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Reasoning in the judgements of European courts
Vilímková, Veronika ; Tomášek, Michal (advisor) ; Kühn, Zdeněk (referee)
This thesis concerns the topic of legal reasoning in the judgements of European courts. The first part of the paper presents the analysis of the theoretical aspects of legal reasoning, specifically the legal obligation for this practice, as well as an examination of the origins and arguments for the existence of this obligation. Furthermore, the way that the legal reasonings are embedded in the law of the Czech Republic, and in EU legislation, is discussed and examined in detail. The final part of the paper consists of a comparison of legal reasonings of the Czech courts and the European Court of Justice. The relevant aspects of the legal reasoning are explained with regard to the practice of two European courts: European Court of Justice and Supreme Administrative Court of the Czech Republic, respectively.
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Organoids - the phenomenon of complex organ-like structures cultivation in vitro
Vilímková, Veronika ; Janečková, Lucie (advisor) ; Rothová, Olga (referee)
Pluripotent embryonal stem cells (ESCs) are unique for their ability to proliferate and differentiate into almost all cell types. In the presence of appropriate stimuli, they are able to initiate morphogenesis in vitro. Stem cells are able to differentiate into specific cell lines of a given tissue and to arrange three- dimensional (3D) structures. These so-called organoids are formed in a self-organizing process that mimics normal ESCs development. Organ development is a complex process that requires temporally and spatially ordered regulatory signals. 3D organization of these structures allows important cell-cell interactions. Organoid cultures are thus the area of a huge potential. Organoids are accessible to all standard experimental manipulations. They are therefore an ideal tool for the research of stem cells, signaling pathways, organogenesis and disease modeling. Finally, they may open up new avenues for regenerative medicine and gene therapy. Powered by TCPDF (www.tcpdf.org)
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Role of HIF-2alpha in erythropoiesis
Vilímková, Veronika ; Láníková, Lucie (advisor) ; Černý, Jan (referee)
The primary function of erythrocytes is transport of oxygen from lungs to various tissues of the body. Red blood cell mass, due to this important role, must be controlled at precise levels. The number of erythrocytes is primarilly increased by the glycoprotein hormon erythropoietin, which expression is controlled by HIF (hypoxia inducible factor). Transcriptional factor HIF consists of the two subunits, HIFα and HIFβ. Under normoxic conditions, alfa subunit of HIF is hydroxylated by PHD protein. This hydroxylation provides a recognition motif for the VHL protein, a part of an E3 ubiquitin ligase complex that targets hydroxylated HIF for proteasomal degradation. Under hypoxic conditions, the degradation is inhibited. The alfa subunit is translocated to the nucleus, where binds the beta subunit and regulates gene expression. HIF pathway regulates a broad spectrum of cellular functions - energy metabolism, angiogenesis, apoptosis and many others. This diploma thesis is focused on HIF2α and its role in erythropoiesis. In this present study, we used CRISPR/Cas9 technology and created HEL (human erythroleukemia) cell line with knock-out of the gene for HIF2α (EPAS1). To reveal the role of HIF2α, we used specific HIF2α inhibitor in order to block its function in HEL cell line. We also tested this...
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Organoids - the phenomenon of complex organ-like structures cultivation in vitro
Vilímková, Veronika ; Janečková, Lucie (advisor) ; Rothová, Olga (referee)
Pluripotent embryonal stem cells (ESCs) are unique for their ability to proliferate and differentiate into almost all cell types. In the presence of appropriate stimuli, they are able to initiate morphogenesis in vitro. Stem cells are able to differentiate into specific cell lines of a given tissue and to arrange three- dimensional (3D) structures. These so-called organoids are formed in a self-organizing process that mimics normal ESCs development. Organ development is a complex process that requires temporally and spatially ordered regulatory signals. 3D organization of these structures allows important cell-cell interactions. Organoid cultures are thus the area of a huge potential. Organoids are accessible to all standard experimental manipulations. They are therefore an ideal tool for the research of stem cells, signaling pathways, organogenesis and disease modeling. Finally, they may open up new avenues for regenerative medicine and gene therapy. Powered by TCPDF (www.tcpdf.org)
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Reasoning in the judgements of European courts
Vilímková, Veronika ; Tomášek, Michal (advisor) ; Kühn, Zdeněk (referee)
This thesis concerns the topic of legal reasoning in the judgements of European courts. The first part of the paper presents the analysis of the theoretical aspects of legal reasoning, specifically the legal obligation for this practice, as well as an examination of the origins and arguments for the existence of this obligation. Furthermore, the way that the legal reasonings are embedded in the law of the Czech Republic, and in EU legislation, is discussed and examined in detail. The final part of the paper consists of a comparison of legal reasonings of the Czech courts and the European Court of Justice. The relevant aspects of the legal reasoning are explained with regard to the practice of two European courts: European Court of Justice and Supreme Administrative Court of the Czech Republic, respectively.
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