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Mammalian proteins carrying zinc finger arrays
Vobruba, Adam ; Svoboda, Petr (advisor) ; Mašek, Jan (referee)
A zinc finger is a small peptide motif stabilised by a single zinc ion, best known for its capability to specifically bind a 3-nucleotide sequence of DNA, depending on the exact amino acids present in the DNA-binding positions. Zinc fingers are unique in their ability to freely link together and form longer tandem arrays, which can bind DNA targets of any length and sequence determined by the combination of individual fingers. These arrays can easily mutate and be rebuilt to change binding specificity, allowing great flexibility and helping zinc fingers to their widespread presence in numerous endogenous proteins of various functions. This property of zinc finger arrays also made them a suitable tool for the creation of custom DNA- binding domains for genetic engineering. This thesis provides an overview of the discovery, structure and function of these domains and then reviews and discusses selected naturally occurring mammalian zinc finger proteins and their properties, showcasing diverse uses zinc finger arrays have been adapted for throughout evolution. The history and future of zinc fingers in artificial proteins created for gene therapy and research are discussed as well. Keywords: zinc finger, ZnF, KRAB, KZFP, CTCF, PRDM9, ZFN, mammals
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Spatio-temporal activity of African ungulates at water sources in Mogalakwena River Reserve, South Africa
Homoláč, David ; Hořák, David (advisor) ; Bennitt, Emily (referee)
The large mammals of African savannah are a well-studied group of animals, but few studies focus on the temporal, circadian activity of this group of mammals. Such studies either use problematic methods of data collection (webcams, direct observations) or have been conducted in national parks or large reserves, where movement of the animals is not limited with anthropogenic structures. In such sites, the community of large mammals is usually complete, including the guild of large carnivores. Thus, small, fenced off reserves without a complete community of large mammals are understudied. Therefore, this work focuses on the spatio-temporal activity of ungulates at water sources in the Mogalakwena River Reserve in South Africa, which is an example of a reserve without large predators except the leopard (Panthera pardus). I used camera traps to assess the activity of ungulates, which I placed alongside the Mogalakwena River and at the water holes. With the help of artificial intelligence, I classified the photos to the level of activity of each species. I then tested this activity over space (type of water source) and against biotic and abiotic factors. The activity of ungulates at water sources is influenced by the maximum daily temperature, as the activity shifts to the evening hours during higher...
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Role of melatonin in the mammalian intestine and its interaction with the gut microbiome
Přiklopilová, Barbora ; Sumová, Alena (advisor) ; Mašín, Jiří (referee)
Melatonin is a hormone that primarily functions in regulating circadian rhythms in mammals. Independently of the pineal gland, it is also synthesized in the intestines, where it can interact with the gut microbiome and thus contribute to a wide variety of diverse functions. The aim of this paper is to describe the role of melatonin and summarize its functions across different organisms, with a particular focus on mammals. The work concentrates on explaining the proven effects of melatonin in the mammalian gut, its potential interactions with the gut microbiome, and the overall impact on the homeostasis of the intestinal environment.
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Circumstances of finding and comparison of health care for different bird and mammal taxa at a rescue station
HUBÁČKOVÁ, Daniela
This thesis is focused on the Wildlife Rescue Station Makov (county Písek). The thesis presents an overview to the rescue station activities during the observation period 2016-2022. The practical part is dedicated to the analyses concerning the birds and mammals admitted to the rescue station. The analyses stand out from the individual reasons for admission, the success of treatment, the time of care for each order and the impact of whether the animals thrive more when they are singly or in a group. The thesis also introduces the National Rescue Network, its activities, history and funding, and the environmental education of the stations. The thesis is enriched by some of the reasons for intake, which include injuries caused e.g. by power lines, traffic or in any way disadvantaged animal (e.g. injury).
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Cryptids
Dvořáková, Dana ; Turek, Filip (referee) ; Sterec, Pavel (advisor)
Cryptids, video which is studying animals, its shapes, moves and the environment , where they find themselves. The video is looking equal to all entity without any difference, document varied life as same as passing death or rot as well. It also speaks about collaboration and the microworld, which creates perfect unit. Silent mutual conversations.
