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Use of algae for production of biomass and bioproducts
Slonek, Jaroslav ; Máša, Vítězslav (referee) ; Brummer, Vladimír (advisor)
Submitted bachelor thesis is focused on description of use of algae for biomass and bioproducts production. The first part of the thesis is devoted to the taxonomic classification of algae and a basic summary of the characteristics of the different algal groups. The next part is aimed at bioproducts, which are obtained by algae cultivation and possibilities of further processing for energy purposes are also described. As for the cultivation, the emphasis is on modern methods in this area, i.e. on the possibility of using waste products in the culture process. In the next part, technological parameters of cultivation are discussed, for instance effects of technological parameters on growth and composition of biomass and production of desired products. The next chapter summarizes types of used culture bioreactors in which the growth of biomass occurs in a controlled environment. The information obtained and collected in the thesis is further discussed and summarized in the conclusion.
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CO2 reduction with algae
Naider, Jakub ; Rebej, Miroslav (referee) ; Vondál, Jiří (advisor)
The aim of this final thesis is the process of algae cultivation their use for carbon dioxid reduction. The main objective of this work is the mass and heat balance of the photobioreactor and the design of a temperature control system. Within the research work, an overview of certain types of bioreactors and photobioreactors, the current situation around the climate and the impact of the cultivation of algae on carbon dioxide emissions. The model for temperature control and mass transfer of a plate photobioreactor is created in this work.
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Spectrophotometric determination of phosphate in ternary systems with dyes
Novotný, Radek ; Komendová, Renata (referee) ; Sommer, Lumír (advisor)
The theoretical part of this master’s thesis is focused on the characteristics and forms of phosphorus, occurrence in natural waters and the problems associated with higher phosphorus concentrations, especially in the development of cyanobacteria and algae. There are also described the possibilities of determining the degree of pollution of natural waters by nutrients and the effects of eutrophication. There are also described methods for the determination of phosphates, especially spectrophotometric determination in ternary systems with dyes. Like other methods are mentioned AAS, ion chromatography, electrophoresis and izotachophoresis, electrochemical methods or enzymatic methods. In the experimental part of this work deals with the selection and optimalization of suitable ternary system with organic dyes for spectrophotometric determination of orthophosphates. The system with phosphovanadomolybdic acid is studied too. For analytical procedure with malachite green oxalate, which was found to be most useful, were studied interfering ions, commonly occurring in natural waters . The chosen procedure was finally tested on real water samples.
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A comparison of metabolic activity and use of microalgae and pigment-formed yeasts
Szotkowski, Martin ; Kočí, Radka (referee) ; Márová, Ivana (advisor)
Carotenoids are natural pigments occurring in plants and many microorganisms, such as algae, yeast and bacteria. They represent the most common group of antioxidants with significant biological effect. Lipids are an essential component of all living organisms. They are the source and the reservoir of energy for organisms, which can also be used in the petrochemical industry for the production of biofuels. This thesis deals with the production properties of carotenoids and lipids by selected strains of yeasts, algae, and canobacteria, in order to apply the acquired knowledge and find cheap suitable alternatives for microbial biotechnological production of these metabolites using waste substrates. Carotenoids, coenzyme Q, and ergosterol in cells were determined by liquid chromatography. The lipid content and fatty acid profile was analyzed by gas chromatography. Cell morphology and localization of selected metabolites were analyzed by fluorescence lifetime imaging microscopy. In this work yeast genera Sporobolomyces, Cystofilobasidium and Rhodotorula were used. As the representatives of the algae and cyanobacteria strains of Desmodesmus, Cyanothece, Chlamydomonas, Synechoccocus and Chlorella strains were studied. In the overall comparison, the yeast strains were more productive than algae and cyanobacteria. The highest carotenoid production was found in S. pararoseus, C. capitatum and R. mucilaginosa cells, while the highest lipid yield was observed in strains of C. infirmominiatum and S. metaroseus.
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Study of moisture conditions in the facade wall after application of the ETICS
Ševčíků, Robin ; Steuer,, Radek (referee) ; Šťastník, Stanislav (advisor)
In the last decades the application of ETICS (external thermal insulation composite system) became a popular measure to improve the energy performance and the weather resistance of facades in the building stock. However, hand in hand with that the problem of microorganisms such as algae and fungi on facades has substantially increased in re-cent years. One of the main reasons is the interaction of climate influences with modern compositions of thermal insulation systems resulting besides of large thermal and mois-ture loads in occurrences of green covers of algae on facade surfaces. The algae popula-tion on facades with ETICS is supported by the sufficient humidity, caused by the night temperature decrease of surface to the dew-point temperature and by consequent con-densation of vapour. The thermal radiation against clear night sky is responsible for the origin of this periodically repeated phenomenon. This bachelor’s thesis is concentrated to the description of available knowledge in this field and explains the phenomenons by means of principles of building physics. Moreover, at the end, in the practical part, there are presented the results of author’s own measurements concerning determination of basic properties of commonly used fa-cade materials (spectral emissivity, absorbing power), important with regard to the sub-ject of the thesis.
