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Climate crisis in Czech online media
Málek, Albert ; Vochocová, Lenka (advisor) ; Podzimek, Jan (referee)
The climate crisis is one of the greatest risks society is facing nowadays. How the media present the issue is therefore crucial. However, many different actors are trying to influence the media communication of the climate crisis in their favour. The aim of this thesis was to use a qualitative content analysis method to identify the main actors to whom the media attribute responsibility for addressing the climate crisis, and then to analyse the motives that lead the media to create these constructions. Therefore, media portrayals of the climate crisis were viewed through the lens of the political economy of communication, which allows for the examination of aspects of how media products influence the final form of the media products, taking into account the power and economic context in which the media operate. Specifically, the thesis looked at articles related to the climate crisis published in the Czech online media iROZHLAS, Aktuálně.cz, Forbes and Alarm. These were articles published in the period bounded by the global climate strike (15th March 2019) and the awarding of a verdict in the climate lawsuit against the Czech Republic (15th June 2022). The findings of this thesis show that most of the media outlets examined produced constructions that shifted responsibility for addressing the...
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Creation of material for chemistry teaching in virtual reality
Málek, Albert ; Míka, Luděk (advisor) ; Šmejkal, Petr (referee)
Modern education is developing dynamically along with new technologies. One of the main aspects of this development has been the massive expansion of the consumption of online video content, especially on social networks. Another and similarly major influence may be virtual reality (VR) in the near future. This thesis combines both of these aspects and sets as one of its goals the creation of an educational VR video available on the social network YouTube. The theoretical part of the thesis first describes aspects of the use of VR and video not only in chemistry education. It also summarizes the existing knowledge about what a good educational VR video should look like. This knowledge then served as a basis for the search of available educational VR videos and for its actual production. In less than six minutes, the video produced as part of this work introduces the viewer to the workings of the global carbon cycle and its connection to climate change. Working in the 3D modelling program Blender was essential to the creation of the video, which allowed for the inclusion of more sophisticated visual elements. The video was tested with 15 respondents, aged secondary and primary school students, who, after watching it, completed a knowledge test to check the transfer and understanding of the...
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Clinically relevant protein-protein interactions participating in process of bacterial pathogenesis B. pertusis
Málek, Albert ; Šulc, Miroslav (advisor) ; Černá, Věra (referee)
Whooping cought in human population was strongly suppressed during the 20th century. But in the past few years, the incidence of whooping cough began to rise. The origin of this disease is a pathogenic gram-negative bacteria Bordetella pertussis, which is becoming resistang to currecently used antibiotics or vaccination. B. pertussis attacks human respiratory system. One of it's virulent factors is adenylate cyclase toxin (ACT). It's secreted extracellularly from bacteria and binds to cytoplasmic membrane of host cells and translocates adenylatecyclase domain (dAC) to cytosol. This enzymatic domain is activated by non- covalent interaction with eukaryotic Calmodulin (CaM). After it's activation, dAC in high concentrations synthesizes cAMP, triggering host cell's apoptosis. We studied protein interaction of dAC with CaM by the PIXL method (Photo Induced Cross Linking) and mass spectrometry (MS). Mutant of dAC, with photo-methionine (pM), incorporated in position of Leucine 240 (dACL240pM) was expressed in transformed E.coli cells B834 in mineral medium containing pM. Expressed protein was isolated by affinite chromatography and characterized by MS (determined incorporation of pM was approximately 50 % in the final protein preparation). We performed a photochemical cross-linking with isolated...
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Clinically relevant protein-protein interactions participating in process of bacterial pathogenesis B. pertusis
Málek, Albert ; Šulc, Miroslav (advisor) ; Černá, Věra (referee)
Whooping cought in human population was strongly suppressed during the 20th century. But in the past few years, the incidence of whooping cough began to rise. The origin of this disease is a pathogenic gram-negative bacteria Bordetella pertussis, which is becoming resistang to currecently used antibiotics or vaccination. B. pertussis attacks human respiratory system. One of it's virulent factors is adenylate cyclase toxin (ACT). It's secreted extracellularly from bacteria and binds to cytoplasmic membrane of host cells and translocates adenylatecyclase domain (dAC) to cytosol. This enzymatic domain is activated by non- covalent interaction with eukaryotic Calmodulin (CaM). After it's activation, dAC in high concentrations synthesizes cAMP, triggering host cell's apoptosis. We studied protein interaction of dAC with CaM by the PIXL method (Photo Induced Cross Linking) and mass spectrometry (MS). Mutant of dAC, with photo-methionine (pM), incorporated in position of Leucine 240 (dACL240pM) was expressed in transformed E.coli cells B834 in mineral medium containing pM. Expressed protein was isolated by affinite chromatography and characterized by MS (determined incorporation of pM was approximately 50 % in the final protein preparation). We performed a photochemical cross-linking with isolated...
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