National Repository of Grey Literature 6 records found  Search took 0.00 seconds. 
Influence of grapevine growing methods on quality of wine
Šprláková, Katarína ; Hrstka, Miroslav (referee) ; Vránová, Dana (advisor)
This thesis is concerned about the impact of ecological and integrated cultivation of vines for wine quality. The theoretical part describes the history, characteristics of the vine, the conditions that can affect the quality of grapes and the finished product. This work also describes the chemical composition of the fruit of the vine, the base phase of processing of grapes and wine production, from cider pressing to bottling processes and analyzes the chemical composition of wine. In the experimental part are monitored changes of chemical parameters (dry matter, alcohol, total and reducing sugars) in the musts during the process of fermentation. The musts come from grapes of Rulandské modré grown in a system of integrated and ecological production.
Autophagy in the heart
Šprláková, Katarína ; Hlaváčková, Markéta (advisor) ; Tomšů, Eva (referee)
Currently, it is growing evidence that autophagy is involved in the prevention of various diseases, which of course also includes heart diseases. This thesis is therefore aimed at clarifying the role of autophagy in the heart, especially during ischemia and subsequent reperfusion. Autophagy is a physiological cellular process by which the cell maintains homeostasis by eliminating long-lived proteins and damaged organelles. The role of autophagy during ischemia/reperfusion in the heart is complex. Predominantly it functions as a pro-survival pathway, because it protects the heart from ischemia or hypoxia. However, when triggered over, which happens during reperfusion, it may lead to cell death. In the heart autophagy is activated in response to various stimuli, such as decrease in ATP and subsequent activation of AMPK, protein Bnip3, reactive oxygen and nitrogen species, the opening of mitochondrial permeability transition pore, endoplasmic reticulum stress or unfolded protein response.
Expression of purinergic P2 receptors and their role in intracellular calcium signaling of neonatal pituitary cells
Šprláková, Katarína ; Stiborová, Marie (advisor) ; Ryšlavá, Helena (referee)
This diploma thesis focuses on study of the expression of purinergic receptors P2X and P2Y in the cells of neonatal adenohypofysis. Purinergic receptors are activated by binding of extracellular ATP and their activation leads to an increase in intracellular concentrations of calcium ions. The aim of this thesis was to determine by microfluorimetry method whether neonatal pituitary cells contain purinergic receptors. In addition, the influence of various agonists and antagonists of purinergic receptors on intracellular concentrations of ions Ca2+ in neonatal pituitary cells was monitored. The influence of extracellular ATP on secretion of a luteinizing hormone was observed by radioimmunoassay and it was compared with the effect of a hormone GnRH. It was found that neonatal pituitary cells are less sensitive to extracellular ATP than adult pituitary cells, but the relative sensitivity to other agonists of purinergic receptors is similar to the one of adult pituitary cells. In the mixed population of neonatal pituitary cells there were identified ATP- and 2 MeSATP- sensitive purinergic receptors P2X, as well as ADP-sensitive receptors P2Y. Further, the BzATP-sensitive receptors P2X7, PPADS-sensitive receptors P2X2 and BDBD-sensitive receptors P2X4 were identified. Finally, it was proved that...
Expression of purinergic P2 receptors and their role in intracellular calcium signaling of neonatal pituitary cells
Šprláková, Katarína ; Stiborová, Marie (advisor) ; Ryšlavá, Helena (referee)
This diploma thesis focuses on study of the expression of purinergic receptors P2X and P2Y in the cells of neonatal adenohypofysis. Purinergic receptors are activated by binding of extracellular ATP and their activation leads to an increase in intracellular concentrations of calcium ions. The aim of this thesis was to determine by microfluorimetry method whether neonatal pituitary cells contain purinergic receptors. In addition, the influence of various agonists and antagonists of purinergic receptors on intracellular concentrations of ions Ca2+ in neonatal pituitary cells was monitored. The influence of extracellular ATP on secretion of a luteinizing hormone was observed by radioimmunoassay and it was compared with the effect of a hormone GnRH. It was found that neonatal pituitary cells are less sensitive to extracellular ATP than adult pituitary cells, but the relative sensitivity to other agonists of purinergic receptors is similar to the one of adult pituitary cells. In the mixed population of neonatal pituitary cells there were identified ATP- and 2 MeSATP- sensitive purinergic receptors P2X, as well as ADP-sensitive receptors P2Y. Further, the BzATP-sensitive receptors P2X7, PPADS-sensitive receptors P2X2 and BDBD-sensitive receptors P2X4 were identified. Finally, it was proved that...
Autophagy in the heart
Šprláková, Katarína ; Hlaváčková, Markéta (advisor) ; Tomšů, Eva (referee)
Currently, it is growing evidence that autophagy is involved in the prevention of various diseases, which of course also includes heart diseases. This thesis is therefore aimed at clarifying the role of autophagy in the heart, especially during ischemia and subsequent reperfusion. Autophagy is a physiological cellular process by which the cell maintains homeostasis by eliminating long-lived proteins and damaged organelles. The role of autophagy during ischemia/reperfusion in the heart is complex. Predominantly it functions as a pro-survival pathway, because it protects the heart from ischemia or hypoxia. However, when triggered over, which happens during reperfusion, it may lead to cell death. In the heart autophagy is activated in response to various stimuli, such as decrease in ATP and subsequent activation of AMPK, protein Bnip3, reactive oxygen and nitrogen species, the opening of mitochondrial permeability transition pore, endoplasmic reticulum stress or unfolded protein response.
Influence of grapevine growing methods on quality of wine
Šprláková, Katarína ; Hrstka, Miroslav (referee) ; Vránová, Dana (advisor)
This thesis is concerned about the impact of ecological and integrated cultivation of vines for wine quality. The theoretical part describes the history, characteristics of the vine, the conditions that can affect the quality of grapes and the finished product. This work also describes the chemical composition of the fruit of the vine, the base phase of processing of grapes and wine production, from cider pressing to bottling processes and analyzes the chemical composition of wine. In the experimental part are monitored changes of chemical parameters (dry matter, alcohol, total and reducing sugars) in the musts during the process of fermentation. The musts come from grapes of Rulandské modré grown in a system of integrated and ecological production.

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