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Liver cells regeneration in mammals
Ťažký, Timotej ; Tlapáková, Tereza (advisor) ; Onhajzer, Jakub (referee)
Liver cell regeneration is an important biological process that allows mammals to maintain liver function while recovering from liver damage. Liver cell proliferation serves as the primary mode of liver regeneration, which in hepatocytes is activated by the transition from the G0 to G1 phase of the cell cycle. Proliferation is also promoted by non-parenchymal liver cells among which include Ito cells, Kupffer cells, and endothelial cells of hepatic sinusoids. In a comprehensive analysis of key signaling pathways, it was clearly demonstrated that the Wnt/β catenin, Notch, Hippo, NF-κB, and Hedgehog signaling pathways play a key role in the regulation of liver cell proliferation and differentiation during regeneration. The regenerative potential of the liver is influenced by various factors such as age, extent of damage and health conditions. Additionally, the remarkable regenerative capacity of the liver has clinical implications in the context of liver transplantation, partial hepatectomy and the treatment of liver diseases such as cirrhosis, hepatitis and hepatocellular or cholangiocellular carcinoma. Modulation of key signaling pathways and identification of novel molecular targets can improve the clinical outcomes of patients with liver diseases or even accelerate the entire process of liver...
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Evolution of litter size in hystricomorph rodents
Cingelová, Alexandra ; Dušek, Adam (advisor) ; Špoutil, František (referee)
The suborder Hystricomorpha is an ideal model group for the study of rodent life-history evolution due to its wide distribution, great diversity of inhabited ecosystems and social systems. By tracking several factors, it was possible to determine the key factors that influenced litter size during evolution. The data set contained 75 of the total 292 species of the suborder. The average litter size, female and male body size, level of sexual dimorphism, level of sociality, type of reproductive system, level of parental care, presence of multipaternity, type of habitat and food were assigned to each species based on the information obtained from the available literature. The analysis included a statistical part calculated using general linear mixed models, and a phylogenetic part including parsimonious mapping of characters onto the phylogenetic tree and linear regression using phylogenetic independent contrasts. Social factors like degree of sociality, sexual dimorphism and reproductive system significantly influenced litter size at birth. Multipaternity also had an influence, but less than the first three factors. Ecological factors, food and environment, had no effects unless they were combined with the effect of family. It is therefore possible to assume that ecological factors have influenced...
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Typology and teeth determination of selected mammalian species
Černá, Adéla ; Říhová, Pavla (advisor) ; Pyszko, Martin (referee)
In this bachelor's thesis I am dealing with mammalian teeth. I have focused mainly on the teeth of certain species that are endangered and for which significant levels of illegal trade have been recorded. In selecting the species I focus on, I base my thesis on the analysis of data from the EU-TWIX and CITES Trade Database, which is compiled at the Environmental Forensic Sciences at the Faculty of Science, Charles University. The most endangered species traded include felines, canids and ursids. In this thesis, I mention how teeth can be identified, what an ideal tooth should look like, I describe what a tooth is made of, why teeth are traded at all, what they are used for, and how individual teeth can be confused with other mammal species. I also mention the Convention on International Trade in Endangered Species CITES. For this research I have largely used articles from abroad, available on the Web of science. Key words: teeth, mammals, determination
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Molecular basis of the sperm selection in the mammalian female reproductive tract
Šrámková, Adéla ; Krylov, Vladimír (advisor) ; Postlerová, Pavla (referee)
Internal fertilization is typical for all mammals. The male transfers sperm cells into the female reproductive tract, where usually one of them reach the oocyte and fertilize it. The female reproductive tract must be adapted to provide a suitable environment for the sperm cells to survive until fertilization. On the other hand, it must also be able to select developmentally competent sperm cells and prevent others to get into the close contact with the oocyte. Physical and molecular mutual communication of sperm cells with the female reproductive tract is ensured by sperm surface molecules and the receptors of the female epithelia lining. It appears that these interactions are required for key transformations, both on the sperm surface and in the cells of the female reproductive tract, where gene expression is affected. As a result of these changes, the most suitable sperm cell with all the required characteristics is allowed to fertilize the matured oocyte.
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