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Effect of dysbiosis on proportion of particular neutrophil subsets and their functional capacities
Sklenářová, Lydie ; Hrdý, Jiří (advisor) ; Dobeš, Jan (referee)
The gut microbiota is crucial for maintaining physiological balance and influences metabolic processes, immune responses, and intestinal barrier function. Dysbiosis, or the imbalance of microbial composition, is associated with a range of health complications, including chronic inflammatory conditions such as non-specific intestinal inflammations. Inflammatory processes associated with dysbiosis and changes in microbial metabolites can directly affect the activation of neutrophils, impacting the pathogenesis of various diseases. Probiotics, defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host, offer the potential for positive modulation of these inflammatory conditions The aim of this thesis was to explore how experimentally induced intestinal dysbiosis affects the heterogeneity of neutrophils in the bone marrow. Dysbiosis was induced by administering antibiotics to mice, which were subsequently treated with the probiotic strain Escherichia coli O83:K24:H31 (EcO83). Neutrophil phenotypes were assessed using flow cytometry based on the expression of surface markers CD11b, Ly6G, CD62L, and CXCR2. Meanwhile, gene expression related to their antimicrobial functions and the inflammatory environment was analyzed by quantitative PCR. The results...
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Legal context and implications: analysis of blockchain regulation in selected jurisdictions
Bőhm, Erik ; Kučera, Zdeněk (advisor) ; Lederer, Vít (referee)
1 Legal context and implications: analysis of blockchain regulation in selected jurisdictions Abstract Blockchain technology has become a transformative force, offering transparency, security and efficiency across industries. However, its rapid growth brings regulatory challenges. This paper examines blockchain regulation, focusing on the European Union (EU) framework and comparative analysis with selected jurisdictions around the world. At the outset, the thesis seeks to clarify the basic concepts of blockchain and its regulatory implications. It tries to introduce the issues and frame the most important terms in a non- technical way. The thesis then examines EU legislation, including contemporary and forthcoming secondary regulations such as the Markets in Cryptoassets Regulation (MiCAR), and explores their objectives, challenges and implications for innovation and market participants. In addition, the paper conducts a comparative analysis of blockchain regulation in various jurisdictions, including the United States, the Cayman Islands and Singapore. By comparing regulatory regimes, it identifies trends, best practices, and ultimately a plethora of differences. It offers insight into regulatory preferences and implications for stakeholders, with an emphasis on business corporations and entrepreneurs in...
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Ability of plants to adapt to anthropogenic pollution
Vachek, Tadeáš ; Münzbergová, Zuzana (advisor) ; Dostál, Petr (referee)
Due to global climate change and human interventions, the environment is changing rapidly. This brings many stress factors that organisms must adapt to. One of the adaptive mechanisms currently widely discussed is rapid evolution, i.e., hereditary change based on natural selection occurring at an ecologically relevant pace. This work deals with the synthesis of knowledge about rapid evolution, the methodology of its research, and its role in the adaptive processes of plants to anthropogenic pollution, focusing on heavy metals in soil and artificial light at night. The collected studies indicate that heavy metals exert strong selective pressure that can lead to the separation of a tolerant population from the original within a few generations. However, the significance of artificial light at night as a selective factor for plant evolution has not yet been studied. Although individual studies deal with adaptive evolution in phototactic insects in response to artificial light at night, extensive research has not been conducted here either. From the general examination of the effects of artificial light at night on organisms (especially animals), both direct and indirect impacts on their functioning are evident, many of which have not yet been described in detail. Thus, the direct and indirect impacts...
