, the location of microbiological processing. The laboratory-based research part was solved in a microbiology laboratory in the hospital Nemocnice Prachatice a.s., with the aim of identifying the individual microorganisms from the smears taken in the postoperative care department in the hospital in Český Krumlov. These smears reflect the quality of the performed hygiene in connection with the risk of occurrence of nosocomial infections. In addition to laboratory-based research activities, the present thesis also examines the current quality and processing of the hospital's internal documents relating to the prevention of nosocomial infections and the training schedule for the hospital's staff in this respect - trainings are conducted on an annual basis. The thesis is concluded by a summary of isolated microorganisms and comparison of these microorganisms in the framework of the two hospital departments. Last but not least, the presence of highly infectious agents for occurrence of nosocomial infections is excluded on the basis of valid legislation. The thesis also provides a result of qualitative processing in a form of recommendations e.g. for improvement of purpose-directed cleaning. The thesis may therefore be used as a tool for improving awareness of this problem area.
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Attitudes of Czechia and Slovakia towards the forced sterilization of Roma women in comparative perspective
Slaninová, Lucie ; Klípa, Ondřej (advisor) ; Kolenovská, Daniela (referee)
The bachelor thesis is a comparative two-cases study that compares approaches of Czechia and Slovakia towards forced sterilizations of Roma women that was taking place on their territory during several decades. Sterilization is a medical intervention disabling a human reproduction. Sterilizations were set in the seventies in the former Czechoslovakia in accordance with an eugenics ideology of controlling public reproduction and influencing quality of the society. Ongoing differencies between major society and Roma people lasted for centuries in spite of many attempts to assimilate Roma people and that is the reason why Roma women were the target. However, controlling reproduction of population is violation of human rights. For this reason, both countries had to accept apropriate restrictions. The sterilization policy was ignored for a long time, although complaints and activities of victims started to appear at the beginning of this century. All the speaking victims together with many nongovernmental organizations tried to obtain a compensation for victims of this unlawful policy of forced sterilizations. Therefore, state authorities of both countries started to act. First of all, Slovakia started interrogation as a consequence of a report "Body and Soul" in 2003. A new act of health care providing...
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Transsexuality in law
Nguyenová, Nikola ; Šustek, Petr (advisor) ; Hendrychová, Michaela (referee)
Transsexuality in law Abstract Gender reassignment is a topical issue that concerns only a relatively small number of people and is not given the necessary attention in the Czech legal environment, although the legislation is insufficient for a long time and does not comply with internationally recognized human rights standards. The legal system is based on the binary concept of gender and trans people are often unable to place themselves within it. The title of the thesis reflects the legal situation of trans people in the Czech Republic, as the legislation pays attention only to transsexuals and allows them to achieve life in the official gender they identify as. The law forgets other trans people who, for various reasons, do not wish to undergo surgical gender reassignment and, due to unfavorable legislation, have to deal with unpleasant and humiliating situations and discrimination. The thesis provides an explanation of the terminology of the issue, a description of the process of gender reassignment and an analysis of the legal status of transgender people in the Czech Republic with an emphasis on a critical analysis of key aspects of gender reassignment. In addition, the provisions of the Czech legislation in the Civil Code, the Act on Specific Health Services, the Act on Registries and the Act on...
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Design of algorithms for a continuous steam quality measurement system
Hanák, Tomáš ; Tofel, Pavel (referee) ; Holcman, Vladimír (advisor)
This bachelor thesis deals with proposal of algorithm for continuous system of sterilizated steam. At the beginning we deal with the process of sterilization and its methods. Later on we talk over about the saturated steam and parameters of saturated steam. At the end of the bachelors thesis we discuss about current measurement of sterilization steam quality. Then, the procedure of simultaneous measurement of sterilization steam quality is described here. The last part is the evaluation of measured data and presentation of their results.
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Design of algorithms for a continuous steam quality measurement system
Hanák, Tomáš ; Tofel, Pavel (referee) ; Holcman, Vladimír (advisor)
This bachelor thesis deals with proposal of algorithm for continuous system of sterilizated steam. At the beginning we deal with the process of sterilization and its methods. Later on we talk over about the saturated steam and parameters of saturated steam. At the end of the bachelors thesis we discuss about current measurement of sterilization steam quality. Then, the procedure of simultaneous measurement of sterilization steam quality is described here. The last part is the evaluation of measured data and presentation of their results.
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Legal aspects of gender reassignment
Dočekalová, Hana ; Frinta, Ondřej (referee)
Legal aspects of gender reassignment Abstract Thesis deals with legal aspects of gender reassignment, especially with legal conditions of gender reassignment witch are required by the Czech law. Furthermore, the thesis provides a general introduction to the issue of transsexuality, deals with the etiology of transsexuality, and defines its two fundamental types, namely the MtF and the FtM transsexuality. This thesis also recalls the position of transgender people in society, as transgender people continuously face various forms of discrimination. In particular, the text of the thesis is focused on the legal analysis of the issue of gender reassignment. The thesis deals with all legal regulations that contain provisions concerning gender reassignment. In this manner, it deals with all relevant legal provisions included in the Czech Civil Code, in the Act on Specific Health Services, and in the Registry Act. The thesis also deals with the possible future legal regulation of gender reassignment using the de lege ferenda considerations. This bill could adapt the Czech legal regulation of gender reassignment to the case-law of The European Court of Human Rights as well as the legal standarts in other european countries. In the thesis, there is also the whole process of gender reassignment including both the...
