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A microfluidic platform for working with biological material
Vírostko, Ján ; Švarc, Vojtěch (oponent) ; Hrdý, Radim (vedoucí práce)
This bachelor's thesis focuses on the designing and fabrication of a microfluidic platform for cell lysis and subsequent analysis. This platform should be able to mechanically lyse cells in red blood suspensions efficiently and analyse the product using a two-electrode electrochemical system. The theoretical part of this thesis is dedicated to lab-on-a-chip technology and its components, such as microfluidic separators, mixers, lysis chambers and micro-reactors, their application and finally, their fabrication in clean rooms, along with basic physics of fluids in microchannels. The practical part of the thesis consists of designing three variants of the mentioned microfluidic platform, its fabrication in clean rooms and encapsulation using Parylene C polymer. The thesis follows the preparation of fabricated devices for testing and testing itself. The thesis concludes with imaging using a scanning electron microscope as a documentation of the fabrication process, resulting graphs from an electrode characterisation and images of cell behaviour within the lysis chamber.
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