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	Různé strategie pro rekombinantní produkci Leitner, C. ; Ertl, S. ; Volc, Jindřich ; Haltrich, D. ; Ludwig, R. The fungal enzyme pyranose 2-oxidase (EC 1.1.3.10) is the key catalyst for production of 2-ketoglucose, a glucose derivative of industrial interest. To prevent formation of inclusion bodies during its heterologous expression in E. coli, several fermentation protocols were developed to ensure proper folding and recovery of the enzyme activity at high levels Detailed record
	Purifikace a charakterizace penicilin-G-acylasy exprimované v Escherichia coli a Pichia pastoris Marková, Zdenka ; Marešová, Helena ; Kyslík, Pavel The pga gene coding for penicillin G acylase from strain E. coli RE3 was cloned on pPICZA plasmid and expressed in the yeast Pichia pastoris X33. The enzyme was purified from both strains and further characterized Detailed record
	Správné a chybné skládání chimérní pyranosa-2-oxidasy exprimované v E. coli Marešová, Helena ; Kyslík, Pavel Recombinant and chimeric pyranose 2-oxidases from different parental strains (Trametes ochracea and pubescens) were expressed in E. coli and formation of inclusion bodies were followed. The two, closely-related enzymes differ in folding into an active form under identical growth conditions Detailed record
	Různé strategie pro rekombinantní produkci Leitner, C. ; Ertl, S. ; Volc, Jindřich ; Haltrich, D. ; Ludwig, R. The fungal enzyme pyranose 2-oxidase (EC 1.1.3.10) is the key catalyst for production of 2-ketoglucose, a glucose derivative of industrial interest. To prevent formation of inclusion bodies during its heterologous expression in E. coli, several fermentation protocols were developed to ensure proper folding and recovery of the enzyme activity at high levels Detailed record
	Vliv limitace isoleucinem na hladinu prekursorů valinu a na aktivitu synthasy acetohydroxykyselin u kmenů Corynebacterium glutamicum rel+ a rel- Denina, I. ; Paegle, L. ; Ruklisha, M. ; Prouza, Marek ; Pátek, Miroslav Isoleucine limitation was found to increase acetohydroxyacid synthase activity in Corynebacterium glutamicum cells. Similar increase of activity of this enzyme was observed in C. glutamicum rel+ strains as well in rel- mutant Detailed record
	Aktivace translačního systému v průběhu buněčné diferenciace streptomycet Bobek, Jan ; Palečková, Petra ; Vohradský, Jiří ; Mikulík, Karel Synchronously germinating spores of S. granaticolor were used to study the activation of translational system and expression of proteins. Proteomic approach was used to gain more information on expression of proteins during germination on two types of media differing in ability of cells to produce antibiotic granaticin. The requirement of functional trans-translational system for reactivation of translation in S. aureofaciens exposed to unfavourable conditions was examined. Abundance of tmRNA is connected with changes in the rate of translation and with the accumulation of tetracycline. Our data indicate that trans-translation could be involved in self-defence against antibiotics synthesized Detailed record
	Stanovení stopového množství linkomycinu ve fermentační tekutině modifikovaných kmenů Streptomyces metodou HPLC a UPLC Olšovská, Jana ; Jelínková, Markéta ; Man, Petr ; Flieger, Miroslav ; Koběrská, Markéta UPLC and HPLC method development for lincomycin determination in fermentation broth (sample matrix) in concentration range 0.1-100 µg/ml. Method application for lincomycin determination after different types of genetic manipulations in lincomycin biosynthetic cluster Detailed record
	Sekundární metabolity druhů Geosmithia sdružených s hmyzem: polyhydroxylované antrachinony G. lavendula a perspektivy další práce Kolařík, Miroslav ; Stodůlková, Eva ; Šulc, Miroslav ; Kuzma, Marek ; Man, Petr ; Flieger, Miroslav ; Křesinová, Zdena Cultures of G. lavendula produce various colored hydroxylated antraquinones (AQ). Rhodolamprometrin and pentahydroxy-AQ were identified so far. Natural AQs are used as dyes and pharmaceuticals Detailed record
	Skryté klustery genů sekundárního metabolizmu u Streptomyces ambofaciens Nezbedová, Šárka ; Karray, F. ; Juguet, M. ; Aigle, B. ; Pernodet, J. L. ; Weiser, Jaroslav We looked for conditions leading to activation of silent gene clusters implied from the sequence as responsible for biosynthesis of yet unknown secondary metabolites in mutants blocked in biosynthesis of known antibiotics (spiramycin, congocidin). This approach should contribute to the better understanding of interaction between primary and secondary metabolism during production of antibiotics and the role of morphological differentiation in these processes Detailed record
	Nový kultivační systém složený ze skleněných kuliček ponořených v kapalném médiu využívaný pro proteinové studie diferenciace Streptomycet Weiser, Jaroslav ; Nguyen, Liem Duy ; Holub, Martin ; Nezbedová, Šárka ; Virolle, M. - J. ; Kalachová, Ladislava ; Benada, Oldřich ; Kofroňová, Olga ; Bezoušková, Silvia To get a better understanding of the interplay between primary and secondary metabolism and morphological differentiation in Streptomycetes we studied their proteomes in cultivation system resembling their natural environment Detailed record

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