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Anti-aging cosmetics - latest activ substances of natural origin
Fleková, Andrea ; Vítová, Eva (referee) ; Souralová Popelková, Miriam (advisor)
The present diploma thesis is focused on the selected anti-aging substances in cosmetic products. The literature research provided us with the knowledge of essential skin characteristics and factors influencing the aging process. Furthermore, the thesis describes the basic physical forms of cosmetic products, the basic cosmetic materials and the current trends in the usage of anti-aging substances. The experimental part subsumes altogether four physical forms of cosmetic products – tonics, serums, masks and creams. In this work was testid a new anti-aging compound, developer by French company SOLABIA, which provided the company Miša a Harašta s.r.o. (M+H). It has tested four components: Fucogel, Camaderm Gly, Viniderm and Omega Ceramid. These anti-aging substances were addend to the four technology cosmetic form, which were prepared in our laboratory. Subsequently, the pH stability of the products was measured together with the rheological measuring and skin hydration measuring. All of the samples proved to be pH stable. The highest stability of viscosity and consistence was identified for masks and creams. It was found out that the added anti-aging substances have no impact on the stability of pH, viscosity and consistency. The skin hydration measuring proved that the anti-aging substances Vinidem and Camadem Gly have the greatest moisturising qualities.
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Determination of alergenic and potential alergenic metals in cosmetics
Krakovková, Lenka ; Zemanová, Jana (referee) ; Diviš, Pavel (advisor)
The aim of the diploma thesis was to provide an overview of the prevalence of allergenic and potentially allergenic metals in eye shadows. The diploma thesis gives an overview of the legislation on cosmetics and description of the types of allergenic reactions caused by allergenic and potential allergenic metals in eye shadows. Listed below are the preparation methods of the samples for analysis and the list of the methods by which can the selected metals be analyzed. The experimental part of the diploma thesis deals with an analysis of selected allergenic and potentially allergenic metals in eye shadows. In the experimental part of diploma thesis method of sample preparation for analysis of eye shadows and a method of analysis of sample of eye shadows by ICP-MS have been optimized. Monitored analytes were selected metals. Altogether 6 samples of eye shadows from different manufacturers were chosen. Measured results have been statistically processed, confronted with the applicable legislation and assessed in terms of possible allergic reactions.
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Fragrance Allergens in Foods and Everyday Use Products
Divišová, Radka ; Buňka, František (referee) ; Hojerova,, Jarmila (referee) ; Omelková, Jiřina (advisor)
Fragrances are added to food, cosmetics and other products primarily for enhancement of their sensory quality (taste and/or aroma). However, the usage of these compounds is associated with the wide range of various adverse biological effects. Some fragrances widely used in cosmetics are proved to cause e.g. skin sensitization, rashes, dermatitis, headache, cough etc. To protect the health of consumers, European union approved the cosmetic directive (ES/1223/2009) that requires the labeling of 26 allergen fragrances on the final product label, if concentration exceeds the given level (0,01 % for rinse-off and 0,001 % for leave-on product). However, some producers hide the presence of allergens in the final product under the general term „aroma“. For this reason it is very important to monitor the content of these substances in cosmetic products. Fragrances are found not only in cosmetics, but the various types of food are also aromatized by them. Unlike cosmetics, the food producers are not obliged to label the presence of fragrance allergens on the packaging. Therefore, the monitoring of these fragrances is highly desirable because of the potential health risks they pose. The aim of this study was to develop a method for simultaneous determination of regulated fragrance allergens in food and everyday use products. The method based on extraction of analytes by solid phase microextraction coupled to gas chromatography with FID detection (HS-SPME-GC-FID) was chosen on the basis of the literature review. The fiber CAR/PDMS provided the highest extraction efficiency among the SPME fibers tested. Univariate and multivariate data analysis were used to optimize the main parameters affecting microextraction process. The final method validation was performed in terms of linearity, repeatability, reproducibility, recovery, limits of detection and quantification. The optimized and validated method was applied to a wide range of products including cosmetics, aromatized food and fragranced toys. These products were also subjected to sensory evaluation especially in terms of taste and/or aroma (i.e. flavour), which may be associated with the content of the monitored fragrances. The profile test (EN ISO 13299) and seven-point category ordinal scale (ISO 4121) were used for sensory evaluation.
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Use of antimicrobial products of animal origin in cosmetics
Puškárová, Radka ; Bokrová, Jitka (referee) ; Márová, Ivana (advisor)
The Bachelor thesis deals with characterization a whey in free and encapsulated form and its application to cosmetic industry. The theoretical part is focused on characterization of whey, especially on its antimicrobial properties, and use of this effect in selected cosmetics preparatives. Further, methods of encapsulation and particles characterization were described. In the experimental part, antimicrobial properties of whey were tested by two different methods. Whey was encapsulated into three different types of particles: liposomes, alginate and chitosan. Long-term colloid stability of particles during one and three weeks of storage in model conditions was tested. Further, particles were added into model skin creams. Long-term sedimentation stability of these emulsions was analyzed by analytical centrifuge.
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Sales Promotion and Advertising of Company
Liška, Marek ; Bednářová, Markéta (referee) ; Mráček, Pavel (advisor)
The main goal of my work is based on the analysis to propose appropriate changes / additions to marketing communication tools of a particular company, focusing mainly on sales promotion tools and advertising. The implementation of the proposals should then increase the awareness of the company, gain new customers or increase the satisfaction (and thus re-visit) of existing customers. I also want to focus on the start-up and subsequent operation of the company in connection with the pandemic situation and the resulting crisis. The first theoretical part is focused on defining individual economic concepts, methods and tools of communication. The second practical part is mainly based on the method of analysis of the current state of the company and the competition. In this part, I also focus mainly on the communication tools used by the salon, on the target group of customers, and the overall evaluation.
