Original title: Different strategies for the recombinant production
Translated title: Různé strategie pro rekombinantní produkci
Authors: Leitner, C. ; Ertl, S. ; Volc, Jindřich ; Haltrich, D. ; Ludwig, R.
Document type: Papers
Conference/Event: International Symposium on the Genetics of Industrial Microorganisms /10./, Praha (CZ), 2006-06-24 / 2006-06-28
Year: 2006
Language: eng
Abstract: [eng] [cze]
The fungal enzyme pyranose 2-oxidase (EC 1.1.3.10) is the key catalyst for production of 2-ketoglucose, a glucose derivative of industrial interest. To prevent formation of inclusion bodies during its heterologous expression in E. coli, several fermentation protocols were developed to ensure proper folding and recovery of the enzyme activity at high levels Enzym pyranosa-2-oxidasa z hub (EC 1.1.3.10) je klíčovým katalyzátorem pro produkci 2-keto-glukosy, průmyslově zajímavého derivátu glukosy. Aby bylo možno eliminovat tvorbu inklusních tělísek při jeho heterologní expresi v E. coli, byly vypracovány fermentační protokoly, které umožnily správné skládání proteinu a vedly k získání vysokých enzymových aktivit
Keywords: heterologous expression; recombinant pyranose 2-oxidase
Project no.: CEZ:AV0Z50200510 (CEP), ME 395
Funding provider: GA MŠk
Host item entry: International Symposium on the Genetics of Industrial Microorganisms /10./

Institution: Institute of Microbiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0146297

Permalink: http://www.nusl.cz/ntk/nusl-37177


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Research > Institutes ASCR > Institute of Microbiology
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