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Laboratory diagnostics of hepatitis E - experience from routine laboratory
Tužáková, Andrea ; Janďourek, Ondřej (advisor) ; Diepoltová, Adéla (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Author: Andrea Tužáková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title thesis: Laboratory diagnosis of hepatitis E - personal experience in a routine laboratory This thesis takes a comprehensive review of viral hepatitis E (VHE), including theoretical and practical aspects. In the theoretical part, the VHE is presented in detail, including epidemiology, genotypic variants, clinical symptoms, diagnostic methods, prevention and treatment. The epidemiological trends and prevalence of VHE are analysed, along with genotypic variants and their correlation with clinical manifestations. Various diagnostic modalities and therapeutic options in the context of VHE are discussed. Hepatitis E is a liver infection caused by the hepatitis E virus. It is one of the five main types of hepatitis viruses: A, B, C, D, and E. Hepatitis E virus is transmitted mainly through the faecal-oral route, often via contaminated water or food. It can cause acute illness characterized by jaundice (yellowing of the skin and eyes), dark urine, fatigue, abdominal pain, and loss of appetite. Most cases of hepatitis E resolve on their own within a few weeks to months without causing...
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Results evaluation of reverse shoulder arthroplasty during physiotherapeutic intervention
Roztočilová, Dominika ; Fulín, Petr (advisor) ; Plecháčová, Marie (referee)
BACHELOR THESIS ABSTRACT Author: Dominika Roztočilová Supervisor: doc. MUDr. Petr Fulín, Ph.D., MBA Consultant: Ing. Milan Šebek Title: Results evaluation of reverse shoulder arthroplasty during physiotherapeutic intervention Abstract: This bachelor thesis deals with the topic of reverse shoulder arthroplasties and functional outcomes of the shoulder joint after the implantation. The aim of this thesis is to apply described physiotherapeutic approach to a selected group of patients and to evaluate the outcomes of shoulder joint function at given intervals during the postoperative physiotherapeutic intervention. The aim of the thesis is also to analyse current studies about this topic, compare the results and compare the effectiveness of different physiotherapeutic approaches. The theoretical part summarizes knowledge about the anatomy, kinesiology of the shoulder girdle and biomechanics of the shoulder joint. It also discusses shoulder replacements, especially the reverse implants. Furthremore, principles of physiotherapeutic postoperative intervention are described and physiotherapeutic approaches are outlined. The practical part of the thesis is procesed in the form of a research. It includes 8 patients who underwent implantation of a reverse arthroplasty and subsequent physiotherapeutic intervention. For...
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Variability of Lepidium campestre seed mass concerning nutrition
Tučková, Kristýna ; Mašková, Tereza (advisor) ; Dostál, Petr (referee)
Seed production allows plants to maintain their genetic diversity, survive in dormancy through unfavourable conditions and disperse to new distant sites. Seed's own size reflects the amount of provisions designated for its germination, thus the rate and success of its germination. Seed size can also suggest dispersion distance, a chance to avoid seed predators, and how likely the seedling will withstand unfavourable conditions right after germination and before the formation of necessary structures for growth independent of maternal supplies. In general, the more nutrients a plant acquires during seed production, the bigger the number or size of seeds it can produce. In some cases, more nutrient uptake can lead to the production of more numerous and bigger seeds simultaneously, however, the direction of increased investment in reproduction highly depends on plant species. This work studies the effect of nutrient gradient in the environment on the variability of seed size in Lepidium campestre. It explores how seed traits change along the maternal environmental nutrient gradient, specifically how their number, size and seed coat thickness responds to the gradient. Subsequently, this work discusses the persistence of the maternal nutrient effect in the second generation, phenomenon called the...
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Development of LC-MS method for compounds used for determination of cytochrome activity
Smolík, Martin ; Lochman, Lukáš (advisor) ; Horáček, Ondřej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Bc. Martin Smolík Supervisor: PharmDr. Lukáš Lochman, Ph.D. Consultant: prof. PharmDr. Petr Pávek, Ph.D. Title of thesis: Development of LC-MS method for compounds used for determination of cytochrome activity. Cytochromes P450 (CYP450) are enzymes that play a key role in the metabolism of xenobiotics and some endobiotics (cholesterol, steroid hormones, and eicosanoids). They are present in the liver, gastrointestinal tract, lungs, and kidneys. Determination of CYP450 activity is a valuable tool to detect drug interactions resulting from inhibition or induction of various isoforms of this enzyme. This thesis is devoted to the development and validation of a new analytical method using liquid chromatography coupled with tandem mass spectrometry for the determination of activity CYP450 isoforms 3A4, 2C9, and 2B6, which are crucial for the metabolism of many drugs and biologically active substances. In the framework of this thesis, a new method was developed providing a fast, accurate, and reliable measurement of the activity of these enzymes based on the quantification of specific CYP450 substrates, namely midazolam (3A4), diclofenac (2C9), bupropion (2B6) and their...
