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Preparation of the 14-3-3 Protein Binding Partners for Structural Studies.
Kopecká, Miroslava ; Obšil, Tomáš (advisor) ; Teisinger, Jan (referee)
Tyrosine hydroxylase belongs to the group of hydroxylases of aromatic acids and catalyzes a key step in the biosynthesis of catecholamine neurotransmitters. The tyrosine hydroxylase possesses the homotetrameric structure and contains three structural domains: the N-terminal regulatory domain, the catalytic domain and the C-terminal tetramerization domain. The activity of tyrosine hydroxylase is regulated by phosphorylation and through the regulation of its expression. Phosphorylation at Ser-19 induces binding of the 14-3-3 protein, which affects the structure of the regulatory domain and protects it against both dephosphorylation and degradation. Since the structure of the regulatory domain is still unknown, we decided to perform its structural characterization using NMR techniques. First, the expression and purification protocol of the regulatory domain of tyrosine hydroxylase was optimized. The protein was expressed as a His-tag fusion protein and its purification is composed from two steps: the chelating chromatography and the size-exclusion chromatography. The dynamic light scattering and the 1 H nuclear magnetic resonance were used to verify its monodispersity, and hence its suitability for further experiments.
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Prolyl endopeptidase of the blood fluke Schistosoma mansoni
Fajtová, Pavla ; Konvalinka, Jan (advisor) ; Ryšlavá, Helena (referee)
Prolyl endopeptidase SmPEP from the blood fluke Schistosoma mansoni is investigated here for the first time. This enzyme is potentially interesting as a drug target for the treatment of schistosomiasis. SmPEP was detected in the extract of adult worms by enzyme activity and immunoreactivity. Enzymatically active SmPEP was produced in the E. coli expression system and was chromatographically purified. The pH optimum of recombinant SmPEP was about 8. Substrate specificity analysis revealed that SmPEP cleaved peptide substrates by endopeptidase activity, however, macromolecular substrates were not fragmented. The residue preferences in the positions P3 to P1' were determined using synthetic fluorogenic peptide substrates. SmPEP was found to be highly sensitive to the inhibition by Z-Ala-Pro-CMK and Z-Arg-Pro-CHO. Primary screening of crystallization conditions for recombinant SmPEP was performed. " (In Czech)"
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Representative of drama education in South Bohemia - Jirina Lhotská
NOVOTNÝ, Ivan
The thesis deals with the work of Mgr. Jiřina Lhotská and her contribution to the drama in education in the South Bohemia County. The process of drama in education teaching and its basic elements are described in the theoretical part of the thesis. In the second part focuses on the character of Jiřina Lhotská, her career and contribution to the drama in education teaching. Furthermore, the practical part also contains interviews with individuals who profoundly influenced Jiřina Lhotská the pedagogue. The interviews with her colleagues and students with whom she has cooperated during various stages of her career (up until now) is also included therein. The lists of Jiřina Lhotská's creative activities, selected scripts of certain acts, as well as some photographs form part of multiple appendices.
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Psychotherapeutic aspects of author's creation
MIKULOVÁ, Renáta
This Bachelor thesis dealing with the author's creation was written as a potential psychotherapeutic method. The goal of the thesis is to determine whether writing own short texts and reading them in public can be used in the area of psychotherapy or not. Further goal is a description of the relationships between the individual and the author's creation. The author has been also analysing external and internal effects influencing the individual. The thesis is divided into two parts a theoretical and a practical part. In the theoretical part the author describes the author's creation itself, the game phenomenon and the area of psychotherapy. There is a thorough description of narrative and expressive psychotherapy particularly dramatherapy, poetry therapy, bibliotherapy, fairytale therapy and game therapy. Furthermore, there is mentioned existing knowledge of author's creation including the research done at University of South Bohemia in České Budějovice. The practical part is basically a qualitative study which is further divided into two parts. The first part is concerned with analysis of semistructured interviews, the second part with analysis of student's self-reflections. Summary and comparison of the research results and author's own case study are at the end of the thesis. Subject of discussion is an application of the author's creation in the area of expressive psychotherapy.
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Self-concept of an individual in dance movement
ZOUZALOVÁ, Kristýna
This diploma thesis deals with self-concept of a maturing individual in dancing. We look into the way of self-perception of a dancer during their commitment to dancing as a leisure time activity in their process of growing up. In the theoretical part, the thesis deals with defining the terms related to movement, dancing and its various psychological and pedagogical aspects, especially expression and expressivity. The theoretical part also focuses on particular components of self-concept. The practical part contains case studies based on qualitative research, which stem from data achieved by in-depth interviews and introspection and retrospection done by the author of the thesis.
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DNA čipy a jejich využití pro molekulární diagnostiku a predikci u onkologických onemocnění
VORÁČKOVÁ, Lucie
Many visible factors produced during carcinogenesis refer to oncological disease, e.g. modified gene's expression or increased number of miRNA. These factors can be diagnosed by analyzing of gene's expression. For this diagnostics, there are used many methods, of whom DNA microarray analysis is the most significant progress in genetic research. A large number of DNA sequences can be detected DNA with microarrays on a single microarray in a relatively short time. This analysis means a positive turnover for the diagnosis and prediction of disease.
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Nucleotide density for finding CpG islands
Sikorová, Eva ; Provazník, Ivo (referee) ; Maděránková, Denisa (advisor)
This bachelor thesis deals with searching for CpG islands in the DNA sequences using the nucleotide density vectors. The first part includes view of the DNA structure, the genetic information expression, more detailed analysis of CpG islands and primarily their detection methods. In the practical part the algorithm for graphical representation of nucleotides on the basis of their densities and detections of CpG islands of artificially created and real DNA sequences was realized in the MATLAB program. The thesis includes the analysis of twenty sequences with the expected content of CpGIs and the comparison of results between the created program and two search engines.
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Transformation of Equation Editor Expressions to LaTeX
Šimek, Jiří ; Jirák, Ota (referee) ; Křivka, Zbyněk (advisor)
The bachelor thesis deals with the problem of transformation of Equation editor expressions to LaTeX. It presents description of Equation editor expressions in Word 2007, it subsequently focuses on the syntax of particular expressions and it suggests the ways of their transformation into LaTeX. The grammar, which was used for the implementation of the converter, is designed on the basis of the syntax of expressions.
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Translation of C Expressions to DIMACS Format
Grim, Pavel ; Charvát, Lukáš (referee) ; Smrčka, Aleš (advisor)
This work focuses on proposition of transfer of the expressions entered in the C programming language into DIMACS format and creation of program in programming language C++ making this transfer. This work contains a description of the C programming language and its operators. It also contains a description of the conjunctive normal form and a description of the DIMACS format. Following is a proposal for a program for the transfer of expression in the C programming language to the DIMACS format and description of realization of program performing this transfer.
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Spectres and Illusions
Arendárik, Ján ; Stratil, Václav (referee) ; Artamonov, Vasil (advisor)
The subject of my practical diploma thesis is a painting reaction to the photographs from years 1915 to 1930. I see them as a " photographs of dead(lost) world. The photographs of people whose dont live in present but the photographs are evidence of their existence in the past.
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