National Repository of Grey Literature 30 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Development of LC-MS method for compounds used for determination of cytochrome activity
Smolík, Martin ; Lochman, Lukáš (advisor) ; Horáček, Ondřej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Bc. Martin Smolík Supervisor: PharmDr. Lukáš Lochman, Ph.D. Consultant: prof. PharmDr. Petr Pávek, Ph.D. Title of thesis: Development of LC-MS method for compounds used for determination of cytochrome activity. Cytochromes P450 (CYP450) are enzymes that play a key role in the metabolism of xenobiotics and some endobiotics (cholesterol, steroid hormones, and eicosanoids). They are present in the liver, gastrointestinal tract, lungs, and kidneys. Determination of CYP450 activity is a valuable tool to detect drug interactions resulting from inhibition or induction of various isoforms of this enzyme. This thesis is devoted to the development and validation of a new analytical method using liquid chromatography coupled with tandem mass spectrometry for the determination of activity CYP450 isoforms 3A4, 2C9, and 2B6, which are crucial for the metabolism of many drugs and biologically active substances. In the framework of this thesis, a new method was developed providing a fast, accurate, and reliable measurement of the activity of these enzymes based on the quantification of specific CYP450 substrates, namely midazolam (3A4), diclofenac (2C9), bupropion (2B6) and their...
The uset of HPLC in the field of chiral separations VIII.
Šlapáková, Tereza ; Horáček, Ondřej (advisor) ; Lochman, Lukáš (referee)
Charles University Faculty of Pharmacy in Hradci Králové Department: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Student: Bc. Tereza Šlapáková Supervisor: RNDr. Ondřej Horáček, Ph.D. Consultant: doc. PharmDr. Radim Kučera, Ph.D. Title of thesis: The uset of HPLC in the field of chiral separations VIII. Carboranes are highly stable, hydrophobic, inorganic compounds that are based on an icosahedral structure composed of boron atoms. Some carborane representatives are chiral, which is mainly due to asymmetric substitution. The establishment of reliable and rapid methods to enable the separation of the enantiomers is necessary, which is important for their future use in practice. The high-performance liquid chromatography method is advantageous for practical application as it is widely used, robust, and applicable to a large range of fields. The aim of this work was to test the behavior of chiral carboranes on polysaccharide columns in HPLC. First, screening was performed by gradient method using six chiral polysaccharide-based stationary phases, the component A of the mobile phase was 50 mmol/l NaClO4, and the component B was ACN or MeOH. Subsequently, the method was converted to isocratic elution. The purpose of this study was to optimize the conditions to create a database of...
Chiral separations of borane cluster compounds.
Horáček, Ondřej ; Kučera, Radim (advisor) ; Šatínský, Dalibor (referee) ; Bednář, Petr (referee)
Univerzita Karlova, Farmaceutická fakulta v Hradci Králové Katedra farmaceutické chemie a farmaceutické analýzy Kandidát: Mgr. Ondřej Horáček Školitel: doc. PharmDr. Radim Kučera, Ph.D. Název: Chirální separace klastrových sloučenin boru Anorganické trojrozměrné klastrové sloučeniny boru se staly předmětem zájmu vědců zejména díky svým zajímavým vlastnostem, např. výjimečné tepelné, biologické a chemické stabilitě, neobvyklé třístředodové dvouelektronové vazbě a trojrozměrné aromaticitě. Podtřídou boranových klastrů jsou karborany, které mají v kleci inkorporován alespoň jeden atom uhlíku, čímž kombinují vlastnosti anorganických a organických látek v jedné molekule. Atom uhlíku umožňuje modifikaci klastru různými organickými substituenty, což vede k existenci nepřeberného množství derivátů. Z tisíců těchto sloučenin byly důkladně zkoumány deriváty aniontového bis(dikarbolidu) kobaltu ([Co(C2B9H11)2]- ), a to zejména ve farmaceutické chemii, chemii materiálů, elektrochemii a analytické chemii. Zvýšený zájem o [Co(C2B9H11)2]- je obvykle vysvětlován jeho rozpustností ve vodě, chemickou a biologickou stabilitou, nízkou toxicitou, skrytým amfifilním charakterem a podobností s chemií ferrocenu. Mnoho derivátů [Co(C2B9H11)2]- uvedených v literatuře je chirálních. Navzdory jejich potenciálnímu využití ve...
Chiral separations of borane cluster compounds.
Horáček, Ondřej ; Kučera, Radim (advisor) ; Šatínský, Dalibor (referee) ; Bednář, Petr (referee)
Univerzita Karlova, Farmaceutická fakulta v Hradci Králové Katedra farmaceutické chemie a farmaceutické analýzy Kandidát: Mgr. Ondřej Horáček Školitel: doc. PharmDr. Radim Kučera, Ph.D. Název: Chirální separace klastrových sloučenin boru Anorganické trojrozměrné klastrové sloučeniny boru se staly předmětem zájmu vědců zejména díky svým zajímavým vlastnostem, např. výjimečné tepelné, biologické a chemické stabilitě, neobvyklé třístředodové dvouelektronové vazbě a trojrozměrné aromaticitě. Podtřídou boranových klastrů jsou karborany, které mají v kleci inkorporován alespoň jeden atom uhlíku, čímž kombinují vlastnosti anorganických a organických látek v jedné molekule. Atom uhlíku umožňuje modifikaci klastru různými organickými substituenty, což vede k existenci nepřeberného množství derivátů. Z tisíců těchto sloučenin byly důkladně zkoumány deriváty aniontového bis(dikarbolidu) kobaltu ([Co(C2B9H11)2]- ), a to zejména ve farmaceutické chemii, chemii materiálů, elektrochemii a analytické chemii. Zvýšený zájem o [Co(C2B9H11)2]- je obvykle vysvětlován jeho rozpustností ve vodě, chemickou a biologickou stabilitou, nízkou toxicitou, skrytým amfifilním charakterem a podobností s chemií ferrocenu. Mnoho derivátů [Co(C2B9H11)2]- uvedených v literatuře je chirálních. Navzdory jejich potenciálnímu využití ve...