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Assessment of chosen aromatic compounds in cosmetics
Novotná, Petra ; Souralová Popelková, Miriam (referee) ; Vítová, Eva (advisor)
This master thesis deals with the assessment of selected fragrant substances in cosmetic products. At the beginning the occurrence, methods of acquiring and application of these substances is described. They are essential components primarily in cosmetic products, however they can cause an allergic reaction in sensitive individuals. The theoretical part of this thesis also describes the cause and the progress of these undesirable effects. There are several hundreds, up to thousands fragrant substances used in the perfume industry, from which 26 was designated by the European regulations as potential allergenic fragrant substances and their content is regulated. Legislative standards as well as the overview of the methods used to determine these fragrances are given here. The experimental part is focused on the development and validation of the separation methods for determining of the selected and potentially allergenic fragrant substances. The method used was a solid phase microextraction using Carboxen/Polydimethylsiloxane fiber combined with a gas chromatography coupled to a flame ionization detector (SPME-GC-FID). Amylcinnamyl alcohol, benzyl alcohol, cinnamyl alcohol, coumarin, lyral, ?-isomethyl ionone were among the determined allergenic substance. Consequently, this method was also applied on 10 selected cosmetic products specimens, in which the linalool and geraniol were identified as the most common allergenic substances.
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Humic acids in medicine and cosmetics
Šmídová, Helena ; Sýkorová,, Ivana (referee) ; Pekař, Miloslav (advisor)
The main concern of this study was to form a web and literature search focused on the known theoretical knowledge, patent application and the actual realization, for the use of humic acids or material containing humic acids in human and veterinary medicine and cosmetics. The output is also a proposal to be promising directions in exploring these areas. The introduction deals with humic substances in general with emphasis on humic acids, history of humic chemistry and structure of humic substances. The second part deals with medical aspects, pharmacological effects and the use of humic substances in human medicine, veterinary medicine and cosmetics, also takes account of humic substances from the perspective of the environment. The task of third experimental part was to learn the basics of isolation and characterization of humic acids (measurement of IR and UV / VIS spectra and determination of moisture and ash in samples of humic acids).
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Less conventional botanical sources of cosmetic raw materials
Novotná, Petra ; Vespalcová, Milena (referee) ; Zemanová, Jana (advisor)
The aim of this bachelor’s thesis is an investigation of use of less traditional plant sources in cosmetic industry. This review is focused on black elder (Sambucus nigra), sea buckthorn (Hippophae rhamnoides), quince (Cydonia oblonga), dog rose (Rosa canina), and stinging nettle (Urtica dioica). The substances Isolated from these plants can be used in various cosmetic products. The first section of this search deals with history and botanical characterization of these plants. The next section deals with significant substances contained in these plants and their use not only in cosmetic industry.
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Preparation of encapsulated enzymes for cosmetics application
Bokrová, Jitka ; Čarnecká, Martina (referee) ; Márová, Ivana (advisor)
Presented diploma thesis is focused on testing of an appropriate form of encapsulated enzymes intended for application in cosmetic and pharmaceutical industry. For encapsulation, proteolytic enzymes bromelain, papain and collagenase were used. These enzymes were encapsulated into alginate and chitosan microparticles prepared by an encapsulator and packed into liposomes. Encapsulation effectiveness was evaluated by analysis of total proteins. Particles stability was evaluated in model and real conditions by photometrical analysis of released proteins. Proteolytic activity of released enzymes in model and real conditions were observed too. Alginate and chitosan microparticles prepared by the encapsulator were found as an appropriate form of encapsulated enzymes designed to wound healing. Encapsulation effectiveness of these particles and stability in model conditions were good in comparison with liposomes. Hydrogel and water-oil emulsion were used for analysis of particles stability at real conditions. Hydrogel was found as a good option for preservation of particles as well as proteolytic enzyme activity. Emulsion made particles less stable and proteolytic activity of enzymes decreased rapidly. Encapsulation enables long-term stabilization of biologically active compounds as well as possibility of targeted transport and controlled releasing. Presented diploma thesis suggests possibilities of application encapsulated enzymes in designing more effective formulations for wound healing.
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Preparation of organic fibers with the addition of algae extracts
Tuhrinská, Terézia ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
The presented bachelor thesis is focused on the preparation and characterization of organic fibers with an admixture of pre-prepared extracts of microalgae and cyanobacteria containing valuable active compounds. The theoretical part describes selected metabolites of microalgae and cyanobacteria, methods of their analysis, the issue of encapsulation and incorporation of active substances into nanofibers and presents some methods of their preparation. In the experimental part of this thesis, selected strains from the CCALA collection were first cultured. Subsequently, aqueous, ethanol and hexane extracts of biomass from cultured and commercial microalgae and cyanobacteria were prepared. The prepared extracts were spectrophotometrically evaluated in terms of the concentration of chlorophylls, carotenoids, antioxidants, polyphenols, and total proteins present. In addition, 96 % ethanol extracts were subjected to more accurate analysis of chlorophylls and carotenoids by HPLC. The highest antioxidant activity was observed for aqueous extracts. Gelatin nanofibers containing selected extracts were formed by electrospinning method. The formed fibers were examined afterwards to identify a mass fraction of incorporated microalgal and cyanobacterial substances. The fibers were further tested for antioxidant activity with the TEAC assay. The most pronounced antioxidant effect was detected for the fiber with incorporated aqueous extract of the cyanobacterium Arthrospira maxima. Finally, the fibers were tested for their safety in contact with human cells. The amount of fibers used did not show any cytotoxic effect on human keratinocytes and the tested materials can thus be considered safe for application in cosmetics.
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