|
|
Isotachophoretic determination of ketone bodies and lactate in cattle serum
Vyskočilová, V. ; Pechová, A. ; Pantůčková, Pavla
The aim of the work was the optimization of isotachophoretic determination of lactate and ketone bodies (beta-hydroxybutyrate and acetoacetate) for veterinary medicine. The measurement of these metabolites is very important for the evaluation of metabolic diseases of cattle. The complete separation of these three analytes together with other possible blood components was achieved using 10 mmol/l hydrochloric acid with beta-alanine as a leading electrolyte at pH = 3.15 and 10 mmol/l sodium propionate as a terminating electrolyte. Limits of detection determined from the standard mixture for acetoacetate, lactate and beta-hydroxybutyrate were 3.75 mu mol/l, 3.96 mu mol/l and 7.46 mu mol/l, respectively.
|
|
|
Computer assisted development of a pneumatic electrospray nebulizer
Járvás, G. ; Szigeti, M. ; Grym, Jakub ; Foret, František ; Guttman, A.
In this study we demonstrate how computer simulations can assist the development of a novel microfabricated pneumatic electrospray nebulizer. The microdevice under consideration is designed for electrospray mass spectrometry interfacing without the need to fabricate an electrospray needle. This new interfacing strategy requires comprehensive evaluation of the differently designed chip layouts. The flow evaluation was supported by computational fluid dynamics simulations. It was proved with experimental validation of the simulation results that the developed models were practical tools for the numerical investigation of the separations-ESI-MS coupling.
|
|
|
THE WAYS OF ACTIVATING TP53
Polášková, Alena ; Helma, Robert ; Adámik, Matěj ; Hronešová, L. ; Holacka, K. ; Ballová, L. ; Brázdová, Marie
P53, AGENTS, CELLS,P53, AGENTS, CELLS,P53, AGENTS, CELLS,P53, AGENTS, CELLS
|
|
|
DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES
Petr, Marek ; Bažantová, Pavla ; Adámik, Matěj ; Kejnovská, Iva ; Dvořáková, Zuzana ; Vorlíčková, Michaela ; Pečinka, Petr ; Brázdová, Marie
In this work we performed DNA polymerase stop assays while using template DNA derived from promoter regions of VEGF and c-Myc proto-oncogenes under variety of experimental conditions. Partial stopping of DNA synthesis along the template strand was observed in the presence of K+ ions due to formation of stable G-quadruplex structures. In contrast, Na+ ions alone were unable to stabilize G-quadruplexes to stop the reaction at their site. These data suggest that K+ and Na+ ions, which are both known to stabilize G-quadruplexes, do this in different manner or extent.
|
|
|
P36 VOLTAMETRIC DETECTION OF DNA DELETION ON PENCIL ELECTRODE
Haroniková, Lucia ; Špaček, Jan ; Fojta, Miroslav
In this work, we present a new qualitative approach of detection of PCR products using electrochemistry on pencil electrodes. PCR products with an incorporated biotin-labeled dNTP (dCTP in this study) are detected via conjugated streptavidinealkaline phospatase. 1-Naphthyl phosphate (1-NP), which is dephosphorylated by alkaline phosphatase to release 1-naphthol, was used as a substrate in electrochemical detection of the PCR product. Voltammetric measurement on disposable pencil electrode is a very cheap and easy to use method. The system was optimized for plasmid DNA PCR product with potential to detect human genome DNA products and possible application in gene deletion monitoring.
|
|
|
ANALYSIS OF SEQUENCE SPECIFIC INTERACTIONS BETWEEN DNA AND P53 FAMILY PROTEINS BY ELISA, SLOT-BLOT AND EMSA
Adámik, Matěj ; Holanová, L. ; Navrátilová, Lucie ; Nygrinova, J. ; Pokorova, J. ; Petr, Marek ; Tichý, Vlastimil ; Brázdová, Marie
DNA-protein interactions of core domains of p53, p63 and p73, members of tumor suppressor p53 family, were investigated by multiple methods with regard to verifying the sequence specificity with which short target oligonucleotides/long DNA fragments can be recognized. The sequence specificity of core domains and wtp53 full protein binding to specific sequence in both types of DNA substrates was confirmed in solution, on surface and in gels by ELISA, slot-blot and EMSA.
|
|
| |
|
| |
|
| |
|
|
Identification of nosocomial infection by electrophoretic techniques
Kubesová, Anna ; Moravcová, Dana ; Tesařová, Marie ; Horká, Marie
The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic procedures to modern molecular biology based methods. However, conventional laboratory methods are time consuming, laborious, and they may provide both false positive or negative results, especially for closely related microorganisms. In this study, we suggest capillary electrophoresis techniques for differentiation and characterization of Methicillin-resistant and Methicillin-susceptible Staphylococcus aureus.
|
host ::
RSS.