Physiotherapy procedures in patients after disc surgery in lumbar spine
Kozohorská, Václava ; Babková, Lenka (advisor) ; Horáček, Ondřej (referee)
This thesis focuses on problems of physiotherapy procedures after a surgery of intervertebral disc in the meaning of intensity, timing and therapeutic procedures. In the general part it gives comprehensive overview about functional anatomy, kinesiologi, biomechanics of the spine and about the function of intervertebral disk. It also deals with methods of investigation and surgical interventions. In the special part, the thesis focuses on the most appropriate choice of timing, intesity and mainly on special methods which are the most commonly used in patients after surgery of intervertebral disc. It gives the basic charakteristics of each special methods in relation to patients after surgery of intervertebral disc. The practical part of this thesis includes patient case report after surgery of intervertebral disc which describes the examination before neurosurgical intervention, surgery, hospitalization and outpatient rehabilitation.
Deviation of the axis of the spine in hereditary motoric and sensitive neuropathy
Horáček, Ondřej ; Kučera, Miroslav (advisor) ; Druga, Rastislav (referee) ; Bojar, Martin (referee) ; Kittnar, Otomar (referee)
Deviation of the axis of the spine in hereditary motoric and sensitive neuropathy Powered by TCPDF (www.tcpdf.org)
Utilization of the balance therapy in patients with CMT disease
Palacký, Vít ; Gocalová, Lucie (advisor) ; Horáček, Ondřej (referee)
Utilization of the balance therapy in patients with CMT disease Powered by TCPDF (www.tcpdf.org)
Calculations of parameters of biologically active compounds by the quantum mechanical methods.
Horáček, Ondřej ; Palát, Karel (advisor) ; Krátký, Martin (referee)
8 ABSTRACT The aim of this work is to calculate the parameters of substituted bases and chosen phenylguanidinium cations, earlier synthesized at the Department of inorganic and organic chemistry on the Faculty of Pharmacy CU in Hradec Králové, using quantum chemical methods. I solved chosen problem by using computer programs Gaussian 03W and Hyperchem 8.0.10. I used program Gaussian to optimize the molecules and calculate the energy of the HOMO and LUMO, total energy and the atomic partial charge using the Mulliken population analysis. I used programe HyperChem to calculate the Van der Waals molecular volume; Van der Waals molecular surface; logP; the molar refraction; solvent-accessible surface of the probe size 1.0 Å; 1.2 Å; 1.4 Å; 1.6 Å and the atomic partial charge using methods PEOE. All parameters of selected substituted phenylguanidines above have been successfully calculated, and listed in the tables in the chapter called results. The calculated results were discussed in chapter called discussion. The calculated parameters will be used for further correlations and QSAR studies that will help to understand the biological activity of substituted phenylguanidines and hence their effect on the human organism.
Examination of vegetative system in hereditary neuropathy
Jílková, Daniela ; Horáček, Ondřej (advisor) ; Kobesová, Alena (referee)
Aim: The aim of this thesis "Examination of vegetative system in hereditary neuropathy" is to consider the level of physical fitness, physical actvity level and quality of autonomic function in patients with hereditary neuropathy Charcot Marie Tooth and an assessment of interactions of these parameters, especially with regard to possible influence by the presence of autonomic neuropathy and with regard to neurological symptoms. Background: Although vegetative neuropathy was mentioned in the first publication about CMT, it is not widely discussed topic as part of it. Methods: Seventeen probands underwent spiroergometry, heart rate variability test, six minute walk test, rating by the CMT Neuropathy Score and Overall neuropathy disability scale and completing the IPAQ questionnaire. Results: We found symptoms of autonomic neuropathy in group of patients with CMT, especially high-frequency part of heart rate variability spectrum has a particularly significant decrease. Patients with autonomic neuropathy have shown a low fitness and functional capacity, tendency to obesity and hypertension. The observed correlation of autonomic neuropathy and neurological impairment was very weak. Conclusions: We find a neuropathy of the autonomic nervous system in patiens with hereditary motor and sensitive neuropathy...
Gait disorders in hereditary neuropathy
Linhartová, Petra ; Horáček, Ondřej (advisor) ; Truc, Michal (referee)
Hereditary motor sensory neuropathy (CMT) exhibits characteristic symptoms, i.e. specific gait impairment. Little detailed information has been written on the gait analysis of this disease. The aim of this work was to collect information about gait disorders in patients with CMT, to perform a threedimensional gait analysis and prove the effectiveness of rehabilitation. There was a detailed kinesiological analysis, muscle strength test and Coda Motion System gait analysis done on one patient. The patient has visited rehabilitation for 12 weeks and after, the whole spectrum of examinations were repeated. Within the framework of the gate analysis there were the joint ranges in flexion-extension of the hip, knee and ankle evaluated and compared with the normal gait. After undergoing rehabilitation there was subjective improvement in the quality of walking and objective improvement compared to the initial examination's findings. This acknowledges the results of the kinesiological analysis, muscle strength test and Coda Motion System gait analysis. Results of this work provide an overview of the patterns of gait disorders in CMT and support the importance and effectiveness of rehabilitation in improving gait quality in CMT patients. Powered by TCPDF (www.tcpdf.org)

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