|
|
Modeling two-phase bubble flow in microfluidics
Stehlík, Martin ; Kozák, Jiří (referee) ; Rudolf, Pavel (advisor)
The goal of submitted thesis is to perform a computer simulation of bubble creation in T-channel. In the first section of the paper, the theoretical applications of microfluidic bubble, micromachines and droplet formation are described. In the second part of the text, author uses cross flowing method for simulation od bubble creation. Furthermore, several settings in computer simulation software Fluent are mentioned. In addition, the influence of velocity at the T-channel inlet on surface tension and on bubble length is presented.
|
|
|
Microfluidics
Abrahám, Martin ; Sluše, Jan (referee) ; Rudolf, Pavel (advisor)
The bachelor's thesis deals with phenomenon of gas and fluid flow in equipment with micro scale. The first part is dedicated to describe Reynolds number and laminar flow in micro channel. The second part is dedicated to describe specific equipment for pumping a working material – micropumps, microvalves, microneedels, micromixers.
|
|
|
Design of microfluidic mixer
Abrahám, Martin ; Fialová, Simona (referee) ; Rudolf, Pavel (advisor)
Microfluidic devices are more frequently being used in medicine as they operate with small amounts of test samples, such as blood or reagent chemicals. To work with such substances, effective mixing of the solution is usually required, which emerged as the most challenging problem in microfluidic systems. Due to the minor dimensions of the devices only laminar flow occurs, thus the turbulent eddies do not contribute to the mixing, but only the molecular diffusivity.
|
|
|
Microfluidic Enzymatic Reactor for Drug Screening
Königsmarková, Kristýna ; Peš,, Ondřej (referee) ; Přikryl,, Jan (advisor)
This master thesis deals with the use of microfluidics for the purpose of microfluidic enzymatic reactor for drug screening. At first it considers the issue from a theoretical point of view – describes microfluidics as a newly developing and promising field of production of microfluidic devices, materials, biomedical applications and advantages and disadvantages of microfluidics overall. Furthermore, it focuses on an area of analytical utilization of enzymes within enzyme reactors. In the first part of the experimental section, conditions for the testing of enzymes of xenobiotics metabolism in the liver were optimized, namely the model of coumarin metabolism via the spectrofluorimetry method. The second part of the experimental work dealt with optimization of the fabrication conditions of microfluidic chips from OSTE (off-stoichiometry Thiol Ene) via the soft lithography method. Subsequently, the functionality of the produced chips was tested. Based on the results of both parts of the experimental work, an evaluation was carried out to assess the suitability of their interconnection for future research – screening of microsomal enzyme activity and model biotransformation of drugs within the channels of the fabricated devices.
|
|
|
Surface acoustic wave microfluidic chip for rapid cell sorting
Vejrosta, Jakub ; Maňka, Tadeáš ; Plichta, Tomáš ; Šerý, Mojmír
This work revolves around microfluidics and micro-objects manipulation in general. In our device, we are producing surface acoustic wave (SAW). The SAW can be used to sort particles by their size or physical properties and separate them. In medical analysis SAW on microfluidic chip can be used to pre-sorting of body fluids or blood serum for further optical or chemical analysis. The wave is produced by pair of interdigital transducers (IDTs), which are situated on opposite sides of microfluidic channel. As our active material we have used lithium niobate crystalline material for its great piezoelectric properties. This work is also the precursor for measuring the rheological properties of a medium, such as micro-viscosity, via the measurement of the trajectory of a flow trace.
|
|
|
Dissolved gas effect on hydrodynamic cavitation
Piňos, Ondřej ; Fialová, Simona (referee) ; Rudolf, Pavel (advisor)
Thesis is focused on hydrodynamic cavitation in gas saturated liquid flowing in microfluidic circuit with Ventruri noozle. Thesis describes theoretical base in fields of: gas saturation of liquids, cavitation inception, diffusion mechanisms and cavitation collapses. In practical part experiment on microfluidic circuit was done. In pressurised tank liquid was saturated by gas, after letting the mixture into noozle we observed beginning of cavitation by acoustic and visual way. Inception of cavitation was also observed by -\sigma graphs. With increasing pressure in tank we observed developed cavitation, its persistivity was compared with increasing amount of gas in liquid. Measured values are supported by theoretical part of thesis. There was nonconformity in comparison of demineralised water and tap water saturated by air, where demineralised water cavitated during higher cavitation numbers for saturating pressures of 4 and 6 kPa. Further research could focus on undersaturated water as well as on better control of water properties, especially on number of nuclei within.