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Legal aspects of gender reassignment
Dočekalová, Hana ; Hendrychová, Michaela (advisor) ; Šustek, Petr (referee)
Legal aspects of gender reassignment Abstract Thesis deals with legal aspects of gender reassignment, especially with legal conditions of gender reassignment witch are required by the Czech law. Furthermore, the thesis provides a general introduction to the issue of transsexuality, deals with the etiology of transsexuality, and defines its two fundamental types, namely the MtF and the FtM transsexuality. This thesis also recalls the position of transgender people in society, as transgender people continuously face various forms of discrimination. In particular, the text of the thesis is focused on the legal analysis of the issue of gender reassignment. The thesis deals with all legal regulations that contain provisions concerning gender reassignment. In this manner, it deals with all relevant legal provisions included in the Czech Civil Code, in the Act on Specific Health Services, and in the Registry Act. The thesis also deals with the possible future legal regulation of gender reassignment using the de lege ferenda considerations. This bill could adapt the Czech legal regulation of gender reassignment to the case-law of The European Court of Human Rights as well as the legal standarts in other european countries. In the thesis, there is also the whole process of gender reassignment including both the...
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Použití CRIPR/Cas9 a nové techniky značení zárodečných buněk pro náhradní reprodukci u jeseterů
KHANZAI BALOCH, Abdul Rasheed
Sturgeons are commonly known as living fossils or ancient giants that diverged from ancient pre-Jurassic teleost lineage approximately ~300 million years ago (Mya). Sturgeons' 85% species are listed as critically endangered in the International Union for Conservation of Nature (IUCN). Sturgeons' reproductive traits such as delay in sexual maturation and periodic interrupted spawning cycles make their rehabilitation more difficult. However, among sturgeon species, the sterlet (Acipenser ruthenus) has shortest sexual maturation period. Therefore, it can be used as a host in surrogate production in sturgeons. Dnd1 was discovered as germ-plasm specific maternal RNA that exclusively expresses in vertebrate germ-line. Various studies have confirmed that dnd1 protein is essential for Primordial Germ Cells (PGCs) migration; and disruption of the PGCs migration affects fish fertility. Dnd1 deficient PGCs in zebrafish transdifferentiate into somatic cells. Previously our colleagues used morpholino oligonucleotide to knock down dnd1 in sterlet to produce germ cell free host for surrogate production. CRISPR/Cas9, a cutting-edge genome editing technology is being used in different research fields; here we thus aimed to harness the power of aforementioned technology to knock out dnd1 in sterlet. No or less number of PGCs were observed in CRISPR/Cas9 injected embryos as compared to control group injected with FITC-dextran only in order to label PGCs. Furthermore, we compared three different sterilization techniques viz., CRISPR/Cas9 and morpholino oligonucleotide (MO) targeting dnd1 and ultraviolet irradiation to eliminate PGCs in sterlet. Our data showed higher hatching and survival rates in CRISPR/Cas9, UV irradiation, and MO knockdown groups, respectively. Interestingly, some embryos treated with CRISPR/Cas9 displayed malformations. We presume that malformations were due to off-target effects and/or due to double injections i.e., injection of CRISPR/Cas9 at animal pole to knock-out the dnd1 and FITC-dextran at vegetal pole. Taking advantages of Iron Oxide nanoparticles (IONs) applications in various burgeoning research fields, we opted to use them to label PGCs in sturgeons. We injected IONs combined with FITC-dextran into vegetal pole of sturgeon embryos, and have successfully labelled the PGCs. Injection of IONs in sturgeons did not affect hatching and survival rates of embryos. Interestingly at 5 dpf, significantly less number of FITC-dextran labelled PGCs in FITC-dextran/IONs injected group were observed when compared with PGCs that were labelled with FITC-dextran only. Less number of PGCs in IONs injected group presumably could be because of interference posed by IONs to PGCs during the course of their migration. This is first study of its kind where germ cells of any species have been labelled by using nanoparticles. In conclusions, this thesis provides information regarding role of Dnd1 protein as potential germ-cell molecular marker in various fish species, and its use for conservation of fish species. Dnd1 knockout sterlet can be potentially used as sterile host for surrogate production in sturgeons. Moreover, labelling of PGCs in sturgeons by using IONs can thus open new avenues to study interactions of nanoparticles with cells that will ultimately help in hyperthermia where cells/tissues are exposed to electromagnetic field increasing temperatures to activate their death. After insertion of IONs to PGC in sturgeon embryo, it could be possible to isolate PGC using a magnetic field or to apply hyperthermia for host sterilization purpose.
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Study of chemical processes initiated by electrical discharge in liquids
Možíšová, Aneta ; Zlámalová Gargošová, Helena (referee) ; Kozáková, Zdenka (advisor)
This Bachelor´s thesis in the first part describes the process of ignition of the plasma discharge in water solutions, the generation of the particles that are generated during the discharge and their subsequent analysis by appropriate analytical methods. The second part focuses on specific cases of the use of the plasma discharge in the environment of selected electrolyte, phosphates were selected for individual experiments and for the comparison of sodium chloride. Phosphates were chosen mainly because of their stable pH as a possible benefit in discharge. The aim is to obtain the most ideal environment for the ignition of the plasma discharge and to optimize the method, which could subsequently be used for medical purposes, especially when sterilizing tools
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