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New generation of biofuels
Pražák, Vojtěch ; Lisá, Hana (referee) ; Baláš, Marek (advisor)
This bachelor's thesis deals with different generations of biofuels. Their advantages and disadvantages and problems associated with their use in practice. The first part of the theses focuses on legislation and acquaintance with the issue of biofuels as a whole. The second part is focused on acquaintance and description of individual biofuels with examples of use in practice. At the end of the work there is an analysis of individual generations, representation in the fuel market and their efficiency.
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The issue of biotic attack of ETICS
Těžká, Adéla ; Žák,, Antonín (referee) ; Žižková, Nikol (advisor)
This bachelor thesis deals with the issue of biotic attack of ETICS contact insulation systems. The theoretical part is focused on the individual components of ETICS, both from the point of view of materials and from the point of view of individual steps. In addition, there are organisms that cause biotic attack on facades. The practical part deals with the possibilities of testing the resistance of the surface treatment of ETICS against biotic attack, both in laboratory conditions and in the exterior.
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Usage of flue gas as a source of CO2 for algae cultivation in bioreactors
Slonek, Jaroslav ; Juřena, Tomáš (referee) ; Brummer, Vladimír (advisor)
This master`s thesis is focused on possibility of use of flue gas as source of CO2 for algae cultivation in bioreactors. The first part of the master`s thesis is devoted to a qualitative and quantitative description of flue gas sources as CO2 source for algae cultivation. In the thesis industry sectors with the largest share of CO2 emissions on a global scale and in the Czech republic are summarized. On the basis of these data it was possible to determine cheap sources of CO2 for algae cultivation producing valuable biomass and other bio-products. The composition of flue gas from these sources was discussed in this section also with given expected outlook within the following years. The next part of the thesis is devoted to the influence of flue gas properties on algae cultivation. The impact of flue gas individual parameters on cell growth rate, quality and composition of biomass is described there. The description and design of the bioreactor is processed in the next part of the thesis. The experimental part of the thesis was performed on a bioreactor with similar properties. The next part of the thesis describes the effect of light on algae cultivation and the design of a regulation scheme for bioreactor light source. The experimental part of the thesis describes methods used to determine the concentrations of flue gas substances that could pass from flue gas into cultivation medium for algae cultivation to measure the changing composition of the flue gas in the closed experimental bioreactor system. The processed data from experimental measurements are presented and are discussed. The change in the composition of the culture medium for algae (without the presence of algae) due to flue gas aeration was monitored in the experimental part. It includes also the measurement of the change in the composition of the flue gas in time. All obtained and collected information are then summarized and discussed in the final part of the master`s thesis.
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Photosynthetically produced hydrogen
Osipenko, Elena ; Němcová, Yvonne (advisor) ; Ráček, Jan (referee)
Bio-hydrogen from microalgae and cyanobacteria has attracted commercial awareness due to its potential as an alternative, reliable and renewable energy source. Photosynthetic hydrogen production from algae and cyanobacteria can be interesting and promising options for clean energy, because hydrogen is produced at ambient temperature and pressure and releases only water as a by-product. The energy of sunlight is used to split the water molecule into protons (H+), electrons (e-) and O2. The protons and electrons are then recombined with the help of the enzymes hydrogenase or nitrogenase (in the case of cyanobacteria) to form H2. Both of these enzymes are sensitive to O2 and therefore require the processes of H2 formation and CO2 fixation to be separated. This bachelor's thesis aims to describe the principle of H2 formation in different cyanobacterial and algal systems and mention the problems and limitations. The thesis also presents recent approaches, including genetic and metabolic engineering of cyanobacteria and algae or co-culturing of algae and bacteria, where an anaerobic environment is created by enhanced bacterial respiration.
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Možnosti využití tuků při zpracování řas
Lučivňák, Roman
This work is a literature review and describes algae cultivation from available sources with a focus on closed cultivation systems. It is also dedicated to increasing the production of fats and their extraction from biomass. The entire thesis then focuses on the food use of these technologies and their products, and it also marginally describes the metabolism and basic taxonomy of algae.
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