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The testing of familiar relationship in the identification genetics
Bobková, Alena ; Vaněk, Daniel (advisor) ; Zemanová, Zuzana (referee)
This bachelor's thesis analyses kinship testing in identification genetics, which is a discipline enabling the genetic identification of individuals and the determination of genetic relatedness between them. This thesis covers a wide range of applications from forensic analysis through genealogical studies to biodiversity conservation. This field has been rapidly growing and gaining on importance since the 1980s, when the foundations of DNA fingerprinting were laid based on the discovery of hypervariable minisatellite regions in the genome. This thesis aims to provide an overview of methods and techniques that are used for genetic identification and kinship testing. The thesis discusses genetic markers such as STRs or SNPs, which are the basis for genetic profiling, are discussed. In addition statistical methods for evaluating genetic match and methodologies for determining the probability of kinship are also discussed. Finally, practical applications of identification genetics are presented, including its use in forensic science or in case of solving genealogical questions. The potential and future development of the technologies and their impact on society is discussed at the end of the thesis. Thus, the thesis contributes to a better understanding of the importance and opportunities of...
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Food composition and feeding selectivity of zooplankton
Lis, Jonáš ; Škaloud, Pavel (advisor) ; Čablová, Radka (referee)
Zooplankton are an important link in the food web between phytoplankton and higher tro- phic levels, and understanding their feeding preferences is the key to unraveling the functions and dynamics of aquatic ecosystems. Current knowledge of zooplankton food composition is limited, consisting mainly of laboratory experiments that have revealed different hunting strategies and selection of prey based on size and nutritional value. Recently, publications have emerged that, through the use of DNA metabarcoding, provide a completely new and comprehensive insight into the diversity of zooplankton diet composition, especially copepods, cladocerans and rotifers. These methods reveal that the feeding niches of copepods and cladocerans are distinct and do not overlap. In general, both groups are said to prefer mainly diatoms as their food, when available. The food niches of rotifers are so diverse that no clear trend can be established for the entire phylum. Cyanobacteria appear to be the main source of energy for zooplankton and are proba- bly consumed by metazooplankton largely as secondary prey when consuming ciliates and other heterotrophic prey. Furthermore, differences in feeding niches between species are known and can be partly explained by their vertical distribution, and different levels of feeding...
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Nuns as caretakers? A look into nuns's activities in selected hospitals of the Czechoslovak Republic between 1948 and 1960
Štrofová, Kateřina ; Stehlík, Michal (advisor) ; Michela, Miroslav (referee)
(in english) The subject of this thesis are three female monastic communities and their activities in selected hospitals of socialist Czechoslovakia between the years 1948-1960. The aim is to examine the overall policy of the communist authorities towards sisters in the specific environment of hospitals and the stance of local authorities, i.e. the everyday practise in healthcare. Emphasis is placed on the nuns themselves, their professional relationships with doctors and civil nurses and their actions on the local level. The thesis also analyses church practise in hospitals, interventions of the state-appointed church secretaries and partly even their oversight of activity of the Catholic church in individual districts. Last but not least, large effort was expanded to reconstruct the sisters' own perception of post-1948 persecutions and interventions in medical care, not just in relation to the strategies, which they have chosen to cope with the adverse conditions within their communities, but also their mostly disapproving attitudes towards the interventions of the communist regime against their presence in hospitals.
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