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BacSeqer: Read simulator for bacterial RNA-Seq
Fialová, Adéla ; Sedlář, Karel
The RNA-Seq method has become a key tool in transcriptomics, providing deep insight into the genetic functioning of organisms. It is essential for understanding how genes are expressed, for discovering new gene variants and for understanding pathologies at the genetic level. RNA-Seq simulators are great for testing and validating bioinformatics tools, they offer a way to compare different computational algorithms and allow examining different sequencing protocols, parameter settings and sample sizes to ensure the most efficient use of resources. This study introduces BacSeqer, an RNA-Seq simulator specifically designed for bacterial transcriptomes with the aim of producing data that faithfully resembles those obtained in real experiments. It allows to take into account the specificities of the bacterial genome, especially an existence of only exons and their organization in operons that are not normally present in eukaryotes.
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Read simulator for bacterial RNA-Seq
Fialová, Adéla ; Jurečková, Kateřina (referee) ; Sedlář, Karel (advisor)
This thesis is dedicated to the analysis of the bacterial genome, measurement of its expression mainly by RNA-Seq technology and simulation of RNA-Seq data. The first part of the thesis provides a theoretical background on the structure of the bacterial genome, its expression and methods to study it, including modern sequencing techniques. Several selected simulators of RNA-Seq data are also mentioned. In the second part, the actual simulator implementation is presented, designed to take into account the characteristics of the bacterial genome, in particular the presence of operons.
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Comparison of Zuzana Čaputová's media image on the most popular Czech and Slovak news servers in a half year to one year since she was elected Slovak President
Fialová, Adéla ; Jirků, Jan (advisor) ; Cebe, Jan (referee)
In March 2019 the 5th direct presidential elections were held in Slovakia. In the first round there were 13 candidates running for the office. The winner after the second round held on March 30th was Zuzana Čaputová. With 58,4 percent of all valid votes she became the first female president of The Slovak republic on June 15. This thesis goes into the comparison of Zuzana Čaputová's media image since being elected on the most visited news web sites in The Czech republic and Slovakia. The goal of the thesis is to compare the quantity and content of information published in both countries and to track possible changes in the media narrative and what has caused them. Using the quantitative method of content analysis and qualitative method of frame analysis of relevant articles published on news websites Novinky.cz and Aktuality.sk I want to monitor the decline, stagnation or rise of media interest in the persona of Zuzana Čaputová in the selected time period. This will help to describe the media image of the president on the Czech and Slovak media scene. The source articles are attached to the thesis.
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Nicolae Ceauşescu and his stance towards the invasion of the Warsaw pact armies to Czechoslovakia in 1968
Fialová, Adéla ; Kocián, Jiří (advisor) ; Tejchman, Miroslav (referee)
The subject of this bachelor thesis are the policies of Romania in the 1960's, which can be characterized by efforts to gain independence on the Soviet regime. The thesis focuses especially on the former general secretary of the Romanian communist party Nicolae Ceauşescu's stance towards the invasion of five Warsaw pact countries' armies to Czechoslovakia. In the form of historical analysis the thesis explores the emergence of the autonomous position of Romania among other socialist states, the events preceding the intervention and finally both domestic and international consequences of the Romanian approach to the invasion to Czechoslovakia. In it's final chapters the thesis sketches out the development of Ceauşescu's policies up until the early 1970's. The objective of the thesis is to find out what were the reasons for rejecting the intervention, why Romania did not take part in it and what were the impacts of this step.
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The long-term trends in numbers and distribution of birds in the Czech Republic
Gebauer Fialová, Adéla ; Musil, Petr (advisor) ; Fuchs, Roman (referee)
The long-term changes in distribution and numbers of bird species in the Czech Republic were analysed using published data from Breeding Birds Atlases in the Czech Republic (1973-77, 1985-89 and 2001-03). Each species were categorized by taxonomical group, migratory strategies, biogeography type, breeding habitat, breeding population size in Europe and in the Czech Republic a body size. The changes in numbers and changes in distribution were inter-correlated Increase in distribution as well as in numbers were recorded in rare and large species, in species increasing in whole Europe and in bird species protected under Czech legislation. Interesting pattern in population was found in waterbirds, where piscivorous species are increasing, whereas other (mostly grebes, ducks etc.) species are decreasing.
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Educational attitudes of foster parents.
FIALOVÁ, Adéla
Foster care is a form of foster care and it regulates family law (Foster care is a form of foster care and it regulates family law). Foster care controls and guarantes the State. Children suitable for foster care are children which are legally free, and therefore can not be entrusted to adoption. To foster care are usually placed older children or children with disabilities. Foster care expires at legal age of the child. If the child prepares for a future job, foster care can take up to 26 years of age. Foster care can be performed in groups or individually. In the first chapters on the topic: educational attitudes of foster parents I deal with family and the atmosphere in it. In the other chapters I describe forms of foster care - custody, guardianship, adoption or foster care and adoption, where I also mention the history of foster care and intermediation of adoption and foster care. One chapter I deal to biological parents of the children because foster children in foster care and biological family have the right to be in contact. In a few chapters I deal to education, where I write in detail about educational resources and of reward and punishment. In addition, in the chapter on education I deal with parental attitudes toward child. In half page I deal chapter titled rules, where I mention the importance of the rules and the various areas in which there are rules. One chapter I devote to conflicts and their solutions, where I write about the different ways to solve the conflict. Because they are placed in foster care the children with disabilities, at the end of the theoretical part of the thesis I deal with chapter named: the children with disabilities in foster families, and specifically down syndrome and autism. I choosed a qualitative method for the research project and I performed interviews with respondents with half structured interview. The respondents were six foster families. I recorded interviews on the recorder with the consent of the respondents and then transcribed . The only criterion to decide on the respondents was that the respondents must be currently foster parents and be in foster care for at least one foster child. In the research section I have chosen the following objectives: 1. The objective is to establish a conscious and unexplored educational attitudes of foster parents 2. The operational objective is to capture the context changes and the development of educational attitudes of foster parents. To achieve these objectives, I formulated the following research questions: 1.Which styles of education do foster parents use in the upbringing of the children? Half of the respondents agreed that in education are important rules and boundaries that should be respected. In the education of children with foster parents attempt to a democratic approach. 2. Which rewards or punishments do foster parents use? Foster parents report that violations of the rules are punished by a ban on popular activities, such as television or computers. Foster parents agreed that physical punishment don´t use, except for an occasional slap. 3. Do exist differences in education of biological children and conferred on the children in foster care? All foster families have agreed that they don´t make differences in education, biological and conferred on the children. But also the majority of foster parents think that generally foster parents make the differences between biological children and the children entrusted, for example, as clothes, and that for some it is a lot to see. Two guardians indicated that there should be a difference in approach, in the sense that not everything should be entrusted children to say straight. The conclusion of my bachelor thesis I finish discussion, where I return again to the results of the research part of the thesis. This thesis provides an authentic experience of foster parents
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