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Coordination properties of NOTA monoamide
Kubinec, Jan ; Kubíček, Vojtěch (advisor) ; Matulková, Irena (referee)
The aim of this thesis was to prepare monoamide of macrocycle NOTA, which was successfully prepared and fully characterized. Acid-base properties were investigated by potentiometric titration and four protonation constants were found, which are lower than pKa ` s of NOTA ligand. Coordination properties with selected ions from the first row of transition metals were investigated. Stability constants of studied complexes are also lower than the same complexes with NOTA ligand. Key words: macrocyclic complexes, positron emission tomography, thermodynamic stability, formation kinetic, radiopharmaceuticals
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Study of plasma protein binding of radiopharmaceuticals
Hafinec, Václav Matyáš ; Lázníček, Milan (advisor) ; Ramos Mandíková, Jana (referee)
Study of Plasma Protein Binding of Radiopharmaceuticals Summary The purpose of this work is the study of binding of substances (177 Lu-DOTA- [Lys3]bombesin, 177 Lu-NOTA-[Lys3]bombesin, 177 Lu-PCTA-[Lys3]bombesin, and 177 Lu- DOTA-MG47) to plasma proteins by equilibrum dialysis in 37řC, particularly using plasma samples of beef, rabbit, rat and human. Within this group, these substances were compared interspecifically. The substances 177 Lu-DOTA-[Lys3]bombesin, 177 Lu-NOTA-[Lys3]bombesin, 177 Lu- PCTA-[Lys3]bombesin, and 177 Lu-DOTA-MG47 are the newly developed receptor- specific radiolabeled peptides. For all the newly collected data, the interspecific comparison and subsequent statistical evaluation was performed. The indicated bombesin derivates were compared and statistically analyzed even between themselves. During the interspecies comparisons and the determination of the statistical significance of the data, there were found statistically significant and statistically highly significant differences between some of the examined samples. A highly significant difference was found during comparing with samples of 177 Lu-NOTA- [Lys3]bombesin and statistical evaluation, there was found a statistically highly significant difference. Despite the differences found, it is clear that the plasma binding concerning...
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Study of interspecies differences in plasma protein binding of 18F-labeled radiopharmaceuticals
Nováková, Dana ; Lázníček, Milan (advisor) ; Bárta, Pavel (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Mgr. Dana Nováková Consultant: Prof. PharmDr. Ing. Milan Lázníček, CSc. Title of Thesis: Study of interspecies differences in plasma protein binding of 18 F-labeled radiopharmaceuticals In this thesis, the binding ability of three radiopharmaceuticals containing radionuclide 18 F to the plasma proteins was examined in bovine, pig, human and rat plasma, and the interspecies comparison was performed. Examined radiopharmaceuticals included 18 F-FDG, 18 F-fluorocholine and 18 F-thymidine. The two main methods used are equilibrium dialysis and ultrafiltration. These methods are based on separation of free ligand from the linked component. During the separation, semi-permeable membrane and special filters (AMICON and VIVASPIN) were used for equilibrium dialysis and ultrafiltration, respectively. Free fraction of drugs is considered to be an important parameter for the availability of the drug distribution into the tissues and cells, interaction with receptors, excretion and metabolism. All experiments were carried out at the physiological temperature of 37řC. Obtained results indicate low plasma protein binding of the three radiopharmaceuticals in all studied species, which suggests...
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A patient after a diagnostic application of radiopharmaceuticals as a source of radiation - mutual radiation strain of the personnel and patients
MARKOVÁ, Iveta
ABSTRACT A patient after a diagnostic application of radiopharmaceuticals as a source of radiation - mutual radiation strain of the personnel and patients My work focuses on the radiation protection on account of the potential influence of ionizing radiation with its negative effects on a human organism. In my work I concentrated on observing the obtained personal equivalent doses of the ionizing radiation in the Department of Nuclear Medicine in the Hospital of České Budějovice, plc. I also focused on the measurement and assessment of the dose rate obtained by the employees of the Department of Nuclear Medicine in the Hospital of České Budějovice, plc. from treated patients with regard to the distance and type of the applied radiopharmaceutical. The methods chosen for the actual measuring, gathering, recording and assessment of the data are described in a great detail and presented in the methodology of my work. The data obtained from the personal TL dosimeters is registered and filed in the Department of Nuclear Medicine in the Hospital of České Budějovice, plc. on a long term basis. Therefore it enabled me to analyze the equivalent dose of four nurses during the last five years. The results were compared with the figures determined in the regulation 307/2002 of the Code, as amended by regulation 499/2005 of the Code. The results are then clearly presented in tables and graphs. The collected data has confirmed that the values of equivalent doses obtained from the personal TL dosimeters of four nurses working in the Department of Nuclear Medicine in the Hospital of České Budějovice, plc. do not exceed figures determined by the regulation 307/2002 of the Code, as amended by regulation 499/2005 of the Code. The measured dose rates decrease with the distance and are proportional to the activity and type of radiopharmaceutical.
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Radioactive waste management in the department of nuclear medicine
VENCLÍK, Zdeněk
Radioactive waste management in the department of nuclear medicine is a complex process in which it is necessary to obey a number of lawful orders. These are based on physical and chemical properties of the radionuclide sources. At present these regulations particularly include Act No. 18/1997 Coll., on Peaceful utilisation of nuclear energy and ionizing radiation (the Atomic Act) and amendment to and alteration of some related acts in the wording and implementing Decree No. 307/2002 Coll., on Radiation Protection (which repeals Decree No. 184/1997 Coll.) in the wording of Decree No. 499/2005 Coll., which amends the Decree of the Czech National Council for nuclear safety No. 307/2002 Coll., on Radiation Protection. This legislation regulates the management of radioactive waste as a whole and deals with specific radionuclides in the annexes. It is necessary to comply with legislation whether the radioactive waste is generated in the actual production of radionuclides or during their diagnostic and therapeutic applications. All production and application processes give rise to certain materials contaminated with radionuclide sources but without the possibility of their further use. These contaminated and unusable materials are called radioactive waste. Radioactive waste arising from operation of nuclear medicine is basically divided into solid, liquid and gaseous. Solid radioactive waste is collected in refrigerators and lead safe deposits, depending on whether the waste is infectious or not. Liquid waste goes through the process of dilution in most cases, when there is a decrease of the volume activity limits and subsequent discharge to sewer. Gaseous waste is a negligible component of radioactive waste produced at nuclear medicine departments, so it is not often dealt with in more detail. No matter what kind of waste, the process of its disposal has a common goal of reducing its activity below specified limits (clearance levels), so that the waste can be without greater risk moved or released and disposed of as other, non-radioactive waste.
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