|
|
|
Directed evolution of enzymes on microfluidic chip
Kohúteková, Táňa ; Chmelíková, Larisa (referee) ; Prokop,, Zbyněk (advisor)
Tato práce se zaměřuje na řízenou evoluci enzymů pomocí fluorescenčně řízeného kapénkového třídení (ang. zkratka FADS) na mikrofluidním čipu. Byl sestaven nový třídicí systém, který byl validován pro vysoce výkonnou optickou detekci mikrofluidních kapének spojenou s dielektrickým tříděním. Systém byl testován pomocí dvou různých (DhaA31, ancHLD-RLuc) populací haloalkan dehalogenas (HLD) exprimovaných v buňkách Escherichia coli a jedné populace prázdného vektoru pIDR9 v poměru 5:45:50, což imituje proteinovou knihovnu. Naměřená a analyzovaná data z experimentu ukazují, že intenzity kapek ve validačním experimentu byly zaznamenány v stejném poměru jako teoretický poměr. Poté byla knihovna HLD mutantů s inzercemi a delecemi tříděna pomocí FADS, za účelem získaní 1 % nejaktivnějších variant. Program pro analýzu dat pro FADS byl programován v prostředí LabVIEW s využítím jazyka Python, s funkcemi pro počáteční analýzu, predikci prahového napětí, validaci a grafickou interpretaci dat a konečnou zprávu o experimentu.
|
|
|
A microfluidic platform for working with biological material
Vírostko, Ján ; Švarc, Vojtěch (referee) ; Hrdý, Radim (advisor)
Táto bakalárska práca sa zameriava na návrh a výrobu mikrofluidej platformy určenej pre lýzu buniek a ich následnú analýzu. Táto platforma by mala byť schopná efektívne mechanicky rezať bunky v preparátoch červených krviniek a následne ich analyzovať pomocou dvojelektródového elektrochemického systému. Teoretická časť tejto práce je zameraná na technológiu laboratória na čipe a používané komponenty v tejto technológií, ako napríklad mikrofluidné separátory, mixéry, komory určené na lýzu alebo mikroreaktory, ich aplikáciu a na koniec ich výrobu v čistých priestoroch. Taktiež je časť venovaná stručnej rozprave o fyzike prúdenia tekutín v mikroskopických kanáloch. Praktická časť tejto práce sa skladá z návrhu, výroby v čistých priestoroch a zapúzdrenia troch typov spomínanej platformy pomocou polymeru Parylene C. Následne je popísaná príprava zariadení na testovanie a ich následne testovanie. Na záver je uvedená dokumentácia výroby pomocou vytvárania snímkov s použitím elektrónového mikroskopu a grafické závislosti vypočítané počas charakterizácie elektród a snímky správania sa analytu v komore na lýzu.
|
|
|
Proceedings of APCE-CECE-ITP-IUPAC 2022
Foret, František ; Lavická, Jana ; Chung, D. S. ; Drobníková, Iveta ; Přikryl, Jan ; Lee, H.
The joint International Interdisciplinary Conference of Chemical Analysis, APCE-CECE-ITP-IUPAC 2022, comprising the 18th Asia Pacific International Symposium on Microscale Separations and Analyses, the 17th International Interdisciplinary Meeting on Bioanalysis, the 28th International Symposium on Electro- and Liquid Phase-Separation Techniques, and IUPAC Special Symposia by Division of Chemistry and the Environment, provided full texts of 108 oral and 54 poster presentations. The attached conference proceedings, edited by highly cited scientists, document the quality of the meeting. Similarly to previous years, the lectures were given by distinguished scientists. The list of speakers as well as more detailed information about the conference can be found on its permanent web at www.ce-ce.org or in the proceedings published in Separations (https://doi.org/10.3390/separations10020109).
|
|
| |
guest ::
